Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 937,158 T→A 5.3% intergenic (+65/‑1952) yumB → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730937,1580TA 5.3% 263.5 / 4.7 88intergenic (+65/‑1952)yumB/USA300HOU_0903NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (46/37);  new base A (3/2);  total (49/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAA  >  CP000730/937021‑937297
                                                                                                                                          |                                                                                                                                           
aaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCAc                                                                                                                                          >  1:524111/1‑141 (MQ=255)
aaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGacac                                                                                                                                          <  1:1075985/141‑1 (MQ=255)
 aaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACt                                                                                                                                         <  1:1008379/141‑1 (MQ=255)
  aaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTg                                                                                                                                        >  2:153970/1‑141 (MQ=255)
   aGTGATTGATACACGTGCGGTATTCAAAATAGGTGGTATCGGTTTAAAAATCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGa                                                                                                                                       <  2:651729/141‑1 (MQ=255)
    gTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGaa                                                                                                                                      >  1:840856/1‑141 (MQ=255)
      gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                    <  1:841687/141‑1 (MQ=255)
      gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                    >  2:779370/1‑141 (MQ=255)
        ttgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGc                                                                                                                                  >  2:497608/1‑141 (MQ=255)
        ttgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGc                                                                                                                                  <  1:1083044/141‑1 (MQ=255)
            tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAGTGGCTTTa                                                                                                                              >  2:634268/1‑141 (MQ=255)
            tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAGGGGCTTTa                                                                                                                              >  2:580770/1‑141 (MQ=255)
                cGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                          >  2:949608/1‑141 (MQ=255)
                cGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                          >  2:704566/1‑141 (MQ=255)
                   gCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATtgttgt                                                                                                                       <  2:619975/141‑1 (MQ=255)
                    cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                      <  1:118769/141‑1 (MQ=255)
                     ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                     <  1:688015/141‑1 (MQ=255)
                     ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                     <  2:133603/141‑1 (MQ=255)
                     ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACTTTAtaaatgaat                                                                                                                     <  1:433477/141‑9 (MQ=38)
                      gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                    <  1:502115/141‑1 (MQ=255)
                      gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                    <  1:252870/141‑1 (MQ=255)
                        atccAAAATCGGTGGTATCGGGCTAGCATTCAAAAAAGGTAAATTCTAGGCTCTAAATAAAGTTTCAGCTACAACAAAACCACTACCCTATTCAATTATACAGTCCTCCAGTGTCACAGAAGGGCCTCATTGTTGTACAAt                                                                                                                  >  1:810909/4‑141 (MQ=255)
                          tCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATGCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAttt                                                                                                                <  1:247407/141‑1 (MQ=255)
                          tCAAAAACGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCATTATGACTGAAGGGCTTTATTGTTGTACAAttt                                                                                                                <  1:524374/141‑1 (MQ=255)
                            aaaaTCGGTGGTATCGGGTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                              <  2:128582/141‑1 (MQ=255)
                              aaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAg                                                                                                            <  1:122687/141‑1 (MQ=255)
                                tCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTg                                                                                                          <  1:632059/141‑1 (MQ=255)
                                tCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTg                                                                                                          <  1:54804/141‑1 (MQ=255)
                                  ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                        >  1:1075732/1‑141 (MQ=255)
                                   gtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGt                                                                                                       >  2:945286/1‑141 (MQ=255)
                                      gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                    >  2:142962/1‑141 (MQ=255)
                                      gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                    <  1:69414/141‑1 (MQ=255)
                                      gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                    >  1:251468/1‑141 (MQ=255)
                                             tttAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCaa                                                                                             >  1:443527/1‑141 (MQ=255)
                                                    ttCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                                      <  1:308663/141‑1 (MQ=255)
                                                    ttCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                                      >  1:202383/1‑141 (MQ=255)
                                                       aaaaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAaggag                                                                                   <  2:793941/141‑1 (MQ=255)
                                                                aaaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTAt                                                                          >  1:710926/1‑141 (MQ=255)
                                                                 aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                         <  1:651599/141‑1 (MQ=255)
                                                                 aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                         >  1:311548/1‑141 (MQ=255)
                                                                 aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                         >  2:836199/1‑141 (MQ=255)
                                                                      tAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAttt                                                                    <  2:503052/141‑1 (MQ=255)
                                                                      tAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAttt                                                                    <  1:447851/141‑1 (MQ=255)
                                                                        ggCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTtta                                                                  >  1:586357/1‑141 (MQ=255)
                                                                        ggCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTtta                                                                  >  2:619698/1‑141 (MQ=255)
                                                                        ggCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTtta                                                                  >  1:838764/1‑141 (MQ=255)
                                                                         gCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaa                                                                 <  1:569153/141‑1 (MQ=255)
                                                                           tttAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaat                                                               >  2:748648/1‑141 (MQ=255)
                                                                            ttAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaatt                                                              >  1:418190/1‑141 (MQ=255)
                                                                                   aaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAac                                                       <  1:64280/141‑1 (MQ=255)
                                                                                    aGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAaca                                                      >  2:133610/1‑141 (MQ=255)
                                                                                      tttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                                    <  1:35211/141‑1 (MQ=255)
                                                                                        tCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACt                                                  <  1:1002740/141‑1 (MQ=255)
                                                                                        tCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACt                                                  >  1:777628/1‑141 (MQ=255)
                                                                                         cAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCGGAGTCACTGAAGGGCTTTATTGTTGAACAATTCGAAGGGAGTAACATATCAAACCAAAGGTGGTACATTATGAATATGAAATTAAAGAACCCACTa                                                 >  2:317858/1‑141 (MQ=255)
                                                                                         cAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTa                                                 >  1:810604/1‑141 (MQ=255)
                                                                                         cAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAGTATGAATTTTAAATTAAATAACACAATa                                                 >  2:779242/1‑141 (MQ=255)
                                                                                           gCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAg                                               >  2:107386/1‑141 (MQ=255)
                                                                                              aaaCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCaa                                            >  2:929488/1‑141 (MQ=255)
                                                                                                aaCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAAc                                           <  1:473900/141‑1 (MQ=255)
                                                                                                 acacATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACg                                          <  1:467235/141‑1 (MQ=255)
                                                                                                    caTAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaa                                       <  2:278092/141‑1 (MQ=255)
                                                                                                       aaaaTTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataa                                    <  1:390789/141‑1 (MQ=255)
                                                                                                         aattaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTACGCAACGaaataaat                                  >  2:687120/1‑141 (MQ=255)
                                                                                                             aaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACAt                              >  1:315115/1‑141 (MQ=255)
                                                                                                              aattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGCAATAAATACAtt                             >  1:981937/1‑141 (MQ=255)
                                                                                                              aattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACAtt                             >  2:203185/1‑141 (MQ=255)
                                                                                                                 tattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGat                          >  1:978344/1‑141 (MQ=255)
                                                                                                                    tCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGATACATTATGAATTTTAAATTAAATAACACACTAAGAAACAAAATAAATACATTGattaa                       >  2:506109/1‑140 (MQ=255)
                                                                                                                     cATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGattatt                      >  2:558906/1‑141 (MQ=255)
                                                                                                                     cATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGattatt                      >  2:872431/1‑141 (MQ=255)
                                                                                                                       tttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                    <  2:828285/141‑1 (MQ=255)
                                                                                                                       tttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGAAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                    >  1:601787/1‑141 (MQ=255)
                                                                                                                        ttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGt                   <  1:128193/141‑1 (MQ=255)
                                                                                                                          ataAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtat                 <  1:622432/141‑1 (MQ=255)
                                                                                                                          ataAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtat                 >  2:287715/1‑141 (MQ=255)
                                                                                                                           taAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtata                >  1:840854/1‑141 (MQ=255)
                                                                                                                            aaaGTGCTTCAGTGTCACTGAAGGGATTTATTTTTGTACAATTTGAAGTGTGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAc               <  2:822212/141‑1 (MQ=255)
                                                                                                                             aaGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc              <  1:663640/141‑1 (MQ=255)
                                                                                                                               gcccTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACCTTGATTATTGGtatgcgag            >  2:896742/3‑136 (MQ=255)
                                                                                                                               gcccTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            >  2:284797/3‑141 (MQ=255)
                                                                                                                               gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACAGACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            >  1:10237/1‑141 (MQ=255)
                                                                                                                               gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            <  2:750006/141‑1 (MQ=255)
                                                                                                                               gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            >  1:660052/1‑141 (MQ=255)
                                                                                                                                tCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGa           >  1:1087315/1‑141 (MQ=255)
                                                                                                                                  tttCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAAc         <  2:911408/140‑1 (MQ=255)
                                                                                                                                  cTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAAc         >  2:718308/1‑141 (MQ=255)
                                                                                                                                      aGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAttt     <  1:821446/141‑1 (MQ=255)
                                                                                                                                      aGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAttt     >  1:257931/1‑141 (MQ=255)
                                                                                                                                       gtgtCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTa    >  2:728696/1‑141 (MQ=255)
                                                                                                                                         ttCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTaaa  <  2:932264/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAA  >  CP000730/937021‑937297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: