Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,708,413 C→T 5.9% T43I (ACA→ATA)  USA300HOU_1602 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,708,4130CT 5.9% 208.9 / 2.2 67T43I (ACA→ATA) USA300HOU_1602hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base T (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

GAGGATTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATCTTCTTTTTACTAT  >  CP000730/1708274‑1708553
                                                                                                                                           |                                                                                                                                            
gagGATTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACa                                                                                                                                             <  2:723683/141‑1 (MQ=255)
 agGATTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAg                                                                                                                                            <  1:501595/141‑1 (MQ=255)
  ggATTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGc                                                                                                                                           <  1:146658/141‑1 (MQ=255)
    aTTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGcaa                                                                                                                                         <  2:928682/141‑1 (MQ=255)
    aTTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGcaa                                                                                                                                         <  1:207494/141‑1 (MQ=255)
     ttttACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGcaac                                                                                                                                        <  2:346803/141‑1 (MQ=255)
     ttttACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGcaac                                                                                                                                        <  2:346885/141‑1 (MQ=255)
      tttACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTCTAAGCATATGTTTAAAGTTTTCATTTAATATCGAGTTTACAGcaaca                                                                                                                                       <  1:792142/141‑1 (MQ=255)
        tACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAAt                                                                                                                                     >  2:717221/1‑141 (MQ=255)
           aaTGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTAt                                                                                                                                  <  1:474523/141‑1 (MQ=255)
                   aaaaTAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACatat                                                                                                                          <  2:455227/141‑1 (MQ=255)
                         atCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCt                                                                                                                    <  1:580790/141‑1 (MQ=255)
                          tCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTa                                                                                                                   >  1:499831/1‑141 (MQ=255)
                              aaTAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTATAGCAACAATTATGAACATATGTCGCTACAct                                                                                                               <  1:533810/141‑1 (MQ=255)
                               aTAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACActc                                                                                                              <  1:1027468/141‑1 (MQ=255)
                                   tctcGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAAt                                                                                                          >  2:71124/1‑141 (MQ=255)
                                    ctcGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAAtt                                                                                                         >  2:65261/1‑141 (MQ=255)
                                    ctcGCAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCGTTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAAtt                                                                                                         >  2:862478/1‑141 (MQ=255)
                                     tcGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTATAGTTTTCTTTTAATATCGAGTTTATAGCAACAATTATGAACATATGTCGCTACACTCTAAttt                                                                                                        >  2:718825/1‑140 (MQ=255)
                                       gTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTa                                                                                                      >  2:558597/1‑141 (MQ=255)
                                          aCAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGa                                                                                                   <  2:316441/141‑1 (MQ=255)
                                          aCAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGa                                                                                                   >  1:82243/1‑141 (MQ=255)
                                              aGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTta                                                                                               >  2:730556/1‑141 (MQ=255)
                                               gATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTtat                                                                                              <  1:960438/141‑1 (MQ=255)
                                                aTAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTtata                                                                                             >  1:608720/1‑141 (MQ=255)
                                                 tAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTATACAGCAACAATTCTGAACATATGTCGCTACACGCTAATTCTAGGATTTATAc                                                                                            >  2:766493/1‑141 (MQ=255)
                                                        aTACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTc                                                                                     >  2:465999/1‑141 (MQ=255)
                                                            ggCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTa                                                                                 <  2:925186/141‑1 (MQ=255)
                                                             gCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTAc                                                                                >  2:477033/1‑141 (MQ=255)
                                                              cAGTTTTATTGCAATTGCTTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTAcc                                                                               <  1:1026531/141‑1 (MQ=255)
                                                              cAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTAcc                                                                               >  2:698467/1‑141 (MQ=255)
                                                               aGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCt                                                                              >  1:1059528/1‑141 (MQ=255)
                                                                   ttATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGAtg                                                                          >  1:276620/1‑141 (MQ=255)
                                                                     aTTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGAtgtt                                                                        >  1:726217/1‑141 (MQ=255)
                                                                          aaTTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGa                                                                   >  2:163877/1‑141 (MQ=255)
                                                                          aaTTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGa                                                                   <  2:1082012/141‑1 (MQ=255)
                                                                             tGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTATAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGAtgtttttatcaa                                                                <  2:905406/141‑9 (MQ=255)
                                                                              gCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATaaa                                                               >  2:992014/1‑141 (MQ=255)
                                                                              gCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATaaa                                                               >  1:695431/1‑141 (MQ=255)
                                                                              gCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATaaa                                                               <  2:894812/141‑1 (MQ=255)
                                                                                 tcgtTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTATAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAAc                                                            >  2:451190/1‑141 (MQ=255)
                                                                                 tcgtTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAAc                                                            >  1:436792/1‑141 (MQ=255)
                                                                                     ttGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCa                                                        <  2:280284/141‑1 (MQ=255)
                                                                                         cATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGaa                                                    >  1:114738/1‑141 (MQ=255)
                                                                                         cATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGaa                                                    >  2:597814/1‑141 (MQ=255)
                                                                                             ttatttatAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAAtt                                                <  1:992160/141‑1 (MQ=255)
                                                                                                  tataAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTAATAAAAACATCAAGAAAATTATAtt                                           >  1:585939/1‑141 (MQ=255)
                                                                                                   ataAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTc                                          <  2:909124/141‑1 (MQ=255)
                                                                                                    taAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCg                                         >  1:495306/1‑141 (MQ=255)
                                                                                                    taAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCg                                         >  2:443873/1‑141 (MQ=255)
                                                                                                       gTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATg                                      <  2:42922/141‑1 (MQ=255)
                                                                                                       gTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATg                                      >  1:514672/1‑141 (MQ=255)
                                                                                                         atatTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCa                                    >  2:1005627/1‑141 (MQ=255)
                                                                                                         atatTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCa                                    <  2:823637/141‑1 (MQ=255)
                                                                                                            tttttAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCAttt                                 <  2:567832/141‑1 (MQ=255)
                                                                                                              tttAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGCCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATttat                               <  2:794297/141‑1 (MQ=255)
                                                                                                                  aaGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTatc                           >  1:449785/1‑141 (MQ=255)
                                                                                                                      tttCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCg                       <  2:271377/141‑1 (MQ=255)
                                                                                                                        tCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTc                     >  1:839689/1‑141 (MQ=255)
                                                                                                                           tttAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCttt                  <  2:204987/141‑1 (MQ=255)
                                                                                                                                   cGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATcttctt          <  1:383658/141‑1 (MQ=255)
                                                                                                                                   cGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATcttctt          <  1:1040267/141‑1 (MQ=255)
                                                                                                                                   cGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATcttctt          <  1:325740/141‑1 (MQ=255)
                                                                                                                                   cGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATcttctt          >  2:1068533/1‑141 (MQ=255)
                                                                                                                                   cGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATcttctt          <  2:699351/141‑1 (MQ=255)
                                                                                                                                   cGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATcttctt          <  1:950918/141‑1 (MQ=255)
                                                                                                                                        ttACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATCTTCTTTTTAc     <  1:506531/141‑1 (MQ=255)
                                                                                                                                           cAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATCTTCTTTTTACtat  >  2:789372/1‑141 (MQ=255)
                                                                                                                                           cAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATCTTCTTTTTACtat  >  1:877415/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GAGGATTTTACAATGGCTGAAAATAATCAAAATAGTCTCGTAACAAAGATAGCTACATACGGCAGTTTTATTGCAATTGCGTCGTTTGTCATTTTATTTATAAGTATATTTTTAAAGTTTTCATTTAATATCGAGTTTACAGCAACAATTATGAACATATGTCGCTACACTCTAATTCTAGGATTTATACTTATGTCCTTACCTGATGTTGTTGATAAAAACATCAAGAAAATTATATTCGATGCATTTATTATCATCGTCTTTATCTTCTTTTTACTAT  >  CP000730/1708274‑1708553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑