BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	668,096	G→A	5.2%	A27A (GCG→GCA)	adh →	alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	668,096	0	G	A	5.2%	361.8 / 5.9	114	A27A (GCG→GCA)	adh	alcohol dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (54/54); new base A (4/2); total (58/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 114 total aligned reads displayed.

AGTAATAGCCAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGAATCAT  >  CP000730/667961‑668235

aGTAATAGCCAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGt                                                                                                                                        <  1:781017/141‑1 (MQ=255)

     tcgCCAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGAAAAAAAAAAAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCATTGGTACaaa                                                                                                                                   <  2:71024/139‑1 (MQ=255)

     tAGCCAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACaaa                                                                                                                                   <  1:837314/141‑1 (MQ=255)

         cAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGa                                                                                                                               <  1:309922/141‑1 (MQ=255)

         cAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGa                                                                                                                               <  1:309948/141‑1 (MQ=255)

           aTTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAat                                                                                                                             >  2:1086361/1‑141 (MQ=255)

           aTTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTGTAAAACCTGGTGAAGCGTTGGTACAAACGGAat                                                                                                                             >  1:754048/1‑141 (MQ=255)

            ttAATGTCATACATACTGGAGTGATGGTAAGTAGTACATGAGTAGTAGAGCAACAGTTGTAACGAAAGAACACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAata                                                                                                                            <  1:762571/141‑1 (MQ=255)

            ttAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAata                                                                                                                            <  2:781665/141‑1 (MQ=255)

             tAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAatat                                                                                                                           >  1:67269/1‑141 (MQ=255)

             tAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTAGTGAGGACAAAAAGTTAAGAGCTTTAAATCCTGGTGAAGCGTTGGTACAAACGGAatat                                                                                                                           <  2:1073846/141‑1 (MQ=255)

              aaTATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATAtt                                                                                                                          >  2:331253/1‑141 (MQ=255)

               atatCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATtg                                                                                                                         <  2:170694/141‑1 (MQ=255)

                tataATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATAACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACATGGTGAAGCGTTGGTACAAACGGAATATtgt                                                                                                                        >  2:470756/1‑141 (MQ=255)

                 atCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCATTGGTACAAACGGAATATtgtg                                                                                                                       >  2:110619/1‑141 (MQ=255)

                   catacataCTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGc                                                                                                                     >  1:186150/1‑141 (MQ=255)

                    atacatacTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCg                                                                                                                    >  1:962730/1‑141 (MQ=255)

                           cTGGAGCGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGAAATAAGAAAAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCa                                                                                                             <  2:1038712/141‑1 (MQ=255)

                             ggAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATa                                                                                                           >  2:898792/1‑141 (MQ=255)

                             ggAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATa                                                                                                           <  2:53805/141‑1 (MQ=255)

                              gAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATAc                                                                                                          >  2:135075/1‑141 (MQ=255)

                                 tGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGa                                                                                                       <  1:1726/141‑1 (MQ=255)

                                  gagtataAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGAt                                                                                                      <  2:959374/138‑1 (MQ=255)

                                    cTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGAttt                                                                                                    >  2:636560/1‑141 (MQ=255)

                                     tataAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTa                                                                                                   >  1:317692/1‑141 (MQ=255)

                                     tataAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTa                                                                                                   >  2:83894/1‑141 (MQ=255)

                                          ggaggaCATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGt                                                                                              >  2:94780/1‑141 (MQ=255)

                                              gaCATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAg                                                                                          >  2:868881/1‑141 (MQ=255)

                                              gaCATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAg                                                                                          >  1:1044287/1‑141 (MQ=255)

                                                cATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGaa                                                                                        >  1:746340/1‑141 (MQ=255)

                                                cATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGaa                                                                                        <  2:468724/141‑1 (MQ=255)

                                                   tattatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGc                                                                                     <  1:526452/141‑1 (MQ=255)

                                                   tattatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGc                                                                                     >  1:895775/1‑141 (MQ=255)

                                                   tattatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGc                                                                                     <  1:939582/141‑1 (MQ=255)

                                                   tattatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGGAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGc                                                                                     <  1:1078581/141‑1 (MQ=255)

                                                    attatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCt                                                                                    <  2:62342/141‑1 (MQ=255)

                                                    attatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCt                                                                                    >  1:1047237/1‑141 (MQ=255)

                                                    attatGAGAGCAGCAGTTGTAAAGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCt                                                                                    <  1:579959/141‑1 (MQ=255)

                                                      tatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGa                                                                                  >  1:206255/1‑141 (MQ=255)

                                                      tatGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGa                                                                                  >  2:107461/1‑141 (MQ=255)

                                                       atGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGAt                                                                                 >  1:984263/1‑141 (MQ=255)

                                                        tGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGAtt                                                                                <  1:110847/141‑1 (MQ=255)

                                                        tGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGAtt                                                                                >  1:948040/1‑141 (MQ=255)

                                                        tGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATGGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGAtt                                                                                >  2:652172/1‑141 (MQ=255)

                                                             gcagcaGTTGTAACGAAAGCTCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGt                                                                           <  2:646996/141‑1 (MQ=255)

                                                             gcagcaGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGt                                                                           <  2:558321/141‑1 (MQ=255)

                                                              cagcagTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTg                                                                          <  1:436577/141‑1 (MQ=255)

                                                                gcagTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCATTGGTACAAACGGAATATTGTGGTGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGAt                                                                        <  1:524077/141‑1 (MQ=255)

                                                                 cagTTGTAAGGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATg                                                                       >  1:956689/1‑141 (MQ=255)

                                                                  agTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGt                                                                      >  1:761656/1‑141 (MQ=255)

                                                                      gTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACa                                                                  >  1:576358/1‑141 (MQ=255)

                                                                       tAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAg                                                                 >  2:468147/1‑141 (MQ=255)

                                                                       tAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAg                                                                 >  2:176721/1‑141 (MQ=255)

                                                                          cGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTGCAGGCg                                                              >  2:293993/1‑141 (MQ=255)

                                                                          cGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCg                                                              <  2:307121/141‑1 (MQ=255)

                                                                          cGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCATTGGTACAAACGGAATATTGTGGTGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCg                                                              >  1:553636/1‑141 (MQ=255)

                                                                             aaGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTa                                                           <  1:863563/141‑1 (MQ=255)

                                                                             aaGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTa                                                           >  2:64447/1‑141 (MQ=255)

                                                                               gATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACt                                                         <  1:204126/141‑1 (MQ=255)

                                                                                  cacaAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTa                                                      >  2:749708/1‑141 (MQ=255)

                                                                                  cacaAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTa                                                      <  2:35732/141‑1 (MQ=255)

                                                                                      aagtaagtATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTc                                                  >  2:1012017/1‑141 (MQ=255)

                                                                                         taagtaTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATg                                               <  1:331253/141‑1 (MQ=255)

                                                                                         taagtaTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATg                                               <  1:1057009/141‑1 (MQ=255)

                                                                                          aagtaTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGa                                              <  2:640135/141‑1 (MQ=255)

                                                                                           agtaTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGTAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGaa                                             <  2:750102/141‑1 (MQ=255)

                                                                                           agtaTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGaa                                             >  1:648521/1‑141 (MQ=255)

                                                                                            gtaTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAg                                            <  1:460719/141‑1 (MQ=255)

                                                                                             taTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAgg                                           <  1:583007/141‑1 (MQ=255)

                                                                                             taTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAgg                                           >  1:1043783/1‑141 (MQ=255)

                                                                                              aTTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGt                                          <  2:451939/141‑1 (MQ=255)

                                                                                               ttGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTa                                         >  1:1058966/1‑141 (MQ=255)

                                                                                                  aGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTg                                      >  1:180585/1‑141 (MQ=255)

                                                                                                   ggACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTgg                                     <  2:506445/141‑1 (MQ=255)

                                                                                                   ggACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTgg                                     >  1:637691/1‑141 (MQ=255)

                                                                                                    gACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTCTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGt                                    <  2:945007/141‑1 (MQ=255)

                                                                                                     gataaaaGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTa                                   <  1:1020538/138‑1 (MQ=255)

                                                                                                         aaaGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGt                               <  1:337339/141‑1 (MQ=255)

                                                                                                         aaaGTTAAGAGCTTTAAAACCTGGTGAAGCATTGGTACAAACGGAATATTGTGGTGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGt                               >  1:305910/1‑141 (MQ=255)

                                                                                                          aaGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTc                              >  1:231968/1‑141 (MQ=255)

                                                                                                           aGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCa                             >  1:267433/1‑141 (MQ=255)

                                                                                                           aGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCa                             <  1:493416/141‑1 (MQ=255)

                                                                                                           aGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCa                             >  1:776609/1‑141 (MQ=255)

                                                                                                                agagCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGaa                        >  2:96348/1‑141 (MQ=255)

                                                                                                                agagCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGaa                        <  1:629369/141‑1 (MQ=255)

                                                                                                                agagCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGaa                        >  1:712469/1‑141 (MQ=255)

                                                                                                                   gCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATTCCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGtt                     <  1:342654/141‑1 (MQ=255)

                                                                                                                   gCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGtt                     <  1:529482/141‑1 (MQ=255)

                                                                                                                    cTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTg                    >  1:374258/1‑141 (MQ=255)

                                                                                                                     tttAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGc                   >  2:672295/1‑141 (MQ=255)

                                                                                                                        aaaaCCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGa                <  1:868881/141‑1 (MQ=255)

                                                                                                                          aaCCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGATAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAg              >  1:508114/1‑141 (MQ=255)

                                                                                                                           aCCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGa             <  2:746340/141‑1 (MQ=255)

                                                                                                                              tGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGt          >  2:258835/1‑141 (MQ=255)

                                                                                                                               ggTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTa         >  1:979423/1‑141 (MQ=255)

                                                                                                                                 tGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGa       <  2:906005/141‑1 (MQ=255)

                                                                                                                                     gCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGAATCa   <  1:43974/141‑1 (MQ=255)

                                                                                                                                     gCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGAATCa   <  1:468147/141‑1 (MQ=255)

                                                                                                                                      cGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGAATCAt  <  2:962730/141‑1 (MQ=255)

                                                                                                                                      cGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGAATCAt  >  1:76532/1‑141 (MQ=255)

AGTAATAGCCAATTAATATCATACATACTGGAGTGACTATAAGGAGGACATTATTATGAGAGCAGCAGTTGTAACGAAAGATCACAAAGTAAGTATTGAGGACAAAAAGTTAAGAGCTTTAAAACCTGGTGAAGCGTTGGTACAAACGGAATATTGTGGCGTTTGTCATACCGATTTACATGTTAAGAATGCTGATTTTGGTGATGTTACAGGCGTTACTTTAGGTCATGAAGGTATTGGTAAAGTCATCGAAGTTGCGGAAGATGTAGAATCAT  >  CP000730/667961‑668235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`