Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,868,200 C→T 5.1% V132I (GTT→ATT)  ytnP ← possible metal‑dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,868,2000CT 5.1% 258.8 / 2.2 81V132I (GTT→ATT) ytnPpossible metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base C (41/35);  new base T (2/2);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

CTTGATACCCAGAACCGGTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATTATATGAC  >  CP000730/1868060‑1868340
                                                                                                                                            |                                                                                                                                            
cTTGATACCCAGAACCGGTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCacaac                                                                                                                                              <  1:765595/141‑1 (MQ=255)
   gagaCCCAGAACCGGTTCAAAATGGTTTTCGAATAAAATCAACTTGGTACTATAATCTCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCGTGTTGCacaacacg                                                                                                                                           >  2:647705/4‑139 (MQ=255)
   gATACCCAGAACCGGTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATg                                                                                                                                           >  1:416907/1‑141 (MQ=255)
     tACCCAGAACCGGTTCAAAATGTTTTTCGAATAAAATGAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGaa                                                                                                                                         >  1:782554/1‑141 (MQ=255)
          aGAACCGGTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCa                                                                                                                                    >  2:293141/1‑141 (MQ=255)
               cGGTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTc                                                                                                                               >  1:796687/1‑141 (MQ=255)
                ggTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCa                                                                                                                              <  2:237713/141‑1 (MQ=255)
                 gTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTTCACAACATGAATCGCATTTTCaa                                                                                                                             >  2:271077/1‑141 (MQ=255)
                 gTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCaa                                                                                                                             >  1:279650/1‑141 (MQ=255)
                 gTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCaa                                                                                                                             >  2:213390/1‑141 (MQ=255)
                 gTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCaa                                                                                                                             <  1:733119/141‑1 (MQ=255)
                    cAAAATGGTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGAGTTACTTCTTATATGATGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATAGAAATTTCaaaaa                                                                                                                          <  2:47868/141‑1 (MQ=255)
                     aaaaTGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAAt                                                                                                                         >  2:798189/1‑141 (MQ=255)
                     aaaaTGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAAt                                                                                                                         <  1:167299/141‑1 (MQ=255)
                       aaTGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTg                                                                                                                       <  2:696677/141‑1 (MQ=255)
                        aTGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGc                                                                                                                      <  1:351196/141‑1 (MQ=255)
                         tGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCCGTGATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCa                                                                                                                     <  1:1062973/141‑1 (MQ=255)
                               tCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTccc                                                                                                               >  1:542969/1‑140 (MQ=255)
                               tCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCt                                                                                                               <  1:165391/141‑1 (MQ=255)
                                cGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTg                                                                                                              >  2:219383/1‑141 (MQ=255)
                                cGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCGTATTTCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTg                                                                                                              >  1:293431/1‑141 (MQ=255)
                                      ttttCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAATCTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGAt                                                                                                        >  2:11937/4‑141 (MQ=255)
                                      aaaTCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGAt                                                                                                        <  2:1971/141‑1 (MQ=255)
                                       aaTCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATc                                                                                                       <  2:741054/141‑1 (MQ=255)
                                          cAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCCGt                                                                                                    >  2:686279/1‑141 (MQ=255)
                                          cAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGt                                                                                                    >  1:839524/1‑141 (MQ=255)
                                           aaCTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTc                                                                                                   >  2:205160/1‑141 (MQ=255)
                                           aaCTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTc                                                                                                   <  2:169830/141‑1 (MQ=255)
                                           aaCTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTc                                                                                                   <  1:829143/141‑1 (MQ=255)
                                           aaCTTGTTACTAGAATCGCCTTTATACTTAAACCAGCAAGTGGTTTTGCTGCCAATATTAGTTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCAGTATTTAAAAATGGATGTCGTGCtgnaacgggc                                                                                                   >  2:812868/1‑132 (MQ=255)
                                                     tataATAGCATTTATTCTTATCCCAGTAAGTTGATTTACTTATTATATTAAGTGCAATAAACTCATGCCACTTATCTTGTTGCACAAGATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTTAAAACGgcag                                                                                         <  2:51946/141‑1 (MQ=255)
                                                       taATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGgcagca                                                                                       >  1:624898/1‑141 (MQ=255)
                                                        aaTCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCAt                                                                                      <  1:1015131/141‑1 (MQ=255)
                                                        aaTCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCAt                                                                                      <  2:254620/141‑1 (MQ=255)
                                                          tCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGa                                                                                    <  2:602142/141‑1 (MQ=255)
                                                            gCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATc                                                                                  <  2:452136/141‑1 (MQ=255)
                                                              cTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCCGCTTGATCAGTCAAACCGGCAGCATGATCaa                                                                                <  1:804140/141‑1 (MQ=255)
                                                              cTTTATTCTTATCCAAGTAAGTTAATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGTCAGCATGATCaa                                                                                <  2:213101/141‑1 (MQ=255)
                                                                     cTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCa                                                                                                                              >  1:824454/1‑88 (MQ=255)
                                                                     cTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCa                                                                                                                              <  2:824454/88‑1 (MQ=255)
                                                                     cTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCat                                                                         <  1:512339/141‑1 (MQ=255)
                                                                      ttATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCata                                                                        >  2:978736/1‑141 (MQ=255)
                                                                         tCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATAtgt                                                                     <  1:494849/141‑1 (MQ=255)
                                                                            cAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTc                                                                  >  1:740811/1‑141 (MQ=255)
                                                                                aaGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTa                                                              <  2:131228/141‑1 (MQ=255)
                                                                                 aGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAg                                                             >  1:970440/1‑141 (MQ=255)
                                                                                    tGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGcac                                                          >  2:124441/1‑141 (MQ=255)
                                                                                       tttACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATa                                                       >  1:883080/1‑141 (MQ=255)
                                                                                          aCTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATc                                                    <  1:61049/141‑1 (MQ=255)
                                                                                            ttcttATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCaa                                                  <  2:43445/141‑1 (MQ=255)
                                                                                            ttcttATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCaa                                                  <  2:511656/141‑1 (MQ=255)
                                                                                              cttATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAata                                                >  2:620641/1‑141 (MQ=255)
                                                                                              cttATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAata                                                >  2:831462/1‑141 (MQ=255)
                                                                                                 atatTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATcc                                             >  2:55216/1‑141 (MQ=255)
                                                                                                   atTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCtt                                           <  1:514420/141‑1 (MQ=255)
                                                                                                    ttAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCttt                                          >  1:1070221/1‑141 (MQ=255)
                                                                                                     tAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTg                                         >  1:875998/1‑141 (MQ=255)
                                                                                                          gCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGtt                                    >  2:1013276/1‑141 (MQ=255)
                                                                                                            aaTAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTaa                                  >  1:1063685/1‑141 (MQ=255)
                                                                                                             aTAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTaaa                                 >  1:833496/1‑141 (MQ=255)
                                                                                                             aTAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTaaa                                 <  2:119597/141‑1 (MQ=255)
                                                                                                             aTAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTaaa                                 >  2:1075427/1‑141 (MQ=255)
                                                                                                             aTAAACTCATGCCACTCATCTTGTTGCACAACATGAAGCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCGGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTaaa                                 <  2:658852/141‑1 (MQ=255)
                                                                                                                aaCTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATta                              >  2:1054639/1‑141 (MQ=255)
                                                                                                                 aCTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTCGCACATAATCAATATCCTTTGGCGTTAAATtat                             >  2:1027922/1‑141 (MQ=255)
                                                                                                                    cATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAAt                          >  1:941393/1‑141 (MQ=255)
                                                                                                                     aTGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAAtt                         >  1:437679/1‑141 (MQ=255)
                                                                                                                     aTGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAAtt                         >  2:896605/1‑141 (MQ=255)
                                                                                                                      tGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAAttt                        <  1:703214/141‑1 (MQ=255)
                                                                                                                      tGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAAttt                        <  2:782554/141‑1 (MQ=255)
                                                                                                                      tGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAAttt                        >  1:607363/1‑141 (MQ=255)
                                                                                                                       gCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTg                       >  1:888021/1‑141 (MQ=255)
                                                                                                                               tCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCa               <  1:201023/141‑1 (MQ=255)
                                                                                                                               tCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTAATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCa               <  1:942400/141‑1 (MQ=255)
                                                                                                                                cTTGTTGCACAATATGAATCGCATTTTCAAAAATTGc                                                                                                                      >  1:358698/1‑37 (MQ=255)
                                                                                                                                cTTGTTGCACAATATGAATCGCATTTTCAAAAATTGc                                                                                                                      >  1:358960/1‑37 (MQ=255)
                                                                                                                                cTTGTTGCACAATATGAATCGCATTTTCAAAAATTGc                                                                                                                      <  2:358698/37‑1 (MQ=255)
                                                                                                                                cTTGTTGCACAATATGAATCGCATTTTCAAAAATTGc                                                                                                                      <  2:358960/37‑1 (MQ=255)
                                                                                                                                cTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAg              <  1:498050/141‑1 (MQ=255)
                                                                                                                                cTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAg              <  2:1015991/141‑1 (MQ=255)
                                                                                                                                cTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAg              <  1:941231/141‑1 (MQ=255)
                                                                                                                                   gttgCACAACATGAATCGCATTTTCAAAAATTGCATGTTCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCaa           >  1:285529/1‑141 (MQ=255)
                                                                                                                                       cacaACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATtat       >  2:672617/1‑141 (MQ=255)
                                                                                                                                        acaacaTGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATtata      <  1:196032/141‑1 (MQ=255)
                                                                                                                                         caacaTGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATTatat     >  1:253783/1‑141 (MQ=255)
                                                                                                                                         caacaTGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATTatat     >  1:983005/1‑141 (MQ=255)
                                                                                                                                            caTGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATTATATGAc  <  1:290487/141‑1 (MQ=255)
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CTTGATACCCAGAACCGGTTCAAAATGTTTTTCGAATAAAATCAACTTGTTACTATAATCGCCTTTATTCTTATCCCAGTAAGTTGATTTACTTCTTATATTAGGTGCAATAAACTCATGCCACTCATCTTGTTGCACAACATGAATCGCATTTTCAAAAATTGCATGTCCTGCTTGATCAGTCAAACCGGCAGCATGATCAAAATGCATATGTGTCATTAGCACATAATCAATATCCTTTGGCGTTAAATTATAATTTGCCAAATCAGCAATTATATGAC  >  CP000730/1868060‑1868340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: