BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	864,745	C→T	6.9%	L21L (CTA→TTA)	est →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	864,745	0	C	T	6.9%	317.5 / 8.5	110	L21L (CTA→TTA)	est	carboxylesterase
Reads supporting (aligned to +/- strand): ref base C (47/54); new base T (4/4); total (52/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 113 total aligned reads displayed.

TAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTT  >  CP000730/864609‑864885

tAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACa                                                                                                                                           <  1:815795/141‑1 (MQ=255)

 aaGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACAt                                                                                                                                          <  1:843060/141‑1 (MQ=255)

  aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                                         >  2:992629/1‑141 (MQ=255)

  aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                                         <  1:418746/141‑1 (MQ=255)

  aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                                         <  1:989627/141‑1 (MQ=255)

   gAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATgg                                                                                                                                        <  2:236759/141‑1 (MQ=255)

    aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGGCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                                                       >  1:303433/1‑141 (MQ=255)

    aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                                                       <  2:346955/141‑1 (MQ=255)

      tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTANATGGtt‑t                                                                                                                                    >  2:815829/1‑141 (MQ=255)

      tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                                                    <  1:141115/141‑1 (MQ=255)

      tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                                                    >  2:578806/1‑141 (MQ=255)

      tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                                                    <  1:182046/141‑1 (MQ=255)

       gTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑tt                                                                                                                                   <  2:1079332/141‑1 (MQ=255)

            aGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAgg                                                                                                                              <  2:388708/141‑1 (MQ=255)

                tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                                          <  1:1037242/141‑1 (MQ=255)

                tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                                          <  2:876587/141‑1 (MQ=255)

                 aaCAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTA‑ATATTTGTTACAGGCAAtt                                                                                                                         >  1:603808/1‑141 (MQ=255)

                    aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGTAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGt                                                                                                                      >  2:331368/1‑141 (MQ=255)

                    aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGt                                                                                                                      >  2:659412/1‑141 (MQ=255)

                       aaGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTg                                                                                                                   >  1:246712/1‑141 (MQ=255)

                         gCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGAt                                                                                                                 >  2:325608/1‑141 (MQ=255)

                          cTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTTTAAGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATg                                                                                                                >  2:1026289/1‑141 (MQ=255)

                           tATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGt                                                                                                               <  2:387898/141‑1 (MQ=255)

                             tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                                             >  2:842588/1‑141 (MQ=255)

                             tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                                             >  1:501095/1‑141 (MQ=255)

                             tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                                             >  1:325545/1‑141 (MQ=255)

                             tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                                             >  2:779756/1‑141 (MQ=255)

                             tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAATACATGGTT‑TTACAGGCAATTCGTCTGGTGTTc                                                                                                             >  1:325558/1‑141 (MQ=255)

                              gTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCg                                                                                                            <  2:863824/141‑1 (MQ=255)

                                   atatatAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAAt                                                                                                       <  1:1059044/141‑1 (MQ=255)

                                      tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                                    >  1:76335/1‑141 (MQ=255)

                                      tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                                    >  1:76351/1‑141 (MQ=255)

                                       ataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCGATTAgg                                                                                                   <  1:964397/141‑1 (MQ=255)

                                        taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                  >  2:845134/1‑141 (MQ=255)

                                        taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                  >  1:989647/1‑141 (MQ=255)

                                          gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCACAACCTTTATTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTAGTCTGATGTACGTCAATTAGGTCg                                                                                                >  2:159022/1‑141 (MQ=255)

                                          gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                >  2:192552/1‑141 (MQ=255)

                                          gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                                                >  2:78141/1‑141 (MQ=255)

                                          gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                                                >  2:78101/1‑141 (MQ=255)

                                             tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                             <  1:917331/141‑1 (MQ=255)

                                             tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                             <  2:33945/141‑1 (MQ=255)

                                             tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                             <  2:852982/141‑1 (MQ=255)

                                               gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtttt                                                                                           >  1:1011906/1‑141 (MQ=255)

                                                  aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                                        <  1:958346/141‑1 (MQ=255)

                                                  aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                                        <  1:443758/141‑1 (MQ=255)

                                                   aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       <  1:435208/141‑1 (MQ=255)

                                                        gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                  <  2:699559/141‑1 (MQ=255)

                                                        gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                  <  2:385920/141‑1 (MQ=255)

                                                            aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                                              <  2:391707/141‑1 (MQ=255)

                                                             gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGTTTGTTAAGTTTGCGTGTTTTTATTATATGGTT‑TTACTGGCAATTCGTCTGATGTTCGTTAATTACGTCTATTTTTACAAGAGAAATGt                                                                             >  2:74066/1‑141 (MQ=255)

                                                             gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGt                                                                             <  2:400166/141‑1 (MQ=255)

                                                               gAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACaaaaaaagagata                                                                           <  1:109092/141‑10 (MQ=255)

                                                                 gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                         <  2:382280/141‑1 (MQ=255)

                                                                 gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                         <  1:716063/141‑1 (MQ=255)

                                                                   gTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTGTTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                                                       >  2:319000/1‑141 (MQ=255)

                                                                        aTTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCata                                                                  <  2:974823/141‑1 (MQ=255)

                                                                          ttATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATg                                                                <  1:610850/141‑1 (MQ=255)

                                                                               cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                                           <  1:722474/141‑1 (MQ=255)

                                                                               cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTATAGTGTTATTACTACATGTTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAAAAAGGTTATACATCATATGCACCg                                                           <  2:11662/141‑1 (MQ=255)

                                                                                aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  2:104898/141‑1 (MQ=255)

                                                                                   tAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAat                                                       >  2:345063/1‑141 (MQ=255)

                                                                                     aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                     >  1:1075010/1‑141 (MQ=255)

                                                                                     aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                     >  1:440928/1‑141 (MQ=255)

                                                                                        ttACCAAAACCTTTCTTTTTTTTTTTTTTTTAACGTGCAGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGGTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGATATACATCATATGTACTGCAATATGaa                                                  >  2:488527/1‑141 (MQ=255)

                                                                                          aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                <  1:78141/141‑1 (MQ=255)

                                                                                          aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                <  1:78101/141‑1 (MQ=255)

                                                                                           ccAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTGGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGc                                               >  1:1083375/1‑141 (MQ=255)

                                                                                            cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  1:425089/141‑1 (MQ=255)

                                                                                             aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                             >  1:653824/1‑141 (MQ=255)

                                                                                             aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                             >  1:109040/1‑141 (MQ=255)

                                                                                              aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                            >  1:514729/1‑141 (MQ=255)

                                                                                               aaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                           <  2:1042963/141‑1 (MQ=255)

                                                                                                aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:351197/141‑1 (MQ=255)

                                                                                                aCCCTTCTTTTTTGAGGCAGGTAAACGTGCCGTGTTATTACTACGTGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  2:94109/141‑1 (MQ=255)

                                                                                                    ttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGca                                      <  1:254814/141‑1 (MQ=255)

                                                                                                     tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                     >  1:335442/1‑141 (MQ=255)

                                                                                                     tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                     <  1:672670/141‑1 (MQ=255)

                                                                                                          tttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCAccacca                                <  1:794246/141‑1 (MQ=255)

                                                                                                            tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa                              >  2:763046/1‑141 (MQ=255)

                                                                                                              aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                            >  2:153131/1‑141 (MQ=255)

                                                                                                              aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                            <  1:717283/141‑1 (MQ=255)

                                                                                                               ggAAGGTAAACGTCCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa                           <  1:177527/141‑1 (MQ=255)

                                                                                                                ttttttAAACGTGCCGTGTGATTACTACATGGTT‑TTACAGGGAATTCGTCTGATGGTGGTCAATTAGGTCGATTTTTATTAATGAAATGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCATCACCAGATGag                          >  2:582879/6‑140 (MQ=255)

                                                                                                                gAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                          <  1:89797/141‑1 (MQ=255)

                                                                                                                gAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                          <  1:89841/141‑1 (MQ=255)

                                                                                                                    gTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAc                      <  2:142713/141‑1 (MQ=255)

                                                                                                                      aaaCGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTg                    <  1:672945/141‑1 (MQ=255)

                                                                                                                       aaCGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGa                   <  2:404359/141‑1 (MQ=255)

                                                                                                                        aCGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGaa                  >  2:728196/1‑141 (MQ=255)

                                                                                                                         cGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                 >  1:180587/1‑141 (MQ=255)

                                                                                                                         cGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                 >  1:1026470/1‑141 (MQ=255)

                                                                                                                         cGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                 >  1:186789/1‑141 (MQ=255)

                                                                                                                                tgtTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTc          >  2:344917/1‑141 (MQ=255)

                                                                                                                                 gtTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTcc         <  1:16966/141‑1 (MQ=255)

                                                                                                                                  ttattaCTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCt        <  1:277740/141‑1 (MQ=255)

                                                                                                                                   tattaCTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTAAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCCCGCGGCACCACCAGATGAAATACTGAAATCTAGTCCtt       <  2:499654/141‑1 (MQ=255)

                                                                                                                                     ttaCTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTc     >  2:960032/1‑141 (MQ=255)

                                                                                                                                      tactacATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCg    >  1:869182/1‑141 (MQ=255)

                                                                                                                                        ctacATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt  <  1:159298/141‑1 (MQ=255)

                                                                                                                                        ctacATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt  <  1:196574/141‑1 (MQ=255)

TAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTT  >  CP000730/864609‑864885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`