Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,746,065 A→T 5.6% V301D (GTC→GAC)  recJ ← recombination protein RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,746,0650AT 5.6% 275.4 / 6.3 89V301D (GTC→GAC) recJrecombination protein RecJ
Reads supporting (aligned to +/- strand):  ref base A (38/46);  new base T (3/2);  total (41/48)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

TAAAAGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCAATGCTTTAACA  >  CP000730/1745926‑1746205
                                                                                                                                           |                                                                                                                                            
tAAAAGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGAc                                                                                                                                             <  1:612161/141‑1 (MQ=255)
tAAAAGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGAc                                                                                                                                             <  1:74521/141‑1 (MQ=255)
 aaaaGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACg                                                                                                                                            <  1:706394/141‑1 (MQ=255)
  aaaGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACgt                                                                                                                                           <  2:56992/141‑1 (MQ=255)
   aaGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACgtc                                                                                                                                          <  1:948387/141‑1 (MQ=255)
    aGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACgtca                                                                                                                                         >  1:49754/1‑141 (MQ=255)
     gTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACgtcag                                                                                                                                        <  2:638183/141‑1 (MQ=255)
        aaaaTAAATCTCCCTTTTTAATTTTTGTTTCTGCCATAGCAATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCTACgtcagtca                                                                                                                                     >  2:278984/1‑141 (MQ=255)
         aaataaatCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGtcat                                                                                                                                    <  1:643019/141‑1 (MQ=255)
         aaataaatCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGtcat                                                                                                                                    <  2:916019/141‑1 (MQ=255)
          aataaatCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGTCGTCAGtcatc                                                                                                                                   >  1:949839/1‑141 (MQ=255)
          aataaatCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGtcatc                                                                                                                                   <  1:869840/141‑1 (MQ=255)
          aataaatCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGtcatc                                                                                                                                   <  1:454326/141‑1 (MQ=255)
           ataaatCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGtcatca                                                                                                                                  >  1:136395/1‑141 (MQ=255)
                   cccTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCg                                                                                                                          >  2:945404/1‑141 (MQ=255)
                   cccTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCg                                                                                                                          >  2:879674/1‑141 (MQ=255)
                           aaCTTTTGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTaa                                                                                                                  >  2:895043/1‑141 (MQ=255)
                           aaCTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTaa                                                                                                                  >  2:949297/1‑141 (MQ=255)
                             cTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAatg                                                                                                                >  2:53351/1‑141 (MQ=255)
                                 cGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGc                                                                                                            <  1:696885/141‑1 (MQ=255)
                                   tttCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGt                                                                                                          <  2:54998/141‑1 (MQ=255)
                                     tCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCa                                                                                                        <  2:1084930/141‑1 (MQ=255)
                                      cTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCAt                                                                                                       <  2:770441/141‑1 (MQ=255)
                                        cccATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACTTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCt                                                                                                     <  2:275467/140‑1 (MQ=255)
                                                 aTTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCCCGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACCACCt                                                                                            >  2:749628/1‑141 (MQ=255)
                                                 aTTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCt                                                                                            >  2:175938/1‑141 (MQ=255)
                                                  ttGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGTCGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAACCGACCTa                                                                                           >  2:834377/1‑141 (MQ=255)
                                                  ttGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTa                                                                                           >  1:941156/1‑141 (MQ=255)
                                                  ttGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTa                                                                                           >  1:70557/1‑141 (MQ=255)
                                                     cttcttCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAg                                                                                        >  2:1017387/1‑141 (MQ=255)
                                                      ttcttcTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGc                                                                                       <  1:696020/141‑1 (MQ=255)
                                                      ttcttcTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGc                                                                                       >  2:942570/1‑141 (MQ=255)
                                                      ttcttcTGTGATAGTCGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGc                                                                                       <  1:603943/141‑1 (MQ=255)
                                                       tcttctGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCa                                                                                      <  2:189666/141‑1 (MQ=255)
                                                       tcttctGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCa                                                                                      <  2:189563/141‑1 (MQ=255)
                                                           ctGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTaa                                                                                  <  2:884352/141‑1 (MQ=255)
                                                            tgtgATAGTTGCTACAATAACTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAg                                                                                 <  2:641098/141‑1 (MQ=255)
                                                              tgATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTc                                                                               >  2:301961/1‑141 (MQ=255)
                                                              tgATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTc                                                                               >  1:611220/1‑141 (MQ=255)
                                                               gATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCt                                                                              <  1:698387/141‑1 (MQ=255)
                                                                 tAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAg                                                                            <  2:376059/141‑1 (MQ=255)
                                                                  aGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAgg                                                                           <  1:643370/141‑1 (MQ=255)
                                                                       cTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCaa                                                                      <  2:700758/141‑1 (MQ=255)
                                                                           aaTATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGTCGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCAGCTAAACGACCTACAGCATTAAGTCTAGGTCCaataat                                                                  >  1:826878/1‑141 (MQ=255)
                                                                              atCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATaaa                                                               <  2:179967/141‑1 (MQ=255)
                                                                                 ttttctttCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAAcc                                                            >  2:580032/1‑141 (MQ=255)
                                                                                 ttttctttCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAAcc                                                            <  1:231552/141‑1 (MQ=255)
                                                                                 ttttctttCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAAcc                                                            >  2:655505/1‑141 (MQ=255)
                                                                                   ttctttcACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCaa                                                          <  2:390436/141‑1 (MQ=255)
                                                                                   ttctttcACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCaa                                                          <  1:268186/141‑1 (MQ=255)
                                                                                    tctttcACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAAt                                                         >  2:1012912/1‑141 (MQ=255)
                                                                                    tctttcACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAAt                                                         <  1:709409/141‑1 (MQ=255)
                                                                                    tctttcACGGTTAAAATGTCCAACTTGTTCTGCCAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGCCCTACAGCACTAAGTCTAGGTCCAATAATAAAACCact                                                         >  2:695091/1‑139 (MQ=255)
                                                                                      tttcACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTg                                                       <  1:942570/141‑1 (MQ=255)
                                                                                       ttcACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGt                                                      <  1:451230/141‑1 (MQ=255)
                                                                                          aCGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTttc                                                   >  1:796114/1‑141 (MQ=255)
                                                                                           cGGTTAAAATGTTCAACTTGTTCTGCTAAAACTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTttct                                                  >  1:29194/1‑141 (MQ=255)
                                                                                            ggTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTttctt                                                 >  1:763064/1‑141 (MQ=255)
                                                                                              ttAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTtca                                               <  1:301961/141‑1 (MQ=255)
                                                                                               tAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTtcat                                              >  2:468492/1‑141 (MQ=255)
                                                                                                   aTGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAat                                          <  1:101533/141‑1 (MQ=255)
                                                                                                   aTGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAat                                          >  1:379889/1‑141 (MQ=255)
                                                                                                     gTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGTCGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAatat                                        <  1:134220/141‑1 (MQ=255)
                                                                                                      ttCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATAtt                                       >  2:4397/1‑141 (MQ=255)
                                                                                                        cAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGt                                     >  2:162227/1‑141 (MQ=255)
                                                                                                        cAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGt                                     <  1:648940/141‑1 (MQ=255)
                                                                                                          aCTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCa                                   <  1:478202/141‑1 (MQ=255)
                                                                                                                  tGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATcc                           >  2:800095/1‑141 (MQ=255)
                                                                                                                  tGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATcc                           <  2:734765/141‑1 (MQ=255)
                                                                                                                    cTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCg                         <  1:400688/141‑1 (MQ=255)
                                                                                                                      aaaaaTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGct                       >  2:1046225/1‑141 (MQ=255)
                                                                                                                       aaaaTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGctt                      <  2:1014035/141‑1 (MQ=255)
                                                                                                                       aaaaTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGctt                      >  1:297619/1‑141 (MQ=255)
                                                                                                                        aaaTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGcttc                     <  2:796114/141‑1 (MQ=255)
                                                                                                                         aaTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGcttct                    <  2:116522/141‑1 (MQ=255)
                                                                                                                          aTGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGcttctt                   <  1:242894/141‑1 (MQ=255)
                                                                                                                             cAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTa                <  2:298185/141‑1 (MQ=255)
                                                                                                                             cAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAACATTGTCATTGTATCCCGCTTCTTTTa                >  2:861228/1‑141 (MQ=255)
                                                                                                                             cAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGACATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTa                >  2:148967/1‑141 (MQ=255)
                                                                                                                              aGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTACTGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAg               <  2:237835/141‑1 (MQ=255)
                                                                                                                              aGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAg               >  1:286231/1‑141 (MQ=255)
                                                                                                                              aGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATGGTCATTGTATCCCGCTTCTTTTAg               <  2:238026/141‑1 (MQ=255)
                                                                                                                              aGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATCGTTTCTTCATCAATATTGTCATTGTATCCAGCTTCTTTTAg               >  1:47992/1‑141 (MQ=255)
                                                                                                                               gCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGc              >  2:889631/1‑141 (MQ=255)
                                                                                                                                cttcttcttCGTCGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCa             <  2:773146/141‑1 (MQ=255)
                                                                                                                                cttcttcttCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCa             >  2:674471/1‑141 (MQ=255)
                                                                                                                                     tcttcGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCAATGCt        >  1:833770/1‑141 (MQ=255)
                                                                                                                                     tcttcGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCAATGCt        <  1:105370/141‑1 (MQ=255)
                                                                                                                                       ttcGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCATTGCttt      >  1:660253/1‑141 (MQ=255)
                                                                                                                                           aCGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCAATGCTTTAACa  <  1:315165/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAAAAGTAAAAATAAATCTCCCTTTTTAACTTTCGTTTCTGCCATAGCCATTGCTTCTTCTGTGATAGTTGCTACAATATCTTTTCTTTCACGGTTAAAATGTTCAACTTGTTCTGCTAAAAATGCAGCTTCTTCTTCGACGTCAGTCATCAACAATTCGCAAGCTAATGATGCGTCATCTAAACGACCTACAGCATTAAGTCTAGGTCCAATAATAAAACCAATTGTTTCTTCATCAATATTGTCATTGTATCCCGCTTCTTTTAGCAATGCTTTAACA  >  CP000730/1745926‑1746205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: