Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 696,863 T→C 5.2% V277V (GTT→GTC mnhA1 → CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730696,8630TC 5.2% 264.7 / 2.8 90V277V (GTT→GTCmnhA1CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit
Reads supporting (aligned to +/- strand):  ref base T (41/44);  new base C (3/2);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGGCTACCTAGAGCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGC  >  CP000730/696724‑697003
                                                                                                                                           |                                                                                                                                            
tttGGCTACCTAGAGCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTCAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCGCAGCAATATGTACGTATATATCGTTa                                                                                                                                             >  1:42479/1‑141 (MQ=255)
  tGGCTACCTAGAGCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACg                                                                                                                                           >  2:1086564/1‑141 (MQ=255)
    gCTACCTAGAGCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGtt                                                                                                                                         <  1:894797/141‑1 (MQ=255)
           agagCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGt                                                                                                                                  <  1:490672/141‑1 (MQ=255)
             agCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGttt                                                                                                                                <  1:111469/141‑1 (MQ=255)
               cAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTtaa                                                                                                                              >  1:115747/1‑141 (MQ=255)
                       gCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTtataatctgat                                                                                                                      >  1:664363/1‑132 (MQ=255)
                       gCACCCACACCTGTAAGTGCTTATCTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATg                                                                                                                      >  1:163246/1‑141 (MQ=255)
                        cACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGt                                                                                                                     <  2:721557/141‑1 (MQ=255)
                          ccTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTa                                                                                                                   >  1:491616/1‑141 (MQ=255)
                           cTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTAt                                                                                                                  <  1:469984/141‑1 (MQ=255)
                            tACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTAtt                                                                                                                 <  1:281378/141‑1 (MQ=255)
                             acacCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTAttt                                                                                                                >  2:943320/1‑141 (MQ=255)
                                ccTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAACCTGGTATCTTATTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATAGATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGt                                                                                                             >  2:652216/1‑141 (MQ=255)
                                 cTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGtt                                                                                                            >  2:766571/1‑141 (MQ=255)
                                   gTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTCTATATCGATACGTTGGTGGGTTTAATAACAATGGTATTTGGTTCa                                                                                                          >  1:209791/1‑141 (MQ=255)
                                   gTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCa                                                                                                          <  2:86881/141‑1 (MQ=255)
                                   gTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCa                                                                                                          <  2:86869/141‑1 (MQ=255)
                                    tAAGTGCTTATTTCCCTTCTGACAAGATTGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCaa                                                                                                         >  2:1087354/1‑141 (MQ=255)
                                    tAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCaa                                                                                                         <  2:311131/141‑1 (MQ=255)
                                         gCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTAAACCATTATAAGGCATTAGCAATATGTACGTACCAATTGTTCGGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACa                                                                                                    >  1:158144/1‑141 (MQ=255)
                                          cTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAg                                                                                                   <  1:226504/141‑1 (MQ=255)
                                          cTTAGTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAg                                                                                                   <  1:226120/141‑1 (MQ=255)
                                            tATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACAATAACTTTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCt                                                                                                 <  1:293980/141‑1 (MQ=255)
                                             aTTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCtt                                                                                                <  2:14133/141‑1 (MQ=255)
                                             aTTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCtt                                                                                                <  1:264022/141‑1 (MQ=255)
                                              tttACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCttt                                                                                               <  1:407559/141‑1 (MQ=255)
                                               gcgggtcggaacACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTa                                                                                              <  1:937847/130‑1 (MQ=255)
                                                tACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTaa                                                                                             <  1:537125/141‑1 (MQ=255)
                                                 aCATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTCGTTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTaaa                                                                                            <  1:19689/141‑1 (MQ=255)
                                                  cATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTaaaa                                                                                           >  2:335462/1‑141 (MQ=255)
                                                        gCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATgg                                                                                     <  1:22458/141‑1 (MQ=255)
                                                        gCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATgg                                                                                     >  2:659493/1‑141 (MQ=255)
                                                        gCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATgg                                                                                     <  1:242375/141‑1 (MQ=255)
                                                        gCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATgg                                                                                     >  1:712870/1‑141 (MQ=255)
                                                         ccACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATggg                                                                                    <  1:788839/141‑1 (MQ=255)
                                                         ccACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATggg                                                                                    <  2:456039/141‑1 (MQ=255)
                                                             gATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTAAAAGTTTAAAACAATGGGAttt                                                                                >  1:9552/1‑141 (MQ=255)
                                                              aTGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGATCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTa                                                                               <  2:957674/141‑1 (MQ=255)
                                                                   aaaaGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAgg                                                                          >  2:510983/1‑141 (MQ=255)
                                                                   aaaaGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAgg                                                                          >  2:510951/1‑141 (MQ=255)
                                                                    aaaGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGt                                                                         >  1:1061126/1‑141 (MQ=255)
                                                                    aaaGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGt                                                                         >  1:269464/1‑141 (MQ=255)
                                                                       gCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATc                                                                      <  1:503767/141‑1 (MQ=255)
                                                                         tGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCt                                                                    >  2:680981/1‑141 (MQ=255)
                                                                           gTATCTTTTTATTATTTTTATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAg                                                                  >  2:191034/1‑141 (MQ=255)
                                                                           gTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAg                                                                  >  2:641007/1‑141 (MQ=255)
                                                                            tATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGc                                                                 <  1:477966/141‑1 (MQ=255)
                                                                              tCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGt                                                               <  2:961719/141‑1 (MQ=255)
                                                                                 ttttATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAct                                                            <  1:52670/141‑1 (MQ=255)
                                                                                  tttATTACTTCTATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTActc                                                           >  2:596732/1‑141 (MQ=255)
                                                                                     attaCTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATTACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTAc                                                        >  1:1029732/1‑141 (MQ=255)
                                                                                           tCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAg                                                  <  1:841944/141‑1 (MQ=255)
                                                                                            cGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGt                                                 <  1:413776/141‑1 (MQ=255)
                                                                                             gATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTc                                                <  1:339341/141‑1 (MQ=255)
                                                                                                 tACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACt                                            >  2:483351/1‑141 (MQ=255)
                                                                                                   cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                          >  2:499583/1‑141 (MQ=255)
                                                                                                   cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                          >  2:763660/1‑141 (MQ=255)
                                                                                                   cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                          >  1:850539/1‑141 (MQ=255)
                                                                                                   cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                          >  2:718277/1‑141 (MQ=255)
                                                                                                      cATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGga                                       <  1:166746/141‑1 (MQ=255)
                                                                                                       attattAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGgat                                      >  2:477830/1‑141 (MQ=255)
                                                                                                         tattaGGTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGaatga                                    <  2:50171/141‑1 (MQ=255)
                                                                                                             aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                >  1:1046528/1‑141 (MQ=255)
                                                                                                             aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                <  1:87939/141‑1 (MQ=255)
                                                                                                             aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                >  1:1046372/1‑141 (MQ=255)
                                                                                                               gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                              >  1:212725/1‑141 (MQ=255)
                                                                                                               gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                              >  1:526260/1‑141 (MQ=255)
                                                                                                               gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                              >  2:406734/1‑141 (MQ=255)
                                                                                                               gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                              <  1:472694/141‑1 (MQ=255)
                                                                                                               gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAAAGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                              >  2:409041/1‑141 (MQ=255)
                                                                                                                      cAATATGTACGTATATATCGTTACGCTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGCTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggt                       >  2:949593/1‑141 (MQ=255)
                                                                                                                       aaTATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATAACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggta                      <  1:1028510/141‑2 (MQ=255)
                                                                                                                           tGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATTAGTCAACTTGGGATGATTATGGCTATGGTGGGta                  >  1:534463/1‑141 (MQ=255)
                                                                                                                           tGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATGGCAATGGTCGGta                  >  2:790719/1‑141 (MQ=255)
                                                                                                                             tACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGtata                >  1:366526/1‑141 (MQ=255)
                                                                                                                              aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg               <  2:688604/141‑1 (MQ=255)
                                                                                                                              aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg               <  2:792135/141‑1 (MQ=255)
                                                                                                                                gTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGt             >  2:66758/1‑141 (MQ=255)
                                                                                                                                 tatataTCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTg            <  2:269464/141‑1 (MQ=255)
                                                                                                                                  atatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTgg           <  2:850633/141‑1 (MQ=255)
                                                                                                                                   tatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAAGAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGc          <  1:661922/141‑1 (MQ=255)
                                                                                                                                      ttCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa       <  1:891516/140‑1 (MQ=255)
                                                                                                                                      atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa       <  1:183151/141‑1 (MQ=255)
                                                                                                                                      atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa       <  1:943320/141‑1 (MQ=255)
                                                                                                                                       tCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGat      <  2:240271/141‑1 (MQ=255)
                                                                                                                                       tCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTAAACTAGGAATGATTATGGCAATGGTCGGTATCGGTGGTGGat      >  1:204624/1‑141 (MQ=255)
                                                                                                                                        cGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGata     <  2:1040827/141‑1 (MQ=255)
                                                                                                                                         gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat    <  2:453683/141‑1 (MQ=255)
                                                                                                                                         gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat    >  1:406326/1‑141 (MQ=255)
                                                                                                                                          ttACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATg   <  2:302176/141‑1 (MQ=255)
                                                                                                                                           tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc  >  1:97988/1‑141 (MQ=255)
                                                                                                                                           tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc  >  1:661539/1‑141 (MQ=255)
                                                                                                                                           tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc  >  2:400823/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTTGGCTACCTAGAGCAATGGCTGCACCTACACCTGTAAGTGCTTATTTACATTCAGCCACGATGGTAAAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGC  >  CP000730/696724‑697003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: