BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	25.3%	S213S (TCG→TCC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	25.3%	494.9 / 260.5	312	S213S (TCG→TCC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (95/138); new base G (34/45); total (129/183)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

Only 100 of 314 total aligned reads displayed.

TTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35774‑36053

ttGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCg                                                                                                                                              <  2:1069638/141‑1 (MQ=255)

        gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                                                      >  1:511351/1‑141 (MQ=255)

         agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                                                                     >  2:257420/1‑141 (MQ=255)

            taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                                                                                  <  2:745875/141‑1 (MQ=255)

                acaaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                                                            >  2:394234/15‑141 (MQ=11)

                acaaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                                                            >  2:782158/15‑141 (MQ=11)

                 taatatTGTTATCTAATATATATAGTATGTTCTGTTGCTAAGTAAAATTATATAGCTATCCACTTTTTTATTTTGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCTAAAATCCGTAGATCTGa                                                                                                                             <  2:197206/141‑1 (MQ=11)

                  aaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                                                            <  1:861822/141‑1 (MQ=255)

                        gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                    <  1:1020983/135‑1 (MQ=11)

                          gtccatataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                    <  2:588630/135‑1 (MQ=255)

                          attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                  <  2:1085775/137‑1 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 <  2:435037/138‑1 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 >  2:459019/4‑141 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 >  1:319195/4‑141 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 <  1:809773/138‑1 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 >  1:367765/4‑141 (MQ=11)

                             ttaataaAAAT‑GATGGTACTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                               >  2:763346/2‑141 (MQ=11)

                              taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                              <  1:597892/141‑1 (MQ=11)

                               aataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                               >  2:803895/1‑141 (MQ=39)

                               aataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTC‑t                                                                                                            >  2:319848/1‑141 (MQ=11)

                               aataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑t                                                                                                            >  2:661335/1‑141 (MQ=11)

                                    aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑tttt                                                                                                         >  1:741514/1‑141 (MQ=35)

                                     aaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑ttttt                                                                                                        <  1:986978/141‑1 (MQ=34)

                                     aaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑ttttt                                                                                                        >  1:28754/1‑141 (MQ=34)

                                                                          aatcACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTa                                                                   <  1:48336/138‑1 (MQ=1)

                                                                                             cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                >  1:942343/1‑141 (MQ=11)

                                                                                              aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAcg                                               <  1:1032149/141‑2 (MQ=11)

                                                                                               gtgtTNGTTTAACTTGCTAGCATGATGCTANTTTCGTGGCATGGGGAAAATCCGTNGATCTGNAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTANAGTATTCTTTGCCGTATTGATACtt                                              <  1:422656/141‑1 (MQ=11)

                                                                                               gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  1:569616/141‑1 (MQ=11)

                                                                                               gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              <  2:990110/141‑1 (MQ=12)

                                                                                               gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              >  2:125434/1‑141 (MQ=12)

                                                                                                   tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  1:660768/141‑1 (MQ=17)

                                                                                                   tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          >  2:645466/1‑141 (MQ=17)

                                                                                                   tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           >  2:1030157/1‑141 (MQ=12)

                                                                                                   tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  1:789860/141‑1 (MQ=17)

                                                                                                    cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  2:333448/4‑141 (MQ=17)

                                                                                                    cGCTTAACTTGCTAGCATGAAGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  2:141859/1‑141 (MQ=17)

                                                                                                     gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  1:242048/139‑1 (MQ=17)

                                                                                                     gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAACTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  1:214077/141‑1 (MQ=17)

                                                                                                      cTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:752757/141‑1 (MQ=17)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                      >  2:452715/1‑138 (MQ=11)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                      <  2:543445/141‑4 (MQ=255)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  1:220657/141‑1 (MQ=17)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  2:164020/141‑1 (MQ=17)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  1:644794/141‑1 (MQ=17)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      >  2:1056325/1‑141 (MQ=17)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  2:1041935/141‑1 (MQ=17)

                                                                                                        tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                     >  2:717240/1‑141 (MQ=11)

                                                                                                        tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                     >  1:116586/1‑141 (MQ=17)

                                                                                                        tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                     <  1:400824/141‑1 (MQ=17)

                                                                                                        tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                     <  1:151755/141‑1 (MQ=17)

                                                                                                         aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                    >  2:294770/1‑141 (MQ=11)

                                                                                                         aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                    >  1:996240/1‑141 (MQ=17)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  >  2:428411/1‑141 (MQ=32)

                                                                                                            ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:619871/141‑1 (MQ=32)

                                                                                                            ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:517697/141‑1 (MQ=17)

                                                                                                            ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:788686/141‑1 (MQ=17)

                                                                                                              gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                               >  2:507113/1‑141 (MQ=11)

                                                                                                              gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                               >  1:763171/1‑141 (MQ=255)

                                                                                                              gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               >  1:121911/1‑141 (MQ=17)

                                                                                                               cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTAAGGGTC‑TTTTTATATAGAGCGTAAATACATTCAATACC‑TTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  1:1052806/141‑1 (MQ=11)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             >  1:618709/1‑141 (MQ=11)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  2:83000/141‑1 (MQ=17)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  2:91391/141‑1 (MQ=17)

                                                                                                                  gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                           >  2:827079/1‑141 (MQ=11)

                                                                                                                    atgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                         <  2:891294/141‑1 (MQ=11)

                                                                                                                    atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                         <  2:75976/141‑1 (MQ=34)

                                                                                                                     tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  1:443801/1‑141 (MQ=255)

                                                                                                                     tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATCCAATACCTTTTGAAATATTCTTTGCCGTATTGATACATTGATCTCTTGtctattttcttc                        >  2:928727/1‑132 (MQ=11)

                                                                                                                     tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                        >  1:586225/1‑141 (MQ=21)

                                                                                                                      aatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                       <  2:417400/140‑1 (MQ=25)

                                                                                                                       atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                      <  2:153737/141‑1 (MQ=35)

                                                                                                                         gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                    <  2:279591/141‑1 (MQ=11)

                                                                                                                         gCTAATTTCGTGGCATGGCGAAAATCCGTAGAGCTGAAGAGACCTGCGGCTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTCTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                    >  1:325956/1‑141 (MQ=255)

                                                                                                                            aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 >  1:878443/1‑141 (MQ=25)

                                                                                                                            aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 <  2:841960/141‑1 (MQ=25)

                                                                                                                              tttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAc               >  2:2152/1‑141 (MQ=255)

                                                                                                                              tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc               <  1:563836/141‑1 (MQ=25)

                                                                                                                               ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              >  1:728267/1‑141 (MQ=255)

                                                                                                                               ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg              <  2:872074/141‑1 (MQ=37)

                                                                                                                                tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg             <  1:462099/141‑1 (MQ=255)

                                                                                                                                tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             >  2:901359/1‑141 (MQ=38)

                                                                                                                                 cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt            <  2:602033/141‑1 (MQ=255)

                                                                                                                                 cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt            <  2:947572/141‑1 (MQ=14)

                                                                                                                                 cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt            >  2:479391/1‑141 (MQ=14)

                                                                                                                                  gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg           <  2:351603/141‑1 (MQ=14)

                                                                                                                                  gTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTGCGGTTC‑TTTTTATATAGCGCGTACATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:918054/141‑1 (MQ=255)

                                                                                                                                   tGGCATGGCGAATTTACAGAGTGCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          <  1:603629/141‑1 (MQ=14)

                                                                                                                                   tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          >  1:679968/1‑141 (MQ=255)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:951619/1‑141 (MQ=39)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:26026/1‑141 (MQ=38)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:1085258/1‑141 (MQ=255)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:499991/1‑141 (MQ=255)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:663396/1‑141 (MQ=37)

                                                                                                                                     gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        >  1:1075356/1‑141 (MQ=39)

                                                                                                                                     gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCCTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  1:700357/141‑1 (MQ=255)

                                                                                                                                      cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt       >  2:264649/1‑141 (MQ=255)

                                                                                                                                       aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATGCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  2:675372/141‑1 (MQ=14)

                                                                                                                                       aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  1:85010/141‑1 (MQ=255)

                                                                                                                                       aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  2:466166/141‑1 (MQ=255)

TTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35774‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`