Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,517,372 C→A 7.8% D115Y (GAT→TAT)  USA300HOU_1385 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,517,3720CA 7.8% 319.5 / 8.5 98D115Y (GAT→TAT) USA300HOU_1385hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/50);  new base A (2/6);  total (42/56)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTA  >  CP000730/1517232‑1517509
                                                                                                                                            |                                                                                                                                         
tcccATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTAGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATc                                                                                                                                           <  2:517675/140‑1 (MQ=255)
acCCATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATc                                                                                                                                           <  2:819001/141‑1 (MQ=255)
   cATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGt                                                                                                                                        <  1:985945/141‑1 (MQ=255)
    aTTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTa                                                                                                                                       >  1:1013531/1‑141 (MQ=255)
      tCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAcc                                                                                                                                     <  1:751211/141‑1 (MQ=255)
      tCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAcc                                                                                                                                     <  1:996839/141‑1 (MQ=255)
       cTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTAccc                                                                                                                                    >  2:1042631/1‑141 (MQ=255)
         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  >  2:515201/1‑141 (MQ=255)
         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  <  2:462793/141‑1 (MQ=255)
         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  <  2:993249/141‑1 (MQ=255)
         aaTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctc                                                                                                                                  <  2:158524/141‑1 (MQ=255)
          aTCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCctct                                                                                                                                 >  2:494186/1‑141 (MQ=255)
           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTTCTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCtt                                                                                                                                >  2:534746/1‑141 (MQ=255)
           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTTACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCtt                                                                                                                                >  1:803765/1‑141 (MQ=255)
           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCtt                                                                                                                                >  1:925349/1‑141 (MQ=255)
           tCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCtt                                                                                                                                <  2:655347/141‑1 (MQ=255)
           tCCTTCATCCAACAGTCGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTAcc                                                                                                                                     <  1:325526/136‑1 (MQ=255)
           tCCTTCATCCAACAGTCGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTAcc                                                                                                                                     >  2:325526/1‑136 (MQ=255)
                cATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGtt                                                                                                                           <  1:673244/141‑1 (MQ=255)
                cATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGtt                                                                                                                           >  1:594686/1‑141 (MQ=255)
                  tCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTAc                                                                                                                         <  1:101728/141‑1 (MQ=255)
                  tCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCAAAATCTATAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTAc                                                                                                                         <  2:363111/141‑1 (MQ=255)
                    cAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTACGc                                                                                                                       <  2:470699/141‑1 (MQ=255)
                         gttgttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCt                                                                                                                  <  1:465733/141‑1 (MQ=255)
                          ttgttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTa                                                                                                                 <  1:632234/141‑1 (MQ=255)
                          ttgttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTa                                                                                                                 <  2:280199/141‑1 (MQ=255)
                            gttCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAat                                                                                                               <  1:508768/141‑1 (MQ=255)
                              tCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAatat                                                                                                             <  1:35940/141‑1 (MQ=255)
                                 aTTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATAtt                                                                                                          <  1:474480/141‑1 (MQ=255)
                                  ttttATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATAttt                                                                                                         <  1:434393/141‑1 (MQ=255)
                                     tATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGt                                                                                                      <  2:423296/141‑1 (MQ=255)
                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     >  1:753327/1‑141 (MQ=255)
                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     >  1:705033/1‑141 (MQ=255)
                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     >  2:676035/1‑141 (MQ=255)
                                      aTGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTa                                                                                                     <  2:500388/141‑1 (MQ=255)
                                              taAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCatat                                                                                             <  1:236362/141‑1 (MQ=255)
                                                aaTTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAAACATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTg                                                                                           <  1:226507/141‑1 (MQ=255)
                                                 aTTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGa                                                                                          >  2:793213/1‑141 (MQ=255)
                                                  ttGTTTTGAAGAATGTACTTCAGGTGCGTCATCTGTAGAAAGTTTTAACGTGGATGATTTGTATCCTGTGACAGTAACTTTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGaa                                                                                         <  1:1081268/141‑1 (MQ=255)
                                                  ttGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGaa                                                                                         >  1:598196/1‑141 (MQ=255)
                                                   tGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAac                                                                                        <  2:414541/141‑1 (MQ=255)
                                                    gTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAaca                                                                                       <  2:1041799/141‑1 (MQ=255)
                                                    gTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAaca                                                                                       >  2:683209/1‑141 (MQ=255)
                                                      tttAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACt                                                                                     <  2:616081/141‑1 (MQ=255)
                                                       ttAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACtt                                                                                    >  2:471402/1‑141 (MQ=255)
                                                        tAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACttt                                                                                   <  1:838391/141‑1 (MQ=255)
                                                         aaaTAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGTCATCTAATATATTTTGTAAATCATATTGAACACtttt                                                                                  <  2:1042458/141‑1 (MQ=255)
                                                         aaaTAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACtttt                                                                                  <  2:54602/141‑1 (MQ=255)
                                                         aaaTAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACtttt                                                                                  <  1:179707/141‑1 (MQ=255)
                                                           ttaatGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTaa                                                                                <  2:447449/140‑1 (MQ=255)
                                                              atGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCt                                                                             <  2:746673/141‑1 (MQ=255)
                                                              atGTACTCCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTGTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCt                                                                             >  1:45052/1‑141 (MQ=255)
                                                               tGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtt                                                                            >  1:115906/1‑141 (MQ=255)
                                                               tGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtt                                                                            <  1:306787/141‑1 (MQ=255)
                                                               tGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCtt                                                                            >  2:398627/1‑141 (MQ=255)
                                                                gTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCttt                                                                           <  1:90685/141‑1 (MQ=255)
                                                                  aCTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTc                                                                         >  1:211851/1‑141 (MQ=255)
                                                                     tCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGGACACTTTTAAGCTTTTCtat                                                                      >  2:296385/1‑141 (MQ=255)
                                                                       agttGCGTCATCTATAAGAATGTTTAATTCATATGATTTGTAACCTTTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCtatat                                                                    <  1:1033253/138‑2 (MQ=255)
                                                                        ggTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaa                                                                   >  1:391406/1‑141 (MQ=255)
                                                                        ggTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaa                                                                   >  2:462195/1‑141 (MQ=255)
                                                                        ggTGCGGCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaa                                                                   >  2:509252/1‑141 (MQ=255)
                                                                          tGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTataaata                                                                 >  2:155217/1‑141 (MQ=255)
                                                                                    ttAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACt                                                       <  2:1071145/141‑1 (MQ=255)
                                                                                      aaaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTg                                                     >  1:51757/1‑141 (MQ=255)
                                                                                      aaaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTg                                                     <  2:1054923/141‑1 (MQ=255)
                                                                                      aaaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTg                                                     <  2:541294/141‑1 (MQ=255)
                                                                                       aaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGt                                                    >  1:13074/1‑141 (MQ=255)
                                                                                       aaaaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGt                                                    <  2:642303/141‑1 (MQ=255)
                                                                                         aaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTc                                                  <  1:745114/141‑1 (MQ=255)
                                                                                         aaTGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATAAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGGACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTc                                                  <  1:296385/141‑1 (MQ=255)
                                                                                           tGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGc                                                <  1:793213/141‑1 (MQ=255)
                                                                                           tGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGc                                                <  2:288808/141‑1 (MQ=255)
                                                                                            gTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCt                                               >  1:186325/1‑141 (MQ=255)
                                                                                             tttAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCAAAAGCACACTCCATAACTACGTGCAATTCTTTGGTATGTATATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCtt                                              <  1:213827/141‑1 (MQ=255)
                                                                                              ttAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTa                                             >  1:397750/1‑141 (MQ=255)
                                                                                                aaTTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTaaa                                           <  1:184505/141‑1 (MQ=255)
                                                                                                   tCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATg                                        >  2:719619/1‑141 (MQ=255)
                                                                                                      aaTGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACa                                     >  2:330310/1‑141 (MQ=255)
                                                                                                              gTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAg                             >  1:58126/1‑141 (MQ=255)
                                                                                                              gTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAg                             >  1:74821/1‑141 (MQ=255)
                                                                                                                 aCCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGaaa                          >  2:408986/1‑141 (MQ=255)
                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTGCCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  1:772732/141‑1 (MQ=255)
                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  1:709818/141‑1 (MQ=255)
                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  1:64638/141‑1 (MQ=255)
                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  2:677714/141‑1 (MQ=255)
                                                                                                                  ccTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAg                         <  2:985470/141‑1 (MQ=255)
                                                                                                                    tGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgtt                       >  1:434016/1‑141 (MQ=255)
                                                                                                                     gTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgttg                      <  2:424055/141‑1 (MQ=255)
                                                                                                                      ttACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgttgt                     <  2:4675/141‑1 (MQ=255)
                                                                                                                       tACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAgttgtt                    >  2:998945/1‑141 (MQ=255)
                                                                                                                                 gTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGc          <  1:190396/141‑1 (MQ=255)
                                                                                                                                 gTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGc          <  1:398627/141‑1 (MQ=255)
                                                                                                                                   tttAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCaa        >  2:209717/1‑141 (MQ=255)
                                                                                                                                     tAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATc      >  1:260520/1‑141 (MQ=255)
                                                                                                                                     tAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATc      >  2:1032684/1‑141 (MQ=255)
                                                                                                                                      aaCCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCa     >  1:15685/1‑141 (MQ=255)
                                                                                                                                       aCCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAg    >  1:1070474/1‑141 (MQ=255)
                                                                                                                                        ccATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGt   <  1:937861/141‑1 (MQ=255)
                                                                                                                                         cATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTa  >  1:385942/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
ACCCATTCTAATCCTTCATCCAACAGTTGTTCAATTTTATGTTTTATAAATTGTTTTAAATAATGTACTTCAGGTGCGTCATCTTTAAAAATGTTTAATTCGAATGATTTGTAACCTGTTACATAAACTGTTTTAACCATCAGTACCCTCTTTTGTTACGCCATCTAATATATTTTGTAAATCATATTGAACACTTTTAAGCTTTTCTATAAATAACTTTCGACTTGTTCGCTTAAAATGACATTCGACAGAAAGTTGTTCAATGTTAGCAATCAGTA  >  CP000730/1517232‑1517509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑