Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,735,527	T→A	5.1%	A236A (GCA→GCT)	hypB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,735,527	0	T	A	5.1%	251.5 / 4.2	79	A236A (GCA→GCT)	hypB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/48);  new base A (2/2);  total (29/50)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

CGAACTAGTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTGAAAATA  >  CP000730/1735388‑1735666                                                                                                                                            |                                                                                                                                              cGAACTAGTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTg                                                                                                                                              >  2:26178/1‑141 (MQ=255)   aaCTAGTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTCCGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGca                                                                                                                                            <  2:149535/141‑1 (MQ=255)   aaCTAGTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGca                                                                                                                                            <  2:160746/141‑1 (MQ=255)       aGTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACaaa                                                                                                                                        <  1:532705/141‑1 (MQ=255)        gTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAAt                                                                                                                                       <  1:596303/141‑1 (MQ=255)        gTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAAt                                                                                                                                       >  2:830498/1‑141 (MQ=255)        gTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAAt                                                                                                                                       <  1:476770/141‑1 (MQ=255)            gttttagttttaTGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAAt                                                                                                                                   >  1:831983/1‑141 (MQ=255)               ttagttttaTGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCca                                                                                                                                <  1:387030/141‑1 (MQ=255)                 agttttaTGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCcaac                                                                                                                              <  1:56312/141‑1 (MQ=255)                 agttttaTGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCcaac                                                                                                                              <  1:440268/141‑1 (MQ=255)                  gttttaTGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCcaaca                                                                                                                             >  2:264277/1‑141 (MQ=255)                       aTGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACt                                                                                                                        <  1:985402/141‑1 (MQ=255)                        tGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACtt                                                                                                                       >  1:492875/1‑141 (MQ=255)                        tGGTGTGGTTACAATTCCCCTACCTCCAACATCAACCCTTTACAAATGAATTATTGCCCTTTGTCTTTAATGCGACGCACTAGAATATAATATAATCCCTAACTCACCACCAAACTTGCCCAAATCAAACCAACCacaccc                                                                                                                       <  2:270136/141‑3 (MQ=255)                         ggTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGACCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTg                                                                                                                      <  1:237908/141‑1 (MQ=255)                          gtgtGGTTACACTTGCCTTACGTGGAAAATTGTCCCGTGCAAAATGATTTATTGCCCGTTGTCGTGAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTgg                                                                                                                     <  1:491029/141‑1 (MQ=255)                            gtgGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGca                                                                                                                   >  2:621931/1‑141 (MQ=255)                                 tACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAg                                                                                                              <  1:171639/141‑1 (MQ=255)                                 tACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAg                                                                                                              >  2:493727/1‑141 (MQ=255)                                     cTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCAt                                                                                                          <  1:582875/141‑1 (MQ=255)                                        gCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTg                                                                                                       <  1:981908/141‑1 (MQ=255)                                               gTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAgg                                                                                                >  1:74769/1‑141 (MQ=255)                                                ttGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGt                                                                                               <  2:724242/141‑1 (MQ=255)                                                ttGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGt                                                                                               <  1:788811/141‑1 (MQ=255)                                                 tGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑g                                                                                            >  1:111938/1‑141 (MQ=255)                                                 tGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑g                                                                                            <  1:748490/141‑1 (MQ=255)                                                  gAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑gg                                                                                           <  2:142675/141‑1 (MQ=255)                                                   aaaaTTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGc                                                                                          >  1:536297/1‑141 (MQ=255)                                                        tATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTg                                                                                     >  1:947737/1‑141 (MQ=255)                                                         aTCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGt                                                                                    <  2:204265/141‑1 (MQ=255)                                                         aTCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGt                                                                                    >  1:1059396/1‑141 (MQ=255)                                                         aTCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGt                                                                                    >  1:550024/1‑141 (MQ=255)                                                          tCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTc                                                                                   <  2:1044002/141‑1 (MQ=255)                                                           cccTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTcc                                                                                  <  1:1024049/141‑1 (MQ=255)                                                           cccTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTcc                                                                                  <  2:214542/141‑1 (MQ=255)                                                           cccTCTCAGAATGAATTAGTGCCCTTTGTCTTTAATGCGACGAACGGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTcc                                                                                  <  1:746211/141‑1 (MQ=255)                                                            ccTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCt                                                                                 <  1:471623/141‑1 (MQ=255)                                                             cTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCtt                                                                                <  2:729489/141‑1 (MQ=255)                                                              tttCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCttt                                                                               >  2:1059049/1‑141 (MQ=255)                                                                    aaTGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGAtt                                                                         >  1:25617/1‑141 (MQ=255)                                                                    aaTGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGAtt                                                                         <  2:851238/141‑1 (MQ=255)                                                                    aaTGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGAtt                                                                         >  1:700899/1‑141 (MQ=255)                                                                       gAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGTCCGGCATT‑‑TCCTTTTCGATTGAt                                                                      >  1:826669/1‑141 (MQ=255)                                                                       gAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGAt                                                                      <  1:910256/141‑1 (MQ=255)                                                                          ttattGCCCTTTGTCGTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGc                                                                   <  2:19029/141‑1 (MQ=255)                                                                            attGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCAt                                                                 <  2:739016/141‑1 (MQ=255)                                                                             ttGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCAtt                                                                <  2:608802/141‑1 (MQ=255)                                                                                      gTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTcc                                                       <  1:22713/141‑1 (MQ=255)                                                                                      gTCTTTAATGCGACGAACTAGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTcc                                                       <  1:555023/141‑1 (MQ=255)                                                                                         tttAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAAc                                                    <  2:228391/141‑1 (MQ=255)                                                                                         tttAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAAc                                                    >  1:280162/1‑141 (MQ=255)                                                                                         tttAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTAGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAAc                                                    >  1:799974/1‑141 (MQ=255)                                                                                           tAATGCGACGACCTGGACTCTCTTTAAATACAAACTTCACAACATCAATTGCACAAACTAATCCAACAACACTTGGCACCAAGGCGTTTGAAGACGGT‑‑GGCATTAATCATTTCCGACTGCTAGCATTTTTATCTCCAACta                                                  <  2:158433/141‑1 (MQ=255)                                                                                           tAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACta                                                  <  1:11528/141‑1 (MQ=255)                                                                                            aaTGCGACGAACTGGAATATCTTTTAATAAAACATGCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACtat                                                 >  1:845986/1‑141 (MQ=255)                                                                                                  acgaACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTTTATCttt                                           >  1:307247/1‑141 (MQ=255)                                                                                                  acgaACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCttt                                           >  1:724018/1‑141 (MQ=255)                                                                                                  acgaACTGGAATATCTTTTAATACGTCATTCACCACATAACTAGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACta                                                  >  2:150088/1‑134 (MQ=255)                                                                                                  acgaACTGGAATATCTTTTAATACGTCATTCACCACATAACTAGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACta                                                  <  1:150088/134‑1 (MQ=255)                                                                                                        tGGAATATCTTTTAATACGTCATTCACCACATAACTAGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACAtc                                     <  2:534752/141‑1 (MQ=255)                                                                                                             tatCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTtctc                                <  2:629672/141‑1 (MQ=255)                                                                                                              atCTTTTAATACGTCATTCACATCATAGCTTGCTCTAATATATCCAACAACACTTGGCACAAAGACAATTGATGAAGGT‑‑CGCATTTGTTCTTTTCGATTGATACCATTTTTAGCTCCAGCTACAACTTGTACATCATctct                               <  2:924944/141‑1 (MQ=255)                                                                                                               tCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCtt                              <  2:765736/141‑1 (MQ=255)                                                                                                                 ttttAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTAt                            >  1:493890/1‑141 (MQ=255)                                                                                                                  tttAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACCCTTGGCCCAAACGCATTTGAAGAAGGT‑‑GTCATTTGGCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATg                           <  1:486395/141‑1 (MQ=255)                                                                                                                   ttAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGa                          >  1:306037/1‑141 (MQ=255)                                                                                                                   ttAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGa                          >  2:38978/1‑141 (MQ=255)                                                                                                                   ttAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGa                          >  1:881259/1‑141 (MQ=255)                                                                                                                       tACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAAt                      <  2:629946/141‑1 (MQ=255)                                                                                                                       tACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAAt                      <  1:1002552/141‑1 (MQ=255)                                                                                                                         cGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTg                    <  2:1032449/141‑1 (MQ=255)                                                                                                                           tCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGa                  >  1:704969/1‑141 (MQ=255)                                                                                                                              ttCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACtt               <  2:280317/141‑1 (MQ=255)                                                                                                                                  ccacATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCAt           >  1:975567/1‑141 (MQ=255)                                                                                                                                     caTAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTg        <  2:327467/141‑1 (MQ=255)                                                                                                                                       tAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTGaa      <  1:590555/141‑1 (MQ=255)                                                                                                                                         aCTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTGaaaa    <  1:134366/141‑1 (MQ=255)                                                                                                                                          cTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTGAAAAt   <  2:383362/141‑1 (MQ=255)                                                                                                                                           ttGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTGAAAATa  <  2:111938/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                              CGAACTAGTCAGTTTTAGTTTTATGGTGTGGTTACACTTGCCTTACGTTGAAAATTATCCCTTTCAAAATGAATTATTGCCCTTTGTCTTTAATGCGACGAACTGGAATATCTTTTAATACGTCATTCACCACATAACTTGCACAAATTAATCCAACAACACTTGGCACAAAGGCATTTGAAGAAGGT‑‑GGCATTTGTCCTTTTCGATTGATAGCATTTTTATCTCCAACTATATCTTTTACATCTTCTCTTATGACAATTGGACTTTCATCTGAAAATA  >  CP000730/1735388‑1735666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑