Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 217,036 T→A 9.9% intergenic (‑203/+23) argD ← / ← brnQ ornithine‑‑oxo‑acid transaminase/possible LIVCS family branched chain amino acid:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730217,0360TA 9.9% 180.7 / 12.1 61intergenic (‑203/+23)argD/brnQornithine‑‑oxo‑acid transaminase/possible LIVCS family branched chain amino acid:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base A (3/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

ATAAAGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATATTTTTGG  >  CP000730/216899‑217176
                                                                                                                                         |                                                                                                                                            
ataAAGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAAc                                                                                                                                           >  1:389190/1‑141 (MQ=255)
  aaaGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACat                                                                                                                                         <  1:509883/141‑1 (MQ=255)
       tgaGGTCGTGTTTGTATTTATGTGAGTTGTAAAATGAAAGTACTAATATTTTTTGTGCATAATCATTGATACGTATGTATACGATTGCAGTTTAATGTACTATGAATCAAAAATGCCACATGCATATGTTTAACatatccc                                                                                                                                    <  2:96642/139‑4 (MQ=255)
       tAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGAc                                                                                                                                    <  1:576573/141‑1 (MQ=255)
       tAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGAc                                                                                                                                    >  2:292956/1‑141 (MQ=255)
         aGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACtg                                                                                                                                  <  1:486518/141‑1 (MQ=255)
          ggTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACtgt                                                                                                                                 >  2:108339/1‑141 (MQ=255)
            ttGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAACATGCCACAAGCATATGTTTAACATATGACTGTgg                                                                                                                               <  2:30969/141‑1 (MQ=255)
            ttGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAGTCATATGTTAAACATATgcttgtgg                                                                                                                               <  1:263009/141‑8 (MQ=39)
            ttGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAGTCATATGTTAAACATATgcttgtgg                                                                                                                               <  2:567322/141‑8 (MQ=39)
             tgtgTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGc                                                                                                                              <  1:410441/141‑1 (MQ=255)
              gtgtTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAGTCATATGTTAAACATATGCTTGTGGCa                                                                                                                             <  1:675278/141‑1 (MQ=38)
               tgtTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTTTTTTTTTTTATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCAt                                                                                                                            >  2:573732/1‑141 (MQ=255)
                   tGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGta                                                                                                                        >  1:651789/1‑141 (MQ=255)
                            gtgATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTtg                                                                                                               >  1:336746/1‑141 (MQ=255)
                               aTTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTgg                                                                                                            >  1:127126/1‑141 (MQ=255)
                                tttGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGa                                                                                                           >  1:145136/1‑141 (MQ=255)
                                        aTGAAAGTACTAATATTTTTTGTGTATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACg                                                                                                   >  2:250556/1‑141 (MQ=255)
                                        aTGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACg                                                                                                   >  2:593857/1‑141 (MQ=255)
                                          gAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGAt                                                                                                 <  2:234421/141‑1 (MQ=255)
                                            aaGTACTAATATTTTTTGTGGATAATCATTAATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGc                                                                                               <  2:398411/141‑1 (MQ=255)
                                                         ttttGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTataaa                                                                                  <  2:6505/141‑1 (MQ=255)
                                                           tttttttttttttttGATATGTATGTATAAGATTGTAGTTTAATGTAGTATGAATAAAAAATGCTACAAGTATATGTTTAAAATATTTTTTTTGTTTATTATTATGTTTGTGGAATTTTAAGATGTTTAATTTTataaata                                                                                >  2:685890/14‑141 (MQ=255)
                                                           ttGTGGATAATCATTGATATGTATGTATACGATTGCAGCTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTCCGATGCTTAATTTTataaata                                                                                >  1:466390/1‑141 (MQ=255)
                                                           ttGTGGATAATCATTGATATGTATGTATACGATTGAAGTTTAATGTAGTATGAATAAAAAATGAAACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATACTTGTGGAATTTTACCATGCTTAATTTTataaata                                                                                >  2:644147/1‑141 (MQ=255)
                                                            tgtgGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTataaataa                                                                               <  1:114886/141‑1 (MQ=255)
                                                            tgtgGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTataaataa                                                                               <  1:115000/141‑1 (MQ=255)
                                                             ttttttttttaTTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAaataat                                                                              >  2:30019/11‑141 (MQ=255)
                                                             gtgGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGAATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAaataat                                                                              >  1:586226/1‑141 (MQ=255)
                                                               ggATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGa                                                                            >  1:513251/1‑141 (MQ=255)
                                                                   aaTCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGcc                                                                        <  1:30610/141‑1 (MQ=255)
                                                                   aaTCATTGATATGTATGTATACCTATGCAGTTTAATGTAGTATGAATAAAAAATGCCACGAGCATATGTTTAACATATGACTGTGGAATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATACATAATGAAGcc                                                                        <  2:323568/141‑1 (MQ=255)
                                                                      taTTGATATGTATGCATACGATTGCAGTTTAATGTAGTATTAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATAATGCTTGTAGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGAt                                                                     <  2:261979/140‑1 (MQ=255)
                                                                       aTTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATa                                                                    >  2:99837/1‑141 (MQ=255)
                                                                             atgtatgtATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGaaa                                                              <  2:651789/141‑1 (MQ=255)
                                                                                tatgtatACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCa                                                           >  2:340359/1‑141 (MQ=255)
                                                                                 atgtatACGATTGCAGTTTAATAAAGAATAAAAAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCaa                                                          <  2:336250/141‑1 (MQ=255)
                                                                                   ggataAGAGTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGAGTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATc                                                        <  2:342960/139‑1 (MQ=255)
                                                                                    tataCGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTCCGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCa                                                       <  1:580525/141‑1 (MQ=255)
                                                                                     ataCGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGTCGATAATGAAACCAATCaa                                                      <  1:448372/141‑1 (MQ=255)
                                                                                      taCGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCaaa                                                     >  2:168316/1‑141 (MQ=255)
                                                                                         gATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTg                                                  <  1:21241/141‑1 (MQ=255)
                                                                                         gATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTg                                                  <  1:21333/141‑1 (MQ=255)
                                                                                               aGTTGAATGGAGTATGAATAAAAAATGCCACAAGCATATGATTAACATATGACTTTTGCATAGTAATATGATTGTGTAATTTTACTATGCTTAATTTTATAAATAGTGAAACCGATAATAAAACCAATCAAACTGAGaaca                                            >  2:110162/1‑141 (MQ=255)
                                                                                                gTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGaacaa                                           >  2:221353/1‑141 (MQ=255)
                                                                                                  ttAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAAcc                                         >  1:37858/1‑141 (MQ=255)
                                                                                                       ggcgtaTGAAAAAAAAATGCCCGAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACCACCCAGcc                                    <  2:379047/138‑1 (MQ=255)
                                                                                                         agtaTGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGa                                                                                                  <  2:665998/77‑1 (MQ=255)
                                                                                                         agtaTGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGa                                                                                                  >  1:665998/1‑77 (MQ=255)
                                                                                                          gtaTGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCAt                                 >  1:335251/1‑141 (MQ=255)
                                                                                                           taTGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATa                                <  1:103718/141‑1 (MQ=255)
                                                                                                                aTAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAAt                           >  1:30152/1‑141 (MQ=255)
                                                                                                                aTAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAAt                           >  1:290964/1‑141 (MQ=255)
                                                                                                                  aaaaaaTGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGt                         >  2:662450/1‑141 (MQ=255)
                                                                                                                    aaaaTGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCt                       <  2:302730/141‑1 (MQ=255)
                                                                                                                     aaaTGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTg                      >  1:533360/1‑141 (MQ=255)
                                                                                                                            ccaAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATgg               <  1:124947/140‑1 (MQ=255)
                                                                                                                            acaAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATgg               <  1:584291/141‑1 (MQ=255)
                                                                                                                                 cATATGTTAAACATATGCTTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAat          >  2:134558/1‑141 (MQ=255)
                                                                                                                                    atGTTAAACATATGCTTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATAtt       >  1:92135/1‑141 (MQ=255)
                                                                                                                                    atGTTAAACATATGCTTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATAtt       >  1:685139/1‑141 (MQ=255)
                                                                                                                                         tAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATATTTTTgg  >  1:248331/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
ATAAAGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATATTTTTGG  >  CP000730/216899‑217176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: