BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,372,042	A→T	6.1%	V524V (GTA→GTT)	tkt →	transketolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,372,042	0	A	T	6.1%	285.5 / 5.5	95	V524V (GTA→GTT)	tkt	transketolase
Reads supporting (aligned to +/- strand): ref base A (39/49); new base T (2/4); total (42/53)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAG  >  CP000730/1371903‑1372178

tAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTAt                                                                                                                                          <  1:440298/141‑1 (MQ=255)

   cTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAATTGTATTTTTAATGGTTCGGCGACCACCGAGATCTACACCTACTTCA‑TAGTATTGACACGTCAAAACTTACCGGTATTAGa                                                                                                                                      <  2:122999/141‑1 (MQ=255)

      gATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATg                                                                                                                                    >  1:717816/1‑141 (MQ=255)

      gATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATg                                                                                                                                    <  1:925406/141‑1 (MQ=255)

       aTTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAGGTTGGCTGAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑t                                                                                                                                  <  2:818727/141‑1 (MQ=255)

       aTTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑t                                                                                                                                  <  2:104292/141‑1 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                             >  2:448220/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                             >  2:954608/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                             >  1:260197/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                             >  1:90826/1‑141 (MQ=255)

            gagCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCag                                                                                                                             >  1:175534/1‑141 (MQ=255)

             agCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCaga                                                                                                                            >  2:531360/1‑141 (MQ=255)

              gCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCagaa                                                                                                                           >  1:169113/1‑141 (MQ=255)

               ccATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCCGAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCagaag                                                                                                                          <  2:302577/141‑1 (MQ=255)

                  ttCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATg                                                                                                                       >  2:193743/1‑141 (MQ=255)

                   tCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGTGTAGCATGGGAAGTTGCCTTACAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGt                                                                                                                      <  2:668538/141‑1 (MQ=255)

                    ccAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTa                                                                                                                     <  2:379350/141‑1 (MQ=255)

                    ccAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTa                                                                                                                     >  1:167359/1‑141 (MQ=255)

                    ccAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTa                                                                                                                     >  2:418883/1‑141 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAAGAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAg                                                                                                                    <  1:801949/141‑1 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGTAg                                                                                                                    <  1:820201/141‑1 (MQ=255)

                     cAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAg                                                                                                                    >  1:332176/1‑141 (MQ=255)

                     cAAATATGAATGATATCCGTCCTGCAGATCGTAATGAAACAAGAGTAGAATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGTAg                                                                                                                    >  2:138502/1‑141 (MQ=255)

                      aaaTATTAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGCGTAGCATGGGAAGTTGCCTGAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGt                                                                                                                   <  2:1037601/141‑1 (MQ=255)

                      aaaTATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGt                                                                                                                   <  2:495427/141‑1 (MQ=255)

                      aaaTATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGAAGAGTCACCACTTACTGGT‑GTAGGTGTTACCAGAGGATGTAGt                                                                                                                   >  1:313874/1‑141 (MQ=255)

                       aaTATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGATACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGtt                                                                                                                  >  1:855527/1‑141 (MQ=255)

                       aaTATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGtt                                                                                                                  >  1:107043/1‑141 (MQ=255)

                       aaTATGAATGTTATCCGTCCGGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGtt                                                                                                                  <  2:846927/141‑1 (MQ=255)

                          atGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTgaa                                                                                                               >  2:226376/1‑141 (MQ=255)

                           tgtattttATCCGTTCTGCTGATGGCAATGAAACAAGAGTAGCAGTGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAATATGTAGTTgaag                                                                                                              <  2:972005/135‑1 (MQ=255)

                                gTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGc                                                                                                         <  2:496534/141‑1 (MQ=255)

                                    tCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTc                                                                                                     >  2:34650/1‑141 (MQ=255)

                                     ccGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCg                                                                                                    <  2:630443/141‑1 (MQ=255)

                                     ccGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCg                                                                                                    <  2:772524/141‑1 (MQ=255)

                                       gTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGaa                                                                                                  >  2:1002149/1‑141 (MQ=255)

                                               gATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGcc                                                                                          <  1:615401/141‑1 (MQ=255)

                                               gATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGcc                                                                                          >  1:805148/1‑141 (MQ=255)

                                                 tGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCta                                                                                        >  1:988281/1‑141 (MQ=255)

                                                 tGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCta                                                                                        >  2:172394/1‑141 (MQ=255)

                                                  ggTAATGAGAAAAGAGTAGCATGGGTAGTTGCCTTAGGATATGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGGTG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAGAGGTGCCttt                                                                                       <  2:116623/141‑3 (MQ=255)

                                                  ggTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCtat                                                                                       <  2:116688/141‑1 (MQ=255)

                                                     aaTGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTCGAAGAAGGCGTTCGAAAAGGTGCCTATACa                                                                                    <  1:305879/141‑1 (MQ=255)

                                                       tGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGt                                                                                  <  2:497754/141‑1 (MQ=255)

                                                         aaaCAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGttt                                                                                >  2:517569/1‑141 (MQ=255)

                                                           aCAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTAt                                                                              <  1:620342/141‑1 (MQ=255)

                                                            cAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATg                                                                             <  1:566119/141‑1 (MQ=255)

                                                             aaGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATgg                                                                            <  2:332176/141‑1 (MQ=255)

                                                               gagTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGct                                                                          >  2:794460/1‑141 (MQ=255)

                                                                  tAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTg                                                                       <  2:847009/141‑1 (MQ=255)

                                                                   aGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGa                                                                      >  2:1082001/1‑141 (MQ=255)

                                                                   aGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGa                                                                      >  1:869664/1‑141 (MQ=255)

                                                                    gCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGaa                                                                     <  1:386509/141‑1 (MQ=255)

                                                                    gCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGaa                                                                     >  1:151987/1‑141 (MQ=255)

                                                                      aTGGGAAGTTGCCTTAGACTCTGTATCTGCACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAaga                                                                   <  2:757956/141‑1 (MQ=255)

                                                                          gAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGaca                                                               <  1:771353/141‑1 (MQ=255)

                                                                            aGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACAcc                                                             <  2:891619/141‑1 (MQ=255)

                                                                             gTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGCGCCTATACAGTTTATGGCTCTGAAGAGACACCa                                                            >  2:719182/1‑141 (MQ=255)

                                                                                  cTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAAtt                                                       <  1:123695/141‑1 (MQ=255)

                                                                                  cTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAAtt                                                       <  1:170167/141‑1 (MQ=255)

                                                                                   ttAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCAGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTc                                                      <  1:543767/141‑1 (MQ=255)

                                                                                    tAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTcc                                                     >  1:568002/1‑141 (MQ=255)

                                                                                       aatctgctgctACTCCTGCTTGATTAGTATTGACACGTCAAAACTTTCCGGTATTAGCTG‑TACCAGAAGATTTAGTTGAAGAAGGCGTTCGAAAAGGTGCTTATACAGTGTATGGCTCTGAAGAGACACCAGATTTCCtaa                                                  <  2:755590/132‑2 (MQ=255)

                                                                                           tgaatctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTattatt                                              >  1:34646/1‑141 (MQ=255)

                                                                                              atctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTTTTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGc                                           >  2:135819/1‑141 (MQ=255)

                                                                                              atctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGc                                           >  1:687491/1‑141 (MQ=255)

                                                                                              atctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGc                                           <  2:415659/141‑1 (MQ=255)

                                                                                              atctACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGAGG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGc                                           <  1:822624/141‑1 (MQ=255)

                                                                                                  acacCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCa                                       >  1:21362/1‑141 (MQ=255)

                                                                                                     ccTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGt                                    <  1:703636/141‑1 (MQ=255)

                                                                                                      cTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGtt                                   >  1:1035428/1‑141 (MQ=255)

                                                                                                          ttCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGATGAAGAAGGGTTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGa                               <  1:102127/141‑1 (MQ=255)

                                                                                                            cATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAg                             <  1:863665/141‑1 (MQ=255)

                                                                                                             aTTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGt                            >  1:167955/1‑141 (MQ=255)

                                                                                                              ttAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGtt                           <  2:1053160/141‑1 (MQ=255)

                                                                                                               tAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTa                          >  2:146819/1‑141 (MQ=255)

                                                                                                                aGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCATTAGGGTTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAg                         >  1:247949/1‑141 (MQ=255)

                                                                                                                  tATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTc                       <  2:382859/141‑1 (MQ=255)

                                                                                                                   aTTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCt                      >  1:649378/1‑141 (MQ=255)

                                                                                                                      gACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGc                   >  1:370407/1‑141 (MQ=255)

                                                                                                                        cacGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAg                 <  2:717816/141‑1 (MQ=255)

                                                                                                                        cacGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAg                 <  1:952918/141‑1 (MQ=255)

                                                                                                                         acGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGt                <  1:593083/141‑1 (MQ=255)

                                                                                                                             cAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGaa            <  1:1081062/141‑1 (MQ=255)

                                                                                                                              aaaaCTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAg           >  2:732711/1‑141 (MQ=255)

                                                                                                                              aaaaCTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAg           <  2:956504/141‑1 (MQ=255)

                                                                                                                                  cTTACCGGTTTTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATGCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAgctgc       <  2:984074/141‑1 (MQ=255)

                                                                                                                                    tACCTGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTa     <  1:38601/141‑1 (MQ=255)

                                                                                                                                    tACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTACTATTATTAGCTTCAGGTTCAGAAGTTAGTCGTGCAGTTGAAGCTGCTa     <  2:1010499/141‑1 (MQ=255)

                                                                                                                                     aCCGGTTTTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaa    <  2:595128/141‑1 (MQ=255)

                                                                                                                                     aCCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaa    <  2:1048362/141‑1 (MQ=255)

                                                                                                                                      ccGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaaa   <  1:740018/141‑1 (MQ=255)

                                                                                                                                      ccGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTaaa   >  2:971858/1‑141 (MQ=255)

                                                                                                                                       cGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAg  >  1:191836/1‑141 (MQ=255)

                                                                                                                                       cGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAg  <  1:34364/141‑1 (MQ=255)

TAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAAACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTGACACGTCAAAACTTACCGGTATTAGATG‑TACCAGAAGATGTAGTTGAAGAAGGCGTTCGAAAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCAGGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAG  >  CP000730/1371903‑1372178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`