Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 696,944 C→T 5.8% Y304Y (TAC→TAT mnhA1 → CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730696,9440CT 5.8% 258.6 / 2.8 84Y304Y (TAC→TATmnhA1CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit
Reads supporting (aligned to +/- strand):  ref base C (37/41);  new base T (3/2);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAATCATGCCA  >  CP000730/696805‑697083
                                                                                                                                           |                                                                                                                                           
ttttATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAct                                                                                                                                            <  1:52670/141‑1 (MQ=255)
 tttATTACTTCTATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTActc                                                                                                                                           >  2:596732/1‑141 (MQ=255)
    attaCTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATTACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTAc                                                                                                                                        >  1:1029732/1‑141 (MQ=255)
          tCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAg                                                                                                                                  <  1:841944/141‑1 (MQ=255)
           cGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGt                                                                                                                                 <  1:413776/141‑1 (MQ=255)
            gATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTc                                                                                                                                <  1:339341/141‑1 (MQ=255)
                tACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACt                                                                                                                            >  2:483351/1‑141 (MQ=255)
                  cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                          >  2:718277/1‑141 (MQ=255)
                  cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                          >  1:850539/1‑141 (MQ=255)
                  cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                          >  2:499583/1‑141 (MQ=255)
                  cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                                          >  2:763660/1‑141 (MQ=255)
                     cATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGga                                                                                                                       <  1:166746/141‑1 (MQ=255)
                      attattAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGgat                                                                                                                      >  2:477830/1‑141 (MQ=255)
                        tattaGGTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGaatga                                                                                                                    <  2:50171/141‑1 (MQ=255)
                            aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                                >  1:1046372/1‑141 (MQ=255)
                            aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                                <  1:87939/141‑1 (MQ=255)
                            aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                                >  1:1046528/1‑141 (MQ=255)
                              gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                              >  2:406734/1‑141 (MQ=255)
                              gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                              >  1:526260/1‑141 (MQ=255)
                              gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                              >  1:212725/1‑141 (MQ=255)
                              gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                                                                                                              <  1:472694/141‑1 (MQ=255)
                              gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAAAGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                                                                                                              >  2:409041/1‑141 (MQ=255)
                                     cAATATGTACGTATATATCGTTACGCTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGCTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggt                                                                                                       >  2:949593/1‑141 (MQ=255)
                                      aaTATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATAACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggta                                                                                                      <  1:1028510/141‑2 (MQ=255)
                                          tGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATTAGTCAACTTGGGATGATTATGGCTATGGTGGGta                                                                                                  >  1:534463/1‑141 (MQ=255)
                                          tGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATGGCAATGGTCGGta                                                                                                  >  2:790719/1‑141 (MQ=255)
                                            tACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGtata                                                                                                >  1:366526/1‑141 (MQ=255)
                                             aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg                                                                                               <  2:792135/141‑1 (MQ=255)
                                             aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg                                                                                               <  2:688604/141‑1 (MQ=255)
                                               gTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGt                                                                                             >  2:66758/1‑141 (MQ=255)
                                                tatataTCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTg                                                                                            <  2:269464/141‑1 (MQ=255)
                                                 atatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTgg                                                                                           <  2:850633/141‑1 (MQ=255)
                                                  tatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAAGAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGc                                                                                          <  1:661922/141‑1 (MQ=255)
                                                     ttCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                                       <  1:891516/140‑1 (MQ=255)
                                                     atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                                       <  1:183151/141‑1 (MQ=255)
                                                     atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                                       <  1:943320/141‑1 (MQ=255)
                                                      tCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGat                                                                                      <  2:240271/141‑1 (MQ=255)
                                                      tCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTAAACTAGGAATGATTATGGCAATGGTCGGTATCGGTGGTGGat                                                                                      >  1:204624/1‑141 (MQ=255)
                                                       cGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGata                                                                                     <  2:1040827/141‑1 (MQ=255)
                                                        gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat                                                                                    >  1:406326/1‑141 (MQ=255)
                                                        gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat                                                                                    <  2:453683/141‑1 (MQ=255)
                                                         ttACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATg                                                                                   <  2:302176/141‑1 (MQ=255)
                                                          tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                                  >  1:97988/1‑141 (MQ=255)
                                                          tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                                  >  2:400823/1‑141 (MQ=255)
                                                          tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                                  >  1:661539/1‑141 (MQ=255)
                                                             gTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCa                                                                               >  1:993416/1‑141 (MQ=255)
                                                              tttGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGATTTATAAAATTGGGATTTAATTGATGACTTAGTCTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCaa                                                                              <  1:647594/141‑1 (MQ=255)
                                                              taattgaaccaaataaCATTGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCaa                                                                              >  2:378934/12‑141 (MQ=255)
                                                               ttgttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAac                                                                             >  1:404146/1‑141 (MQ=255)
                                                                tgttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAaca                                                                            <  1:234458/141‑1 (MQ=255)
                                                                  ttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACAcc                                                                          <  1:596732/141‑1 (MQ=255)
                                                                       ttAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACcaacaa                                                                     <  1:972508/141‑1 (MQ=255)
                                                                        taataaCAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAg                                                                    >  1:688480/1‑141 (MQ=255)
                                                                            aaCAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGc                                                                >  1:537701/1‑141 (MQ=255)
                                                                               aaTGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAAt                                                             <  2:290607/141‑1 (MQ=255)
                                                                                  gTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGc                                                          <  2:163246/141‑1 (MQ=255)
                                                                                     aTTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATc                                                       >  1:46561/1‑141 (MQ=255)
                                                                                         ggTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTAtt                                                   <  1:842925/141‑1 (MQ=255)
                                                                                          gTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTAttt                                                  >  2:880435/1‑141 (MQ=255)
                                                                                            tCAATTCCAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAATCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAt                                                <  1:867402/141‑1 (MQ=255)
                                                                                            tCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAt                                                <  1:1084320/141‑1 (MQ=255)
                                                                                             caaatACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtg                                               <  2:285899/137‑1 (MQ=255)
                                                                                             cAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtg                                               >  2:804408/1‑141 (MQ=255)
                                                                                               aTTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtgta                                             >  2:245824/1‑141 (MQ=255)
                                                                                                     gCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtgtatttgta                                       >  1:705877/1‑141 (MQ=255)
                                                                                                         tAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGtattat                                   >  1:406223/1‑141 (MQ=255)
                                                                                                                 tGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcg                           <  1:1075130/141‑1 (MQ=255)
                                                                                                                 tGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcg                           <  2:86087/141‑1 (MQ=255)
                                                                                                                  gggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc                          <  2:177707/141‑1 (MQ=255)
                                                                                                                  gggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc                          <  1:628697/141‑1 (MQ=255)
                                                                                                                  gggATTTAAAAGGTAGCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACGCCAACATGGCGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc                          <  2:209791/141‑1 (MQ=255)
                                                                                                                   ggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCt                         <  2:32194/141‑1 (MQ=255)
                                                                                                                    gATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTa                        >  1:269434/1‑141 (MQ=255)
                                                                                                                     aTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTAt                       >  1:347279/1‑141 (MQ=255)
                                                                                                                     aTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTAt                       >  2:234547/1‑141 (MQ=255)
                                                                                                                        tAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTc                    <  1:378934/141‑1 (MQ=255)
                                                                                                                          aaaGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCAt                  <  2:178637/141‑1 (MQ=255)
                                                                                                                             ggTATCCTAGCGTACTCTACAATCAGTCAACTTGGCATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTa               >  1:948642/1‑141 (MQ=255)
                                                                                                                              gTATCCTAGCGTACTCTACAATCAGTCAACTTTGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTaa              <  1:60361/141‑1 (MQ=255)
                                                                                                                              gTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTaa              >  1:626406/1‑141 (MQ=255)
                                                                                                                               tATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAAt             <  2:491616/141‑1 (MQ=255)
                                                                                                                                   cTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAAt         >  1:281671/1‑141 (MQ=255)
                                                                                                                                         tACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAATCATGcc   <  1:718277/141‑1 (MQ=255)
                                                                                                                                         tACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAATCATGcc   <  1:483351/141‑1 (MQ=255)
                                                                                                                                         tACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAATCATGcc   <  2:473365/141‑1 (MQ=255)
                                                                                                                                          aCTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAATCATGCCa  <  1:210887/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAATGAATCATGCCA  >  CP000730/696805‑697083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: