Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,538,398	T→G	6.1%	G168G (GGA→GGC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,538,398	0	T	G	6.1%	238.9 / 7.3	82	G168G (GGA→GGC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (38/39);  new base G (3/2);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

TTGAATCTCCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACGATGATT  >  CP000730/1538262‑1538538                                                                                                                                         |                                                                                                                                             ttGAATCTCCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCAc                                                                                                                                          <  1:631281/141‑1 (MQ=255)  tGAATCTCCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGCTTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCAcc                                                                                                                                         >  2:249711/1‑141 (MQ=255)     aTCTCCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTACTTCAACCTAt                                                                                                                                      >  2:505510/1‑141 (MQ=255)     aTCTCCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTAt                                                                                                                                      <  2:884042/141‑1 (MQ=255)      tctcCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTAtt                                                                                                                                     <  1:706336/141‑1 (MQ=255)       ctcCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTAttt                                                                                                                                    <  1:38019/141‑1 (MQ=255)           ttCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTcaac                                                                                                                                <  2:711638/141‑1 (MQ=255)               tttttAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAacaaca                                                                                                                            >  1:754740/1‑141 (MQ=255)               tttttAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATGCCACCTATTTCAacaaca                                                                                                                            <  2:957552/141‑1 (MQ=255)                ttttAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAacac                                                                                                                           <  1:676263/141‑1 (MQ=255)                ttttAGCTTAGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAacac                                                                                                                           <  1:676350/141‑1 (MQ=255)                    agtttCGTCAATTTTATTTAGCATTGCTTGTGAGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTTTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACt                                                                                                                       >  2:860198/4‑141 (MQ=255)                    aGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACt                                                                                                                       >  1:290630/1‑141 (MQ=255)                    aGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACt                                                                                                                       >  1:878431/1‑141 (MQ=255)                    aGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACt                                                                                                                       >  1:290590/1‑141 (MQ=255)                     gCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTg                                                                                                                      <  1:874134/141‑1 (MQ=255)                     gCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTg                                                                                                                      >  1:216751/1‑141 (MQ=255)                        tCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGtat                                                                                                                   >  1:74569/1‑141 (MQ=255)                           tCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGtatata                                                                                                                >  2:1057993/1‑141 (MQ=255)                              aTTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCg                                                                                                             >  1:492773/1‑141 (MQ=255)                                 ttATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGata                                                                                                          >  1:162289/1‑141 (MQ=255)                                  tATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGatat                                                                                                         <  1:597867/141‑1 (MQ=255)                                   aTCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATc                                                                                                        >  2:1000720/1‑141 (MQ=255)                                   aTCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTAAAAAATAATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATc                                                                                                        <  2:1071221/141‑1 (MQ=255)                                    tctcGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCt                                                                                                       >  2:313064/1‑141 (MQ=255)                                     ctcGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTa                                                                                                      <  1:159138/141‑1 (MQ=255)                                     ctcGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTa                                                                                                      <  2:627113/141‑1 (MQ=255)                                      tcGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGCATCATAAAAATATTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTaa                                                                                                     >  1:810916/1‑141 (MQ=255)                                        gCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACt                                                                                                   >  1:455389/1‑141 (MQ=255)                                        gCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACt                                                                                                   >  2:61927/1‑141 (MQ=255)                                           ttGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACtgtt                                                                                                <  2:200518/141‑1 (MQ=255)                                                tgtgCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGtaat                                                                                           >  2:65812/1‑141 (MQ=255)                                                  tgCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTCTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGaaataa                                                                                         >  2:475483/1‑141 (MQ=255)                                                     gATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAacac                                                                                      <  2:454418/141‑1 (MQ=255)                                                       tACTGTCATCAATTACACGAACGTCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTTATTCCGCCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACtt                                                                                    <  2:99118/141‑1 (MQ=255)                                                             catcaATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATa                                                                              <  2:400235/141‑1 (MQ=255)                                                                caATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAgg                                                                           <  1:998446/141‑1 (MQ=255)                                                                 aaTTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGc                                                                          <  1:123927/141‑1 (MQ=255)                                                                   ttACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCAc                                                                        >  1:937356/1‑141 (MQ=255)                                                                     acacGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCg                                                                      >  2:543687/1‑141 (MQ=255)                                                                     acacGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCg                                                                      >  2:977252/1‑141 (MQ=255)                                                                       acGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAGCAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGAc                                                                    <  2:66710/141‑1 (MQ=255)                                                                       acGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGAc                                                                    >  1:411249/1‑141 (MQ=255)                                                                          aaCATNATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATCTAGATAGCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGc                                                                 >  1:826748/1‑141 (MQ=255)                                                                             atcatTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAAc                                                              <  1:1041376/141‑1 (MQ=255)                                                                             atcatTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATGCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAAc                                                              <  2:850652/141‑1 (MQ=255)                                                                              tcatTACGATCAAGATTTGCTTTAAATGTTTCTGCATAATCAAAATCTTTCTCTTTCCTTCCCCCCATTTCAAACCGTAGACTGTATATATCGCTATCTAACTGTTGTCATAACACTTTACATACGGCACCGACTGCAACt                                                             <  1:813119/141‑1 (MQ=255)                                                                               catTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTc                                                            <  2:327747/141‑1 (MQ=255)                                                                                atTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCg                                                           <  2:411249/141‑1 (MQ=255)                                                                                atTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCg                                                           <  1:471278/141‑1 (MQ=255)                                                                                atTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGAAACTCg                                                           >  2:796120/1‑141 (MQ=255)                                                                                atTACGATCAAGATTTGCTTTAAATGTTTCTGAACCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCg                                                           <  1:618966/141‑1 (MQ=255)                                                                                  tACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAg                                                         >  2:707373/1‑141 (MQ=255)                                                                                     gATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAgctg                                                      <  1:816461/141‑1 (MQ=255)                                                                                       tCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAgctgct                                                    <  2:526448/141‑1 (MQ=255)                                                                                         aaGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATGCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGt                                                  >  2:103131/1‑141 (MQ=255)                                                                                           gATTTGCTTTAAATGTTTCGGAATCATAAAAATCTTTATCTTTAATTCCACCTATCTCAACAACACGACTGTATATCTCGATCTCTAACTGCTGTAACAACACTTTACACACCGCACCGACTGCAACCCGAGctgctgccc                                                >  2:626439/1‑138 (MQ=255)                                                                                            aTTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTtc                                               <  2:155904/141‑1 (MQ=255)                                                                                                gCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTa                                           >  2:363271/1‑141 (MQ=255)                                                                                                 cTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAg                                          <  1:346500/141‑1 (MQ=255)                                                                                                  tttAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGc                                         >  2:968519/1‑141 (MQ=255)                                                                                                   ttAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCa                                        >  1:439961/1‑141 (MQ=255)                                                                                                   ttAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCa                                        >  1:759561/1‑141 (MQ=255)                                                                                                    tAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATACATCCATATCTAACTGTTGTAATAACACTTTACATAAGGCACCCACTGCAACCCGAGCTGCTGTTTCTCTCGCAg                                       >  2:515196/1‑141 (MQ=255)                                                                                                       aTGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAgatg                                    >  1:605785/1‑141 (MQ=255)                                                                                                           ttCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCg                                <  2:361920/141‑1 (MQ=255)                                                                                                            tCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGc                               >  1:378480/1‑141 (MQ=255)                                                                                                                  tCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGc                         <  2:533848/141‑1 (MQ=255)                                                                                                                  tCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGc                         <  1:966502/141‑1 (MQ=255)                                                                                                                  tCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGc                         <  1:739004/141‑1 (MQ=255)                                                                                                                   cATAAAAATCTTTATCTTTAATTACACCTATTTCAACAACAAGACTGTCTATATCAATATCTAAATGTTGTACTAACACTTACCAAAAGGGAAAGAGTGCAACACGCGCTGCGGTTTCTCTCGCAGATGCTCGCTCTAGcc                        >  1:158090/1‑140 (MQ=255)                                                                                                                    aTAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGcac                       <  2:905053/141‑1 (MQ=255)                                                                                                                     tAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGcaca                      >  1:258901/1‑141 (MQ=255)                                                                                                                       aaaaTCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGTACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACAtt                    >  1:68605/1‑141 (MQ=255)                                                                                                                       aaaaTCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACAtt                    <  2:659270/141‑1 (MQ=255)                                                                                                                        aaaTCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACAttt                   <  1:280175/141‑1 (MQ=255)                                                                                                                        aaaTCTTTATCTTTAATGCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACAttt                   >  2:892678/1‑141 (MQ=255)                                                                                                                           tctttatctttaATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGt                <  1:765012/141‑1 (MQ=255)                                                                                                                            ctttatctttaATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTa               <  1:197786/141‑1 (MQ=255)                                                                                                                              ttatctttaATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTATCTAGCAGATGATCGCTCTAGCACATTTCGTaaa             >  1:1006520/1‑141 (MQ=255)                                                                                                                              ttatctttaATGCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTaaa             >  1:518546/1‑141 (MQ=255)                                                                                                                                   tttaATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACg        <  1:879342/141‑1 (MQ=255)                                                                                                                                      aagtCCCCCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACgatg     <  2:873197/138‑1 (MQ=255)                                                                                                                                        ttCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACgatgat   <  2:290590/141‑1 (MQ=255)                                                                                                                                        ttCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACgatgat   <  2:290630/141‑1 (MQ=255)                                                                                                                                        ttCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACgatgat   <  1:788103/141‑1 (MQ=255)                                                                                                                                         tCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTATCACATTTCGTAAATCACGATGAtt  >  1:1024808/1‑141 (MQ=255)                                                                                                                                         |                                                                                                                                             TTGAATCTCCTTCATTTTTAGCTTCGTCAATTTTATCTCGCATTGCTTGTGCGATACTGTCATCAATTACACGAACATCATTACGATCAAGATTTGCTTTAAATGTTTCTGAATCATAAAAATCTTTATCTTTAATTCCACCTATTTCAACAACACGACTGTATATATCGATATCTAACTGTTGTAATAACACTTTACATAAGGCACCGACTGCAACTCGAGCTGCTGTTTCTCTAGCAGATGATCGCTCTAGCACATTTCGTAAATCACGATGATT  >  CP000730/1538262‑1538538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑