Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,331,236 T→A 6.9% S188T (TCT→ACT)  USA300HOU_1233 → possible S33 family lysophophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,331,2360TA 6.9% 246.5 / 6.7 83S188T (TCT→ACT) USA300HOU_1233possible S33 family lysophophospholipase
Reads supporting (aligned to +/- strand):  ref base T (41/35);  new base A (2/4);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGAAAATGTTAAATC  >  CP000730/1331100‑1331371
                                                                                                                                        |                                                                                                                                       
tttACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAg                                                                                                                                     <  2:709200/141‑1 (MQ=255)
 ttACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAAGCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGt                                                                                                                                    <  2:642501/141‑1 (MQ=255)
 ttACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTACTAga                                                                                                                                    <  1:285173/141‑2 (MQ=255)
  tACCAAGCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATCACGTACACAAAGTGATTGGATTACTAgaa                                                                                                                                   <  2:829310/141‑3 (MQ=255)
   aCCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTaa                                                                                                                                  >  1:339497/1‑141 (MQ=255)
    cctaccgcgataGTTTTACAACGGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATAAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACACAGTGATTGCATTTCTAGTAAt                                                                                                                                 <  2:856670/130‑1 (MQ=255)
    ccAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAAt                                                                                                                                 >  2:656328/1‑141 (MQ=255)
    ccAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTAAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAAt                                                                                                                                 <  2:622679/141‑1 (MQ=255)
      aaCCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTACTAGAAATcc                                                                                                                               <  1:708866/141‑1 (MQ=255)
                gtgtTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTa                                                                                                                     >  1:769520/1‑141 (MQ=255)
                gtgtTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTa                                                                                                                     >  2:870405/1‑141 (MQ=255)
                 tgtTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAg                                                                                                                    <  1:996235/141‑1 (MQ=255)
                      cAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAAc                                                                                                               >  1:637331/1‑141 (MQ=255)
                        aCTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACtt                                                                                                             >  1:361895/1‑141 (MQ=255)
                         cTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACttt                                                                                                            >  2:211012/1‑141 (MQ=255)
                            aTTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTttatt                                                                                                         >  2:655365/1‑141 (MQ=255)
                             ttACAAAAACTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTttatta                                                                                                        >  1:954724/1‑141 (MQ=255)
                              tACAAAAATTTATGGTGCTGAGTAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTaa                                                                                                       >  1:910438/1‑141 (MQ=255)
                                 aaaaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGa                                                                                                    >  2:256575/1‑141 (MQ=255)
                                 aaaaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGa                                                                                                    >  2:1086147/1‑141 (MQ=255)
                                 aaaaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGa                                                                                                    >  1:880828/1‑141 (MQ=255)
                                  aaaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGAt                                                                                                   >  1:11145/1‑141 (MQ=255)
                                  aaaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGAt                                                                                                   >  1:10953/1‑141 (MQ=255)
                                   aaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATc                                                                                                  <  2:1002947/141‑1 (MQ=255)
                                   aaaTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATc                                                                                                  >  2:596902/1‑141 (MQ=255)
                                     aTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCa                                                                                                <  1:883161/141‑1 (MQ=255)
                                     aTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCa                                                                                                <  1:315375/141‑1 (MQ=255)
                                     aTTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCa                                                                                                <  2:769520/141‑1 (MQ=255)
                                          tGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAg                                                                                           <  2:984849/141‑1 (MQ=255)
                                            gTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTg                                                                                         >  2:45050/1‑141 (MQ=255)
                                             tGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTgg                                                                                        <  1:432860/141‑1 (MQ=255)
                                             tGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTgg                                                                                        <  2:618208/141‑1 (MQ=255)
                                             tGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTgg                                                                                        <  1:993510/141‑1 (MQ=255)
                                             tGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTgg                                                                                        <  1:258966/141‑1 (MQ=255)
                                                 gagaAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTTGATAACTTTATTAAAGATCCATATAGTGGAttt                                                                                    <  1:563431/141‑1 (MQ=255)
                                                 gagaAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGAttt                                                                                    <  2:527385/141‑1 (MQ=255)
                                                    aaaCGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAAt                                                                                 <  1:697915/141‑1 (MQ=255)
                                                       cGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAAtgtg                                                                              <  2:940569/141‑1 (MQ=255)
                                                        gAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATgtgt                                                                             <  1:870405/141‑1 (MQ=255)
                                                          gTTGAACGGGTTAAGCAGGTAGTATTAAATAGGTGTAATAAAAAAATACGTCCATTACGTACACAAAGTGAGTGGATTACCAGTAATCCAATTGAAGTAGAGAACTTTATTGAAGATCCATATAGTGGATTTAATGTGTCa                                                                           >  2:443951/1‑141 (MQ=255)
                                                           ttGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCaa                                                                          <  1:589710/141‑1 (MQ=255)
                                                           ttGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCaa                                                                          >  2:759569/1‑141 (MQ=255)
                                                             gAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAAt                                                                        >  2:968392/1‑141 (MQ=255)
                                                             gAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAAt                                                                        >  1:357393/1‑141 (MQ=255)
                                                             aaaTGGGTTAACCAGTTAGTATCAAATAAAAAAAAAAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAAt                                                                        <  2:87523/140‑1 (MQ=255)
                                                              aaTGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATc                                                                       <  1:635574/141‑1 (MQ=255)
                                                                 gggTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAat                                                                    <  2:700451/141‑1 (MQ=255)
                                                                  ggTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAatt                                                                   >  2:8178/1‑141 (MQ=255)
                                                                      aaCCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAAttatta                                                               >  2:112718/1‑141 (MQ=255)
                                                                       aCCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATtattat                                                              >  2:945216/1‑141 (MQ=255)
                                                                        ccAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATtata                                                             >  1:781207/1‑141 (MQ=255)
                                                                           gTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCa                                                          <  2:567378/141‑1 (MQ=255)
                                                                           gTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCa                                                          >  1:670854/1‑141 (MQ=255)
                                                                              aGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAAc                                                       <  2:83070/141‑1 (MQ=255)
                                                                                  tCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGcc                                                   >  1:720609/1‑141 (MQ=255)
                                                                                    aaaTAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCta                                                 >  2:751198/1‑141 (MQ=255)
                                                                                    aaaTAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCta                                                 >  1:38963/1‑141 (MQ=255)
                                                                                       tAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCtatta                                              >  1:636074/1‑141 (MQ=255)
                                                                                         gTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATtata                                            >  1:541503/1‑141 (MQ=255)
                                                                                                  aaaaaaTACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCtacata                                   >  2:303911/1‑141 (MQ=255)
                                                                                                   aaaaaTACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCtacatac                                  <  2:696314/141‑1 (MQ=255)
                                                                                                    aaaaTACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCtacataca                                 <  1:146120/141‑1 (MQ=255)
                                                                                                    aaaaTACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCtacataca                                 >  1:220709/1‑141 (MQ=255)
                                                                                                    aaaaTACGTCCATTACGTACACAAAGTGATTGGAGTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATTAAATAGCCTATTATATGCtacatata                                 >  2:844901/1‑139 (MQ=255)
                                                                                                      aaTACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATc                               <  1:996988/141‑1 (MQ=255)
                                                                                                        tACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATcac                             <  2:786703/141‑1 (MQ=255)
                                                                                                        tACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAAAAAAATTAAAAAAACATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCtacatacaaaaa                             <  2:725765/141‑5 (MQ=255)
                                                                                                          cGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACaa                           >  2:242331/1‑141 (MQ=255)
                                                                                                          cGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAAATTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCtacataaaaaaaaa                           <  2:725672/141‑9 (MQ=255)
                                                                                                           gTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAAt                          <  1:231973/141‑1 (MQ=255)
                                                                                                              cATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTaa                       >  2:966295/1‑141 (MQ=255)
                                                                                                                 tACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTaaaaa                    >  1:704945/1‑141 (MQ=255)
                                                                                                                  aCGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTaaaaaa                   <  1:946510/141‑1 (MQ=255)
                                                                                                                     tACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAatat                >  1:12492/1‑141 (MQ=255)
                                                                                                                     tACACAAAGTGATTGGATTCCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAatat                >  2:495797/1‑141 (MQ=255)
                                                                                                                       cacaAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGa              >  1:81776/1‑141 (MQ=255)
                                                                                                                        acaAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGaa             <  2:670854/141‑1 (MQ=255)
                                                                                                                           aaGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCCACATACATCACAATTAAAAAATATGAAAAt          >  1:632071/1‑141 (MQ=255)
                                                                                                                                 ttGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGAAAATGTTaaa    >  1:104483/1‑141 (MQ=255)
                                                                                                                                   ggATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGAAAATGTTAAATc  <  2:435486/141‑1 (MQ=255)
                                                                                                                                   ggATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGAAAATGTTAAATc  >  2:68519/1‑141 (MQ=255)
                                                                                                                                   ggATTACTAGAAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCt                                        <  1:976313/103‑1 (MQ=255)
                                                                                                                                   ggATTACTAGAAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCt                                        >  2:976313/1‑103 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTTACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAACGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTACGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGATCCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTACATACATCACAATTAAAAAATATGAAAATGTTAAATC  >  CP000730/1331100‑1331371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: