Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,894,054 A→G 6.9% L87S (TTA→TCA)  USA300HOU_1755 ← lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,894,0540AG 6.9% 148.6 / 3.5 59L87S (TTA→TCA) USA300HOU_1755lysophospholipase
Reads supporting (aligned to +/- strand):  ref base A (30/24);  new base G (2/2);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

TTTGAAACAAGTTTATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAGGCAAATCTCCCATAATC  >  CP000730/1893916‑1894191
                                                                                                                                          |                                                                                                                                         
tttGAAACAAGTTTATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAAt                                                                                                                                         <  2:522083/141‑1 (MQ=255)
        aaGTTTATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGt                                                                                                                                 <  1:29162/141‑1 (MQ=255)
          gTTTATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATaagaag                                                                                                                               >  2:612370/1‑141 (MQ=255)
           tttATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGTGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGTCAAGTACTACCCCTAATACATAAGTAgg                                                                                                                              <  2:504084/141‑1 (MQ=255)
           tttATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAgg                                                                                                                              <  2:504240/141‑1 (MQ=255)
             tATCTTTACGATTTTTTTTTTTTTTTTGAAGTTCTAGCATGGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCTAGTAAATTTAATAAAATCAAACCGCCAATTCTTACCCCTATTACATAAGTAGTaa                                                                                                                            >  2:49848/1‑141 (MQ=255)
             tATCTTTACGATTTTTTCTATTTTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAATCCTACCCCTAATACATAAGTAGGaa                                                                                                                            >  2:276567/1‑141 (MQ=255)
             tATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGaa                                                                                                                            >  1:143594/1‑141 (MQ=255)
             tATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGaa                                                                                                                            >  1:143349/1‑141 (MQ=255)
               tCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAAtt                                                                                                                          >  1:390130/1‑141 (MQ=255)
               tCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAAtt                                                                                                                          >  1:372448/1‑141 (MQ=255)
                  ttACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTta                                                                                                                       >  1:624134/1‑141 (MQ=255)
                      gATTTTTTCCATTCTTTTGACGTTCTAGCATAGGAGAAATTAACATCATCTCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATAtt                                                                                                                   >  2:390967/1‑141 (MQ=255)
                          ttttCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGACTTTTATATTCAtt                                                                                                               >  1:2060/1‑141 (MQ=255)
                          ttttCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCAtt                                                                                                               >  1:159481/1‑141 (MQ=255)
                                aTTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTAt                                                                                                         <  2:626069/141‑1 (MQ=255)
                                    ttttGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCttt                                                                                                     >  1:134723/1‑141 (MQ=255)
                                       tGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGTAATTTTATATTCATTAGCTATCTTTAAc                                                                                                  >  1:200570/1‑141 (MQ=255)
                                         aaGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCa                                                                                                >  1:158616/1‑141 (MQ=255)
                                          aGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAg                                                                                               <  1:376212/141‑1 (MQ=255)
                                             tCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTCATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTCACCAGTCt                                                                                            <  2:202753/141‑1 (MQ=255)
                                                 gCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCa                                                                                        <  1:250946/141‑1 (MQ=255)
                                                  cATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCaa                                                                                       >  2:9181/1‑141 (MQ=255)
                                                   aTAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTGATACATAAGTAGGAATTTAATATTCAGTAGCTATCTTTAACCAGTCTAGCaaa                                                                                      >  2:542503/1‑141 (MQ=255)
                                                   aTAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTGATACATAAGTAGGAATTTAATATTCAATAGCTATCTTTAACCAGTCTAGCaaa                                                                                      >  2:542530/1‑141 (MQ=255)
                                                             ttAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGAt                                                                            <  2:231658/141‑1 (MQ=255)
                                                              tAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATa                                                                           <  1:61370/141‑1 (MQ=255)
                                                              tAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATa                                                                           >  2:291181/1‑141 (MQ=255)
                                                                aCATCATCCCCTCGATTGGCAATTTTATTTTTTCAAGTATATTTAATAAAATCAAACCGCCTAGTCGTACCCCTAATACTTAAGTGGGAGTTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACg                                                                         <  2:32472/141‑1 (MQ=255)
                                                                aCATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACg                                                                         >  1:490106/1‑141 (MQ=255)
                                                                aCATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACg                                                                         <  1:360630/141‑1 (MQ=255)
                                                                aCATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACg                                                                         >  2:114062/1‑141 (MQ=255)
                                                                    catcCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTg                                                                     >  2:335914/1‑141 (MQ=255)
                                                                     atcCCCTCTATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAAACAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGa                                                                    >  1:586020/1‑141 (MQ=255)
                                                                             gATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTc                                                            <  2:523783/141‑1 (MQ=255)
                                                                             gATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTc                                                            <  1:270789/141‑1 (MQ=255)
                                                                                      ttCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGACAGTTCTCCATTTGTcc                                                   >  2:200741/1‑141 (MQ=255)
                                                                                         tACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCttt                                                <  1:9181/141‑1 (MQ=255)
                                                                                               ttCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTGATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTGTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGc                                          <  1:542503/141‑1 (MQ=255)
                                                                                               ttCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTGATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTATAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAga                                          <  1:542530/141‑2 (MQ=255)
                                                                                               ttCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTGTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCATTTTGTCCTTTATTAGc                                          >  2:617734/1‑141 (MQ=255)
                                                                                                 cAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGctc                                        <  2:158616/141‑1 (MQ=255)
                                                                                                 cAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGctc                                        <  1:390967/141‑1 (MQ=255)
                                                                                                 cAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGctc                                        <  2:462228/141‑1 (MQ=255)
                                                                                                    gTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTg                                     <  2:406155/141‑1 (MQ=255)
                                                                                                    gTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTg                                     <  2:422480/141‑1 (MQ=255)
                                                                                                    gTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTg                                     <  2:159481/141‑1 (MQ=255)
                                                                                                                      aaCCGCCAAGTCCTACCCCTAATCCATAAGAAGGAATTTTATATTCATTAGCTATCCTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAg                   >  2:486707/1‑141 (MQ=255)
                                                                                                                      aaCCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAg                   >  1:474327/1‑141 (MQ=255)
                                                                                                                      aaCCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAg                   <  1:572091/141‑1 (MQ=255)
                                                                                                                       aCCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAgg                  >  2:86646/1‑141 (MQ=255)
                                                                                                                       aCCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAgg                  >  1:587289/1‑141 (MQ=255)
                                                                                                                       aCCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAgg                  >  1:428330/1‑141 (MQ=255)
                                                                                                                       aCCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTGGCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAgg                  >  1:314447/1‑141 (MQ=255)
                                                                                                                                tCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAGGCAAATCTcc         >  2:196276/1‑141 (MQ=255)
                                                                                                                                 ccTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGCCCAGGAAAATCTgcc        >  2:379421/1‑141 (MQ=255)
                                                                                                                                 ccTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAGGCAAATCTccc        <  1:372017/141‑1 (MQ=255)
                                                                                                                                      cccTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAGGCAAATCTCCCATAAt   <  2:223137/141‑1 (MQ=255)
                                                                                                                                       ccTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGGTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAGGCAAATCTCCCATAATc  <  2:402676/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TTTGAAACAAGTTTATCTTTACGATTTTTTCCATTCTTTTGAAGTTCTAGCATAGGAGAAATTAACATCATCCCCTCGATTGGCAATTCTACTTTTTCAAGTAAATTTAATAAAATCAAACCGCCAAGTCCTACCCCTAATACATAAGTAGGAATTTTATATTCATTAGCTATCTTTAACCAGTCTAGCAAACTTTCGTGATACGTTTGAAAGTTTTCAATTTGTCCTTTATTAGCTCTTGAAGTTTGACCTTGACCAGGCAAATCTCCCATAATC  >  CP000730/1893916‑1894191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑