Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,509,367	C→T	7.1%	E1008K (GAG→AAG)	ypbR ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,509,367	0	C	T	7.1%	236.6 / 5.1	80	E1008K (GAG→AAG)	ypbR	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/40);  new base T (2/4);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

TGTTTAGGCAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTCATTT  >  CP000730/1509228‑1509507                                                                                                                                            |                                                                                                                                              tGTTTAGGCAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCa                                                                                                                                              >  2:501932/1‑141 (MQ=255)     tAGGCAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGa                                                                                                                                          <  1:111697/141‑1 (MQ=255)       ggCAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGt                                                                                                                                        >  1:1040786/1‑141 (MQ=255)         cAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTg                                                                                                                                      >  2:500725/1‑141 (MQ=255)         cAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTg                                                                                                                                      >  1:898620/1‑141 (MQ=255)               aTTTAGCATATCATTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAACAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTTAGTGAGTTGCttatt                                                                                                                                <  1:696959/141‑1 (MQ=255)               aTTTAGCATATCATTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAACAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTTAGTGAGTTGCttatt                                                                                                                                <  1:696817/141‑1 (MQ=255)                 ttAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTaa                                                                                                                              >  2:44338/1‑141 (MQ=255)                    gCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGt                                                                                                                           <  1:1067475/141‑1 (MQ=255)                    gCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGACTAGTGAGTTGCTTATTAAAGt                                                                                                                           >  1:1080843/1‑141 (MQ=255)                     cATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTa                                                                                                                          <  2:274620/141‑1 (MQ=255)                       tatCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑tt                                                                                                                       <  1:234161/141‑1 (MQ=255)                       tatCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑tt                                                                                                                       <  2:987163/141‑1 (MQ=255)                            ttGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑ttttttt                                                                                                                  <  2:927754/141‑1 (MQ=255)                               aaaTCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTtata                                                                                                               >  1:962602/1‑141 (MQ=255)                                 aTCGATGTGCAATAATGGTTGCTCTATATTTGCTGAGTGAAAGTGAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTTCTTATTAAAGTA‑TTTTTTtatatg                                                                                                             >  2:381459/1‑139 (MQ=255)                                      tgtgCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTg                                                                                                        >  1:145278/1‑141 (MQ=255)                                       gtgCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGt                                                                                                       <  1:433216/141‑1 (MQ=255)                                            ataatGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTa                                                                                                  <  1:554759/141‑1 (MQ=255)                                             taatGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTaa                                                                                                 <  2:713108/141‑1 (MQ=255)                                              aatGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACTTTCTGTAATTaaa                                                                                                <  2:874534/141‑1 (MQ=255)                                                 ggTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGAt                                                                                             <  2:15696/141‑1 (MQ=255)                                                    tGCTCTATATAAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGc                                                                                          <  2:913758/141‑1 (MQ=255)                                                     gCTCTATCTTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTCATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGct                                                                                         >  2:964964/1‑141 (MQ=255)                                                      ctctATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGctc                                                                                        >  1:711609/1‑141 (MQ=255)                                                      ctctATATTAGCTGATTCAAAGGTAAACTGAGGATTAATAAGGACATGTAAATCTGCTAAGTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑GTTTTTTATACGCGCTGTAATTAAAGATTGctc                                                                                        >  2:918730/1‑141 (MQ=255)                                                       tctATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGctct                                                                                       >  2:925544/1‑141 (MQ=255)                                                        ctATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTTTGTAATTAAAGATGGCTCTa                                                                                      >  1:859692/1‑141 (MQ=255)                                                        ctATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTa                                                                                      <  2:483175/141‑1 (MQ=255)                                                           tatTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTaaaa                                                                                   <  1:727666/141‑1 (MQ=255)                                                            atTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTaaaaa                                                                                  <  1:440073/141‑1 (MQ=255)                                                               aGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACaa                                                                               >  2:996882/1‑141 (MQ=255)                                                                  tGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCg                                                                            >  1:668006/1‑141 (MQ=255)                                                                  tGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGAATGCACTAAAAACAATCg                                                                            <  2:72058/141‑1 (MQ=255)                                                                      tCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTTTAAACACTATCGTtgt                                                                        >  2:914746/1‑140 (MQ=255)                                                                      tCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTtga                                                                        <  2:1002178/141‑1 (MQ=255)                                                                      tCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTtga                                                                        <  2:420046/141‑1 (MQ=255)                                                                          aGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGaa                                                                    >  1:799988/1‑141 (MQ=255)                                                                           gTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAAt                                                                   <  1:847443/141‑1 (MQ=255)                                                                           gTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAAt                                                                   <  1:496863/141‑1 (MQ=255)                                                                            ttAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAAtt                                                                  >  1:760051/1‑141 (MQ=255)                                                                            ttAAACTGAGGATTAATAATGACATGTAAATCTGCTAATGGTTGAACGAGTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAAGCGTTGATGAAtt                                                                  >  2:918939/1‑141 (MQ=255)                                                                              aaaCTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTg                                                                <  2:364856/141‑1 (MQ=255)                                                                                       aTTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGta                                                       <  2:758426/141‑1 (MQ=255)                                                                                        ttAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGtat                                                      <  1:986562/141‑1 (MQ=255)                                                                                          aataatGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATTGTTGATGAATTTGATCTAAGTATAc                                                    >  2:517460/1‑141 (MQ=255)                                                                                               tGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCg                                               >  1:911855/1‑141 (MQ=255)                                                                                                 aCATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTATTTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGt                                              <  1:868193/141‑1 (MQ=255)                                                                                                  cATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAg                                            <  2:433128/141‑1 (MQ=255)                                                                                                  cATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAg                                            >  2:959154/1‑141 (MQ=255)                                                                                                   aTGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGa                                           <  1:119314/141‑1 (MQ=255)                                                                                                      tAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGacac                                        <  1:489559/141‑1 (MQ=255)                                                                                                      tAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGacac                                        <  1:489961/141‑1 (MQ=255)                                                                                                         aTCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGAAAAAAAAAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACActtt                                     <  2:430380/141‑1 (MQ=255)                                                                                                          tCTGCTAATTGTTGAACGATTGGTGCAATTTTCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACActttc                                    <  2:806826/141‑1 (MQ=255)                                                                                                          tCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACActttc                                    <  2:163380/141‑1 (MQ=255)                                                                                                              cTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACtttctttt                                >  1:75865/1‑141 (MQ=255)                                                                                                              cTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACtttctttt                                >  1:725502/1‑141 (MQ=255)                                                                                                               tAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACttgcttttc                               <  2:1079656/141‑1 (MQ=255)                                                                                                                aaTTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACtttcttttct                              <  1:1027443/141‑1 (MQ=255)                                                                                                                 aTTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTTCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTttcttttctt                             >  1:227061/1‑141 (MQ=255)                                                                                                                 aTTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTttcttttctt                             <  2:1045743/141‑1 (MQ=255)                                                                                                                  ttgttgAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTttcttc                            <  2:817648/141‑1 (MQ=255)                                                                                                                   tgttgAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCa                           >  1:952385/1‑141 (MQ=255)                                                                                                                   tgttgAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCa                           <  2:91708/141‑1 (MQ=255)                                                                                                                     ttgAACGATTGGTGCAATTTGCTTAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTTTCGTAGACACTTTCTTTTCTTCAtt                         <  1:325820/141‑1 (MQ=255)                                                                                                                         aCGATTGGTGCAATTTGCTTAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTTTCGTAGACACTTTCTTTTCTTCATTaaaa                     >  1:756616/1‑141 (MQ=255)                                                                                                                         aCGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTaaaa                     <  1:787663/141‑1 (MQ=255)                                                                                                                               ggTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTa               >  2:175154/1‑141 (MQ=255)                                                                                                                                 tGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTAtt             >  1:600982/1‑141 (MQ=255)                                                                                                                                  gCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTAttt            >  1:447102/1‑141 (MQ=255)                                                                                                                                     aTTTTCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGc         <  2:567540/141‑1 (MQ=255)                                                                                                                                     aTTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGc         >  1:23979/1‑141 (MQ=255)                                                                                                                                     aTTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGc         <  1:384941/141‑1 (MQ=255)                                                                                                                                      tttGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCg        >  1:245286/1‑141 (MQ=255)                                                                                                                                       ttGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGt       <  2:924377/141‑1 (MQ=255)                                                                                                                                        tGCTTAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTTTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTc      <  1:239669/141‑1 (MQ=255)                                                                                                                                         gCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTCa     >  2:261840/1‑141 (MQ=255)                                                                                                                                         gCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTCa     >  2:299050/1‑141 (MQ=255)                                                                                                                                           tCAGTGAGATGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGCCACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTCAtt   >  2:504485/1‑141 (MQ=255)                                                                                                                                            cAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTCAttt  >  1:40428/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                              TGTTTAGGCAATGCATTTAGCATATCGTTGAAATCGATGTGCAATAATGGTTGCTCTATATTAGCTGATTCAAAGTTAAACTGAGGATTAATAATGACATGTAAATCTGCTAATTGTTGAACGATTGGTGCAATTTGCTCAGTGAGTTGCTTATTAAAGTA‑TTTTTTTATACGTTCTGTAATTAAAGATTGCTCTAAAAACAATCGTTGATGAATTTGATCTAAGTATACTCTCGTAGACACTTTCTTTTCTTCATTAAAATCACTATTTTGCGTCATTT  >  CP000730/1509228‑1509507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑