BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,861	T→C	41.1%	intergenic (‑944/‑224)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,861	0	T	C	41.1%	96.7 / 243.6	201	intergenic (‑944/‑224)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (67/51); new base C (43/39); total (111/90)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 202 total aligned reads displayed.

ATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCA  >  CP000730/1929721‑1929996

    aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑Taa                                                                                                                                       >  1:82037/1‑141 (MQ=255)

        tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTg                                                                                                                                 >  1:69780/1‑141 (MQ=255)

         aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGa                                                                                                                                <  1:687652/141‑1 (MQ=255)

           aCGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATg                                                                                                                              <  1:1148875/141‑1 (MQ=255)

             gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCt                                                                                                                            <  1:1583504/141‑1 (MQ=255)

              aTTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑T‑GTGATGCtt                                                                                                                           <  1:1458031/141‑1 (MQ=255)

              aTTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCtt                                                                                                                           <  2:438635/141‑1 (MQ=255)

              aTTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCtt                                                                                                                           <  1:832439/141‑1 (MQ=255)

                   aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTAtttt                                                                                                                      >  2:110052/1‑141 (MQ=255)

                    tCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTc                                                                                                                     >  1:1503349/1‑141 (MQ=255)

                    tCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGGATATTTGCCGATGATATAATAGTAGAGGCGCCTAGCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTc                                                                                                                     >  1:163598/1‑141 (MQ=255)

                     cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCg                                                                                                                    <  2:401195/141‑1 (MQ=255)

                      tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGt                                                                                                                   >  2:793150/1‑141 (MQ=255)

                          aGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTAt                                                                                                                         >  2:107119/1‑131 (MQ=255)

                          aGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTAt                                                                                                                         <  1:107119/131‑1 (MQ=255)

                           gCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGGTATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTcaca                                                                                                              >  2:32926/1‑141 (MQ=255)

                             tAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCAcatc                                                                                                            <  2:770310/141‑1 (MQ=255)

                                      ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCa                                                                                                   >  1:642179/1‑141 (MQ=255)

                                      ataACTTATCAGAAGCAAGTGACTGAGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCa                                                                                                   >  1:1374335/1‑141 (MQ=255)

                                        aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                                 >  2:1504494/1‑141 (MQ=255)

                                            tATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGtcatc                                                                                             <  2:1206584/141‑1 (MQ=255)

                                             aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGtcatca                                                                                            <  2:884032/141‑1 (MQ=255)

                                                aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAgccactgatg                                                                                         <  1:110052/141‑10 (MQ=255)

                                                aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGccactgatg                                                                                         <  1:1121115/141‑7 (MQ=255)

                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTACT‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                      >  1:1564238/14‑141 (MQ=255)

                                                  aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                       >  2:24189/1‑141 (MQ=255)

                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                     >  1:949039/13‑141 (MQ=255)

                                                   aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                      >  2:1002215/1‑141 (MQ=255)

                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                  >  2:781169/1‑141 (MQ=255)

                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTCC‑T‑TTGCTACTCATGTTCTTTCTCATCATAGCACCAGTCAGCAGTGGCTGTGctg                                                                                <  2:1345461/134‑3 (MQ=255)

                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                >  1:377700/8‑139 (MQ=255)

                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                >  1:834694/8‑139 (MQ=255)

                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                               <  1:317160/135‑4 (MQ=255)

                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                               <  1:1525531/135‑4 (MQ=255)

                                                          gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                               >  1:273451/1‑141 (MQ=255)

                                                          gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                               <  2:862931/141‑1 (MQ=255)

                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                             >  1:1127805/5‑136 (MQ=255)

                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                             <  1:404582/137‑6 (MQ=255)

                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg                                                                           <  2:759533/139‑8 (MQ=255)

                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                          >  2:754787/2‑141 (MQ=255)

                                                               tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                         <  2:185544/141‑2 (MQ=255)

                                                                gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtt                                                                         <  2:1448315/141‑1 (MQ=255)

                                                                 cTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGa                                                                                                                                <  2:1609507/85‑1 (MQ=255)

                                                                 cTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGa                                                                                                                                >  1:1609507/1‑85 (MQ=255)

                                                                 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                       <  2:604171/141‑4 (MQ=255)

                                                                  ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                      <  2:1540901/141‑5 (MQ=255)

                                                                   tgtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                     >  2:62587/13‑139 (MQ=255)

                                                                     tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                                                                    <  1:611918/141‑1 (MQ=255)

                                                                        ttttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGCCATCAGTGGCTGTGCCATTGCGTTTTTTTcgt                                                                 <  2:895126/140‑3 (MQ=255)

                                                                        atTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                 >  2:1315681/1‑141 (MQ=255)

                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                       >  2:953128/5‑128 (MQ=255)

                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                       <  1:953128/127‑4 (MQ=255)

                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                             >  2:419108/5‑141 (MQ=255)

                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑A‑TTGCTAATCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                             >  2:656539/5‑141 (MQ=255)

                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTACT‑TTGCTAATCATTTTCGTTCACATCATAACACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                             >  2:518595/5‑141 (MQ=255)

                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGCAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                             <  1:782490/137‑1 (MQ=255)

                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                           <  1:1543040/139‑1 (MQ=255)

                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTA‑CTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                           >  1:1257520/3‑141 (MQ=255)

                                                                               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTACT‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                         >  2:1652596/1‑140 (MQ=255)

                                                                               gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                          <  2:199270/141‑1 (MQ=255)

                                                                               gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                          <  1:172675/141‑1 (MQ=255)

                                                                                  gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                       <  1:753997/141‑1 (MQ=255)

                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTA‑CTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                     >  2:863701/2‑141 (MQ=255)

                                                                                   atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                      >  1:562697/1‑141 (MQ=255)

                                                                                    tataATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGGAGAGAGGAGGT‑GTA‑TGA‑G‑GTGATGCTTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGACATTGCGTTTTTTTCTTATTGGCTAAGTa                                                     <  2:319493/141‑1 (MQ=255)

                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                    >  2:748652/1‑140 (MQ=255)

                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                    >  1:224511/1‑140 (MQ=255)

                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                    <  2:260133/141‑2 (MQ=255)

                                                                                       aataatAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                 >  2:578323/1‑141 (MQ=255)

                                                                                        ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                <  2:939624/137‑1 (MQ=255)

                                                                                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                            >  1:586757/3‑105 (MQ=255)

                                                                                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                           <  2:1170798/141‑4 (MQ=255)

                                                                                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                           >  1:483866/1‑138 (MQ=255)

                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                         <  1:1173820/141‑6 (MQ=255)

                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                         <  1:333207/141‑6 (MQ=255)

                                                                                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                        <  2:622170/141‑7 (MQ=255)

                                                                                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGATGGGG‑TAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                        >  1:798539/1‑135 (MQ=255)

                                                                                                  cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTACT‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                      >  1:476466/1‑133 (MQ=255)

                                                                                                   gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGCGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                      >  2:1102600/1‑141 (MQ=255)

                                                                                                   gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                      >  2:1261408/1‑141 (MQ=255)

                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                     <  2:377700/141‑10 (MQ=255)

                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                     <  1:1499478/141‑10 (MQ=255)

                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCATTAC‑T‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                 >  2:14523/1‑102 (MQ=255)

                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGT‑GCA‑TTACT‑TTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGGAAACGCaacaaataattaa                                   >  1:389714/1‑130 (MQ=255)

                                                                                                        tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGACGCAATACAAAATAGGTg                                 >  1:627953/1‑141 (MQ=255)

                                                                                                              aGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                           <  1:1058421/141‑1 (MQ=255)

                                                                                                                gCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg                         >  2:398026/1‑141 (MQ=255)

                                                                                                                   tCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACACAATAGGTAACATATACCCg                      >  2:593632/1‑141 (MQ=255)

                                                                                                                       tttAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                  <  2:82037/141‑1 (MQ=255)

                                                                                                                         tAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCaa                >  2:307028/1‑141 (MQ=255)

                                                                                                                          aGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATGTAGCCGCACCAAt               >  2:136528/1‑141 (MQ=255)

                                                                                                                          aGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAt               >  2:1034142/1‑141 (MQ=255)

                                                                                                                          aGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAt               <  1:1087759/141‑1 (MQ=255)

                                                                                                                          aGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAt               <  2:184202/141‑1 (MQ=255)

                                                                                                                           gACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa              <  2:219729/141‑1 (MQ=255)

                                                                                                                             cGCAGAGAGGAGGT‑GTA‑TAA‑G‑TTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaa            <  2:148404/141‑1 (MQ=255)

                                                                                                                              gCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa           >  1:71364/1‑141 (MQ=255)

                                                                                                                              gCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa           <  1:1004352/141‑1 (MQ=255)

                                                                                                                                     ggaggT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt    >  2:1583716/1‑141 (MQ=255)

                                                                                                                                       aggT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCa  >  1:1449911/1‑141 (MQ=255)

ATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGT‑GTA‑TAA‑G‑GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCA  >  CP000730/1929721‑1929996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`