Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 632,109 C→G 6.2% V78V (GTC→GTG proP → MFS family major facilitator transporter, proline/betaine:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730632,1090CG 6.2% 376.0 / 15.1 112V78V (GTC→GTGproPMFS family major facilitator transporter, proline/betaine:cation symporter
Reads supporting (aligned to +/- strand):  ref base C (47/58);  new base G (3/4);  total (50/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 115 total aligned reads displayed.

TTCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTATTATT  >  CP000730/631969‑632249
                                                                                                                                            |                                                                                                                                             
ttCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTc                                                                                                                                               <  1:552075/141‑1 (MQ=255)
ttCGATTTTGATGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAATTGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTc                                                                                                                                               <  2:102933/141‑1 (MQ=255)
 tCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCATACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCg                                                                                                                                              <  2:58124/141‑1 (MQ=255)
 tCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCg                                                                                                                                              >  1:618191/1‑141 (MQ=255)
  cGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGt                                                                                                                                             >  2:935185/1‑141 (MQ=255)
   gATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTa                                                                                                                                            <  2:193741/141‑1 (MQ=255)
     ttttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTAtt                                                                                                                                          <  1:58010/141‑1 (MQ=255)
     ttttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTAtt                                                                                                                                          <  1:861613/141‑1 (MQ=255)
     ttttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTAtt                                                                                                                                          >  1:898853/1‑141 (MQ=255)
     ttttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTAtt                                                                                                                                          <  1:387580/141‑1 (MQ=255)
       ttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTg                                                                                                                                        <  1:988577/141‑1 (MQ=255)
       ttGGTGGCTATGCATATACAACTGCGTACGTTGGCGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGGAGCATTAGCCATTGCGTTTTTATTAAGCCCAATTGGTGGTGTCGTATTTg                                                                                                                                        <  2:640879/141‑1 (MQ=255)
         ggTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGt                                                                                                                                      <  1:534136/141‑1 (MQ=255)
               tATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTattatt                                                                                                                                <  1:181283/141‑1 (MQ=255)
                aTGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTg                                                                                                                               <  1:428694/141‑1 (MQ=255)
                 tGCATATACATCTGCGTACAGTGGAGCGAACTTCTCCGCTCCAGTAGAGAATGCAGACACTCGACATATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTgg                                                                                                                              <  2:239263/141‑1 (MQ=255)
                  gCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTGGTATTTGGTATTATTGGt                                                                                                                             <  2:683789/141‑1 (MQ=255)
                      ataCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACa                                                                                                                         >  2:224336/1‑141 (MQ=255)
                      ataCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACa                                                                                                                         <  1:31044/141‑1 (MQ=255)
                        aCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGGATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACaaa                                                                                                                       >  1:689534/1‑141 (MQ=255)
                        aCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACaaa                                                                                                                       >  2:740722/1‑141 (MQ=255)
                           aCTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAatat                                                                                                                    <  2:312859/141‑1 (MQ=255)
                             tGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATgg                                                                                                                  >  2:822114/1‑141 (MQ=255)
                              gCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGa                                                                                                                 <  1:649084/141‑1 (MQ=255)
                              gCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGa                                                                                                                 <  2:731133/141‑1 (MQ=255)
                              gCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGa                                                                                                                 <  2:963280/141‑1 (MQ=255)
                                gTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACg                                                                                                               <  2:416537/141‑1 (MQ=255)
                                gTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACg                                                                                                               <  2:295732/141‑1 (MQ=255)
                                         aGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGt                                                                                                      <  2:250871/141‑1 (MQ=255)
                                         aGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGt                                                                                                      <  2:307092/141‑1 (MQ=255)
                                            gaagaagttcgctcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTAtt                                                                                                   >  2:298510/12‑141 (MQ=255)
                                             aagaagttcgctcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTa                                                                                                  >  1:328764/11‑141 (MQ=255)
                                                  cttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATc                                                                                             <  1:397136/141‑1 (MQ=255)
                                                  cttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATc                                                                                             >  2:405787/1‑141 (MQ=255)
                                                   ttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAAACCAATTGGTGGTGTCGTATATGGTATTATTGGTGACAAATATGGACGTAAAGTTGTTTTAACATCt                                                                                            >  1:368359/1‑141 (MQ=255)
                                                    tctctcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTa                                                                                           >  1:543041/1‑141 (MQ=255)
                                                     ctctcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACACATATGGACGTAAAGTTGTATTAACATCTAc                                                                                          <  2:975340/141‑1 (MQ=255)
                                                        tcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAat                                                                                       <  2:898853/141‑1 (MQ=255)
                                                         ccAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTCTTAACATCTACAatt                                                                                      >  2:71803/1‑141 (MQ=255)
                                                         ccAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAatt                                                                                      <  1:439290/141‑1 (MQ=255)
                                                             tAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTAttt                                                                                 >  2:392124/1‑141 (MQ=255)
                                                             tAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTAttt                                                                                 >  2:457420/1‑141 (MQ=255)
                                                              agagAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTAtttt                                                                                >  1:197776/1‑141 (MQ=255)
                                                              agagAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTAtttt                                                                                >  1:197822/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             <  1:117012/141‑1 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             >  1:765364/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             >  2:719169/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             <  2:1560/141‑1 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             >  2:754139/1‑141 (MQ=255)
                                                                   aTGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATgg                                                                           <  1:101523/141‑1 (MQ=255)
                                                                   aTGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATgg                                                                           <  2:936852/141‑1 (MQ=255)
                                                                    tGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATggt                                                                          <  1:1031629/141‑2 (MQ=255)
                                                                    tGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGc                                                                          <  1:196390/141‑1 (MQ=255)
                                                                        gACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCAttt                                                                      >  2:115330/1‑141 (MQ=255)
                                                                           aTTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCa                                                                   >  2:244562/1‑141 (MQ=255)
                                                                             tCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAAc                                                                 <  1:821904/141‑1 (MQ=255)
                                                                              cGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACa                                                                <  1:1019155/141‑1 (MQ=255)
                                                                              cGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACa                                                                >  2:984812/1‑141 (MQ=255)
                                                                               gACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACAt                                                               >  1:403425/1‑141 (MQ=255)
                                                                                aCAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACAtt                                                              >  1:643144/1‑141 (MQ=255)
                                                                                aCAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACAtt                                                              <  1:318173/141‑1 (MQ=255)
                                                                                 cttatattgacacgcGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTa                                                             <  2:856153/127‑1 (MQ=255)
                                                                                 cAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTa                                                             <  2:618191/141‑1 (MQ=255)
                                                                                  aaaTGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTaa                                                            >  1:188765/1‑141 (MQ=255)
                                                                                    aTGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAAcc                                                          <  1:993982/141‑1 (MQ=255)
                                                                                      tctGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCAt                                                        <  1:320992/139‑1 (MQ=255)
                                                                                       ttGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCAtt                                                       <  1:740722/141‑1 (MQ=255)
                                                                                        tGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTg                                                      >  1:1022231/1‑141 (MQ=255)
                                                                                         gACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTgg                                                     >  1:55699/1‑141 (MQ=255)
                                                                                         gACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTgg                                                     >  1:502457/1‑141 (MQ=255)
                                                                                         gACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTgg                                                     >  1:364741/1‑141 (MQ=255)
                                                                                          aCTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGa                                                    <  1:106934/141‑1 (MQ=255)
                                                                                             ttCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGatta                                                 <  2:382203/141‑1 (MQ=255)
                                                                                                gcagcaTTAGCCATTGCGTTTTTATTAAGACCAATTGGCGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTg                                              >  1:812477/1‑141 (MQ=255)
                                                                                                    caTTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCaa                                          <  1:803741/141‑1 (MQ=255)
                                                                                                    caTTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCaa                                          >  1:576885/1‑141 (MQ=255)
                                                                                                      ttAGCCATTGCGTTTTTNTTAAGACCAATTGGTNGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGc                                        >  1:705533/1‑141 (MQ=255)
                                                                                                      ttAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAg                                         >  2:822470/1‑141 (MQ=255)
                                                                                                        aGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTa                                      >  2:779422/1‑141 (MQ=255)
                                                                                                         gCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTAt                                     <  2:797083/141‑1 (MQ=255)
                                                                                                                     ttattaAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGa                         <  1:935185/141‑1 (MQ=255)
                                                                                                                      tattaAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGAc                        <  2:528700/141‑1 (MQ=255)
                                                                                                                          aaGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTg                    >  1:167023/1‑141 (MQ=255)
                                                                                                                          aaGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTg                    <  1:147261/141‑1 (MQ=255)
                                                                                                                              ccAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCa                <  2:156040/141‑1 (MQ=255)
                                                                                                                                aaTTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCAcc              >  2:939787/1‑141 (MQ=255)
                                                                                                                                 aTTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCa             >  2:213106/1‑141 (MQ=255)
                                                                                                                                   tggtggTGTCGTATTTGGTATTATTGGTGACAAATATGGGCGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAAt           <  2:457616/141‑1 (MQ=255)
                                                                                                                                      tggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTACCCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACt        <  1:919719/141‑1 (MQ=255)
                                                                                                                                      tggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACt        >  2:59512/1‑141 (MQ=255)
                                                                                                                                      tggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACt        <  2:849091/141‑1 (MQ=255)
                                                                                                                                       ggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACta       >  1:428519/1‑141 (MQ=255)
                                                                                                                                        gtgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtat      <  2:510676/141‑1 (MQ=255)
                                                                                                                                        gtgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtat      >  2:198742/1‑141 (MQ=255)
                                                                                                                                         tgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtatt     >  2:412272/1‑141 (MQ=255)
                                                                                                                                         tgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtatt     >  1:879824/1‑141 (MQ=255)
                                                                                                                                           tCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtattat   >  1:715924/1‑141 (MQ=255)
                                                                                                                                           tCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtattat   <  1:224336/141‑1 (MQ=255)
                                                                                                                                           tCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtattat   <  2:812477/141‑1 (MQ=255)
                                                                                                                                            cGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTattatt  <  1:455736/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TTCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTATTATT  >  CP000730/631969‑632249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: