Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,920,410 C→A 5.7% V306L (GTG→TTG)  menC ← possible o‑succinylbenzoic acid (OSB) synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,920,4100CA 5.7% 243.1 / 3.1 70V306L (GTG→TTG) menCpossible o‑succinylbenzoic acid (OSB) synthetase
Reads supporting (aligned to +/- strand):  ref base C (32/34);  new base A (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

TCCGTTAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATGACTTTTCA  >  CP000730/1920273‑1920547
                                                                                                                                         |                                                                                                                                            
tCCGTTAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATc                                                                                                                                           <  1:357841/141‑1 (MQ=255)
  cGTTAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCtt                                                                                                                                         <  2:588262/141‑1 (MQ=255)
   gTTAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTg                                                                                                                                        >  2:70972/1‑141 (MQ=255)
   gTTAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTg                                                                                                                                        >  1:4321/1‑141 (MQ=255)
    ttAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCAAATCTTGt                                                                                                                                       >  1:563364/1‑141 (MQ=255)
    ttAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCATGAATGCGCCACCAAATCTTGt                                                                                                                                       >  1:5746/1‑141 (MQ=255)
     tAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGtt                                                                                                                                      <  1:268622/141‑1 (MQ=255)
        aaaaaGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑aa                                                                                                                                <  2:587354/141‑1 (MQ=255)
        aaaaaGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑aa                                                                                                                                <  1:305565/141‑1 (MQ=255)
              aCAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAaataat                                                                                                                          >  1:368323/1‑141 (MQ=255)
              aCAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAaataat                                                                                                                          <  2:241213/141‑1 (MQ=255)
                aaGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAAtaataa                                                                                                                        <  2:476848/141‑1 (MQ=255)
                  gTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAAcc                                                                                                                      <  1:224708/141‑1 (MQ=255)
                      gcaagcaagcaTTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACctgct                                                                                                                  >  2:546356/1‑141 (MQ=255)
                        aagcaagcaTTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTgg                                                                                                                <  1:436244/141‑1 (MQ=255)
                            atgcaTTCACATAATCATTCCCATTAATACGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGTCTGAATGCGCCACCCCATCTTGTTC‑‑‑AAAATAATACCCTGCTGGTGTa                                                                                                            <  2:392155/139‑1 (MQ=255)
                            aagcaTTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTa                                                                                                            <  2:169385/141‑1 (MQ=255)
                             agcaTTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTaa                                                                                                           <  1:398828/141‑1 (MQ=255)
                               caTTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCAAATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACa                                                                                                         <  2:563364/141‑1 (MQ=255)
                               caTTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCAAATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACa                                                                                                         <  2:5746/141‑1 (MQ=255)
                                 ttCACATAAACATAACCCTTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTTTTC‑‑‑AAAATAATAACCTGCTGGTGTAACAtc                                                                                                       <  1:39413/141‑1 (MQ=255)
                                            ataaCCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAAt                                                                                            <  2:359683/141‑1 (MQ=255)
                                             taaCCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGCCTCCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATc                                                                                           >  1:329787/1‑141 (MQ=255)
                                             taaCCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTGAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCACCTGGATAATc                                                                                           >  1:157031/1‑141 (MQ=255)
                                             taaCCATTAATATGGCTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATc                                                                                           >  2:239946/1‑141 (MQ=255)
                                               aCCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCAc                                                                                         >  2:149048/1‑141 (MQ=255)
                                               aCCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCAGGAGTAACAACTAATGGATaacggc                                                                                         <  2:187054/141‑5 (MQ=255)
                                                        atGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGag                                                                                <  1:326052/141‑1 (MQ=255)
                                                         tGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGagc                                                                               <  1:154578/141‑1 (MQ=255)
                                                             tGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGagcaagc                                                                           <  1:518980/141‑1 (MQ=255)
                                                                aaTTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATc                                                                        >  1:128749/1‑141 (MQ=255)
                                                                aaTTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATc                                                                        >  1:485203/1‑141 (MQ=255)
                                                                 aTTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCg                                                                       <  2:702375/141‑1 (MQ=255)
                                                                 aTTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCg                                                                       <  1:707850/141‑1 (MQ=255)
                                                                 aTTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCg                                                                       >  1:139243/1‑141 (MQ=255)
                                                                  ttGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGc                                                                      <  2:409331/141‑1 (MQ=255)
                                                                  ttGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGc                                                                      <  2:528323/141‑1 (MQ=255)
                                                                        tataTCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTaaa                                                                <  1:467958/141‑1 (MQ=255)
                                                                            tCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAg                                                            <  2:166722/141‑1 (MQ=255)
                                                                                cTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGc                                                        >  1:9827/1‑141 (MQ=255)
                                                                                cTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGc                                                        >  2:121017/1‑141 (MQ=255)
                                                                                cTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAACAGCgac                                                        >  2:650002/1‑139 (MQ=255)
                                                                                     ggggggCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTaaa                                                   >  1:618001/1‑141 (MQ=255)
                                                                                        gggCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCa                                                >  1:635095/1‑141 (MQ=255)
                                                                                           cgactcgaatttCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCatat                                             >  1:485319/12‑141 (MQ=255)
                                                                                            gAAATTCGAGTCGCCCCTCTTTTAATATGCCGGAATTCGCCGCCGCATCTGGTTC‑‑‑AAAATAATAACCGGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATAtt                                            <  1:239946/141‑1 (MQ=255)
                                                                                             aaaTTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTACAATAGCGGCTTAAACCATATTc                                           >  1:507064/1‑141 (MQ=255)
                                                                                               aTTCGAGTCGCCCCTCTTATAATATGCCTGAATGCGCCACAACATCTTGTTC‑‑‑AAAATAATAACGTGCTGGTGTAACATATCCTGGATAATCACCTATACGTGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATCTTCGt                                         <  2:311638/141‑1 (MQ=255)
                                                                                                 tCGAGTCGCCCCTCTTTTAATATGCCTGATTTAGTAACCACATCTTGATC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAACGGCTTAAACCATATTCATAc                                       >  1:631620/1‑141 (MQ=255)
                                                                                                 tCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTAc                                       <  2:610735/141‑1 (MQ=255)
                                                                                                   gAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACAt                                     >  2:523495/1‑141 (MQ=255)
                                                                                                       cGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATgccg                                 >  1:19096/1‑141 (MQ=255)
                                                                                                        gCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATgccgc                                >  1:76791/1‑141 (MQ=255)
                                                                                                        gCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATgccgc                                >  1:378925/1‑141 (MQ=255)
                                                                                                                tttAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAAcc                        <  2:485030/141‑1 (MQ=255)
                                                                                                                 ttAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCa                       >  1:184489/1‑141 (MQ=255)
                                                                                                                 ttAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCGCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCa                       <  2:466928/141‑1 (MQ=255)
                                                                                                                    atatGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACtt                    >  1:268155/1‑141 (MQ=255)
                                                                                                                    atatGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACtt                    <  1:184510/141‑1 (MQ=255)
                                                                                                                    atatGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACtt                    >  2:70114/1‑141 (MQ=255)
                                                                                                                      atGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACtttt                  >  2:262/1‑141 (MQ=255)
                                                                                                                        gCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAg                                                            <  2:635431/97‑1 (MQ=255)
                                                                                                                        gCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAg                                                            >  1:635431/1‑97 (MQ=255)
                                                                                                                             aaTGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCAt           >  2:166068/1‑141 (MQ=255)
                                                                                                                             aaTGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCAt           >  1:258488/1‑141 (MQ=255)
                                                                                                                              aTGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATg          >  2:254452/1‑141 (MQ=255)
                                                                                                                               tGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATGa         <  2:439605/141‑1 (MQ=255)
                                                                                                                                 cgcCACCACATCTTGTTCAAAAAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATg          <  2:374486/141‑1 (MQ=255)
                                                                                                                                 cgcCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATGACt       <  2:53022/141‑1 (MQ=255)
                                                                                                                                   ccaccaCATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATGACttt     >  1:388296/1‑141 (MQ=255)
                                                                                                                                      ccacATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATGACTTTTCa  <  2:34211/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TCCGTTAAAAAAAGACAAGTCAGCAAGCAAGCATTCACATAAACATAACCATTAATATGGTTGTAATTGAGTTATATCCACTAAAGGGGGGCGAAATTCGAGTCGCCCCTCTTTTAATATGCCTGAATGCGCCACCACATCTTGTTC‑‑‑AAAATAATAACCTGCTGGTGTAACATCTCCTGGATAATCACCTTTACGAGCAAGCATCGCTGTAAAATAGCGGCTTAAACCATATTCGTACATGCCGCCAATAACCACTTTTGCACCATGACTTTTCA  >  CP000730/1920273‑1920547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: