BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,458,321	T→C	5.2%	T14A (ACA→GCA)	USA300HOU_1354 ←	phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,458,321	0	T	C	5.2%	281.4 / 3.3	95	T14A (ACA→GCA)	USA300HOU_1354	phosphatase
Reads supporting (aligned to +/- strand): ref base T (52/38); new base C (3/2); total (55/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TTCACCTACTTTAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATATTATTGATA  >  CP000730/1458181‑1458458

ttCACCTACTTTAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGt                                                                                                                                           <  1:104695/141‑1 (MQ=255)

   aCCTACTTTAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCCCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGG‑AAAACGCGATAATTCCAAAAACCAATGTaaaa                                                                                                                                       >  2:694885/1‑141 (MQ=255)

    ccTACTTTAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTaaaa                                                                                                                                       <  2:361754/141‑1 (MQ=255)

    ccTACTTTAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTaaaa                                                                                                                                       <  1:192184/141‑1 (MQ=255)

         tttAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGAt                                                                                                                                  <  1:113306/141‑1 (MQ=255)

          ttAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATg                                                                                                                                 >  2:151730/1‑141 (MQ=255)

             gtagCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGc                                                                                                                              >  1:23947/1‑141 (MQ=255)

              tagCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCa                                                                                                                             <  2:239760/141‑1 (MQ=255)

              tagCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCa                                                                                                                             >  2:340679/1‑141 (MQ=255)

              tagCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCa                                                                                                                             <  1:225984/141‑1 (MQ=255)

               agCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCaa                                                                                                                            >  2:116528/1‑141 (MQ=255)

                       gCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATAc                                                                                                                    >  2:1032822/1‑141 (MQ=255)

                       gCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATAc                                                                                                                    <  2:848904/141‑1 (MQ=255)

                       gCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGAAAAGAGATAc                                                                                                                    >  1:897574/1‑141 (MQ=255)

                         aTGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGt                                                                                                                  <  2:88215/141‑1 (MQ=255)

                         aTGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGt                                                                                                                  <  1:599279/141‑1 (MQ=255)

                         aTGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGt                                                                                                                  <  2:910566/141‑1 (MQ=255)

                           gATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTCTCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGttt                                                                                                                >  1:294211/1‑141 (MQ=255)

                           gATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGttt                                                                                                                <  2:672291/141‑1 (MQ=255)

                            aTAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTc                                                                                                               >  2:480560/1‑141 (MQ=255)

                            aTAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTc                                                                                                               <  1:713329/141‑1 (MQ=255)

                              aGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCa                                                                                                             <  1:354625/141‑1 (MQ=255)

                                    cGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACt                                                                                                       >  1:275243/1‑141 (MQ=255)

                                         taaAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATaa                                                                                                  <  2:965577/141‑1 (MQ=255)

                                         taaAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATaa                                                                                                  >  2:874601/1‑141 (MQ=255)

                                          aaaGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATaaa                                                                                                 >  2:876011/1‑141 (MQ=255)

                                          aaaGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTAATTAGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATaaa                                                                                                 <  2:533843/141‑1 (MQ=255)

                                           aaGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAg                                                                                                <  1:787896/141‑1 (MQ=255)

                                            aGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGc                                                                                               <  2:846662/141‑1 (MQ=255)

                                            aGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGc                                                                                               <  2:964787/141‑1 (MQ=255)

                                               tctctGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCAtt                                                                                            >  1:452375/1‑141 (MQ=255)

                                                   tgatgaATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTAccc                                                                                        >  1:268132/1‑141 (MQ=255)

                                                    gatgaATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTAcccc                                                                                       <  1:388735/141‑1 (MQ=255)

                                                    gatgaATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTAcccc                                                                                       <  1:388607/141‑1 (MQ=255)

                                                          tatataAATTCATAAACTTCATTATCAATCCATTTCCCTAGTCTTGATTCGTGGAAACACGCGATAATTCCAAAAACCAATGCAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTa                                                                                 >  2:646272/1‑141 (MQ=255)

                                                          tataCAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTa                                                                                 >  1:451055/1‑141 (MQ=255)

                                                            taCAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAAt                                                                               >  2:334768/1‑141 (MQ=255)

                                                            taCAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAAt                                                                               >  2:780241/1‑141 (MQ=255)

                                                               aaaCTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCa                                                                            <  1:181657/141‑1 (MQ=255)

                                                               aaaCTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCa                                                                            <  2:260425/141‑1 (MQ=255)

                                                                    cATAAACTTCATTATCAATCCATTTCCCCCATCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGCGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCg                                                                       <  1:237172/141‑1 (MQ=255)

                                                                    cATAAACTTCATTATCAATCCATTTCCCAAGTCTTGGTTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCg                                                                       >  1:886541/1‑141 (MQ=255)

                                                                    cATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCg                                                                       <  1:67260/141‑1 (MQ=255)

                                                                     aTAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGt                                                                      >  1:612253/1‑141 (MQ=255)

                                                                        aaCTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCtt                                                                   >  1:355779/1‑141 (MQ=255)

                                                                        aaCTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCtt                                                                   >  2:314469/1‑141 (MQ=255)

                                                                        aaCTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCtt                                                                   >  2:55412/1‑141 (MQ=255)

                                                                         aCTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCttt                                                                  >  1:4916/1‑141 (MQ=255)

                                                                           ttCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTaa                                                                <  2:995413/141‑1 (MQ=255)

                                                                            tCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAat                                                               <  1:462351/141‑1 (MQ=255)

                                                                            tCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAat                                                               <  2:418941/141‑1 (MQ=255)

                                                                              aTTATCAATCCATTTCCCTAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGCAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAatca                                                             >  1:158981/1‑141 (MQ=255)

                                                                              aTTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAatca                                                             >  1:251252/1‑141 (MQ=255)

                                                                              aTTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAatca                                                             >  2:608076/1‑141 (MQ=255)

                                                                                tataactCCATTTCCCAAGTCTTGATTCGTGGAAAACCGCGATAATTGCAAAAACCAATGTAAAAACGATGCGCAGAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATCCAATGCGTTTTTAatcatc                                                           >  2:123332/7‑141 (MQ=255)

                                                                                 aTCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCt                                                          <  1:276231/141‑1 (MQ=255)

                                                                                  tCAATCCATTTCCCTAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGCAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCtt                                                         <  2:388806/141‑1 (MQ=255)

                                                                                  tCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCtt                                                         <  1:314469/141‑1 (MQ=255)

                                                                                  tCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCtt                                                         <  2:275678/141‑1 (MQ=255)

                                                                                  tCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCtt                                                         <  2:275500/141‑1 (MQ=255)

                                                                                    aaTCCATTTCCCAAGTCTTGATTCGTGGAACAACGCGATAATTCCAAACACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTAt                                                       >  2:115506/1‑141 (MQ=255)

                                                                                    aaTCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTAt                                                       >  1:448613/1‑141 (MQ=255)

                                                                                    aaTCAATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTAt                                                       >  2:313374/1‑141 (MQ=255)

                                                                                      tCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCa                                                     >  2:183541/1‑141 (MQ=255)

                                                                                      tCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCa                                                     >  2:133584/1‑141 (MQ=255)

                                                                                       ccATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCaa                                                    <  1:521663/141‑1 (MQ=255)

                                                                                        cATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCaaa                                                   <  1:712464/141‑1 (MQ=255)

                                                                                           ttCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATaa                                                >  1:663763/1‑141 (MQ=255)

                                                                                            tCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATaaa                                               >  1:1072326/1‑141 (MQ=255)

                                                                                             cccAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAg                                              >  1:662005/1‑141 (MQ=255)

                                                                                              ccAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGc                                             >  1:832901/1‑141 (MQ=255)

                                                                                               cAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGct                                            <  2:415901/141‑1 (MQ=255)

                                                                                                 aGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGcttc                                          >  2:182259/1‑141 (MQ=255)

                                                                                                   tCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGcttctt                                        >  1:351592/1‑141 (MQ=255)

                                                                                                    cTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCttt                                       <  2:101373/141‑1 (MQ=255)

                                                                                                       gATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGt                                    >  1:1065525/1‑141 (MQ=255)

                                                                                                       gATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGt                                    >  1:139135/1‑141 (MQ=255)

                                                                                                        aTTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGta                                   <  2:683458/141‑1 (MQ=255)

                                                                                                         ttCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGtat                                  >  2:667661/1‑141 (MQ=255)

                                                                                                          gctggatcaaaCGCGATAATTCCAAAAACCAATGCAAAAACGATGAGCAAAGAGATACGAACCCATTGACTCACAAAGCATTACCCCCCATTAATTCAATGAGTCTTTAATCATCTTATCACATAAAGCTACTTTCGtata                                 >  2:460934/9‑141 (MQ=255)

                                                                                                              ggAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACtt                             >  1:924326/1‑141 (MQ=255)

                                                                                                                aaaaaCGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTg                           >  2:899503/1‑141 (MQ=255)

                                                                                                                 aaacCGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGt                          >  2:539644/1‑141 (MQ=255)

                                                                                                                 aaaaCGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGt                          >  2:521856/1‑141 (MQ=255)

                                                                                                                   aaCGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTc                        >  2:100399/1‑141 (MQ=255)

                                                                                                                      gcgATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATa                     >  1:1063069/1‑141 (MQ=255)

                                                                                                                         aTAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGa                  >  1:616352/1‑141 (MQ=255)

                                                                                                                          tAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGAt                 <  2:145168/141‑1 (MQ=255)

                                                                                                                           aattcgcaagccctaTGTAAAAACGATGAGCAAAGCGATACGTTTCCCTTGACTCATAAAGCATTACCCCCCATTAATTCACTGCGTCTCTAATCTTCTCATCAAATAAAGCTTCTTTCTTATAACTTTGTTCATACGAtt                <  2:193555/127‑1 (MQ=255)

                                                                                                                           aaTTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGAtt                <  2:990341/141‑1 (MQ=255)

                                                                                                                           aaTTCCAAAAACCAATGCAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTA‑CCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGAttt               <  1:218535/141‑1 (MQ=255)

                                                                                                                                      ccAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATattattt     >  2:215833/1‑140 (MQ=255)

                                                                                                                                       cAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATATTATTGa    >  1:201482/1‑141 (MQ=255)

                                                                                                                                       cAAAGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATAATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATATTATTGa    >  1:530195/1‑141 (MQ=255)

                                                                                                                                         aTGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATATTATTGATa  >  1:800364/1‑141 (MQ=255)

                                                                                                                                         aTGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATATTATTGATa  >  2:228149/1‑141 (MQ=255)

TTCACCTACTTTAGTAGCCCCAAGCATGATAGATGTCGTAATAAAGCTCTCTGATGAATATACAAACTCATAAACTTCATTATCAATCCATTTCCCAAGTCTTGATTCGTGGAAAAACGCGATAATTCCAAAAACCAATGTAAAAACGATGAGCAAAGAGATACGTTTCCATTGACTCATAAAGCATTACCCCCCATTAATTCAATGCGTCTTTAATCATCTTATCAAATAAAGCTTCTTTCGTATAACTTTGTTCATACGATTTCATATTATTGATA  >  CP000730/1458181‑1458458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`