Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	111,516	C→A	31.8%	Q196K (CAA→AAA)	USA300HOU_0110 →	possible staphylococcal tandem lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	111,516	0	C	A	31.8%	160.4 / 80.2	91	Q196K (CAA→AAA)	USA300HOU_0110	possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base A (11/18);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

AGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAA  >  CP000730/111379‑111656                                                                                                                                          |                                                                                                                                              aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                            <  1:648990/141‑1 (MQ=255) aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                            <  1:549940/141‑1 (MQ=255)  ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                           >  1:679472/1‑141 (MQ=255)    aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATc                                                                                                                                         >  1:624459/1‑141 (MQ=255)        tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAtt                                                                                                                                     <  1:327539/141‑1 (MQ=255)         aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                                    >  2:35040/1‑141 (MQ=255)          aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                                   >  1:9522/1‑141 (MQ=255)          aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                                   <  1:399088/141‑1 (MQ=255)            gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                                                                 <  1:220190/141‑1 (MQ=255)              agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaa                                                                                                                               >  2:199344/1‑141 (MQ=255)              agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                                                               <  2:661506/141‑1 (MQ=255)               gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                              >  2:358080/1‑141 (MQ=255)                aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                                                             <  2:240721/141‑1 (MQ=255)                aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatg                                                                                                                             <  1:85562/141‑1 (MQ=255)                 aaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                                                                            <  1:478230/141‑1 (MQ=255)                  aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                           >  1:83673/1‑141 (MQ=255)                  aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                           <  1:560453/141‑1 (MQ=255)                  aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                           <  1:298643/141‑1 (MQ=255)                  aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                           >  2:667572/1‑141 (MQ=255)                   aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                          >  1:80596/1‑141 (MQ=255)                    cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                         <  2:176883/141‑1 (MQ=255)                      tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGTGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                       >  2:400399/1‑141 (MQ=255)                       aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                                      <  1:487595/141‑1 (MQ=255)                        aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGTAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAATTAACTAATGATGATtat                                                                                                                     >  2:518099/1‑141 (MQ=255)                        aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                     >  1:467530/1‑141 (MQ=255)                        aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                     <  1:209729/141‑1 (MQ=255)                        aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                                     <  2:548208/141‑1 (MQ=255)                         aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTCAATATCAATTAACTAATGATGATtata                                                                                                                    >  2:337342/1‑141 (MQ=255)                             aGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGt                                                                                                                >  2:208877/1‑141 (MQ=255)                             aGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGt                                                                                                                <  2:60301/141‑1 (MQ=255)                               aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑aa                                                                                                             <  1:214658/141‑1 (MQ=255)                               aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑aa                                                                                                             <  2:440747/141‑1 (MQ=255)                               aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑aa                                                                                                             <  1:223959/141‑1 (MQ=255)                                aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑aaa                                                                                                            >  1:450649/1‑141 (MQ=255)                                   cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACa                                                                                                         >  1:560540/1‑141 (MQ=255)                                        aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAc                                                                                                    <  2:74266/141‑1 (MQ=255)                                        aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAc                                                                                                    >  1:475455/1‑141 (MQ=255)                                          tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACgc                                                                                                  <  1:550790/141‑2 (MQ=255)                                              aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCaaaa                                                                                              <  1:342344/141‑1 (MQ=255)                                              aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTaaaa                                                                                              >  2:461140/1‑141 (MQ=255)                                                  ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGata                                                                                          <  1:667751/141‑1 (MQ=255)                                                     tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGa                                                                                       >  1:595766/1‑141 (MQ=255)                                                     tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGa                                                                                       <  2:613170/141‑1 (MQ=255)                                                     tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGa                                                                                       >  1:92312/1‑141 (MQ=255)                                                        gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAgatatgatat                                                                                    >  2:164281/1‑141 (MQ=255)                                                        gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGatat                                                                                    <  2:544542/141‑4 (MQ=14)                                                         ttttttttcAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGTCTATATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGAtata                                                                                   >  2:35358/9‑141 (MQ=11)                                                         cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATa                                                                                   <  2:411197/141‑1 (MQ=255)                                                             ataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGATATAccga                                                                               <  1:508147/141‑3 (MQ=14)                                                               aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATGATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGATATAccgacg                                                                             <  2:453738/141‑5 (MQ=11)                                                                gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACcaacaa                                                                            >  1:699214/1‑141 (MQ=255)                                                                 cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACgc                                                                                                  >  1:44390/1‑117 (MQ=255)                                                                 cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACgc                                                                                                  <  2:44390/118‑2 (MQ=255)                                                                    ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                        >  2:85998/1‑141 (MQ=255)                                                                     tttAAAAATTTAAAGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATTATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                       <  2:457341/141‑1 (MQ=255)                                                                       tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                     <  1:220657/141‑1 (MQ=255)                                                                         aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                   >  1:215956/1‑141 (MQ=255)                                                                          aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                  <  1:440127/141‑1 (MQ=255)                                                                            aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGATTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                <  2:414608/141‑1 (MQ=255)                                                                              ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                              <  2:527341/141‑1 (MQ=255)                                                                                 aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                           <  1:337316/141‑1 (MQ=255)                                                                                  tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                          <  1:263745/141‑1 (MQ=255)                                                                                     aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                       >  1:177298/1‑141 (MQ=255)                                                                                        ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                    <  2:358477/141‑1 (MQ=255)                                                                                        ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                    >  1:464596/1‑141 (MQ=255)                                                                                         taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                    >  1:458472/1‑141 (MQ=255)                                                                                            aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                <  2:320763/141‑1 (MQ=255)                                                                                               tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                             >  1:630916/1‑141 (MQ=255)                                                                                                  agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                          >  1:294124/1‑141 (MQ=255)                                                                                                   gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGa                                                                                            >  2:653197/1‑90 (MQ=255)                                                                                                   gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGa                                                                                            <  1:653197/90‑1 (MQ=255)                                                                                                   gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                         <  2:402328/141‑1 (MQ=255)                                                                                                               aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                             <  1:506804/141‑1 (MQ=255)                                                                                                                  ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                          >  2:37798/1‑141 (MQ=255)                                                                                                                  ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGACGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAATGTACAGGGAATTTAAAAGGTtca                          >  2:92468/1‑141 (MQ=255)                                                                                                                    agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                  >  1:148937/1‑99 (MQ=255)                                                                                                                    agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                  <  2:148937/99‑1 (MQ=255)                                                                                                                    agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                        >  1:567262/1‑141 (MQ=255)                                                                                                                       ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                     <  2:245191/141‑1 (MQ=255)                                                                                                                          gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                  >  1:157139/1‑141 (MQ=255)                                                                                                                          gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                  >  1:296712/1‑141 (MQ=255)                                                                                                                          gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAATTAAAAGGTTCATCAGTTgg                  >  2:440830/1‑141 (MQ=255)                                                                                                                            cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                >  2:74488/1‑141 (MQ=255)                                                                                                                             gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata               <  2:616663/141‑1 (MQ=255)                                                                                                                             gAGTTATTCAGCTCAATATCAATTAACTAATGAGGATTATAATGT‑AAAACAATTACGTAAAATATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata               >  2:417917/1‑141 (MQ=255)                                                                                                                               gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata             >  1:469563/1‑141 (MQ=255)                                                                                                                                 tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAAt                                                                                                       >  2:535228/1‑49 (MQ=255)                                                                                                                                 tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAAt                                                                                                       <  1:535228/49‑1 (MQ=255)                                                                                                                                 tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa           <  1:21109/141‑1 (MQ=255)                                                                                                                                    tCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa        >  2:620957/1‑141 (MQ=255)                                                                                                                                         tCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa   >  1:452297/1‑141 (MQ=255)                                                                                                                                          cAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGaa  <  2:177298/141‑1 (MQ=255)                                                                                                                                          cAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGaa  <  2:445423/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                              AGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAA  >  CP000730/111379‑111656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑