| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,268,572 | A→T | 12.3% | intergenic (‑460/+78) | USA300HOU_2142 ← / ← USA300HOU_2143 | hypothetical protein/possible HAD superfamily hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,268,572 | 0 | A | T | 12.3% | 144.9 / 13.1 | 49 | intergenic (‑460/+78) | USA300HOU_2142/USA300HOU_2143 | hypothetical protein/possible HAD superfamily hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (21/22); new base T (2/4); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
GAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTATGC > CP000730/2268438‑2268712 | gAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGaaa > 2:158996/1‑141 (MQ=255)gAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGaaa < 1:506058/141‑1 (MQ=255)gAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGaaa > 1:670180/1‑141 (MQ=255) aaaTATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGaaaa < 1:584480/141‑1 (MQ=255) ttattCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtc < 2:531697/141‑4 (MQ=38) ttattCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtc < 2:140864/141‑4 (MQ=38) tCAAAATCAATGAATTTCCTATCTTATTAGTTTAAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGACGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTAc < 2:536343/141‑1 (MQ=255) aaaaTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtcttactg < 2:546502/141‑10 (MQ=37) aaTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGCGCAGTAAAGTTCTTTTCTATTAGAAAAtgtcttactgct < 2:604161/141‑12 (MQ=37) aTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGCTGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGctc < 1:657463/141‑1 (MQ=255) gAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTa > 1:64795/1‑141 (MQ=255) aTTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACa < 2:225609/141‑1 (MQ=255) aGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTAATCAACTAt > 2:490904/1‑141 (MQ=255) tttAACAATATTTATTCTAATAGTGATTGTCTAATTAAATTTAGATGATTCAAATATTTATACGCATGCAAAAGATCAGT‑CTTCCATTTTCTAATAGAAAATAATTTTACTGCTC‑TTTGTACATTACTCCTCAACTATTGt < 2:152042/141‑1 (MQ=255) tAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaa < 1:421298/141‑1 (MQ=255) aaTATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaaatta < 2:696879/141‑1 (MQ=255) atttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaaattaaat > 2:633382/1‑141 (MQ=255) tttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGctc‑t < 1:568620/106‑1 (MQ=255) tttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGctc‑t > 2:568620/1‑106 (MQ=255) tattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGt < 1:449268/129‑1 (MQ=255) tattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGt > 2:449268/1‑129 (MQ=255) tctcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataataaa > 2:566730/1‑141 (MQ=255) tctcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataataaa > 2:83310/1‑141 (MQ=255) tcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataataaata < 1:70435/141‑1 (MQ=255) agagTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTa < 1:619729/141‑1 (MQ=255) agagTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTa < 2:277579/141‑1 (MQ=255) gcgTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAACAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTACATAATAAATAACTAc > 2:317351/3‑141 (MQ=255) ttGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAt > 2:148176/1‑141 (MQ=255) ttGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAt < 1:617559/141‑1 (MQ=255) gTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAttt > 1:339007/1‑141 (MQ=255) tCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAtttt < 1:541769/141‑1 (MQ=255) ccAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTtta > 2:208031/1‑141 (MQ=255) aaTTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTttatt > 1:249662/1‑141 (MQ=255) aaTATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCa > 2:633735/1‑141 (MQ=255) taGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTa > 1:317154/1‑141 (MQ=255) taGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTa > 1:351091/1‑141 (MQ=255) gatTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑‑‑‑‑‑‑‑‑TTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGc < 2:157190/141‑1 (MQ=255) ttATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTaa < 2:26184/141‑1 (MQ=255) ttATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTaa > 1:669545/1‑141 (MQ=255) tataAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTACTaaa > 2:577157/1‑141 (MQ=255) tACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGGTAAATATTTtgc < 2:271141/141‑1 (MQ=255) tACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTtgc < 2:329676/141‑1 (MQ=255) tACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTtgc < 1:168498/141‑1 (MQ=255) acaaaGAGCAGTAAAGTTATTTTCTATTAGAAAATGTC‑TTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTtgctg > 1:618050/3‑141 (MQ=25) agagCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCAGCGcc > 2:575202/1‑141 (MQ=255) agtaaagttaTTTTCTATTAGAAAATGTC‑TTACTGCTCTTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc > 1:215781/10‑141 (MQ=25) aCATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCAt < 1:603634/141‑1 (MQ=255) aCATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCAt < 2:358695/141‑1 (MQ=255) cTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCCGCTCAAAAGTACTCCGGTAACTTTTTGGCTGCTCCATCCTCTTCATTCGTGTa > 1:670742/1‑141 (MQ=255) aaTAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTATg > 1:137881/1‑141 (MQ=255) aTAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTATGc < 1:633382/141‑1 (MQ=255) | GAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTATGC > CP000730/2268438‑2268712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |