Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,088,197 G→A 7.1% intergenic (‑515/‑355) USA300HOU_1021 ← / → USA300HOU_1022 hypothetical membrane protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,088,1970GA 7.1% 214.8 / 2.9 74intergenic (‑515/‑355)USA300HOU_1021/USA300HOU_1022hypothetical membrane protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (40/28);  new base A (4/2);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTTATCAATCATACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCTTTTTTATCTTT  >  CP000730/1088057‑1088337
                                                                                                                                             |                                                                                                                                            
tACTTATCAATCATACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTaaggaagg                                                                                                                                              >  2:173082/1‑141 (MQ=255)
  cTTATCAATCATACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACTAAAAAGTTGTAaggaagga                                                                                                                                             >  1:478853/1‑141 (MQ=255)
  cTTATCAATCATACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGgag                                                                                                                                            >  1:567593/1‑141 (MQ=255)
   ttATCAATCATACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGtaga                                                                                                                                           >  2:2404/1‑141 (MQ=255)
      tcaatcaTACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAggg                                                                                                                                        >  1:89364/1‑141 (MQ=255)
       caatcaTACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGa                                                                                                                                       >  2:761619/1‑141 (MQ=255)
          tcaTACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATAc                                                                                                                                    <  1:700083/141‑1 (MQ=255)
           caTACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACa                                                                                                                                   >  1:365776/1‑141 (MQ=255)
            aTACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACAt                                                                                                                                  >  2:1056138/1‑141 (MQ=255)
              aCCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATgc                                                                                                                                <  1:113719/141‑1 (MQ=255)
               ccTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATgcg                                                                                                                               <  2:1082603/141‑1 (MQ=255)
                 tctTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGcc                                                                                                                             >  2:243199/1‑141 (MQ=255)
                 tctTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGcc                                                                                                                             >  1:242797/1‑141 (MQ=255)
                     tAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGc                                                                                                                         <  1:346309/141‑1 (MQ=255)
                        caacaGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGcaca                                                                                                                      <  1:454174/141‑1 (MQ=255)
                         aacaGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACAt                                                                                                                     >  2:912207/1‑141 (MQ=255)
                         aacaGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACAt                                                                                                                     >  2:912345/1‑141 (MQ=255)
                            aGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCa                                                                                                                  <  1:143238/141‑1 (MQ=255)
                             gTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCAt                                                                                                                 >  2:738951/1‑141 (MQ=255)
                                 agaaCCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATaaaa                                                                                                             >  2:689497/1‑141 (MQ=255)
                                  gaaCCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAAt                                                                                                            >  2:931915/1‑141 (MQ=255)
                                  gaaCCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCACCGAGCACATGCATAAAAt                                                                                                            >  2:227600/1‑141 (MQ=255)
                                  gaaCCCGTGCATAATGGCTTACGAACTATAGTATGTAGGGTAAAAGAGGGATACATGCGCCGAGCACATGCAAAAAAGCTCCTCACAACT‑AAAAGTTGTAAGGTAAAAGAGGGATACATGCTCCGAGCACATGCaaaaaag                                                                                                            >  1:933390/1‑140 (MQ=21)
                                    aCCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATcc                                                                                                          >  1:134190/1‑141 (MQ=255)
                                                  gCTTACAAATTATAGTTTATAAGTAAGAAGAAGGATACATGCGCCGAGCACATGCGTAAAAGCCCCTAACAACT‑AAAATTTCTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTaaaa                                                                                            <  2:306720/141‑1 (MQ=37)
                                                    ttACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTTACAACttttagt                                                                                          <  1:939103/141‑7 (MQ=255)
                                                       cGAATTATAGTTTATAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAAGGAAACATACGCCGAGCAAAAGCATAAAATCCCCTAACAACTAAAAGTCGt                                                                                       >  1:351469/1‑141 (MQ=25)
                                                       cGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGt                                                                                       >  1:464396/1‑141 (MQ=255)
                                                         aactaaaggttgtaAGGAAGAAGAGGGATACATGCACCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGTAAAAGAGGGATACACGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTaa                                                                                     <  2:617573/129‑1 (MQ=21)
                                                          aTTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAg                                                                                    <  1:746344/141‑1 (MQ=255)
                                                             ataGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAgggg                                                                                 >  1:704816/1‑141 (MQ=255)
                                                             ataGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGAACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAgggg                                                                                 >  1:784603/1‑141 (MQ=255)
                                                                 tttATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACAAGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGAttt                                                                             >  2:967804/1‑141 (MQ=255)
                                                                   tataAGGAAGAAGAGGGATACATGCGGCGAGGACATGCAAAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTaa                                                                           <  2:327642/141‑1 (MQ=255)
                                                                   tataAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTaa                                                                           <  1:55134/141‑1 (MQ=255)
                                                                     taAGGAAGAAGGGGGATACATGCGCGGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAaat                                                                         <  2:752970/141‑1 (MQ=255)
                                                                       aggtaaaagaGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACT‑AAAAGTTGTAAGGAAGAAGAGGAACACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAaatta                                                                       >  1:882583/7‑141 (MQ=25)
                                                                       aGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAaatta                                                                       >  2:382865/1‑141 (MQ=255)
                                                                       aGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAaatta                                                                       >  2:907303/1‑141 (MQ=255)
                                                                          aagaagAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAaattaatt                                                                    >  1:83491/1‑141 (MQ=255)
                                                                           agaagaGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAAttt                                                                   >  1:721079/1‑141 (MQ=255)
                                                                             aagaGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAg                                                                 <  1:881171/141‑1 (MQ=255)
                                                                             aagaCGGAAACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAg                                                                 <  1:281209/141‑1 (MQ=255)
                                                                              agagGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTatt                                                                >  2:645528/1‑139 (MQ=255)
                                                                                agGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAgtgt                                                              <  2:134190/141‑1 (MQ=255)
                                                                                 gggACACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTa                                                             >  2:694231/1‑141 (MQ=255)
                                                                                        aTGCGCGAAGCACATGCATAAAAGGCCCTAACAACT‑AAAATTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGa                                                      <  2:1048789/141‑1 (MQ=255)
                                                                                            gcCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATc                                                  <  1:818015/141‑1 (MQ=255)
                                                                                             ccGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCt                                                 <  2:210506/141‑1 (MQ=255)
                                                                                             ccGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCt                                                 <  1:296921/141‑1 (MQ=255)
                                                                                              cGAGCACATGCATAAAAGCCCCTATGAACT‑AAAGGTTGTAAGGCACAAGAGGAATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAATAGTTGTAAGGGGATTTAGATTAATTTAGTGTATCTTGGATATCtt                                                <  2:933390/141‑1 (MQ=25)
                                                                                                 gCACATGCATAAAAGCCCCTAACAACT‑TAAAGTAGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgtt                                             >  1:817074/1‑141 (MQ=255)
                                                                                                 gCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgtt                                             <  1:180545/141‑1 (MQ=255)
                                                                                                   acaTGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgtttt                                           <  2:818304/141‑1 (MQ=255)
                                                                                                    caTGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgttttg                                          >  1:688527/1‑141 (MQ=255)
                                                                                                    caTGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgttttg                                          >  1:5745/1‑141 (MQ=255)
                                                                                                    caTGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgttttg                                          >  1:688542/1‑141 (MQ=255)
                                                                                                     aTGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCttgttttgt                                         <  1:6075/141‑1 (MQ=255)
                                                                                                         aTAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCAGAGCACCTGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGa                                     >  2:791584/1‑141 (MQ=255)
                                                                                                          tAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGAt                                    <  2:299656/141‑1 (MQ=255)
                                                                                                           aaaaGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGAtt                                   >  1:115364/1‑141 (MQ=255)
                                                                                                             aaGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTaa                                 <  1:343058/141‑1 (MQ=255)
                                                                                                               gCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAata                               <  1:1048562/141‑1 (MQ=255)
                                                                                                               gCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAata                               >  1:693881/1‑141 (MQ=255)
                                                                                                                ccccTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAatat                              >  2:595895/1‑141 (MQ=255)
                                                                                                                   cTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATAtctt                           >  2:145056/1‑141 (MQ=255)
                                                                                                                      acaacT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTg                        >  1:604508/1‑141 (MQ=255)
                                                                                                                        aacT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGtt                      >  2:605712/1‑141 (MQ=255)
                                                                                                                          cT‑AAAAGTTGTAAGGAAGAAGAGGAACACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGtttt                    >  2:137708/1‑141 (MQ=255)
                                                                                                                           t‑AAAAGTTGTAAGGAAGGAGAGGGATACATTCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGttttt                   >  2:442182/1‑141 (MQ=255)
                                                                                                                           t‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGttttt                   <  2:198836/141‑1 (MQ=255)
                                                                                                                            ttttaGTTGTTAGGAAGAAGAGGAACACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGttttt                   >  2:266543/5‑141 (MQ=37)
                                                                                                                               aaGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTACAACCTCAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTttct                <  1:836205/141‑1 (MQ=255)
                                                                                                                                    gTAAGGAAGGAGAGGGATAGATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCttt           >  1:170407/1‑141 (MQ=255)
                                                                                                                                    gTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCttt           <  2:261230/141‑1 (MQ=255)
                                                                                                                                    gTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCttt           >  1:459215/1‑141 (MQ=255)
                                                                                                                                    gTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCttt           >  2:175069/1‑141 (MQ=255)
                                                                                                                                     tAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCtttt          >  2:295954/1‑141 (MQ=255)
                                                                                                                                       aggaaggaGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCtttttt        >  1:3451/1‑141 (MQ=255)
                                                                                                                                           aggaGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCTTTTTTATCt    >  1:727192/1‑141 (MQ=255)
                                                                                                                                            ggaGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCTTTTTTATCtt   <  1:453413/141‑1 (MQ=255)
                                                                                                                                             gagaGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCTTTTTTATCttt  >  1:1082705/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TACTTATCAATCATACCTCTTTAACAACAGTGAAGAACCCGTGCATAATGGCTTACGAATTATAGTTTATAAGGAAGAAGAGGGATACATGCGCCGAGCACATGCATAAAAGCCCCTAACAACT‑AAAAGTTGTAAGGAAGGAGAGGGATACATGCGCCGAGCACATGCATAAAATCCCCTAACAACTAAAAGTTGTAAGGGGATTTAAATTAATTTAGTGTATCTTGGATATCTTGTTTTGTTTGATTAATATCTTCTGTTTTTTCTTCTTTTTTATCTTT  >  CP000730/1088057‑1088337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑