Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,372,946 G→T A277D (GCT→GAT)  pbuG ← NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,372,9460GT98.7% 255.3 / NA 78A277D (GCT→GAT) pbuGNCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (39/38);  minor base C (1/0);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATA  >  CP000730/2372809‑2373083
                                                                                                                                         |                                                                                                                                          
cacCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCaa                                                                                                                                         >  2:134778/1‑141 (MQ=255)
    tGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGc                                                                                                                                     <  1:497361/141‑1 (MQ=255)
      aaGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGcta                                                                                                                                   >  2:685292/1‑141 (MQ=255)
      aaGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGcta                                                                                                                                   >  2:685287/1‑141 (MQ=255)
        gTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGctact                                                                                                                                 >  1:560875/1‑141 (MQ=255)
         ttGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGctacta                                                                                                                                >  1:188824/1‑141 (MQ=255)
            aTTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATg                                                                                                                             >  1:358989/1‑141 (MQ=255)
             ttCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTATAGAATGTGAGAAAAGTTCGCTGCCAGCGCTGGGGAATTTATTATCGTTCATAATACCTGCTTGAGTACCAACAGCGACTAATGt                                                                                                                           >  1:915780/1‑141 (MQ=255)
                  tataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAg                                                                                                                      >  2:593211/1‑141 (MQ=255)
                   ataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                     <  1:561407/141‑1 (MQ=255)
                   ataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                     <  1:128335/141‑1 (MQ=255)
                   ataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                     <  1:842810/141‑1 (MQ=255)
                          gttgttGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCa                                                                                                              >  2:762294/1‑141 (MQ=255)
                            tgttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCaaa                                                                                                            >  1:205445/1‑141 (MQ=255)
                               tgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGaa                                                                                                         >  1:341094/1‑141 (MQ=255)
                                gtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                     <  2:85192/128‑1 (MQ=255)
                                gtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                     >  1:85192/1‑128 (MQ=255)
                                gtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGaaa                                                                                                        <  2:189032/141‑1 (MQ=255)
                                    ttgttCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCaa                                                                                                    <  1:505696/141‑1 (MQ=255)
                                              gtatGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGaa                                                                                          >  2:192377/4‑141 (MQ=255)
                                               aCTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAAc                                                                                         >  1:520015/1‑141 (MQ=255)
                                                cTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCATCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACg                                                                                        <  1:420637/141‑1 (MQ=255)
                                                cTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACg                                                                                        >  1:821537/1‑141 (MQ=255)
                                                    tCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTaa                                                                                    >  2:161400/1‑141 (MQ=255)
                                                    tCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTaa                                                                                    >  2:262250/1‑141 (MQ=255)
                                                     ccTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTaaa                                                                                   <  2:136385/141‑1 (MQ=255)
                                                       tACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAAt                                                                                 <  1:2890/141‑1 (MQ=255)
                                                       tACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAAGGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAAt                                                                                 <  2:180406/141‑1 (MQ=255)
                                                         cAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATaa                                                                               >  1:916962/1‑141 (MQ=255)
                                                            tcctAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACaa                                                                            <  2:738635/138‑1 (MQ=255)
                                                            tCGTAGCTAACGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACaa                                                                            >  2:562633/1‑141 (MQ=255)
                                                            tCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACaa                                                                            <  2:218832/141‑1 (MQ=255)
                                                             cGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAAt                                                                           >  1:703572/1‑141 (MQ=255)
                                                                   tAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGaa                                                                     <  1:292052/141‑1 (MQ=255)
                                                                   tAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGaa                                                                     <  1:294927/141‑1 (MQ=255)
                                                                       gAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGt                                                                 <  2:720153/141‑1 (MQ=255)
                                                                        aaTCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTa                                                                <  1:713739/141‑1 (MQ=255)
                                                                         aTCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTAt                                                               >  1:794566/1‑141 (MQ=255)
                                                                         aTCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTAt                                                               <  1:38464/141‑1 (MQ=255)
                                                                             gagaACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGtt                                                           >  1:437251/1‑141 (MQ=255)
                                                                              agaACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTta                                                          >  1:809180/1‑141 (MQ=255)
                                                                                  cAATGCTCTACCAGCTCGTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTtaataa                                                      <  2:603282/141‑1 (MQ=255)
                                                                                     tGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTg                                                   >  1:356994/1‑141 (MQ=255)
                                                                                      gCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGa                                                  >  1:891572/1‑141 (MQ=255)
                                                                                      gCTCTACCAGCTCTGGGTAATTTTTAATTTTCAAAAATACCGGCTTGCGCATCACCACTACTTATGTTACCACCGGTATCAAAGAAATCAATAAATAAGAACGTTAAAATACCAATGAAGAACTGTACCGTAAAAAATTGa                                                  >  2:596509/1‑141 (MQ=255)
                                                                                       ctctACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGAc                                                 <  2:358989/141‑1 (MQ=255)
                                                                                        tctACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACt                                                <  2:560875/141‑1 (MQ=255)
                                                                                           aCCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTgg                                             <  1:73735/141‑1 (MQ=255)
                                                                                            ccAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTggg                                            >  1:249563/1‑141 (MQ=255)
                                                                                             cAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGt                                           <  1:673327/141‑1 (MQ=255)
                                                                                              aGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTc                                          >  1:338161/1‑141 (MQ=255)
                                                                                                ctctTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCtt                                        <  1:384516/141‑1 (MQ=255)
                                                                                                 tctTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCttt                                       <  2:191471/141‑1 (MQ=255)
                                                                                                    tGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTaaa                                    >  2:398091/1‑141 (MQ=255)
                                                                                                       tAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGc                                 <  2:156224/141‑1 (MQ=255)
                                                                                                        aaTTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCt                                <  1:285989/141‑1 (MQ=255)
                                                                                                        aaTTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCt                                <  2:511833/141‑1 (MQ=255)
                                                                                                          tttATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTc                              >  1:211889/1‑141 (MQ=255)
                                                                                                                    ttCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATgcagca                    <  2:891572/141‑1 (MQ=255)
                                                                                                                     tCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCAc                   >  2:904578/1‑141 (MQ=255)
                                                                                                                     tCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCAc                   <  2:520015/141‑1 (MQ=255)
                                                                                                                      cATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCAcc                  >  1:753844/1‑141 (MQ=255)
                                                                                                                      cATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCAcc                  >  2:700589/1‑141 (MQ=255)
                                                                                                                        taataCCTGCTTGAGAATATCCATCTACTTATGTATCTGCTGTATCAATGAAACCAATATATAAGAACGTTAAAATAACAATTAAGCACTGTATCGTTAATAATTGACTTGAGTCTTTAAACGCTTCAAATGCAGCACCGa                <  1:100201/141‑1 (MQ=255)
                                                                                                                           taCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACg             >  2:883499/1‑141 (MQ=255)
                                                                                                                             ccTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGtt           >  2:809541/1‑141 (MQ=255)
                                                                                                                              cTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTgg         >  2:290595/1‑141 (MQ=255)
                                                                                                                                gCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGt        >  2:481327/1‑141 (MQ=255)
                                                                                                                                gCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGt        <  1:158954/141‑1 (MQ=255)
                                                                                                                                gCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTGCTAATGCAGCACCGAACGTTGGt        >  2:37542/1‑141 (MQ=255)
                                                                                                                                 cTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGtt       <  2:821537/141‑1 (MQ=255)
                                                                                                                                 cTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGtt       <  2:116997/141‑1 (MQ=255)
                                                                                                                                  ttGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAACCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTc      <  1:398200/141‑1 (MQ=255)
                                                                                                                                   tGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCa     >  2:193484/1‑141 (MQ=255)
                                                                                                                                   tGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCa     <  2:500371/141‑1 (MQ=255)
                                                                                                                                    gAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCaa    <  2:481784/141‑1 (MQ=255)
                                                                                                                                     aGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAt   >  1:612283/1‑141 (MQ=255)
                                                                                                                                      gTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATa  <  1:206758/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAATA  >  CP000730/2372809‑2373083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: