Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 9,710 T→C 6.1% intergenic (+15/+72) gyrA → / ← nnrD DNA topoisomerase (ATP‑hydrolyzing) subunit A/possible carbohydrate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007309,7100TC 6.1% 222.6 / 5.0 82intergenic (+15/+72)gyrA/nnrDDNA topoisomerase (ATP‑hydrolyzing) subunit A/possible carbohydrate kinase
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base C (2/3);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

GCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATAC  >  CP000730/9576‑9848
                                                                                                                                             |                                                                                                                                          
gCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATGAAAAATAAGAGTTCCCtat                                                                                                                                      >  2:673138/1‑141 (MQ=255)
gCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtat                                                                                                                                      >  1:545541/1‑141 (MQ=255)
 cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                     >  1:148936/1‑141 (MQ=255)
 cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                     <  1:272640/141‑1 (MQ=255)
 cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                     <  2:407086/141‑1 (MQ=255)
 aaataaataaaaaaGAGATTGATTCAGAAGAAAATGATGAAGATGGAAGTAGTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACCACAATCATC‑‑‑‑‑‑AGATGAAAAAAAAAAAAAAATAAGACTTCCCtata                                                                                                                                     <  2:407087/129‑1 (MQ=255)
  aaTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTatat                                                                                                                                    <  1:289738/141‑1 (MQ=255)
   aTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATg                                                                                                                                   >  2:280041/1‑141 (MQ=255)
    ttCATAGTGAAATGATTGATTAAGAAGAAAATGATGAAGATGGACGAATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGt                                                                                                                                  <  2:459943/141‑1 (MQ=255)
    ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCtacatat                                                                                                                                  <  1:377609/141‑6 (MQ=255)
      cATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAg                                                                                                                                >  1:91367/1‑141 (MQ=255)
       aTACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAACCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAATAAGACTCCCCTACATATAggg                                                                                                                              <  2:345846/141‑1 (MQ=255)
          cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                            >  2:668831/1‑141 (MQ=255)
          cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                            >  1:299658/1‑141 (MQ=255)
              aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCt                                                                                                                        <  1:18345/141‑1 (MQ=255)
                tgattgatTCAGAAGAAAATGATGAAGATGGACGGATTGAAGTAAGACAAGATTCCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTa                                                                                                                      >  2:463343/1‑141 (MQ=255)
                  attgattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAtt                                                                                                                    >  1:290292/1‑141 (MQ=255)
                      attCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATCCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTa                                                                                                                <  2:86626/141‑1 (MQ=255)
                       ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTAt                                                                                                               <  1:481622/141‑1 (MQ=255)
                        tCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATg                                                                                                              >  1:491916/1‑141 (MQ=255)
                         cAGAAGAAACTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGc                                                                                                             >  1:509542/1‑141 (MQ=255)
                         aagaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATTATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGTGGAGGCTTATTTTTATGc                                                                                                             <  2:50210/140‑1 (MQ=255)
                           gaagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTGTATGTAGGGGAGTCTTATTTTTATGCTa                                                                                                           >  2:514475/1‑141 (MQ=255)
                           gaagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTa                                                                                                           >  1:124284/1‑141 (MQ=255)
                           gaagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTa                                                                                                           >  2:676817/1‑141 (MQ=255)
                            aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAg                                                                                                          <  2:599203/141‑1 (MQ=255)
                                 aaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGt                                                                                                     <  2:687092/141‑1 (MQ=255)
                                   tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGGTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                   >  2:694281/1‑141 (MQ=255)
                                   tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                   >  2:520021/1‑141 (MQ=255)
                                   tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                   >  2:542741/1‑141 (MQ=255)
                                      tgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGc                                                                                                >  1:55267/1‑141 (MQ=255)
                                      tgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGc                                                                                                <  1:410816/141‑1 (MQ=255)
                                        aaGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCtt                                                                                              <  1:280041/141‑1 (MQ=255)
                                        aaGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCAAC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCtt                                                                                              <  2:93269/141‑1 (MQ=255)
                                          gATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCAGC‑‑‑‑‑‑AGTTTAAGTGTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTa                                                                                            >  1:314901/1‑141 (MQ=255)
                                           aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                           <  1:98964/141‑1 (MQ=255)
                                            tGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACt                                                                                          <  2:100620/141‑1 (MQ=255)
                                            tGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTACATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACt                                                                                          >  2:272994/1‑141 (MQ=255)
                                                   aTTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCa                                                                                   >  1:551939/1‑141 (MQ=255)
                                                      gAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATTAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATg                                                                                <  1:63860/141‑1 (MQ=255)
                                                      gAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTGCCCTATAGTTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATg                                                                                <  1:336060/141‑1 (MQ=255)
                                                      gAAGTAAGACAAGATTTAATGGGTCGTGGTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTGTACtata                                                                                       >  2:533063/1‑141 (MQ=255)
                                                        aGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGAt                                                                              <  2:509542/141‑1 (MQ=255)
                                                        aGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGAt                                                                              >  1:213495/1‑141 (MQ=255)
                                                            aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                          <  2:35312/141‑1 (MQ=255)
                                                             gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                         >  1:220294/1‑141 (MQ=255)
                                                                  gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                    >  2:144643/1‑141 (MQ=255)
                                                                      tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAGAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                >  2:298567/1‑141 (MQ=255)
                                                                      tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                <  1:377274/141‑1 (MQ=255)
                                                                       cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                               >  2:694984/1‑141 (MQ=255)
                                                                       cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                               >  2:51669/1‑141 (MQ=255)
                                                                       cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                               >  2:315063/1‑141 (MQ=255)
                                                                       cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTACTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                               >  2:315064/1‑141 (MQ=255)
                                                                         tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAt                                                                                                                     <  1:545936/85‑1 (MQ=25)
                                                                         tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAt                                                                                                                     >  2:545936/1‑85 (MQ=25)
                                                                              cGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAt                                                        <  2:409677/141‑1 (MQ=255)
                                                                                   tGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGttt                                                   >  2:400594/1‑141 (MQ=255)
                                                                                    gaagaaGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTc                                                  <  2:334878/141‑1 (MQ=255)
                                                                                         agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                             <  2:591854/141‑1 (MQ=255)
                                                                                          gaTATACAACAATTATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                            <  2:593113/141‑1 (MQ=255)
                                                                                          gaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                            <  1:178422/141‑1 (MQ=255)
                                                                                             ataCAACAATCATC‑‑‑‑‑‑AGTTGAAGAATAATTAAAAATAAGACTTCCCTATATGTAGGGGAGACTTATTTGTATGCTAGAAAGTAATGCTTTACGATATTCACTTATTAGTATTGACTAACTTCCAATTTGTTACATAGCGTaa                                         >  2:43598/1‑141 (MQ=255)
                                                                                             ataCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACta                                                                                         <  1:359478/93‑1 (MQ=255)
                                                                                             ataCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACta                                                                                         >  2:359478/1‑93 (MQ=255)
                                                                                                 aacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTa                                     <  2:220294/141‑1 (MQ=255)
                                                                                                 aacaaTCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTa                                     >  2:201362/1‑141 (MQ=255)
                                                                                                       catt‑‑‑‑‑‑aGATGAAGAATAATAAAAAATAAGTCTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTACGGTATTTCAt                               <  2:502718/141‑1 (MQ=255)
                                                                                                                   aTGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATaa                         >  1:173579/1‑141 (MQ=255)
                                                                                                                       agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                     >  1:402479/1‑141 (MQ=255)
                                                                                                                          ataataAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTGGAAAGTAATTCTTTACTATATTCAATGATTAGTAATGTCTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGa                  <  2:230929/141‑1 (MQ=255)
                                                                                                                          ataataAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGa                  <  2:551939/141‑1 (MQ=255)
                                                                                                                             ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg               <  1:520021/141‑1 (MQ=255)
                                                                                                                              taaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggt              >  1:521312/1‑141 (MQ=255)
                                                                                                                                aaaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtg             >  2:423290/1‑141 (MQ=255)
                                                                                                                                    aaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac         >  1:707866/1‑141 (MQ=255)
                                                                                                                                     aTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTatat                                                                                      <  1:62279/63‑1 (MQ=255)
                                                                                                                                     aTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTatat                                                                                      >  2:62279/1‑63 (MQ=255)
                                                                                                                                       aaGACTCCCCTACATATAGGGAAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcaccac      >  1:238205/1‑141 (MQ=39)
                                                                                                                                        aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca     >  2:155205/1‑141 (MQ=255)
                                                                                                                                        aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca     >  2:438009/1‑141 (MQ=255)
                                                                                                                                           cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac  <  1:43598/141‑1 (MQ=255)
                                                                                                                                           cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac  <  2:52429/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATAC  >  CP000730/9576‑9848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: