New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 20803627 (0.780)5 (0.150) 5/252 4.1 15.2% coding (4445/7194 nt) USA300HOU_0192 non‑ribosomal peptide synthetase
?CP000730 = 208062 30 (0.920)coding (4471/7194 nt) USA300HOU_0192 non‑ribosomal peptide synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTTAATGAAGTTGTCTCACGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCGAATAAACGACAAGGTGCAATTTTGGAG  >  CP000730/207935‑208202
                                                                                                                               |                                                                                                                                            
cTTTAATGAAGTTGTCTCACGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACg                                                                                                                                 <  2:157279/141‑1 (MQ=255)
     aTGAAGTTGTCTCACTACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTg                                                                                                                            >  1:313406/1‑141 (MQ=255)
          gTTGTCTCACGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTg                                                                                                                       >  2:101431/1‑141 (MQ=255)
          gTTGTCTCACGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTg                                                                                                                       >  1:14443/1‑141 (MQ=255)
           ttgtcgtgagACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGt                                                                                                                      >  1:64511/10‑141 (MQ=255)
           ttGTCTCACGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGt                                                                                                                      <  1:351564/141‑1 (MQ=255)
                   cGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGaa                                                                                                              >  2:266219/1‑141 (MQ=255)
                       aaGGTAGTCATGTTTCGCTAGTCATGAATGATTTTATACTGACGTATGAAACATTACGTAGCTATGTGGATGCCATTGCGCACAGGCTCCTACCAAATACTGTGGGTAATCGGCAACGGGTCTCCTTGTTTACAGAACGTa                                                                                                          <  2:209578/141‑1 (MQ=255)
                       aaGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTa                                                                                                          <  2:27826/141‑1 (MQ=255)
                          gTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGtt                                                                                                       >  1:180978/1‑141 (MQ=255)
                             aTCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTg                                                                                                    <  2:14443/141‑1 (MQ=255)
                                  gTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACGATGTGGATGCGATTGCGCCAATGCTCCTATAACATGGTGCGGGCCATGGCCAACGGGTTGCCTTGCTTACAGAACGTAGtaatgcaagg                                                                                               >  2:388800/1‑132 (MQ=255)
                                   ttGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGa                                                                                              <  2:64511/141‑1 (MQ=255)
                                            tCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCCAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGa                                                                                     <  1:131099/141‑1 (MQ=255)
                                                gaatgaTTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTAAAGAACGGAGTTTTGATATGATTTCGGCGATGtt                                                                                 >  1:124863/1‑141 (MQ=255)
                                                            aaTGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTaa                                                                     <  2:408746/141‑1 (MQ=255)
                                                                  gTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAgg                                                               >  1:315473/1‑141 (MQ=255)
                                                                     tGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAgatgcatg                                                            >  1:360360/1‑134 (MQ=255)
                                                                                       tgtgGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGa                                          <  2:428160/141‑1 (MQ=255)
                                                                                          ggATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGatat                                       <  2:53881/141‑1 (MQ=255)
                                                                                                   aaagtgttatcTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTcc                              <  2:111410/131‑1 (MQ=255)
                                                                                                    gcgcACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCg                             >  1:84363/1‑141 (MQ=255)
                                                                                                    gcgcACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCg                             <  2:118212/141‑1 (MQ=255)
                                                                                                    gcgcACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCg                             >  1:429841/1‑141 (MQ=255)
                                                                                                     cgcACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGaaa                                                                                                 <  2:245495/72‑1 (MQ=255)
                                                                                                     cgcACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGaaa                                                                                                 >  1:245495/1‑72 (MQ=255)
                                                                                                        acaTGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCGAATa                         >  1:382563/1‑141 (MQ=255)
                                                                                                          aTGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCGAATaaa                       <  2:245999/141‑1 (MQ=255)
                                                                                                                    cAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCGAATAAACGACAAGGTg             <  2:313406/141‑1 (MQ=255)
                                                                                                                               ggCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCGAATAAACGACAAGGTGCAATTTTggag  >  1:232830/1‑141 (MQ=255)
                                                                                                                               |                                                                                                                                            
CTTTAATGAAGTTGTCTCACGACAAGGTAATCATGTTGCGCTAGTCATGAATGATTTGACAATGACGTATGAAACATTACGCAACTATGTGGATGCCATTGCGCACATGCTCCTATCAAATGGTGTGGGCAATGGTCAACGGGTTGCCTTGTTTACAGAACGTAGTTTTGAAATGATTGCGGCGATGTTGGCGACAGTTAAAGTAGGTGCATCTTATATACCTATCGATATTGATTTTCCGAATAAACGACAAGGTGCAATTTTGGAG  >  CP000730/207935‑208202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.