Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,218,734 A→C 6.0% intergenic (+225/+38) USA300HOU_1145 → / ← fbpA possible 3‑demethylubiquinone‑9 3‑methyltransferase/fibrinogen‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,218,7340AC 6.0% 228.7 / 6.5 77intergenic (+225/+38)USA300HOU_1145/fbpApossible 3‑demethylubiquinone‑9 3‑methyltransferase/fibrinogen‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (38/33);  new base C (2/3);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

AGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATGGTT  >  CP000730/1218596‑1218872
                                                                                                                                          |                                                                                                                                           
aGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGactact                                                                                                                                           <  2:475379/141‑1 (MQ=255)
aGCATTTTAGTTTCACCCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGactact                                                                                                                                           <  1:486890/141‑1 (MQ=255)
   aTTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActttc                                                                                                                                        <  1:467574/141‑1 (MQ=255)
   aTTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTActttc                                                                                                                                        <  2:436891/141‑1 (MQ=255)
       tAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCtttt                                                                                                                                   <  1:403078/141‑1 (MQ=255)
         gTTTCACTCGTTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTtgt                                                                                                                                 >  1:957632/1‑141 (MQ=255)
          tttCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACAAAAGAAAGTAGTCctttcttatggtg                                                                                                                                <  1:950053/141‑7 (MQ=37)
             cACTCATTTTTAAAATCACAAATAAAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTGGTAATTAACGCGTTAAATAAGAAATAGACGCCTCACCATGCGGATTGACTTCTTTCTTTtgctttg                                                                                                                             <  2:331144/141‑6 (MQ=255)
             cACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGTAGTCCTTTCTTAtggtgag                                                                                                                             >  2:512678/1‑136 (MQ=38)
                tCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGgtgt                                                                                                                          <  2:263923/141‑1 (MQ=255)
                    ttttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGTAGTCCTTTCTTATGGTGAGGTGTTTa                                                                                                                       <  1:512678/141‑1 (MQ=38)
                     tttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTAtt                                                                                                                     <  1:1082121/141‑1 (MQ=255)
                      ttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTAttt                                                                                                                    <  2:966766/141‑1 (MQ=255)
                      ttAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTAttt                                                                                                                    <  1:690849/141‑1 (MQ=255)
                       tAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTg                                                                                                                   >  1:417153/1‑141 (MQ=255)
                       tAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTg                                                                                                                   >  2:898991/1‑141 (MQ=255)
                         aaaTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACCCGATACTTCGTTTCAATGAACGCCTTCAATAATAAATAAACACCTCACCATAAGCAAGGACTACTTTCCTTTGTGAGGTGCTTCTTTGTc                                                                                                                 >  1:490520/1‑141 (MQ=255)
                         aaaTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTc                                                                                                                 >  1:490346/1‑141 (MQ=255)
                           aTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGa                                                                                                               >  1:787930/1‑141 (MQ=255)
                           aTCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGa                                                                                                               >  1:191457/1‑141 (MQ=255)
                             tttttaGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAGCACGATACTTCGTTTCAATGCACGCATTATTTAATATATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTTTTTGTCGACa                                                                                                             >  2:840904/6‑141 (MQ=255)
                               caAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAAc                                                                                                           >  1:12854/1‑141 (MQ=255)
                                    aTAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGAt                                                                                                      >  1:299360/1‑141 (MQ=255)
                                     tAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGAtt                                                                                                     >  2:605128/1‑141 (MQ=255)
                                      aaTTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTa                                                                                                    >  2:699643/1‑141 (MQ=255)
                                       aTTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTAt                                                                                                   <  1:497336/141‑1 (MQ=255)
                                           gTAACTTAAATGATGATACGTTATTTAAAAACATGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGAtt                                                                                               >  1:609308/1‑141 (MQ=255)
                                              aCTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGtt                                                                                            <  1:1052210/141‑1 (MQ=255)
                                                  aaaTGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCAt                                                                                        >  2:172847/1‑141 (MQ=255)
                                                         gatACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGa                                                                                 >  1:7134/1‑141 (MQ=255)
                                                                tATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGCGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGtt                                                                          <  1:549178/141‑1 (MQ=255)
                                                                  tttAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACCACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCa                                                                        <  1:345013/141‑1 (MQ=255)
                                                                  tttAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCa                                                                        <  1:345001/141‑1 (MQ=255)
                                                                       aaaCACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATc                                                                   <  2:498664/141‑1 (MQ=255)
                                                                       aaaCACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATc                                                                   >  2:247612/1‑141 (MQ=255)
                                                                         acacGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAg                                                                 <  2:983649/141‑1 (MQ=255)
                                                                          cacGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCGCAAAAGAAAGTAGTCCTTTCTTATGGTGAGGTGTTTATTTGTCGACAACTTGATTATAATTGTTTCATTTTTTGAATAAGTTCATAATCAgg                                                                <  2:296676/141‑1 (MQ=25)
                                                                            cGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGACAGGACTCCTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATGGTTTCATTTTTTGAATAAGTTCATACTCAgtcg                                                              >  2:752447/1‑138 (MQ=255)
                                                                            cGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGgtg                                                              >  1:1020274/1‑141 (MQ=255)
                                                                               tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           <  1:11803/141‑1 (MQ=255)
                                                                               tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           <  1:898991/141‑1 (MQ=255)
                                                                               tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           >  2:6675/1‑141 (MQ=255)
                                                                               tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           >  1:972385/1‑141 (MQ=255)
                                                                               tACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAg                                                           <  2:1082219/141‑1 (MQ=255)
                                                                                   tCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATa                                                       >  2:220865/1‑141 (MQ=255)
                                                                                    cGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATAc                                                      <  2:364981/141‑1 (MQ=255)
                                                                                    cGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATAc                                                      <  1:925660/141‑1 (MQ=255)
                                                                                          aaTGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGtt                                                <  1:25775/141‑1 (MQ=255)
                                                                                           aTGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGttt                                               >  2:165322/1‑141 (MQ=255)
                                                                                             gAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGttttt                                             <  2:502121/141‑1 (MQ=255)
                                                                                               aCGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTg                                           >  1:797330/1‑141 (MQ=255)
                                                                                                  cATTAAATAATAAATAACCACCTCACCATAAGAAAGAACTACTTTCTTTGGTGAGGTGTTTATTTGTCGACAACTTGATTATGAGTGTTTCATTTTTTGAATAAGTTCATAATCAGGTTTAGCATACAAAGTTTTTTGAtt                                        <  2:535074/141‑1 (MQ=255)
                                                                                                  cATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAtt                                        <  1:1078256/141‑1 (MQ=255)
                                                                                                  cATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGAtt                                        <  1:1020932/141‑1 (MQ=255)
                                                                                                   aTTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTTAATAATTTTATAATCAGGTGTAGCATACAAAGTTTTTTGATTg                                       >  2:584137/1‑141 (MQ=255)
                                                                                                   aTTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTg                                       >  1:345057/1‑141 (MQ=255)
                                                                                                    ttAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGt                                      <  1:359457/141‑1 (MQ=255)
                                                                                                      aaataataaataaACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCa                                    >  1:433713/1‑141 (MQ=255)
                                                                                                       aataataaataaACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCat                                   >  1:360034/1‑141 (MQ=255)
                                                                                                          aataaataaACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATg                                >  1:569662/1‑141 (MQ=255)
                                                                                                            taaataaaCACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATTATTTCATAATCAGTTGTAGCATACAAAGTTTTTTGATTGTCATATGtt                              >  2:950151/1‑141 (MQ=255)
                                                                                                               ataaaCACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACa                           >  2:582626/1‑141 (MQ=255)
                                                                                                                taaaCACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACaa                          >  1:37217/1‑141 (MQ=255)
                                                                                                                     acCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTTAATATTTTCATAATCATGTGTAGCATACAAAGTTTTTTGATTTTTATATGTTACAAACCCa                     >  2:978113/1‑141 (MQ=255)
                                                                                                                     acCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCa                     >  2:565242/1‑141 (MQ=255)
                                                                                                                       cTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTTTATTTTTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAgg                   >  2:387159/1‑141 (MQ=255)
                                                                                                                           ccATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCttt               >  2:278832/1‑141 (MQ=255)
                                                                                                                            cATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTg              <  2:149359/141‑1 (MQ=255)
                                                                                                                                aGATAGGACTACATTCTTTTGTGATTTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCAcc          <  1:97993/141‑1 (MQ=255)
                                                                                                                                aGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCtttaaaca          >  1:163/1‑136 (MQ=255)
                                                                                                                                aGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCAcc          <  2:1040425/141‑1 (MQ=255)
                                                                                                                                 gAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCt         <  2:933193/141‑1 (MQ=255)
                                                                                                                                 gAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCt         <  1:827470/141‑1 (MQ=255)
                                                                                                                                 gAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCt         <  1:510008/141‑1 (MQ=255)
                                                                                                                                  aaaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTg        >  2:867119/1‑141 (MQ=255)
                                                                                                                                  aaaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTg        >  2:238366/1‑141 (MQ=255)
                                                                                                                                  aaaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTg        <  1:743703/141‑1 (MQ=255)
                                                                                                                                   aaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGa       <  1:749704/141‑1 (MQ=255)
                                                                                                                                   aaGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCAAACAAAGTTTTTTGATTTTCATATGTTACAAACCCAGGCTTTGCACCTGa       >  1:541790/1‑141 (MQ=255)
                                                                                                                                        ctactTTCTTTTGTGGGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATGGtt  <  2:759257/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGCATTTTAGTTTCACTCATTTTTAAAATCACAAAGATAATTTGTAACTTAAATGATGATACGTTATTTAAAAACACGATACTTCGTTTCAATGAACGCATTAAATAATAAATAAACACCTCACCATAAGAAAGGACTACTTTCTTTTGTGAGGTGTTTATTTGTCGACAACTTGATTATGATTGTTTCATTTTTTGAATAAGTTCATAATCAGGTGTAGCATACAAAGTTTTTTGATTGTCATATGTTACAAACCCAGGCTTTGCACCTGATGGTT  >  CP000730/1218596‑1218872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: