Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,680,166 T→C 10.9% intergenic (+218/+314) cbpD → / ← USA300HOU_2537 possible secretory antigen/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,680,1660TC10.9% 173.5 / 10.6 64intergenic (+218/+314)cbpD/USA300HOU_2537possible secretory antigen/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/35);  new base C (3/4);  total (25/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAA  >  CP000730/2680027‑2680307
                                                                                                                                           |                                                                                                                                                 
taCTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGAtata                                                                                                                                                  <  1:681520/141‑1 (MQ=255)
 tcTTCTACAAATTTCAAATTGCCCTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATaa                                                                                                                                                 <  2:643580/140‑1 (MQ=255)
  tttCTACAATTTTCAGATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATACTATTATTTAAATAATTCGCTATAACTTAACATGCGTTTTCGATATaaa                                                                                                                                                <  2:317542/140‑1 (MQ=255)
     cTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCt                                                                                                                                             <  1:165389/141‑1 (MQ=255)
     cTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCt                                                                                                                                             >  2:386494/1‑141 (MQ=255)
       aCAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTg                                                                                                                                           <  2:665491/141‑1 (MQ=255)
         aaaTTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGtt                                                                                                                                         <  1:604382/141‑1 (MQ=255)
          aaTTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAAAAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTc                                                                                                                                        >  1:180995/1‑141 (MQ=255)
                aaaTTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAAtc                                                                                                                                  <  1:679995/141‑1 (MQ=255)
                             tGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAAtttttt                                                                                                                  <  2:194153/141‑1 (MQ=255)
                                   tataTTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGtt                                                                                                           <  2:191955/141‑1 (MQ=255)
                                       tttCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCa                                                                                                       <  2:51644/141‑1 (MQ=255)
                                                 aaCTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTAGATTTATACCTTGTTCTATCTCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAg                                                                                             >  1:491093/1‑141 (MQ=255)
                                                   cTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGtt                                                                                           <  1:71232/141‑1 (MQ=255)
                                                    caaattaccATCTTCGGCATCGTCTAAGTTTAAAATTTTATGCTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACTAACCTTTTTCTAAATCACT‑‑TACAATTTCTTGATTTTTTTCGTCGTCATCAGt                                                                                            <  2:185798/133‑1 (MQ=255)
                                                      ttattTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTaaa                                                                                        >  2:425276/1‑141 (MQ=255)
                                                      ttattTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTaaa                                                                                        >  1:243904/1‑141 (MQ=255)
                                                         tttCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTaaaaaa                                                                                     <  1:662634/141‑1 (MQ=255)
                                                         tttCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTaaaaaa                                                                                     <  1:662637/141‑1 (MQ=255)
                                                         tttCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTaaaaaa                                                                                     <  1:662660/141‑1 (MQ=255)
                                                             aTCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAaataa                                                                                 >  1:474850/1‑141 (MQ=255)
                                                                  tAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTaa                                                                            >  1:662603/1‑141 (MQ=255)
                                                                   aGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAAc                                                                           >  2:427991/1‑141 (MQ=255)
                                                                   aGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAAc                                                                           >  2:295574/1‑141 (MQ=255)
                                                                    gCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACt                                                                          <  1:401871/141‑1 (MQ=255)
                                                                       tAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAATTGAg                                                                       >  1:394452/1‑141 (MQ=255)
                                                                       tAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAg                                                                       <  1:583869/141‑1 (MQ=255)
                                                                       tAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAg                                                                       >  1:525950/1‑141 (MQ=255)
                                                                       tAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAg                                                                       <  1:295574/141‑1 (MQ=255)
                                                                         aTCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGtt                                                                     >  1:87450/1‑141 (MQ=255)
                                                                           ctaagtttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAAcc                                                                                                                                              <  1:6147/63‑1 (MQ=255)
                                                                           ctaagtttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAAcc                                                                                                                                              >  2:6147/8‑70 (MQ=255)
                                                                              agtttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAAtcactt                                                                                                                              >  1:523125/5‑79 (MQ=255)
                                                                              agtttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAAtcactt                                                                                                                              <  2:523125/79‑5 (MQ=255)
                                                                               gtttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAAtcactta                                                                                                                             >  2:669334/4‑78 (MQ=255)
                                                                               gtttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAAtcactta                                                                                                                             <  1:669334/80‑6 (MQ=255)
                                                                                 ataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGAc                                                             <  1:126136/141‑1 (MQ=255)
                                                                                    aaaTTTTATGCTATTATTTAAATAATTAGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTT‑‑‑AATAATTTTTTTCTGTTTTCATAGTAATAAGATAAAAAAATAATTTAACTGAGTTTTTGAGACCTg                                                          <  2:383740/141‑1 (MQ=255)
                                                                                     aaTTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGAcc                                                            <  1:154507/141‑1 (MQ=255)
                                                                                        tttATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTa                                                      <  2:708587/141‑1 (MQ=255)
                                                                                         ttATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGAAAAAAAAAAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTaa                                                     <  2:512610/141‑1 (MQ=255)
                                                                                           aTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAAt                                                   >  2:381549/1‑141 (MQ=255)
                                                                                           aTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAANTTAACTGAGTTTNTGAGACCTGTTTAAAt                                                   <  1:394475/141‑1 (MQ=255)
                                                                                            tGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAAtt                                                  >  1:610512/1‑141 (MQ=255)
                                                                                                attattTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATtgatg                                              <  1:640241/141‑1 (MQ=255)
                                                                                                attattTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATtgatg                                              <  1:599351/141‑1 (MQ=255)
                                                                                                  tattTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGAc                                            >  1:103019/1‑141 (MQ=255)
                                                                                                   attTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACt                                           >  1:658330/1‑141 (MQ=255)
                                                                                                    tttAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTc                                          <  2:369981/141‑1 (MQ=255)
                                                                                                       aaaTAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTTAATCTA‑‑‑AAAACATGTTTGCTTGTTTTATTTACATTATGTAAAATAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCaaa                                       >  1:137004/1‑141 (MQ=255)
                                                                                                          taatTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGAAAAAAAATAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAAcc                                    <  2:118726/141‑1 (MQ=255)
                                                                                                               cGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTAt                               >  1:657242/1‑141 (MQ=255)
                                                                                                                       cTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCtttt                       <  1:53324/141‑1 (MQ=255)
                                                                                                                       cTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCtttt                       >  1:680348/1‑141 (MQ=255)
                                                                                                                        ttAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTc                      <  2:15227/141‑1 (MQ=255)
                                                                                                                          aaCATACGCTTTCGATATAATCCTTGTGCTACATCAC‑‑‑AATACTTTTTAGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGATTTTGAGACCTGTTTAAATTAATGACTCAAAACCTTTATACCCTTTTCAc                    >  2:438827/1‑141 (MQ=255)
                                                                                                                               aCGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGt               <  2:491093/141‑1 (MQ=255)
                                                                                                                               aCGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGt               <  1:455922/141‑1 (MQ=255)
                                                                                                                                cGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGtt                                                                                                           <  2:503510/48‑1 (MQ=255)
                                                                                                                                cGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGtt                                                                                                           >  1:503510/1‑48 (MQ=255)
                                                                                                                                cGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGtt              <  1:225116/141‑1 (MQ=255)
                                                                                                                                    ttCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTAGCCCCTTTTCACTCGGTTTAAt          <  2:268760/141‑1 (MQ=255)
                                                                                                                                     tCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAAtt         <  2:662603/141‑1 (MQ=255)
                                                                                                                                      cGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTg        <  2:78980/141‑1 (MQ=255)
                                                                                                                                       gATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTGTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGc       >  1:18008/1‑141 (MQ=255)
                                                                                                                                          ataAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTTTTTTTTTCTTCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCttt    >  2:612709/1‑141 (MQ=255)
                                                                                                                                           taAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTTCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTaa  >  2:498893/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
TACTTCTACAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTC‑‑‑AATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAA  >  CP000730/2680027‑2680307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: