Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 79,612 A→T 5.7% intergenic (+221/‑39) USA300HOU_0076 → / → USA300HOU_0077 transposase/possible S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073079,6120AT 5.7% 280.8 / 6.6 88intergenic (+221/‑39)USA300HOU_0076/USA300HOU_0077transposase/possible S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (40/43);  new base T (2/3);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

ATTATAAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATAAAAATGC  >  CP000730/79472‑79750
                                                                                                                                            |                                                                                                                                          
aTTATAAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCaaaa                                                                                                                                            >  1:358595/1‑141 (MQ=255)
aTTATAAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTGTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCaaaa                                                                                                                                            >  2:149499/1‑141 (MQ=255)
 ttATAAGTACTTGACCAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAAc                                                                                                                                           >  2:124983/1‑141 (MQ=255)
  tataAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACg                                                                                                                                          <  2:459356/141‑1 (MQ=255)
  tataAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCAAGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACg                                                                                                                                          <  2:550663/141‑1 (MQ=255)
   ataAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGt                                                                                                                                         <  2:342926/141‑1 (MQ=255)
   ataAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGt                                                                                                                                         <  2:298032/141‑1 (MQ=255)
      aGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAATCGTAat                                                                                                                                      >  2:119862/1‑141 (MQ=255)
          cTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAattatt                                                                                                                                  <  2:21794/141‑1 (MQ=255)
          cTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAattatt                                                                                                                                  <  2:211426/141‑1 (MQ=255)
           ttGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAATCGTAAtaatta                                                                                                                                 <  1:119862/141‑1 (MQ=255)
           ttGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAAttatta                                                                                                                                 <  1:481324/141‑1 (MQ=255)
                tAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAATCGTAATAATTACgttt                                                                                                                            <  1:51473/141‑4 (MQ=255)
                tAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGAtt                                                                                                                            <  1:167196/141‑1 (MQ=255)
                   tCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGa                                                                                                                         <  1:159485/141‑1 (MQ=255)
                    cGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAATCGTAATAATTACGTTTTGaa                                                                                                                        <  2:355371/141‑1 (MQ=255)
                    cGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGaa                                                                                                                        <  1:35803/141‑1 (MQ=255)
                    cGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCAAGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGaa                                                                                                                        <  2:429364/141‑1 (MQ=255)
                        agaaagaGCCTAGGACATATATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTACTCAAAACGTAATTATTACGACTTGAATTaa                                                                                                                    <  2:603551/141‑1 (MQ=255)
                         gaaagaGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAg                                                                                                                   >  1:39104/1‑141 (MQ=255)
                         gaaagaGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAg                                                                                                                   >  1:97802/1‑141 (MQ=255)
                              agCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTCATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACAGAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCgagg                                                                                                              >  1:107517/1‑141 (MQ=255)
                              agCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCgagg                                                                                                              <  2:426032/141‑1 (MQ=255)
                               gCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCgagga                                                                                                             >  2:468548/1‑141 (MQ=255)
                               gCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCgagga                                                                                                             >  1:102932/1‑141 (MQ=255)
                                  tAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGaa                                                                                                          >  1:100195/1‑141 (MQ=255)
                                  tAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGaa                                                                                                          >  1:439822/1‑141 (MQ=255)
                                     gACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGaatga                                                                                                       <  2:136262/141‑1 (MQ=255)
                                       cATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGaatgaaa                                                                                                     <  2:121718/141‑1 (MQ=255)
                                         tAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAat                                                                                                                                      <  2:325832/106‑1 (MQ=255)
                                         tAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAat                                                                                                                                      >  1:325832/1‑106 (MQ=255)
                                          aaaTCAATGTCTCGCGACCACATCTTAATTTTTGGTGTTCATTCATGACTTGAAAGAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAAGTATGACGATTTGGATTAAGCTAGGAGaatgaaatga                                                                                                  <  2:528893/141‑1 (MQ=255)
                                          aaaTCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGaatgaaatga                                                                                                  <  2:459025/141‑1 (MQ=255)
                                           aaTCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGAc                                                                                                 >  1:640627/1‑141 (MQ=255)
                                            aTCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAatta                                                                                                                                    >  2:286946/1‑105 (MQ=255)
                                            aTCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAatta                                                                                                                                    <  1:286946/105‑1 (MQ=255)
                                                aTGTCTCGCGACCACAGCTTCATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGa                                                                                            <  2:211851/141‑1 (MQ=255)
                                                  gTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACt                                                                                          <  2:358595/141‑1 (MQ=255)
                                                   tctcGCGACCACAGCTTAATTTTTTGTGTTTATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACtt                                                                                         >  2:488058/1‑141 (MQ=255)
                                                    ctcGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTa                                                                                        >  1:307986/1‑141 (MQ=255)
                                                     tcGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTAt                                                                                       >  2:483899/1‑141 (MQ=255)
                                                     tcGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAACGAGGAGAATGAAATGAATAAGACTTaa                                                                                       >  1:218476/1‑140 (MQ=255)
                                                        cgACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGAc                                                                                    <  1:386344/141‑1 (MQ=255)
                                                        cgACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGAc                                                                                    <  2:109548/141‑1 (MQ=255)
                                                        cgACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGAc                                                                                    >  1:693672/1‑141 (MQ=255)
                                                              caGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTtgg                                                                              >  1:233586/1‑141 (MQ=255)
                                                                   tAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCa                                                                         <  2:336891/141‑1 (MQ=255)
                                                                          tGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGt                                                                  >  1:10303/1‑141 (MQ=255)
                                                                          tGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGt                                                                  <  1:427391/141‑1 (MQ=255)
                                                                                  attcatGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATc                                                          <  1:255793/141‑1 (MQ=255)
                                                                                      atGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgat                                                      >  1:251994/1‑141 (MQ=255)
                                                                                       tGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatg                                                     >  2:387603/1‑141 (MQ=255)
                                                                                       tGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatg                                                     >  2:78782/1‑141 (MQ=255)
                                                                                        gACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatga                                                    >  1:280466/1‑141 (MQ=255)
                                                                                         aCTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatgat                                                   <  1:428409/141‑1 (MQ=255)
                                                                                          cTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatgatg                                                  >  2:321154/1‑141 (MQ=255)
                                                                                           tttAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatgatga                                                 >  2:31304/1‑141 (MQ=255)
                                                                                            ttAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatgatgat                                                <  2:583927/141‑1 (MQ=255)
                                                                                            ttAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatgatgat                                                <  2:400455/141‑1 (MQ=255)
                                                                                               aaaaaTCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATg                                             <  1:88958/141‑1 (MQ=255)
                                                                                                 aaaTCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGc                                           >  1:324542/1‑141 (MQ=255)
                                                                                                 aaaTCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGc                                           >  2:583084/1‑141 (MQ=255)
                                                                                                  aaTCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCa                                          >  1:193929/1‑141 (MQ=255)
                                                                                                   atcgtacgtGCATATATGGACAAGGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAGTGATATGGCTAATGCTGATGAAGAAGGAAAAAAAAAAGGTCAAGAATCAGATGATGATATGGCAc                                         <  2:139634/133‑1 (MQ=255)
                                                                                                    tCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACg                                        >  2:662484/1‑141 (MQ=255)
                                                                                                    tCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACg                                        >  1:641281/1‑141 (MQ=255)
                                                                                                    tCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACg                                        >  1:694478/1‑141 (MQ=255)
                                                                                                    tCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACg                                        >  2:705203/1‑141 (MQ=255)
                                                                                                    tCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACg                                        >  1:246752/1‑141 (MQ=255)
                                                                                                        tATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAgatgatgatggtctcgtgcca                                    <  1:213954/141‑13 (MQ=255)
                                                                                                         aTTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTGCGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGAcc                                   >  1:577842/1‑141 (MQ=255)
                                                                                                         aTTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTCCGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCACAAAGGTCACGAATCAGAGGATGATATGGCACGAGAcc                                   >  1:99299/1‑141 (MQ=255)
                                                                                                         aTTGCATAAACGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCAGGAGAcc                                   >  2:379558/1‑141 (MQ=255)
                                                                                                          ttGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCa                                  >  2:175649/1‑141 (MQ=255)
                                                                                                             cATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCa                               <  1:521075/141‑1 (MQ=255)
                                                                                                              aTAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCaa                              <  2:228710/141‑1 (MQ=255)
                                                                                                              aTAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTCTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCaa                              <  2:580854/141‑1 (MQ=255)
                                                                                                                aaaTGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCaaga                            >  1:468413/1‑141 (MQ=255)
                                                                                                                aaaTGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACAAAAAAAAAAAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCaaga                            <  2:442041/141‑1 (MQ=255)
                                                                                                                    gTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCt                        >  1:24376/1‑141 (MQ=255)
                                                                                                                     tACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCtt                       <  2:307986/141‑1 (MQ=255)
                                                                                                                       cATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTgg                     <  2:233586/141‑1 (MQ=255)
                                                                                                                        aTAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTggg                    <  1:215334/141‑1 (MQ=255)
                                                                                                                               agtaCTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAAc             <  2:96512/141‑1 (MQ=255)
                                                                                                                                gtaCTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAGTCAGATGATGATATGGCACGAGATCATCAAGAAGCTTGGGAGAGAACa            >  2:689462/1‑141 (MQ=255)
                                                                                                                                  aCTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAAt          <  2:193929/141‑1 (MQ=255)
                                                                                                                                  aCTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAAt          >  1:61886/1‑141 (MQ=255)
                                                                                                                                   cTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATa         <  2:24376/141‑1 (MQ=255)
                                                                                                                                   cTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATa         <  1:658995/141‑1 (MQ=255)
                                                                                                                                        cAAATCGTAATAATTACGTTTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATAAAAAt    >  2:429751/1‑141 (MQ=255)
                                                                                                                                          aaaCGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATAAAAATGc  >  1:186318/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
ATTATAAGTACTTGACTAATCGTAAGAAAGAGCCTAGGACATAAATCAATGTCTCGCGACCACAGCTTAATTTTTGGTGTTCATTCATGACTTTAAAAAATCCTTATTGCATAAATGTACATAGTGTAGTACTATTCAAAACGTAATTATTACGATTTGAATTAAGCGAGGAGAATGAAATGACTAAGACTTATGACGTTTGGTGGCAAAAAGGTCAAGAATCAGATGATGATATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATAAAAATGC  >  CP000730/79472‑79750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: