Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 770,883 T→C 5.7% intergenic (+69/‑184) ebsC → / → fruR possible transcriptional regulator/DeoR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730770,8830TC 5.7% 287.2 / 4.9 100intergenic (+69/‑184)ebsC/fruRpossible transcriptional regulator/DeoR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (51/43);  new base C (2/4);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGAAAA  >  CP000730/770744‑771023
                                                                                                                                             |                                                                                                                                            
aaaTCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAGGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCAttt                                                                                                                                             >  1:852763/1‑141 (MQ=255)
 aaTCACAATAGCTGTTGAGGATTTGATTACAATAACAATAGGAATTATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTc                                                                                                                                            >  1:1062621/1‑141 (MQ=255)
      caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAAT‑‑TTCATCTCTgtca                                                                                                                                       <  1:513468/141‑4 (MQ=37)
      caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCca                                                                                                                                       <  1:343746/141‑1 (MQ=255)
      caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCca                                                                                                                                       >  1:940198/1‑141 (MQ=255)
      caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATCTCTGCca                                                                                                                                       >  2:50117/1‑141 (MQ=255)
       aaTAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCcac                                                                                                                                      >  2:565472/1‑141 (MQ=255)
        aTAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAAT‑‑TTCATCTCTGTcaca                                                                                                                                     <  1:89941/141‑1 (MQ=25)
         tAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAAT‑‑TTCATCTCTGTCacac                                                                                                                                    <  2:747753/141‑1 (MQ=25)
         tAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCacac                                                                                                                                    <  1:196588/141‑1 (MQ=255)
         tAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATACAAGTAGGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCacac                                                                                                                                    <  1:510434/141‑1 (MQ=255)
         tAGCTGGTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGGAGTTATGCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCNGCCacac                                                                                                                                    >  2:811893/1‑141 (MQ=255)
          aGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACt                                                                                                                                   >  2:312241/1‑141 (MQ=255)
          aGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACt                                                                                                                                   >  1:347299/1‑141 (MQ=255)
          aGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACt                                                                                                                                   <  2:945713/141‑1 (MQ=255)
           gCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTc                                                                                                                                  >  1:1012512/1‑141 (MQ=255)
                tGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCtttt                                                                                                                             <  2:320156/141‑1 (MQ=255)
                tGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCtttt                                                                                                                             <  1:421627/141‑1 (MQ=255)
                  aGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTttg                                                                                                                           <  1:712721/141‑1 (MQ=255)
                   ggATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTttga                                                                                                                          <  1:106296/141‑1 (MQ=255)
                    gATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGCGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTttgat                                                                                                                         <  2:304992/141‑1 (MQ=255)
                        tGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGaa                                                                                                                     >  1:668122/1‑141 (MQ=255)
                          aTTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAAtt                                                                                                                   >  1:598011/1‑141 (MQ=255)
                          aTTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAAtt                                                                                                                   <  2:253752/141‑1 (MQ=255)
                             aCAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGc                                                                                                                <  2:656335/141‑1 (MQ=255)
                             aCAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGc                                                                                                                >  2:623384/1‑141 (MQ=255)
                             aCAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGc                                                                                                                >  1:55545/1‑141 (MQ=255)
                                   aCTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTa                                                                                                          >  2:82466/1‑141 (MQ=255)
                                    cTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTAc                                                                                                         >  2:295342/1‑141 (MQ=255)
                                     tAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACg                                                                                                        >  1:1074941/1‑141 (MQ=255)
                                       aaGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGAt                                                                                                      <  2:794501/141‑1 (MQ=255)
                                       aaGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGAt                                                                                                      >  2:1042360/1‑141 (MQ=255)
                                         ggCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCa                                                                                                    >  2:361457/1‑141 (MQ=255)
                                                ttGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAg                                                                                             >  2:799818/1‑141 (MQ=255)
                                                  gtaGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTc                                                                                           <  1:596126/139‑1 (MQ=255)
                                                     gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                                                        >  2:95295/1‑141 (MQ=255)
                                                     gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                                                        >  2:550963/1‑141 (MQ=255)
                                                     gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                                                        >  1:878744/1‑141 (MQ=255)
                                                        gTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATtata                                                                                     <  2:229356/141‑1 (MQ=255)
                                                          tATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAAt                                                                                   <  2:500683/141‑1 (MQ=255)
                                                             ctatgaatgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTTTTTTTTTTTTCATTTTACGATCATAAACAGTCATTATAATTgt                                                                                >  2:1029093/3‑140 (MQ=255)
                                                             ccATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGa                                                                                <  2:469242/141‑1 (MQ=255)
                                                                  aatgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTAtt                                                                           >  1:375621/1‑141 (MQ=255)
                                                                    tgaTTAATAACAACAAAGAGTATGGGGCGAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTg                                                                         <  1:698575/141‑1 (MQ=255)
                                                                    tgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTg                                                                         >  1:408867/1‑141 (MQ=255)
                                                                      aTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGaa                                                                       >  1:1063425/1‑141 (MQ=255)
                                                                       ttAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAAc                                                                      <  2:841748/141‑1 (MQ=255)
                                                                        taataaCAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTTATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACa                                                                     >  1:884997/1‑141 (MQ=255)
                                                                          ataaCAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATa                                                                   >  1:914219/1‑141 (MQ=255)
                                                                            aacaacAAAAAGTATGGGACAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATaaa                                                                 <  2:806168/141‑1 (MQ=255)
                                                                               aacaaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATaaaaag                                                              <  1:1031100/141‑2 (MQ=255)
                                                                               aacaaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAAt                                                              >  1:90338/1‑141 (MQ=255)
                                                                                 caaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGt                                                            <  1:1042360/141‑1 (MQ=255)
                                                                                   aaaaGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTaa                                                          >  1:481824/1‑141 (MQ=255)
                                                                                       gTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAtttt                                                      >  1:488047/1‑141 (MQ=255)
                                                                                       gTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAtttt                                                      >  1:983944/1‑141 (MQ=255)
                                                                                       gTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAtttt                                                      >  2:171733/1‑141 (MQ=255)
                                                                                        tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCCCACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGCGTATTTGAACATAAAAATGTAATTTTa                                                     >  1:641523/1‑141 (MQ=255)
                                                                                        tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTa                                                     >  2:465880/1‑141 (MQ=255)
                                                                                        tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTa                                                     >  2:398458/1‑141 (MQ=255)
                                                                                        tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTa                                                     >  2:398380/1‑141 (MQ=255)
                                                                                          tGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTAtt                                                   >  1:584683/1‑140 (MQ=255)
                                                                                           ttcaatcaaaaaGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCg                                                  >  1:118067/12‑141 (MQ=255)
                                                                                           ggggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCGTTTTTTTTTTTTTTGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATct                                                  >  2:1023401/1‑140 (MQ=255)
                                                                                            gggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGt                                                 >  2:800086/1‑141 (MQ=255)
                                                                                               cAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTcac                                              <  2:410165/141‑1 (MQ=255)
                                                                                                 aGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACaa                                            >  2:650550/1‑141 (MQ=255)
                                                                                                 aGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACaa                                            >  2:649693/1‑141 (MQ=255)
                                                                                                    ttAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAAttt                                         <  1:1080548/141‑1 (MQ=255)
                                                                                                      aGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTTTTTTTTTTTTTATTTTAAGATCATAAACAGTCATTATAATTGAGTATTTGAACATAACAATGTACTATTAACGTTTCAATTTGa                                       >  2:490981/1‑141 (MQ=255)
                                                                                                      aGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa                                       <  2:803734/141‑1 (MQ=255)
                                                                                                      aGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa                                       >  1:303094/1‑141 (MQ=255)
                                                                                                      aGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa                                       >  1:598211/1‑141 (MQ=255)
                                                                                                        gAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAgt                                     >  2:1045307/1‑141 (MQ=255)
                                                                                                         aGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAgtg                                    >  2:309659/1‑141 (MQ=255)
                                                                                                           tgtgACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAATCTGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGtt                                  <  2:1022349/141‑1 (MQ=255)
                                                                                                                cAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGTTTTTTTTTTCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGa                             >  2:480502/1‑141 (MQ=255)
                                                                                                                 agagATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGAt                            >  1:563154/1‑141 (MQ=255)
                                                                                                                 agagATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGAt                            <  2:442368/141‑1 (MQ=255)
                                                                                                                 agagATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGAt                            >  2:165056/1‑141 (MQ=255)
                                                                                                                    gaaatgaattttATAAAAATTTCATCTCTGTCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGAtt                           >  1:867806/9‑141 (MQ=38)
                                                                                                                         aaTTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTg                    <  1:981408/141‑1 (MQ=255)
                                                                                                                         aaTTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTg                    <  1:491530/141‑1 (MQ=255)
                                                                                                                           tttttATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGt                  <  2:347299/141‑1 (MQ=255)
                                                                                                                           tttttATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGt                  <  2:944965/141‑1 (MQ=255)
                                                                                                                            ttttATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTa                 <  1:382652/141‑1 (MQ=255)
                                                                                                                             tttATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTaa                >  1:764489/1‑141 (MQ=255)
                                                                                                                             tttATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTaa                <  1:224461/141‑1 (MQ=255)
                                                                                                                               tataAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAAtt              <  1:584472/141‑1 (MQ=255)
                                                                                                                                ttaAAA‑‑TTAATTTCAGCCATAGTCCTTTTTGTTAGAATTATCATTTTACGATCATTAACAGTCATTATAATTGAGTATTTGATCATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAAttt             <  2:324258/140‑1 (MQ=255)
                                                                                                                                atcaaa‑‑TTCATTTCTGCCACACTCCTGTTTGATTGAATTAGCATTTTACGATCATAAACAGACATTATACTTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGCGTGTTTGTGATTGTTTTTGGTAAttt             <  1:83537/138‑1 (MQ=255)
                                                                                                                                 taAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTa            <  1:979606/141‑1 (MQ=255)
                                                                                                                                         tCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTg      <  1:811381/141‑1 (MQ=255)
                                                                                                                                          cATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGa     <  2:68452/141‑1 (MQ=255)
                                                                                                                                          cATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGa     <  1:531810/141‑1 (MQ=255)
                                                                                                                                          cATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGa     <  1:457274/141‑1 (MQ=255)
                                                                                                                                          cATCTCTGTCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGa     <  2:867806/141‑1 (MQ=255)
                                                                                                                                           aTTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGaa    <  2:118067/141‑1 (MQ=255)
                                                                                                                                            tttCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGaaa   >  1:119458/1‑141 (MQ=255)
                                                                                                                                             ttCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGaaaa  <  1:774846/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
AAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAA‑‑TTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTTTGTGATTGTTTTTGGTAATTTATGATTGAAAA  >  CP000730/770744‑771023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: