Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,749,170 T→A 5.1% V93V (GTA→GTT secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,749,1700TA 5.1% 241.2 / 4.9 79V93V (GTA→GTTsecDFRND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (48/27);  new base A (2/2);  total (50/29)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

TAAATTCTTGTTTGGCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTG  >  CP000730/1749031‑1749310
                                                                                                                                           |                                                                                                                                            
tAAATTCTTGTTTGGCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTa                                                                                                                                             >  2:825166/1‑141 (MQ=255)
tAAATTCTTGTTTGGCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTa                                                                                                                                             >  2:402253/1‑141 (MQ=255)
   aTTCTTGTTTGGCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGAGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTActc                                                                                                                                          >  2:316844/1‑141 (MQ=255)
      cTTGTTTGGCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTaa                                                                                                                                       <  1:495664/141‑1 (MQ=255)
             ggCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTAtt                                                                                                                                <  2:331746/141‑1 (MQ=255)
               cAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAACTCTATTAg                                                                                                                              >  2:888523/1‑141 (MQ=255)
                aGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTCTTAgg                                                                                                                             >  2:304788/1‑141 (MQ=255)
                       tGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGACCTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTCTTACACCAGCAAGTTTTACTCTAATTCTATTAGGTTCTTCa                                                                                                                      >  1:255223/1‑141 (MQ=255)
                         tttAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAAc                                                                                                                    <  2:57690/141‑1 (MQ=255)
                          ttAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACt                                                                                                                   <  2:922762/141‑1 (MQ=255)
                             atatCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGa                                                                                                                >  2:757450/1‑141 (MQ=255)
                               atCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAAt                                                                                                              >  2:773356/1‑141 (MQ=255)
                               atCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAAt                                                                                                              >  1:763892/1‑141 (MQ=255)
                               atCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACATGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAAt                                                                                                              <  2:324112/141‑1 (MQ=255)
                                     aCCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAgg                                                                                                        >  2:464904/1‑141 (MQ=255)
                                      ccGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGt                                                                                                       >  2:378058/1‑141 (MQ=255)
                                       cGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGtt                                                                                                      <  2:975470/141‑1 (MQ=255)
                                                ttACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAacac                                                                                             <  2:602168/141‑1 (MQ=255)
                                                 tACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACAcc                                                                                            <  2:596704/141‑1 (MQ=255)
                                                  aCTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCt                                                                                           >  1:379273/1‑141 (MQ=255)
                                                   cTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTa                                                                                          >  1:480596/1‑141 (MQ=255)
                                                        tcttctGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACg                                                                                     <  2:108927/141‑1 (MQ=255)
                                                        tcttctGCATCGCGAATTGTTAAATCAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGGTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACg                                                                                     >  1:183109/1‑141 (MQ=255)
                                                            ctGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTaa                                                                                 >  2:76910/1‑141 (MQ=255)
                                                               cATCGCGAATTGTTAAAGTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAacac                                                                              >  2:248540/1‑141 (MQ=255)
                                                                   gcgAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAACACGTTAACCCGCtt                                                                          >  2:596468/1‑141 (MQ=255)
                                                                   gcgAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGAtt                                                                          <  1:281707/141‑1 (MQ=255)
                                                                    cgAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGAttt                                                                         >  1:894435/1‑141 (MQ=255)
                                                                       aTTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCt                                                                      <  1:1053589/141‑1 (MQ=255)
                                                                       aTTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCt                                                                      >  1:291624/1‑141 (MQ=255)
                                                                        ttGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTa                                                                     >  1:398173/1‑141 (MQ=255)
                                                                        ttGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTa                                                                     >  1:396400/1‑141 (MQ=255)
                                                                        ttGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTa                                                                     <  1:1065321/141‑1 (MQ=255)
                                                                        ttGTTAAATTAGCTGGCGAAGATAAACTTTTACGTGCTTCATTCTGGTCTGTTACGCCAGCAGGTTGTACTCTAATTCTAGTAGGCTCTTCAACTTGAATGTTAGGTTCCGACACACCAAAACCATTAccacactttcggg                                                                     >  2:91866/1‑132 (MQ=255)
                                                                         tGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTaa                                                                    >  2:1057293/1‑141 (MQ=255)
                                                                          gTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAACCGTTAACACGATTTTTTAAt                                                                   >  2:856384/1‑141 (MQ=255)
                                                                           ttAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATg                                                                  >  1:870342/1‑141 (MQ=255)
                                                                            tAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACAAGATTTTCTAATGt                                                                 >  1:710075/1‑141 (MQ=255)
                                                                                    cTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTCATCTCTGCGCtt                                                         >  2:585565/1‑140 (MQ=255)
                                                                                        agaagaTAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGa                                                     >  1:151016/1‑141 (MQ=255)
                                                                                           agaTAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTg                                                  <  1:204456/141‑1 (MQ=255)
                                                                                            gaTAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGt                                                 >  1:837654/1‑141 (MQ=255)
                                                                                              tAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTaa                                               <  1:885622/141‑1 (MQ=255)
                                                                                                 aaTTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGc                                            <  1:1049709/141‑1 (MQ=255)
                                                                                                  aTTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCt                                           >  2:361687/1‑141 (MQ=255)
                                                                                                  aTTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCt                                           <  1:645823/141‑1 (MQ=255)
                                                                                                   ttttACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtt                                          >  1:578311/1‑141 (MQ=255)
                                                                                                    tttACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCttt                                         >  2:111272/1‑141 (MQ=255)
                                                                                                     ttACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtttt                                        >  2:688797/1‑141 (MQ=255)
                                                                                                     ttACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtttt                                        >  1:751912/1‑141 (MQ=255)
                                                                                                     ttACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtttt                                        <  1:1608/141‑1 (MQ=255)
                                                                                                     ttACGGGCTTCATTCGGGCCTGTTACACCAGCAAGTTTTACTCTAATTCTGGCAGGGTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCGTTTGATTGTATGGCtttt                                        >  2:918409/1‑141 (MQ=255)
                                                                                                       aCGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtttttt                                      >  1:418137/1‑141 (MQ=255)
                                                                                                       aCGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtttttt                                      <  2:717230/141‑1 (MQ=255)
                                                                                                       aCGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCtttttt                                      >  2:100941/1‑141 (MQ=255)
                                                                                                        cGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCttttttt                                     >  2:878681/1‑140 (MQ=255)
                                                                                                        cGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTa                                     <  2:705276/141‑1 (MQ=255)
                                                                                                            cTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTa                                 >  2:717504/1‑141 (MQ=255)
                                                                                                            cTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTa                                 >  2:717575/1‑141 (MQ=255)
                                                                                                              tCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTAtt                               <  1:86919/141‑1 (MQ=255)
                                                                                                                    tGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTTTCTTTTTTGTCg                         >  2:624506/1‑141 (MQ=255)
                                                                                                                    tGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCg                         >  1:100160/1‑141 (MQ=255)
                                                                                                                     ggTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGc                        <  2:905647/141‑1 (MQ=255)
                                                                                                                     ggTCTGTTACACCAGCAAGTTGAACTCTAATTCTATTAGGtt                                                                                                                           >  2:230956/1‑42 (MQ=255)
                                                                                                                     ggTCTGTTACACCAGCAAGTTGAACTCTAATTCTATTAGGtt                                                                                                                           <  1:230956/42‑1 (MQ=255)
                                                                                                                      gTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGcc                       >  2:820681/1‑141 (MQ=255)
                                                                                                                       tCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCt                      <  2:713022/141‑1 (MQ=255)
                                                                                                                        cTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCtt                     <  1:367065/141‑1 (MQ=255)
                                                                                                                        cTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTGCCTAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCtt                     <  1:43169/141‑1 (MQ=255)
                                                                                                                         tGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCttt                    >  1:264854/1‑141 (MQ=255)
                                                                                                                            tACACCAGCAAGTTGAACTCTAATTCTATTAGGTTCTTCAACt                                                                                                                   >  2:399016/1‑43 (MQ=255)
                                                                                                                            tACACCAGCAAGTTGAACTCTAATTCTATTAGGTTCTTCAACt                                                                                                                   <  1:399016/43‑1 (MQ=255)
                                                                                                                             acacCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCtttattt                >  1:306908/1‑141 (MQ=255)
                                                                                                                                  aGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAgg           >  1:963356/1‑141 (MQ=255)
                                                                                                                                  aGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAAATTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAgg           <  1:802365/141‑1 (MQ=255)
                                                                                                                                        ttGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGAc     >  2:735250/1‑141 (MQ=255)
                                                                                                                                        ttGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGAc     >  1:165217/1‑141 (MQ=255)
                                                                                                                                         tGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACt    <  1:825166/141‑1 (MQ=255)
                                                                                                                                         tGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACt    <  2:379273/141‑1 (MQ=255)
                                                                                                                                           tACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTg  <  1:757450/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAAATTCTTGTTTGGCAGATCCCTGTTTAATATCAGAACCGCTTAATTTTACTTTATCTTCTGCATCGCGAATTGTTAAATTAGCTTGAGAAGATAAAATTTTACGTGCTTCATTCTGGTCTGTTACACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTG  >  CP000730/1749031‑1749310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: