Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,710,898 (T)6→7 coding (29/1812 nt) oatA ← possible acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,710,8921.T91.8% 136.5 / 7.7 49coding (35/1812 nt)oatApossible acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base T (24/21);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

ACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCT  >  CP000730/2710756‑2711025
                                                                                                                                          |                                                                                                                                     
aCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACAtttt                                                                                                                                   >  1:483692/1‑141 (MQ=255)
    gAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACATTTTTTCt                                                                                                                               >  2:475774/1‑141 (MQ=255)
          aCGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGTGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACg                                                                                                                          >  2:592630/1‑141 (MQ=255)
           cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGt                                                                                                                         >  2:386142/1‑141 (MQ=255)
           cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGt                                                                                                                         >  2:322215/1‑141 (MQ=255)
             aagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGttt                                                                                                                       >  1:366366/1‑141 (MQ=255)
              agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTa                                                                                                                      <  2:183102/141‑1 (MQ=255)
              agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATGCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTa                                                                                                                      <  1:5312/141‑1 (MQ=255)
                   gTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCt                                                                                                                <  2:198522/141‑1 (MQ=255)
                    tATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCt                                                                                                                <  1:196674/141‑1 (MQ=255)
                     aTCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACCTTTTTTTCTAAACGTTTAAAGTCtt                                                                                                               >  1:447697/1‑141 (MQ=255)
                      tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCttt                                                                                                              <  2:10946/141‑1 (MQ=255)
                      tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCttt                                                                                                              >  2:119274/1‑141 (MQ=255)
                                  aaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGtt                                                                                                  <  1:92011/141‑1 (MQ=255)
                                  aaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGtt                                                                                                  >  2:301600/1‑141 (MQ=255)
                                    gCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTtaa                                                                                                >  1:514212/1‑141 (MQ=255)
                                         cACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAAc                                                                                           >  2:190823/1‑141 (MQ=255)
                                             tAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGccc                                                                                       <  2:19594/141‑1 (MQ=255)
                                                 cATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATc                                                                                                        >  1:24058/1‑120 (MQ=255)
                                                 cATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATc                                                                                                        <  2:24058/120‑1 (MQ=255)
                                                  aTTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCTATGTTAATAAACGCCCCattt                                                                                  >  1:346894/1‑141 (MQ=255)
                                                       gCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAg                                                                                                                   <  1:474515/103‑1 (MQ=255)
                                                       gCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAg                                                                                                                   >  2:474515/1‑103 (MQ=255)
                                                           tCAAGTGATAAATAATGTTTTTTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtctc                                                                         <  1:153580/141‑1 (MQ=255)
                                                                tGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAttt                                                                    >  2:41601/1‑141 (MQ=255)
                                                                        aatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTg                                                                  >  1:296108/1‑135 (MQ=255)
                                                                        aatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTg                                                                  <  2:296108/135‑1 (MQ=255)
                                                                           gATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATca                                                        >  1:305498/1‑141 (MQ=255)
                                                                              tCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATcaca                                                      >  1:537643/1‑141 (MQ=255)
                                                                               ccTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                     <  2:178797/141‑1 (MQ=255)
                                                                                    aaCTGCGAATGCACTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATAttt                                                >  2:240516/1‑141 (MQ=255)
                                                                                     aCTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATAtttt                                               >  1:251704/1‑141 (MQ=255)
                                                                                      cTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATAttttt                                              <  1:375611/141‑1 (MQ=255)
                                                                                         cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCCTTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaa                                           >  1:207496/1‑141 (MQ=255)
                                                                                             tGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaata                                       <  1:186252/141‑1 (MQ=255)
                                                                                                    aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                >  1:418203/1‑141 (MQ=255)
                                                                                                    aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                >  1:478013/1‑141 (MQ=255)
                                                                                                    aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATGGATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                >  1:116119/1‑141 (MQ=255)
                                                                                                     aTCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTg                               >  2:17174/1‑141 (MQ=255)
                                                                                                         aTCTAATACAGGTAAGTATCGCGGGGTATACATTTTTTTCTAACCGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                           <  2:235265/141‑1 (MQ=255)
                                                                                                          tctcATCCCGCTAAGTATCGCGGGGAATAGATTTTTTTCTAAACCTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCTCATTTATTTTTCTCTATTTTGGAGCATATCACAATATTTTTGAAAATAAAATATTGCACTg                          <  2:555277/141‑1 (MQ=255)
                                                                                                             aaTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                       <  1:190823/141‑1 (MQ=255)
                                                                                                                 cAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCa                   <  1:476698/141‑1 (MQ=255)
                                                                                                                   ggTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTTCACTGTTTTTCttt                 >  2:159377/1‑141 (MQ=255)
                                                                                                                            cgcgGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGAttt        <  1:592630/141‑1 (MQ=255)
                                                                                                                             gcgGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTa       >  2:100768/1‑141 (MQ=255)
                                                                                                                             gcgGGGAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTa       <  1:17285/141‑1 (MQ=255)
                                                                                                                                 gtaaTACATTTTTTTCTAAACGTTGAAAGTCTTTTGTATCCATTTTAATAGACGCCCCATTTATTTTTGTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAGccc   <  2:514212/139‑1 (MQ=255)
                                                                                                                                 ggAATACATTTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAATAATTGATTTAAccc   >  1:359124/1‑141 (MQ=255)
                                                                                                                                  gAATACATTTTTTTCTAAACGTTTAAAGTCGTTTGTATTCATGTAAATAAACGGCCCATTTTTTTTTCTCTATTTGTTAGCATATCATCATACTTTTGAAAATAAAATATTGCACTCATTTTCATTAATTGATTTAACCCt  <  2:60001/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
ACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCT  >  CP000730/2710756‑2711025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: