Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,797,717 Δ1 bp 5.2% intergenic (+5/+262) acmB → / ← USA300HOU_2645 possible N‑acetylmuramoyl‑L‑alanine amidase/possible isochorismatase hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,797,7170T. 5.2% 309.5 / 10.1 97intergenic (+5/+262)acmB/USA300HOU_2645possible N‑acetylmuramoyl‑L‑alanine amidase/possible isochorismatase hydrolase
Reads supporting (aligned to +/- strand):  ref base T (50/42);  new base . (2/3);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AGCATTTGTTGAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTA  >  CP000730/2797577‑2797857
                                                                                                                                            |                                                                                                                                            
aGCATTTGTTGAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTATATAAGtatt                                                                                                                                              <  1:952719/141‑1 (MQ=255)
 gCATTTGTTGAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGtatta                                                                                                                                             <  1:803628/141‑1 (MQ=255)
 gCATTTGTTGAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGtatta                                                                                                                                             >  1:749887/1‑141 (MQ=255)
    tttGTTGAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGtattatat                                                                                                                                          <  2:884505/141‑1 (MQ=255)
       gttgAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTaa                                                                                                                                       >  2:945087/1‑141 (MQ=255)
        ttgAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTaaa                                                                                                                                      <  2:314566/141‑1 (MQ=255)
        ttgAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTaaa                                                                                                                                      <  1:997398/141‑1 (MQ=255)
         tgAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAAc                                                                                                                                     >  2:771923/1‑141 (MQ=255)
           aaaaaGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAAccc                                                                                                                                   >  1:661736/1‑141 (MQ=255)
             aaaGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATAGATTACAGGTAAATAAGTATTATATTAAACCCGt                                                                                                                                 <  1:513580/141‑1 (MQ=255)
               aGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaa                                                                                                                               >  2:996020/1‑141 (MQ=255)
               aGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaa                                                                                                                               <  1:802199/141‑1 (MQ=255)
                gTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaaa                                                                                                                              >  1:284994/1‑141 (MQ=255)
                gTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaaa                                                                                                                              <  2:258230/141‑1 (MQ=255)
                gTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaaa                                                                                                                              >  2:336396/1‑141 (MQ=255)
                  tAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAAt                                                                                                                            >  2:1036358/1‑141 (MQ=255)
                           tGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGta                                                                                                                   >  1:806184/1‑141 (MQ=255)
                                cTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGATCTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAAc                                                                                                              <  2:970654/141‑1 (MQ=255)
                                cTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAAc                                                                                                              <  2:662047/141‑1 (MQ=255)
                                cTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAAc                                                                                                              <  2:684651/141‑1 (MQ=255)
                                   tCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAg                                                                                                           >  1:530961/1‑141 (MQ=255)
                                       tATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGt                                                                                                       <  1:116030/141‑1 (MQ=255)
                                        aTTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGtt                                                                                                      >  2:278160/1‑141 (MQ=255)
                                          ttCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCg                                                                                                    <  1:598534/141‑1 (MQ=255)
                                            cAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGa                                                                                                  >  1:483897/1‑141 (MQ=255)
                                              gAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACt                                                                                                >  2:334980/1‑141 (MQ=255)
                                              aaaTCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTACATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACt                                                                                                <  2:363228/140‑1 (MQ=255)
                                               aaTCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaa                                                                                                                               <  2:858296/109‑1 (MQ=255)
                                               aaTCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTaa                                                                                                                               >  1:858296/1‑109 (MQ=255)
                                               aaTCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACtt                                                                                               >  2:577390/1‑141 (MQ=255)
                                                 tCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTaa                                                                                             <  1:704175/141‑1 (MQ=255)
                                                 tCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTaa                                                                                             >  1:705463/1‑141 (MQ=255)
                                                 tCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACAATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTaa                                                                                             <  2:473020/141‑1 (MQ=255)
                                                  ccAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTaaa                                                                                            <  2:30352/141‑1 (MQ=255)
                                                   cAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTaaaa                                                                                           >  2:701603/1‑140 (MQ=255)
                                                    aaTGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACa                                                                                          >  2:1002380/1‑141 (MQ=255)
                                                        ttAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATAtt                                                                                      <  2:308645/141‑1 (MQ=255)
                                                            aGGATTAGGTATTATTTCTCATAGAATTATTAACGCAGCTGTAGATGAAGAATAATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTg                                                                                  <  1:822288/141‑1 (MQ=255)
                                                            aGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGt                                                                                                                    >  2:980280/1‑107 (MQ=255)
                                                            aGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGt                                                                                                                    <  1:980280/107‑1 (MQ=255)
                                                             ggATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGt                                                                                 >  1:629015/1‑141 (MQ=255)
                                                             ggATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGt                                                                                 >  1:586384/1‑141 (MQ=255)
                                                              gATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGtt                                                                                <  2:978383/141‑1 (MQ=255)
                                                              gATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGtt                                                                                <  1:1078748/141‑1 (MQ=255)
                                                                         aTTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGa                                                                     >  2:594478/1‑141 (MQ=255)
                                                                            tctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACTGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGAttt                                                                  <  2:685109/141‑1 (MQ=255)
                                                                            tctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGAttt                                                                  <  1:966205/141‑1 (MQ=255)
                                                                            tctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGAttt                                                                  <  2:385390/141‑1 (MQ=255)
                                                                            tctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGAttt                                                                  <  2:227442/141‑1 (MQ=255)
                                                                             ctcATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTc                                                                 >  2:904272/1‑141 (MQ=255)
                                                                               cATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCtt                                                               >  2:579474/1‑141 (MQ=255)
                                                                                 tAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATGAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAt                                                             <  2:124595/141‑1 (MQ=255)
                                                                                 tAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAt                                                             <  1:141695/141‑1 (MQ=255)
                                                                                  aGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAtt                                                            >  2:957835/1‑141 (MQ=255)
                                                                                  aGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTAtt                                                            >  1:311401/1‑141 (MQ=255)
                                                                                  aGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCCTAtt                                                            >  2:560023/1‑141 (MQ=255)
                                                                                   gAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTc                                                           >  1:1082785/1‑141 (MQ=255)
                                                                                       tATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAAtt                                                       <  2:910010/141‑1 (MQ=255)
                                                                                         tCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTGTAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaa                                                     >  2:294387/1‑141 (MQ=255)
                                                                                          cAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaaa                                                    >  2:35904/1‑141 (MQ=255)
                                                                                          cAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaaa                                                    <  2:379573/141‑1 (MQ=255)
                                                                                          cAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTaaa                                                    >  2:444094/1‑141 (MQ=255)
                                                                                           aatgCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAAccc                                                 >  2:690685/4‑141 (MQ=255)
                                                                                           aaCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAc                                                   >  1:930865/1‑141 (MQ=255)
                                                                                            aCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAcc                                                  <  1:148916/141‑1 (MQ=255)
                                                                                            aCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAcc                                                  >  2:495835/1‑141 (MQ=255)
                                                                                            aCGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTAATAAGTCTGATTTCTTATTCAATTAAAcc                                                  <  1:472895/141‑1 (MQ=255)
                                                                                             cGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAccc                                                 <  1:673431/141‑1 (MQ=255)
                                                                                             cGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAAccc                                                 >  2:722438/1‑141 (MQ=255)
                                                                                                agcggcagCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAg                                              >  2:515319/8‑141 (MQ=255)
                                                                                                 gCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAGCCCGAgg                                             <  2:991487/141‑1 (MQ=255)
                                                                                                 gCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAgg                                             <  1:518453/141‑1 (MQ=255)
                                                                                                   tGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGTAtt                                         >  1:835417/1‑141 (MQ=39)
                                                                                                    gCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCAt                                          >  2:1026604/1‑141 (MQ=255)
                                                                                                     ccGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCAtt                                         >  2:477989/1‑141 (MQ=255)
                                                                                                      cGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTc                                        <  1:286579/141‑1 (MQ=255)
                                                                                                      cGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTc                                        >  2:461619/1‑141 (MQ=255)
                                                                                                          gaagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGtt                                    >  1:1018120/1‑141 (MQ=255)
                                                                                                          gaagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGtt                                    >  1:1018698/1‑141 (MQ=255)
                                                                                                           aagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTc                                   <  2:871908/141‑1 (MQ=255)
                                                                                                           aagaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTc                                   <  2:930281/141‑1 (MQ=255)
                                                                                                           aagaaTTATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactga                                 <  1:623925/141‑8 (MQ=255)
                                                                                                             gaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactga                                 <  1:1048199/141‑8 (MQ=255)
                                                                                                             gaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactga                                 <  2:971120/141‑8 (MQ=255)
                                                                                                             gaaTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGa                                 <  2:978982/141‑1 (MQ=255)
                                                                                                               aTTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCGTTcgaactgaat                               <  1:673422/141‑10 (MQ=255)
                                                                                                                ttATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCCGATTTCTTATTCAATTAAACCCGAGGTATTCAGTTCGAACGcc                            <  2:1060832/141‑1 (MQ=39)
                                                                                                                 tATCATATATTACAGGTAAATAAGTAT‑‑TATTAAACCCGCAAAATTTAAAAGTATAAACAAGGAGTTCGGTCTTAAACATATTTCTGTTCATAAGTCCGAGTTCTTATTCAATTAAACCCGAGGCGTTcgaactgaatacct                           <  1:718075/141‑14 (MQ=255)
                                                                                                                  aTCATATATCACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGcc                            >  1:953284/1‑141 (MQ=255)
                                                                                                                       atatTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCggg                       <  1:1005938/141‑1 (MQ=255)
                                                                                                                       atatTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCggg                       >  2:459866/1‑141 (MQ=255)
                                                                                                                       atatTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACACGAGGCATTCAGTTCGAACGCCTCggg                       >  2:1057006/1‑141 (MQ=255)
                                                                                                                              aGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGtttt                >  1:148743/1‑141 (MQ=255)
                                                                                                                              aGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGtttt                >  1:379595/1‑141 (MQ=255)
                                                                                                                                  aaaTAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTtatat            >  2:826793/1‑141 (MQ=255)
                                                                                                                                  aaaTAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTtatat            >  1:745337/1‑141 (MQ=255)
                                                                                                                                   aaTAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATATGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGTGTCGTTTtatata           >  1:758663/1‑141 (MQ=255)
                                                                                                                                       aGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAata       >  1:622519/1‑141 (MQ=255)
                                                                                                                                         tattatattaAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAatata     >  2:385297/1‑141 (MQ=255)
                                                                                                                                            tatattaAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATAtatta  >  1:517559/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AGCATTTGTTGAAAAAGTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATTTCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAAGTATTATATTAAACCCGTAAAATTTATAAGTATAAACAAGGAGTTCGGACTTAAACATATTTCTGTTCATAAGTCTGATTTCTTATTCAATTAAACCCGAGGCATTCAGTTCGAACGCCTCGGGTCGTTTTATATAAATATATTA  >  CP000730/2797577‑2797857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: