Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 864,777 T→A 7.3% V31V (GTT→GTA est → carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730864,7770TA 7.3% 313.2 / 13.9 105V31V (GTT→GTAestcarboxylesterase
Reads supporting (aligned to +/- strand):  ref base T (44/53);  new base A (5/3);  total (49/56)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

Only 100 of 105 total aligned reads displayed.

TGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGAT  >  CP000730/864638‑864915
                                                                                                                                           |                                                                                                                                          
tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                                           >  2:779756/1‑141 (MQ=255)
tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                                           >  1:501095/1‑141 (MQ=255)
tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                                           >  1:325545/1‑141 (MQ=255)
tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTc                                                                                                                                           >  2:842588/1‑141 (MQ=255)
 gTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCg                                                                                                                                          <  2:863824/141‑1 (MQ=255)
      atatatAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAAt                                                                                                                                     <  1:1059044/141‑1 (MQ=255)
         tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                                                                  >  1:76335/1‑141 (MQ=255)
          ataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCGATTAgg                                                                                                                                 <  1:964397/141‑1 (MQ=255)
           taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                                                >  2:845134/1‑141 (MQ=255)
           taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                                                >  1:989647/1‑141 (MQ=255)
             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCACAACCTTTATTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTAGTCTGATGTACGTCAATTAGGTCg                                                                                                                              >  2:159022/1‑141 (MQ=255)
             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                                              >  2:345925/1‑141 (MQ=255)
             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                                              >  2:192552/1‑141 (MQ=255)
             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                                                                              >  2:78101/1‑141 (MQ=255)
             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                                                                              >  2:78141/1‑141 (MQ=255)
               cTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAt                                                                                                                            <  2:846310/141‑1 (MQ=255)
                tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                                                           <  2:33945/141‑1 (MQ=255)
                tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                                                           <  2:852982/141‑1 (MQ=255)
                tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                                                           <  1:917331/141‑1 (MQ=255)
                  gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtttt                                                                                                                         >  1:1011906/1‑141 (MQ=255)
                     aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                                                                      <  1:443758/141‑1 (MQ=255)
                     aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                                                                      <  1:958346/141‑1 (MQ=255)
                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTATTTTAATCGTAACGAGGTATTACTGCGTGGTTTTACAGGCAATTTGGTTGATGTTGGTGGTTTAGGTCGATTTTTaaa                                                                                                                     >  2:731571/1‑139 (MQ=255)
                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                                                     <  1:435208/141‑1 (MQ=255)
                       gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaa                                                                                                                    >  1:127617/1‑141 (MQ=255)
                           gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                                                >  2:1015686/1‑141 (MQ=255)
                           gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                                                <  2:699559/141‑1 (MQ=255)
                           gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                                                <  2:385920/141‑1 (MQ=255)
                               aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                                                                            <  2:391707/141‑1 (MQ=255)
                                gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGTTTGTTAAGTTTGCGTGTTTTTATTATATGGTTTTACTGGCAATTCGTCTGATGTTCGTTAATTACGTCTATTTTTACAAGAGAAATGt                                                                                                           >  2:74066/1‑141 (MQ=255)
                                gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGt                                                                                                           <  2:400166/141‑1 (MQ=255)
                                  gAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACaaaaaaagagata                                                                                                         <  1:109092/141‑10 (MQ=255)
                                    gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                                                       <  2:382280/141‑1 (MQ=255)
                                    gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                                                       <  1:716063/141‑1 (MQ=255)
                                      gTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTGTTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                                                                                     >  2:319000/1‑141 (MQ=255)
                                             ttATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATg                                                                                              <  1:610850/141‑1 (MQ=255)
                                                  cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                                                                         <  1:722474/141‑1 (MQ=255)
                                                  cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTATAGTGTTATTACTACATGTTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAAAAAGGTTATACATCATATGCACCg                                                                                         <  2:11662/141‑1 (MQ=255)
                                                   aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                                                        <  2:104898/141‑1 (MQ=255)
                                                      tAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAat                                                                                     >  2:345063/1‑141 (MQ=255)
                                                        aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                                                   >  1:1075010/1‑141 (MQ=255)
                                                        aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                                                   >  1:440928/1‑141 (MQ=255)
                                                             aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                                              <  1:78141/141‑1 (MQ=255)
                                                             aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                                              <  1:78101/141‑1 (MQ=255)
                                                              ccAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTGGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGc                                                                             >  1:1083375/1‑141 (MQ=255)
                                                               cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                                                            <  1:425089/141‑1 (MQ=255)
                                                                aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                                                           >  1:109040/1‑141 (MQ=255)
                                                                aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                                                           >  1:653824/1‑141 (MQ=255)
                                                                 aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCGATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                                                          <  1:203537/141‑1 (MQ=255)
                                                                 aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                                                          >  1:514729/1‑141 (MQ=255)
                                                                  aaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                                                         <  2:1042963/141‑1 (MQ=255)
                                                                   aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                                                        <  1:351197/141‑1 (MQ=255)
                                                                   aCCCTTCTTTTTTGAGGCAGGTAAACGTGCCGTGTTATTACTACGTGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                                                        <  2:94109/141‑1 (MQ=255)
                                                                      tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGc                                                                     <  2:400712/141‑1 (MQ=255)
                                                                       ttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGca                                                                    <  1:254814/141‑1 (MQ=255)
                                                                        tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                                                   >  1:335442/1‑141 (MQ=255)
                                                                        tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                                                   <  1:672670/141‑1 (MQ=255)
                                                                          ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                                                 >  1:410589/1‑141 (MQ=255)
                                                                             tttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCAccacca                                                              <  1:794246/141‑1 (MQ=255)
                                                                               tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa                                                            >  2:763046/1‑141 (MQ=255)
                                                                                 aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                                                          <  1:717283/141‑1 (MQ=255)
                                                                                  ggAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa                                                         >  1:892535/1‑141 (MQ=255)
                                                                                  ggAAGGTAAACGTCCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa                                                         <  1:177527/141‑1 (MQ=255)
                                                                                   ttttttAAACGTGCCGTGTGATTACTACATGGTTTTACAGGGAATTCGTCTGATGGTGGTCAATTAGGTCGATTTTTATTAATGAAATGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCATCACCAGATGag                                                        >  2:582879/6‑140 (MQ=255)
                                                                                   gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                                                        <  1:89841/141‑1 (MQ=255)
                                                                                   gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                                                        <  1:89797/141‑1 (MQ=255)
                                                                                       gTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAc                                                    <  2:142713/141‑1 (MQ=255)
                                                                                         aaaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTg                                                  <  1:672945/141‑1 (MQ=255)
                                                                                          aaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGa                                                 <  2:404359/141‑1 (MQ=255)
                                                                                           aCGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGaa                                                >  2:728196/1‑141 (MQ=255)
                                                                                            cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                                               >  1:186789/1‑141 (MQ=255)
                                                                                            cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                                               >  1:1026470/1‑141 (MQ=255)
                                                                                            cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                                               >  1:180587/1‑141 (MQ=255)
                                                                                                   tgtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTc                                        >  2:344917/1‑141 (MQ=255)
                                                                                                    gtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTcc                                       <  1:16966/141‑1 (MQ=255)
                                                                                                     ttattaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCt                                      <  1:277740/141‑1 (MQ=255)
                                                                                                      tattaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTAAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCCCGCGGCACCACCAGATGAAATACTGAAATCTAGTCCtt                                     <  2:499654/141‑1 (MQ=255)
                                                                                                        ttaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTc                                   >  2:960032/1‑141 (MQ=255)
                                                                                                         tactacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCg                                  >  1:869182/1‑141 (MQ=255)
                                                                                                           ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt                                <  1:159298/141‑1 (MQ=255)
                                                                                                           ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt                                <  1:196574/141‑1 (MQ=255)
                                                                                                             acATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTg                              <  1:221603/141‑1 (MQ=255)
                                                                                                             acATGGTTTTACAGGCAAGTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTg                              <  2:792921/141‑1 (MQ=255)
                                                                                                                tGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGtt                           <  1:631514/141‑1 (MQ=255)
                                                                                                                       aCAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGGTTGGTTTAAAGAt                    >  2:123836/1‑141 (MQ=255)
                                                                                                                          ggCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCg                 >  2:173053/1‑141 (MQ=255)
                                                                                                                          ggCAATCCGTCTGCTGTTCGTCAATTAGGTCGATTTTTACAAAAGAACGGTTATACATCATCTGCACCGCAATATGAAGGCCACGCGGCACCACCAGCTGACATACTGAAATCTAGTCCTCTCCTTTCGTTTAAAGATGCg                 >  2:373804/1‑141 (MQ=255)
                                                                                                                           gCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGt                >  1:41959/1‑141 (MQ=255)
                                                                                                                           gCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGt                <  1:842588/141‑1 (MQ=255)
                                                                                                                                  gTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATgg         >  2:96157/1‑141 (MQ=255)
                                                                                                                                  gTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATgg         <  2:180587/141‑1 (MQ=255)
                                                                                                                                   tCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGt        >  2:344925/1‑141 (MQ=255)
                                                                                                                                    cTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGtt       <  1:578806/141‑1 (MQ=255)
                                                                                                                                    cTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGtt       >  2:182555/1‑141 (MQ=255)
                                                                                                                                    cTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGtt       >  1:774261/1‑141 (MQ=255)
                                                                                                                                      gaggTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTAt     <  2:325558/138‑1 (MQ=255)
                                                                                                                                      gATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTAt     <  1:827544/141‑1 (MQ=255)
                                                                                                                                      gATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTAt     <  2:325545/141‑1 (MQ=255)
                                                                                                                                         gTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGAt  <  2:743215/141‑1 (MQ=255)
                                                                                                                                         gTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGAt  <  1:152730/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGAT  >  CP000730/864638‑864915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: