Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,636,962 A→T 6.6% Y271* (TAT→TAA gntP ← GntP family gluconate:proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,636,9620AT 6.6% 187.3 / 4.4 61Y271* (TAT→TAAgntPGntP family gluconate:proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base T (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

ATAACATCATTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTAAA  >  CP000730/2636823‑2637102
                                                                                                                                           |                                                                                                                                            
aTAACATCATTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCatat                                                                                                                                             <  1:565700/141‑1 (MQ=255)
    catcatTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAAt                                                                                                                                         <  1:7526/141‑1 (MQ=255)
      tcatTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGa                                                                                                                                       >  2:570315/1‑141 (MQ=255)
      tcatTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGa                                                                                                                                       >  2:570299/1‑141 (MQ=255)
       cactCCGATTGGATAAATCGCATTCGTCACTGATTCCATACTATGAGTCATCTTACGCTCTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGAt                                                                                                                                      <  1:285084/138‑1 (MQ=255)
        atTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATa                                                                                                                                     >  1:300059/1‑141 (MQ=255)
        atTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATa                                                                                                                                     >  1:597798/1‑141 (MQ=255)
           ccGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGAt                                                                                                                                  >  2:283476/1‑141 (MQ=255)
              aTTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCa                                                                                                                               <  1:711002/141‑1 (MQ=255)
                tGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCaaa                                                                                                                             <  2:71324/141‑1 (MQ=255)
                tGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCaaa                                                                                                                             <  1:519348/141‑1 (MQ=255)
                 ggATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAAt                                                                                                                            <  1:621522/141‑1 (MQ=255)
                      aaTCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGAtt                                                                                                                       >  2:491644/1‑141 (MQ=255)
                        tCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTg                                                                                                                     >  2:301652/1‑141 (MQ=255)
                                      gATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGc                                                                                                       <  1:570299/141‑1 (MQ=255)
                                      gATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGc                                                                                                       <  1:570315/141‑1 (MQ=255)
                                       aTTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATCTAAATGATAGATCCAAATAGATTTGTAGGTTTGTCATGcc                                                                                                      <  2:559493/141‑1 (MQ=255)
                                          ccATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAg                                                                                                   >  2:19806/1‑141 (MQ=255)
                                           cATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGt                                                                                                  <  2:62606/141‑1 (MQ=255)
                                                 atGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCACAGTTACAAg                                                                                            >  1:464091/1‑141 (MQ=255)
                                                 atGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCACAGTTACAAg                                                                                            >  1:378699/1‑141 (MQ=255)
                                                  tGATTCATCTTACGTTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCGTGCCCAGTTACAAGt                                                                                           <  1:704437/141‑1 (MQ=255)
                                                  tGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGt                                                                                           <  2:33314/141‑1 (MQ=255)
                                                  tGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGt                                                                                           <  2:605482/141‑1 (MQ=255)
                                                     ttCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGc                                                                                        >  1:426175/1‑141 (MQ=255)
                                                     ttCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGc                                                                                        <  2:85391/141‑1 (MQ=255)
                                                                 tttCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGac                                                                            <  2:669841/141‑1 (MQ=255)
                                                                     tcATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTa                                                                        >  2:616233/1‑141 (MQ=255)
                                                                      cATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTaa                                                                       >  1:168661/1‑141 (MQ=255)
                                                                        ttAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACa                                                                     >  1:43454/1‑141 (MQ=255)
                                                                        ttAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCAGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACa                                                                     <  2:687558/141‑1 (MQ=255)
                                                                          aaTCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGTTGTCCCTGGTGTTCCTATCATATATATGATAGATTCAAATTGATTTGTAGGTTTGTCATGCCGAGTTATAAGTTGCTTTATCGTAGACACTCACAtt                                                                   <  2:195461/141‑1 (MQ=255)
                                                                             cccATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTTCGTTATCGTAGACACTAACATTAat                                                                >  2:293216/1‑141 (MQ=255)
                                                                                 tCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGa                                                            <  2:225038/141‑1 (MQ=255)
                                                                                   gTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACt                                                          <  1:64707/141‑1 (MQ=255)
                                                                                      aCGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGt                                                       <  2:678638/141‑1 (MQ=255)
                                                                                             cAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGCTTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATCAATATGACTGGTAATGTTg                                                >  2:496358/1‑141 (MQ=255)
                                                                                               aataatACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCt                                              >  1:562918/1‑141 (MQ=255)
                                                                                                ataataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTg                                             <  2:433949/141‑1 (MQ=255)
                                                                                                 taataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGt                                            >  1:633882/1‑141 (MQ=255)
                                                                                                 taataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGt                                            >  1:463661/1‑141 (MQ=255)
                                                                                                 taataCTGCCATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGt                                            <  1:526208/141‑1 (MQ=255)
                                                                                                   ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                   <  1:563325/52‑2 (MQ=37)
                                                                                                   ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                   <  1:563281/52‑2 (MQ=37)
                                                                                                   ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                   >  2:563325/1‑51 (MQ=37)
                                                                                                   ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                   >  2:563281/1‑51 (MQ=37)
                                                                                                   ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGGTTTGTAGGTTTTTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTta                                          >  2:133581/1‑141 (MQ=255)
                                                                                                   ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTta                                          >  1:149158/1‑141 (MQ=255)
                                                                                                    taCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATACAAATGATAGATTCACCATGATTTGTCTCTTGTTCATGCCCATTTACAATTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTtaa                                         <  1:420124/141‑1 (MQ=255)
                                                                                                     aCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTtaat                                        >  2:403948/1‑141 (MQ=255)
                                                                                                         cTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAAc                                    >  1:191150/1‑141 (MQ=255)
                                                                                                         cTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAAc                                    >  1:191361/1‑141 (MQ=255)
                                                                                                         cTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAAc                                    >  1:234594/1‑141 (MQ=255)
                                                                                                                    gCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAAt                         >  1:526376/1‑141 (MQ=255)
                                                                                                                       gTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCt                      <  1:10624/141‑1 (MQ=255)
                                                                                                                       gTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCt                      <  1:10690/141‑1 (MQ=255)
                                                                                                                           ctgctgTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCa                  <  1:112083/141‑1 (MQ=255)
                                                                                                                             gctgTTCCTATCATATAAATGATAGATTCAAATTGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCAtc                <  2:537067/141‑1 (MQ=255)
                                                                                                                             gctgTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCAtc                <  2:279904/141‑1 (MQ=255)
                                                                                                                                      aTCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATcc       <  1:259610/141‑1 (MQ=255)
                                                                                                                                        cATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGt     <  1:416996/141‑1 (MQ=255)
                                                                                                                                        cATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGt     <  1:447942/141‑1 (MQ=255)
                                                                                                                                           ataAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTaaa  >  1:284131/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ATAACATCATTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTAAA  >  CP000730/2636823‑2637102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: