Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 938,905 G→A 6.6% intergenic (+1812/‑205) yumB → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730938,9050GA 6.6% 267.3 / 4.9 87intergenic (+1812/‑205)yumB/USA300HOU_0903NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base G (43/37);  new base A (2/4);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATTGTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACAGGTGTTAA  >  CP000730/938772‑939044
                                                                                                                                     |                                                                                                                                           
cGCATTGTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTa                                                                                                                                      >  2:397272/1‑141 (MQ=255)
 gCATTGTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAg                                                                                                                                     >  1:1050503/1‑141 (MQ=255)
  cATTGTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGa                                                                                                                                    >  2:999216/1‑141 (MQ=255)
    ttGTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAAc                                                                                                                                  <  1:61669/141‑1 (MQ=255)
      gTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACtt                                                                                                                                >  2:247837/1‑141 (MQ=255)
      gTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACtt                                                                                                                                >  2:346538/1‑141 (MQ=255)
       tATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTa                                                                                                                               <  2:96830/141‑1 (MQ=255)
        aTCACGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTACTATGGCAAAAAAATATTATAAGCATATGTTCATTCATTCAACGACTACTAATATTGCATTTTAGAACTTAg                                                                                                                              <  1:647158/141‑1 (MQ=255)
         tCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAgg                                                                                                                             <  2:1005738/141‑1 (MQ=255)
          cAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGa                                                                                                                            <  2:901537/141‑1 (MQ=255)
           aGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTTCATTTTAGAACTTAGGAt                                                                                                                           >  2:208313/1‑141 (MQ=255)
           aGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGAt                                                                                                                           >  2:615443/1‑141 (MQ=255)
           aGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGAt                                                                                                                           >  2:933784/1‑141 (MQ=255)
           aGGCATTTTCAATGATGTAAGAAAATATAAATCGCATATCCTTGTGAGCCTAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAAAAGCTCCTAAAACTAAATCTTATCACTTAGGAt                                                                                                                           >  2:486268/1‑141 (MQ=255)
            ggCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGAtt                                                                                                                          <  2:166057/141‑1 (MQ=255)
                    cATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTgg                                                                                                                  >  1:791770/1‑141 (MQ=255)
                    cATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGAAAAAAAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTgg                                                                                                                  <  2:687780/141‑1 (MQ=255)
                     aTTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGATTAGCATTGCAAAAACTTATTATAATCATATGCTCATTCAGTCAACGACTACTAATATAACATTATAGAACTTAGGATTTTGATTGgt                                                                                                                 >  2:1009413/1‑141 (MQ=255)
                           gtAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCa                                                                                                           >  1:202691/1‑141 (MQ=255)
                            tAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCaa                                                                                                          <  1:1006114/141‑1 (MQ=255)
                            tAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTGAGGAGTTTGATTGGTGTTCCaa                                                                                                          >  2:541749/1‑141 (MQ=255)
                               gAAAATATAAAGCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGAAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCt                                                                                                       <  2:1048628/141‑1 (MQ=255)
                                aaaaTATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAGGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCtt                                                                                                      <  2:397177/141‑1 (MQ=255)
                                aaaaTATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCAAAGAAAAAAAATATTATAGGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCtt                                                                                                      <  2:426585/141‑1 (MQ=255)
                                 aaaTATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTg                                                                                                     <  1:473508/141‑1 (MQ=255)
                                  aaTATACATCGCATATCCATGTCAGCATAACTCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCCGTCCACGACTACTAACCTTGCATTTTAGAACCTAGGCTTTTGCCTGGTGTTCCAAGCTTGt                                                                                                    <  2:918900/141‑1 (MQ=255)
                                  aaTATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGt                                                                                                    <  2:871307/141‑1 (MQ=255)
                                  aaTATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGt                                                                                                    <  1:740734/141‑1 (MQ=255)
                                   atatAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTa                                                                                                   >  2:52186/1‑141 (MQ=255)
                                     ataAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTaaa                                                                                                 >  1:1041022/1‑141 (MQ=255)
                                     ataAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTaaa                                                                                                 >  2:544071/1‑141 (MQ=255)
                                     ataAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTaaa                                                                                                 >  2:840431/1‑141 (MQ=255)
                                     ataAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTaaa                                                                                                 >  2:963992/1‑141 (MQ=255)
                                     ataAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATAATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGAGTTCCAAGCTTGTaaa                                                                                                 >  2:342782/1‑141 (MQ=255)
                                      taAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTaaaa                                                                                                <  1:1019961/141‑1 (MQ=255)
                                       aaaTCGCATATCCTTGTGATCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAAt                                                                                               >  1:723255/1‑141 (MQ=255)
                                       aaaTCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAAt                                                                                               <  2:205225/141‑1 (MQ=255)
                                       aaaTCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAAt                                                                                               <  2:68355/141‑1 (MQ=255)
                                        aaTCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAAtt                                                                                              >  2:340231/1‑141 (MQ=255)
                                         aTCGCATATCCTTGTGAGCATAAATCAACTTGATTAGCATTGCAAAAACTTATTATAATCATATGCTCATTCAGTCAACGACTACTAATATAACATTATAGAACTTAGGATTTTGATTGGTGTTCCAGGCTTGTAAAATTg                                                                                             <  1:964131/141‑1 (MQ=255)
                                         aTCGCATATCCTTGTGAGCATAAATCAACTTGATTAGCATTGCAAAAACTTATTATAATCATATGCTCATTCAGTCAACGACTACTAATATAACATTATAGAACTTAGGATTTTGATTGGTGTTCCAGGCTTGTAAAATTg                                                                                             <  1:964142/141‑1 (MQ=255)
                                                atCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGtttaatt                                                                                      >  2:34274/1‑141 (MQ=255)
                                                   cTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGtttaatttaa                                                                                   >  1:161871/1‑141 (MQ=255)
                                                       tgAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGaa                                                                               >  1:1055325/1‑141 (MQ=255)
                                                               aaTCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATATTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTaaaa                                                                       <  2:219810/141‑1 (MQ=255)
                                                                  cAACTTGATTAGCATTGCAAAAACTTATTATAATCATATGCTCATTCAGTCAACGACTACTAATATAACATTATAGAACTTAGGATTTTGATTGGTGTTCCAGGCTTGTAAAATTGTTTAATTTAATGAAAGTGAAAatta                                                                    <  2:266301/141‑1 (MQ=255)
                                                                      ttGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTAtttt                                                                <  2:1019066/141‑1 (MQ=255)
                                                                        gACTAGCATTACAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaa                                                              <  2:1028870/141‑1 (MQ=255)
                                                                         aCTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaag                                                             >  2:569168/1‑141 (MQ=255)
                                                                          cTAGCATTACAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaaga                                                            >  1:384343/1‑141 (MQ=255)
                                                                             gCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaagaaag                                                         <  1:108881/141‑1 (MQ=255)
                                                                             gCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaagaaag                                                         >  1:281932/1‑141 (MQ=255)
                                                                             acATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaagaaag                                                         <  2:736707/140‑1 (MQ=255)
                                                                              cATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaagaaaga                                                        >  1:886323/1‑141 (MQ=255)
                                                                              cATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTaagaaaga                                                        >  1:272534/1‑141 (MQ=255)
                                                                               aTTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGAc                                                       >  2:1068783/1‑141 (MQ=255)
                                                                                ttGCAAAAAAATATTATAAGCATATGTTCAGTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACa                                                      >  2:975735/1‑141 (MQ=255)
                                                                                    aaaaaaaTATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTa                                                  >  2:807379/1‑141 (MQ=255)
                                                                                       aaaaTATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACt                                               >  2:331063/1‑141 (MQ=255)
                                                                                         aaTATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTaa                                             >  1:531527/1‑141 (MQ=255)
                                                                                             ttatAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTaaaaaa                                         >  1:344533/1‑141 (MQ=255)
                                                                                               ataAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATa                                       <  1:501460/141‑1 (MQ=255)
                                                                                               ataAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATa                                       <  1:801257/141‑1 (MQ=255)
                                                                                                  aGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAat                                                                      >  2:309787/1‑107 (MQ=255)
                                                                                                  aGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAat                                                                      <  1:309787/107‑1 (MQ=255)
                                                                                                    cATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGat                                  >  1:415814/1‑141 (MQ=255)
                                                                                                     atatGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGatt                                 <  1:930719/141‑1 (MQ=255)
                                                                                                        tGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGattatt                              <  1:163209/141‑1 (MQ=255)
                                                                                                        tGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGattatt                              >  2:253522/1‑141 (MQ=255)
                                                                                                          ttcattcaGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTgg                            <  2:118991/141‑1 (MQ=255)
                                                                                                          ttcattcaGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTgg                            <  2:797019/141‑1 (MQ=255)
                                                                                                           tcattcaGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAAGTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGt                           <  2:280611/141‑1 (MQ=255)
                                                                                                            cattcaGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGta                          >  2:591555/1‑141 (MQ=255)
                                                                                                            cattcaGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGta                          <  1:796376/141‑1 (MQ=255)
                                                                                                               tcaGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAg                       <  1:541391/141‑1 (MQ=255)
                                                                                                                   tCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTg                   >  1:734597/1‑141 (MQ=255)
                                                                                                                   tCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTg                   >  2:506477/1‑141 (MQ=255)
                                                                                                                        gACTACTAATATAACATTATAGAACTTAGGATTTTGATTGGTGTTCCAGGCTTGTAAAATTGTTTAATTTAATGAAAGTGAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATGGGTATAGTTTGCTAtt              <  1:1009413/141‑1 (MQ=255)
                                                                                                                          ctactaATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGa            >  1:249349/1‑141 (MQ=255)
                                                                                                                          ctactaATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGa            >  2:758617/1‑141 (MQ=255)
                                                                                                                           tactaATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGAc           <  1:835418/141‑1 (MQ=255)
                                                                                                                           tactaATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGAc           >  1:138472/1‑141 (MQ=255)
                                                                                                                            actaATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACa          >  1:710175/1‑141 (MQ=255)
                                                                                                                             ctaATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACAg         <  2:234862/141‑1 (MQ=255)
                                                                                                                              taATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACAgg        >  1:76832/1‑141 (MQ=255)
                                                                                                                                   ttGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACAGGTGTTa   <  1:46515/141‑1 (MQ=255)
                                                                                                                                    tGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACAGGTGTTaa  <  2:272534/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                           
CGCATTGTATCAGGCATTTTCATTGTTGTAAGAAAATATAAATCGCATATCCTTGTGAGCATAAATCAACTTGACTAGCATTGCAAAAAAATATTATAAGCATATGTTCATTCAGTCAACGACTACTAATATTGCATTTTAGAACTTAGGATTTTGATTGGTGTTCCAAGCTTGTAAAATTGTTTAATTTAATGAAAGCTAAAATTATTTTAAGAAAGACAATTAACTAAAAAATAAAGATTATTGGTATAGTTTGCTATTGACAGGTGTTAA  >  CP000730/938772‑939044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: