Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,575,517 G→A 6.8% A667V (GCA→GTA)  hlgB ← possible bacteriophage tail protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,575,5170GA 6.8% 177.2 / 3.1 59A667V (GCA→GTA) hlgBpossible bacteriophage tail protein
Reads supporting (aligned to +/- strand):  ref base G (30/25);  new base A (2/2);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

TGTTTGAAGACCTAAAGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTACCGACT  >  CP000730/1575378‑1575657
                                                                                                                                           |                                                                                                                                            
tGTTTGAATACCTAAAGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGc                                                                                                                                             >  2:319503/1‑141 (MQ=255)
tGTTTGAAGACCTAAAGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGc                                                                                                                                             <  1:3058/141‑1 (MQ=255)
  tttGAAGACCTAAAGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAg                                                                                                                                           >  2:541204/1‑141 (MQ=255)
    tGAAGACCTAAAGATTTCATTGCTTTTGAATTATTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGt                                                                                                                                         <  2:276427/141‑1 (MQ=255)
            tAAAGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATtgc                                                                                                                                 <  2:450900/141‑1 (MQ=255)
             aaaGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTACAGGTCCAATtgct                                                                                                                                >  1:268621/1‑141 (MQ=255)
             aaaGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTACAGGTCCAATtgct                                                                                                                                >  1:268746/1‑141 (MQ=255)
                 aTTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCAc                                                                                                                            <  1:191591/141‑1 (MQ=255)
                    tCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAAGTGCTGCACCaa                                                                                                                         >  2:437902/1‑141 (MQ=255)
                      aTTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCaaaa                                                                                                                       >  1:337556/1‑141 (MQ=255)
                          cTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTg                                                                                                                   <  2:590206/141‑1 (MQ=255)
                           ttttGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGc                                                                                                                  >  1:575872/1‑141 (MQ=255)
                            tttGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATAGCTGCACCAAAAAGTGCt                                                                                                                 <  2:317210/141‑1 (MQ=255)
                                aaTTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAAAAAAAACACAAAAAAGTGCTAATc                                                                                                             <  2:678570/141‑1 (MQ=255)
                                 aTTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATcc                                                                                                            >  2:455794/1‑141 (MQ=255)
                                      tATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTg                                                                                                       <  2:575872/141‑1 (MQ=255)
                                        tttGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTACAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGaa                                                                                                     <  2:268746/141‑1 (MQ=255)
                                        tttGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTACAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGaa                                                                                                     <  2:268621/141‑1 (MQ=255)
                                         ttGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAg                                                                                                    <  2:404825/141‑1 (MQ=255)
                                          tGAAAGGATTGTATTTTCAGCAATACGGCTATTTAATGACGCATATCCGTTAGCAGCACTTCCAACTGTACGTTTTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGc                                                                                                   >  1:510937/1‑141 (MQ=255)
                                             aaGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCttt                                                                                                >  1:458182/1‑141 (MQ=255)
                                              aGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCtttt                                                                                               <  2:337556/141‑1 (MQ=255)
                                                   tGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAAc                                                                                          <  1:504776/141‑1 (MQ=255)
                                                   tGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAAc                                                                                          <  1:341530/141‑1 (MQ=255)
                                                     tATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAAccc                                                                                        >  1:434672/1‑141 (MQ=255)
                                                        tttCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAAc                                                                                     >  2:465779/1‑141 (MQ=255)
                                                         ttCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAGGTGCTAATCCTACTGAAGCTTTTCTAACCCAAcc                                                                                    >  2:345719/1‑141 (MQ=255)
                                                         ttCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAAcc                                                                                    >  2:442406/1‑141 (MQ=255)
                                                         ttCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAAcc                                                                                    >  2:174414/1‑141 (MQ=255)
                                                         ttCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAAcc                                                                                    >  1:57237/1‑141 (MQ=255)
                                                          tCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCa                                                                                   <  1:146032/141‑1 (MQ=255)
                                                          tCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACAGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCATCCa                                                                                   <  2:606252/141‑1 (MQ=255)
                                                            aGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAgg                                                                                 <  2:179535/141‑1 (MQ=255)
                                                            aGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTNTTCTAACCCAACCAgg                                                                                 <  1:490647/141‑1 (MQ=255)
                                                              gaaTCCGTATATTAAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCATACAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTGTCCTAACCCAACCAggaa                                                                               >  2:71453/2‑139 (MQ=255)
                                                              cAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGga                                                                               >  2:41982/1‑141 (MQ=255)
                                                                aTACGTCTATTTAATGACGCATATGCTATCGCAGCACTTCCAACTGTACGTAGTAATAGCCCTCCAGCAAGAACTGCAGGTCCAATGGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGgaga                                                                             >  2:425494/1‑141 (MQ=255)
                                                                                 cGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACt                                                           >  2:378191/1‑141 (MQ=255)
                                                                                  gCATATCCAATAGCAACAATACGAACTTGACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACt                                                           <  2:136928/141‑1 (MQ=255)
                                                                                                   aCTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTc                                          >  1:18988/1‑141 (MQ=255)
                                                                                                          aCTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGCGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCcctcct                                   >  2:417486/1‑141 (MQ=255)
                                                                                                            tGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAGCCGTCCGCTCCTGc                                 <  2:49669/141‑1 (MQ=255)
                                                                                                             gTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGct                                <  2:352437/141‑1 (MQ=255)
                                                                                                             gTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAAAAGCAAAAACAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGct                                <  2:362601/141‑1 (MQ=255)
                                                                                                                cGTTTTAATAACCCTCCAGCAAGAATTGCAGGTTCAATTGTTGCACAAAAAAGGGCTAATTCTTCTGAAGTTTTTCTAACCTAACCAGGGATATGTATATATACATCAACTAATATGGTTAAACCTTCCGCTATTGCTCTa                             >  2:149374/1‑141 (MQ=255)
                                                                                                                     taataaCCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCAt                        >  1:598403/1‑141 (MQ=255)
                                                                                                                          aCCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCg                   >  1:167610/1‑141 (MQ=255)
                                                                                                                          aCCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCg                   >  1:356350/1‑141 (MQ=255)
                                                                                                                           cccTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGt                  >  1:350075/1‑141 (MQ=255)
                                                                                                                            ccTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTg                                                                                                       <  1:616881/55‑1 (MQ=255)
                                                                                                                            ccTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTg                                                                                                       >  2:616881/1‑55 (MQ=255)
                                                                                                                             cTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTa                <  1:127120/141‑1 (MQ=255)
                                                                                                                                cAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGTTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAAt             >  2:363877/1‑141 (MQ=255)
                                                                                                                                   cAAGAACTGCAGGTCCAAAAGAAACACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCtt          <  2:58062/141‑1 (MQ=255)
                                                                                                                                     aGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTa        >  1:449138/1‑141 (MQ=255)
                                                                                                                                       aaCTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTAcc      <  1:541204/141‑1 (MQ=255)
                                                                                                                                       aaCTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTAcc      >  1:324890/1‑141 (MQ=255)
                                                                                                                                       aaCTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTAcc      >  2:641358/1‑141 (MQ=255)
                                                                                                                                          tGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTACCGAc   <  2:449138/141‑1 (MQ=255)
                                                                                                                                           gCAGGTCCAGTTGCTGCACCGAAAAGTGCTAGTCGTAGTGAAGCTTTTCTAACCCAACCTGGGAGTTGTGTAAGTATATCAACTAGTGTTGTGAAACGTTCCGCTCCGGCTCTAATCGTTGGCGTTAGAGGTTTACCGGCt  >  2:2387/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TGTTTGAAGACCTAAAGATTTCATTGCTTTTGAATTAGTATTTGAAAGGATTGTATTTTCAGCAATACGTCTATTTAATGACGCATATCCTTTAGCAGCACTTCCAACTGTACGTATTAATAACCCTCCAGCAAGAACTGCAGGTCCAATTGCTGCACCAAAAAGTGCTAATCCTACTGAAGCTTTTCTAACCCAACCAGGGAGATGTGTAAATCCATCAACTAATTTTGTTAAACCTTCCGCTCCTGCTCTAATCATAGGCGTTAAATCTTTACCGACT  >  CP000730/1575378‑1575657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: