Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 864,753 T→A 5.9% G23G (GGT→GGA est → carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730864,7530TA 5.9% 293.8 / 8.8 102G23G (GGT→GGAestcarboxylesterase
Reads supporting (aligned to +/- strand):  ref base T (45/51);  new base A (3/3);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 109 total aligned reads displayed.

AATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTT  >  CP000730/864613‑864890
                                                                                                                                            |                                                                                                                                          
aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGGCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                                                            >  1:303433/1‑141 (MQ=255)
aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                                                            <  2:346955/141‑1 (MQ=255)
  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTANATGGttt                                                                                                                                         >  2:815829/1‑141 (MQ=255)
  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                         >  2:578806/1‑141 (MQ=255)
  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                         <  1:182046/141‑1 (MQ=255)
  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                         <  1:141115/141‑1 (MQ=255)
   gTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtttt                                                                                                                                        <  2:1079332/141‑1 (MQ=255)
        aGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAgg                                                                                                                                   <  2:388708/141‑1 (MQ=255)
           ttAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGCGTTATTACTACATGGTTTTACAGGCaa                                                                                                                                <  1:732883/141‑1 (MQ=255)
            tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAAt                                                                                                                               <  2:876587/141‑1 (MQ=255)
            tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAAt                                                                                                                               <  1:568249/141‑1 (MQ=255)
            tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAAt                                                                                                                               <  1:1037242/141‑1 (MQ=255)
             aaCAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTAATATTTGTTACAGGCAAtt                                                                                                                              >  1:603808/1‑141 (MQ=255)
                aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGTAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGt                                                                                                                           >  2:331368/1‑141 (MQ=255)
                aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGt                                                                                                                           >  2:659412/1‑141 (MQ=255)
                   aaGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTg                                                                                                                        >  1:246712/1‑141 (MQ=255)
                     gCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGAt                                                                                                                      >  2:325608/1‑141 (MQ=255)
                      cTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTTTAAGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATg                                                                                                                     >  2:1026289/1‑141 (MQ=255)
                       tATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGt                                                                                                                    <  2:387898/141‑1 (MQ=255)
                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                  >  2:842588/1‑141 (MQ=255)
                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                  >  1:501095/1‑141 (MQ=255)
                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                  >  1:325545/1‑141 (MQ=255)
                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                  >  2:779756/1‑141 (MQ=255)
                          gTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCg                                                                                                                 <  2:863824/141‑1 (MQ=255)
                               atatatAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAAt                                                                                                            <  1:1059044/141‑1 (MQ=255)
                                  tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                                         >  1:76351/1‑141 (MQ=255)
                                  tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                                         >  1:76335/1‑141 (MQ=255)
                                   ataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCGATTAgg                                                                                                        <  1:964397/141‑1 (MQ=255)
                                    taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                       >  1:989647/1‑141 (MQ=255)
                                    taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                       >  2:845134/1‑141 (MQ=255)
                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCACAACCTTTATTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTAGTCTGATGTACGTCAATTAGGTCg                                                                                                     >  2:159022/1‑141 (MQ=255)
                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                     >  2:345925/1‑141 (MQ=255)
                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                     >  2:192552/1‑141 (MQ=255)
                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                                                     >  2:78101/1‑141 (MQ=255)
                                        cTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAt                                                                                                   <  2:846310/141‑1 (MQ=255)
                                         tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                                  <  2:33945/141‑1 (MQ=255)
                                         tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                                  <  2:852982/141‑1 (MQ=255)
                                         tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                                  <  1:917331/141‑1 (MQ=255)
                                           gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtttt                                                                                                >  1:1011906/1‑141 (MQ=255)
                                              aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                                             <  1:443758/141‑1 (MQ=255)
                                              aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                                             <  1:958346/141‑1 (MQ=255)
                                               aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTATTTTAATCGTAACGAGGTATTACTGCGTGGTTTTACAGGCAATTTGGTTGATGTTGGTGGTTTAGGTCGATTTTTaaa                                                                                            >  2:731571/1‑139 (MQ=255)
                                               aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                            <  1:435208/141‑1 (MQ=255)
                                                gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaa                                                                                           >  1:127617/1‑141 (MQ=255)
                                                    gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                       >  2:1015686/1‑141 (MQ=255)
                                                    gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                       <  2:385920/141‑1 (MQ=255)
                                                    gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                       <  2:699559/141‑1 (MQ=255)
                                                        aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                                                   <  2:391707/141‑1 (MQ=255)
                                                         gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGTTTGTTAAGTTTGCGTGTTTTTATTATATGGTTTTACTGGCAATTCGTCTGATGTTCGTTAATTACGTCTATTTTTACAAGAGAAATGt                                                                                  >  2:74066/1‑141 (MQ=255)
                                                         gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGt                                                                                  <  2:400166/141‑1 (MQ=255)
                                                           gAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACaaaaaaagagata                                                                                <  1:109092/141‑10 (MQ=255)
                                                             gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                              <  1:716063/141‑1 (MQ=255)
                                                             gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                              <  2:382280/141‑1 (MQ=255)
                                                               gTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTGTTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                                                            >  2:319000/1‑141 (MQ=255)
                                                                    aTTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCata                                                                       <  2:974823/141‑1 (MQ=255)
                                                                           cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                                                <  1:722474/141‑1 (MQ=255)
                                                                           cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTATAGTGTTATTACTACATGTTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAAAAAGGTTATACATCATATGCACCg                                                                <  2:11662/141‑1 (MQ=255)
                                                                            aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                               <  2:104898/141‑1 (MQ=255)
                                                                               tAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAat                                                            >  2:345063/1‑141 (MQ=255)
                                                                                 aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                          >  1:440928/1‑141 (MQ=255)
                                                                                 aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                          >  1:1075010/1‑141 (MQ=255)
                                                                                      aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                     <  1:78101/141‑1 (MQ=255)
                                                                                       ccAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTGGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGc                                                    >  1:1083375/1‑141 (MQ=255)
                                                                                        cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                                   <  1:425089/141‑1 (MQ=255)
                                                                                         aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                                  >  1:109040/1‑141 (MQ=255)
                                                                                         aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                                  >  1:653824/1‑141 (MQ=255)
                                                                                          aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCGATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                                 <  1:203537/141‑1 (MQ=255)
                                                                                          aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                                 >  1:514729/1‑141 (MQ=255)
                                                                                            aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                               <  1:351197/141‑1 (MQ=255)
                                                                                            aCCCTTCTTTTTTGAGGCAGGTAAACGTGCCGTGTTATTACTACGTGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                               <  2:94109/141‑1 (MQ=255)
                                                                                               tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGc                                            <  2:400712/141‑1 (MQ=255)
                                                                                                ttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGca                                           <  1:254814/141‑1 (MQ=255)
                                                                                                 tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                          <  1:672670/141‑1 (MQ=255)
                                                                                                 tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                          >  1:335442/1‑141 (MQ=255)
                                                                                                   ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                        >  1:410589/1‑141 (MQ=255)
                                                                                                      tttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCAccacca                                     <  1:794246/141‑1 (MQ=255)
                                                                                                        tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa                                   >  2:763046/1‑141 (MQ=255)
                                                                                                          aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                                 <  1:717283/141‑1 (MQ=255)
                                                                                                          aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                                 >  2:153131/1‑141 (MQ=255)
                                                                                                           ggAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa                                >  1:892535/1‑141 (MQ=255)
                                                                                                            ttttttAAACGTGCCGTGTGATTACTACATGGTTTTACAGGGAATTCGTCTGATGGTGGTCAATTAGGTCGATTTTTATTAATGAAATGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCATCACCAGATGag                               >  2:582879/6‑140 (MQ=255)
                                                                                                            gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                               <  1:89797/141‑1 (MQ=255)
                                                                                                            gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                               <  1:89841/141‑1 (MQ=255)
                                                                                                                gTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAc                           <  2:142713/141‑1 (MQ=255)
                                                                                                                  aaaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTg                         <  1:672945/141‑1 (MQ=255)
                                                                                                                   aaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGa                        <  2:404359/141‑1 (MQ=255)
                                                                                                                    aCGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGaa                       >  2:728196/1‑141 (MQ=255)
                                                                                                                     cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                      >  1:1026470/1‑141 (MQ=255)
                                                                                                                     cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                      >  1:180587/1‑141 (MQ=255)
                                                                                                                     cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                      >  1:186789/1‑141 (MQ=255)
                                                                                                                             gtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTcc              <  1:16966/141‑1 (MQ=255)
                                                                                                                              ttattaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCt             <  1:277740/141‑1 (MQ=255)
                                                                                                                               tattaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTAAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCCCGCGGCACCACCAGATGAAATACTGAAATCTAGTCCtt            <  2:499654/141‑1 (MQ=255)
                                                                                                                                 ttaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTc          >  2:960032/1‑141 (MQ=255)
                                                                                                                                  tactacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCg         >  1:869182/1‑141 (MQ=255)
                                                                                                                                    ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt       <  1:159298/141‑1 (MQ=255)
                                                                                                                                    ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt       <  1:196574/141‑1 (MQ=255)
                                                                                                                                      acATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTg     <  1:221603/141‑1 (MQ=255)
                                                                                                                                      acATGGTTTTACAGGCAAGTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTg     <  2:792921/141‑1 (MQ=255)
                                                                                                                                         tGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGtt  <  1:631514/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTT  >  CP000730/864613‑864890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: