Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,787,555 G→A 5.2% intergenic (+101/‑139) USA300HOU_2639 → / → USA300HOU_2640 hypothetical protein/BglG family transcriptional antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,787,5550GA 5.2% 235.9 / 2.9 77intergenic (+101/‑139)USA300HOU_2639/USA300HOU_2640hypothetical protein/BglG family transcriptional antiterminator
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base A (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

TCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGTTAAA  >  CP000730/2787416‑2787694
                                                                                                                                           |                                                                                                                                           
tCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGc                                                                                                                                            >  1:558037/1‑141 (MQ=255)
     acaaacaCCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAAt                                                                                                                                       <  1:111192/141‑1 (MQ=255)
       aaacaCCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTc                                                                                                                                     <  2:8390/141‑1 (MQ=255)
        gacacCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTTTGTAAGTATCAGATGCATAAATTCAGAAAAACCGCAGTGAGATCTTTTCCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCg                                                                                                                                    <  2:114209/140‑1 (MQ=255)
        aacaCCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCg                                                                                                                                    >  2:176292/1‑141 (MQ=255)
           acCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCAc                                                                                                                                 <  1:430178/141‑1 (MQ=255)
           acCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCAc                                                                                                                                 >  1:173423/1‑141 (MQ=255)
             cTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCActc                                                                                                                               >  2:431433/1‑141 (MQ=255)
             cTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTCCTTTATTTGCATAATTCGCActc                                                                                                                               >  1:265388/1‑141 (MQ=255)
              tCAAGCACATTCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCATTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCActct                                                                                                                              <  1:299188/141‑1 (MQ=255)
              tCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAACACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCActct                                                                                                                              <  2:691833/141‑1 (MQ=255)
              tCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCActct                                                                                                                              <  2:661716/141‑1 (MQ=255)
                aaGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGt                                                                                                                            >  2:251393/1‑141 (MQ=255)
                   cacaATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTaa                                                                                                                         >  2:221262/1‑141 (MQ=255)
                     caATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTaaaa                                                                                                                       <  1:567586/141‑1 (MQ=255)
                      aaTCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGACAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAAt                                                                                                                      <  1:281290/141‑1 (MQ=255)
                      aaTCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATTTTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTACATAATTCGCACTCTGTTAAAAt                                                                                                                      >  2:164061/1‑141 (MQ=255)
                      aaTCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATTTTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTACATAATTCGCACTCTGTTAAAAt                                                                                                                      >  2:164135/1‑141 (MQ=255)
                       aTCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATc                                                                                                                     >  1:165906/1‑141 (MQ=255)
                       aTCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTCCTATTTGAAATATCTTACTTCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATc                                                                                                                     >  2:613876/1‑141 (MQ=255)
                         cACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCat                                                                                                                   >  1:124728/1‑141 (MQ=255)
                           ccTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCatat                                                                                                                 <  1:675/141‑1 (MQ=255)
                           ccTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCatat                                                                                                                 >  2:22746/1‑141 (MQ=255)
                                  aTTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTCTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGtt                                                                                                          <  1:587115/141‑1 (MQ=255)
                                        cATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCttt                                                                                                    >  1:513044/1‑141 (MQ=255)
                                        cATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCttt                                                                                                    <  2:638697/141‑1 (MQ=255)
                                              ccTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTc                                                                                              >  1:391282/1‑141 (MQ=255)
                                                  aTACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGGGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTg                                                                                          >  2:242667/1‑141 (MQ=255)
                                                   tACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGa                                                                                         >  1:710304/1‑141 (MQ=255)
                                                         cacaAGTACCAGATGCATAAATTCAGAAAAACATCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAACAGTTCGCTTTAATGTCATTGACgtatg                                                                                   <  2:19506/141‑1 (MQ=255)
                                                           caAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTGTATTTTAGATATCTGACTGCTGTTCTTTATTGGCATGATTCGCACTCTGTTAAAATCATATAGAAGTGCGCTTTTATGTCATGGACgtaggga                                                                                 >  2:242195/1‑137 (MQ=255)
                                                           caAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACgtatgta                                                                                 <  1:159018/141‑1 (MQ=255)
                                                              gTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATaa                                                                              >  2:492168/1‑141 (MQ=255)
                                                                     aTGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCa                                                                       <  1:159402/141‑1 (MQ=255)
                                                                        cATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAACAGTGCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGt                                                                    <  2:415635/141‑1 (MQ=255)
                                                                          tAAATTCAGAAAAACAGCAGTGAGATATTTTCCTATTTGAAATATCTTACTTCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGttt                                                                  <  1:613876/141‑1 (MQ=255)
                                                                            aaTTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTtaa                                                                <  1:176292/141‑1 (MQ=255)
                                                                             aTTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTtaat                                                               >  1:131642/1‑141 (MQ=255)
                                                                                 aGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAataata                                                           <  2:30446/141‑1 (MQ=255)
                                                                                 aGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAataata                                                           >  2:382385/1‑141 (MQ=255)
                                                                                  gAAAAACAGCAGTGAGATATTTTTTTTTTTTTTTTATCTTACTGCTGTTCTTTATTTTAATAATTCGCACTCTGTTAAAATCATATATAAGTTCGCTTTAATTTCATTGACAAATGTATAAGCTCTCATGTTTTATTatat                                                          >  2:680424/1‑141 (MQ=255)
                                                                                  gAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAatat                                                          >  2:556462/1‑141 (MQ=255)
                                                                                  gAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAatat                                                          >  1:427147/1‑141 (MQ=255)
                                                                                     aaaCAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTaa                                                       <  1:598012/141‑1 (MQ=255)
                                                                                       aCAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATa                                                     >  1:146762/1‑141 (MQ=255)
                                                                                         agcatTCAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCTCACTCTGTTAAAATCATATATAAGTTCGCTTTAATGGCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATaca                                                   <  2:4420/141‑1 (MQ=255)
                                                                                           cagTGAGATATTTTTTTTTTTTTTATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACAccc                                                >  2:660227/1‑141 (MQ=255)
                                                                                           cagTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACAcc                                                 <  1:121941/141‑1 (MQ=255)
                                                                                                 ggtatTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTGAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTAGAAGCTCTCATGTTTAATAATATTAATACACCCTTaaa                                           >  2:586398/3‑141 (MQ=255)
                                                                                                 gaTATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATNTTAATACACCCTTaaa                                           <  1:407290/141‑1 (MQ=255)
                                                                                                  atatTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAACCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTaaaa                                          >  2:302883/1‑141 (MQ=255)
                                                                                                          cTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTac                                  <  2:185292/141‑1 (MQ=255)
                                                                                                           tATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCTCTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTaca                                 <  1:585532/141‑1 (MQ=255)
                                                                                                            aTTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTacac                                <  1:240700/141‑1 (MQ=255)
                                                                                                             tttGAAATATCTTACTGCTGTTCTTTATTTACATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACg                               <  1:164061/141‑1 (MQ=255)
                                                                                                             tttGAAATATCTTACTGCTGTTCTTTATTTACATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACg                               <  1:164135/141‑1 (MQ=255)
                                                                                                               ttttatatCTTACTGCAGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATTTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGaa                             >  2:692536/5‑141 (MQ=255)
                                                                                                                gAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGaaa                            >  2:611678/1‑141 (MQ=255)
                                                                                                                gAAATATATTACAGCTGTTCTTTATTTGCATAATTAGAAATCTGTTAAAATCATATAAAAGTTCGCTTTAATGCCATTGACGTATGTATAAACTCTCATGTTTAATAATATTAAAAAAAAAAAAAAAACGCTTACACGaaa                            <  2:140964/141‑1 (MQ=255)
                                                                                                                    tatCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAAtt                        <  2:5995/141‑1 (MQ=255)
                                                                                                                    tatCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGATAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAAAGCTAAAAAAAAAAAtt                        <  2:558037/141‑1 (MQ=255)
                                                                                                                     atCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAAttt                       <  2:226508/141‑1 (MQ=255)
                                                                                                                      tCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTa                      >  2:183857/1‑141 (MQ=255)
                                                                                                                      tCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTa                      >  2:280743/1‑141 (MQ=255)
                                                                                                                      tCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTa                      >  1:282981/1‑141 (MQ=255)
                                                                                                                       cTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAg                     <  2:411418/141‑1 (MQ=255)
                                                                                                                       cTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAg                     >  1:312478/1‑141 (MQ=255)
                                                                                                                            tgctgTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAagg                <  1:131909/141‑1 (MQ=255)
                                                                                                                                  tCTTTATTTGCATAATTCGCACTCTGTTAAAAACATATAAAAGCTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCAAATTTAATAATATTAATACACCCATAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTAc          <  2:343564/141‑1 (MQ=255)
                                                                                                                                    tttATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACAt        <  1:677025/141‑1 (MQ=255)
                                                                                                                                     ttATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATg       >  2:545542/1‑141 (MQ=255)
                                                                                                                                     ttATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATg       >  1:647622/1‑141 (MQ=255)
                                                                                                                                      tATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATCAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGt      <  1:674221/141‑1 (MQ=255)
                                                                                                                                      tATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGt      <  1:674288/141‑1 (MQ=255)
                                                                                                                                       aTTTGCATAATTCGCACTCTGTTAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGTTa    <  1:660227/141‑1 (MQ=255)
                                                                                                                                        tttGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGTTa    <  2:381189/141‑1 (MQ=255)
                                                                                                                                          tGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGTTaaa  >  1:671391/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCATGTTTAATAATATTAATACACCCTTAAAAACGCTTACACGAAAAATTTAGAAAGGAGGTACATGTTAAA  >  CP000730/2787416‑2787694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: