Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,970,814 G→C 5.1% intergenic (+3/‑730) USA300HOU_1831 → / → USA300HOU_1832 hypothetical protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,970,8140GC 5.1% 307.6 / 5.7 92intergenic (+3/‑730)USA300HOU_1831/USA300HOU_1832hypothetical protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (46/41);  new base C (2/3);  total (48/44)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATTGGTGGTGAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTA  >  CP000730/1970678‑1970954
                                                                                                                                        |                                                                                                                                            
cAATTGGTGGTGAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTa                                                                                                                                          >  2:1086600/1‑141 (MQ=255)
  aTTGGTGGTGAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACg                                                                                                                                        >  1:459412/1‑141 (MQ=255)
   ttGGTGGTGAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGt                                                                                                                                       <  1:955517/141‑1 (MQ=255)
    tggtggTGAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGtt                                                                                                                                      >  2:519817/1‑141 (MQ=255)
       tggtgAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTtctc                                                                                                                                   >  2:384344/1‑141 (MQ=255)
       tggtgAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTtctc                                                                                                                                   >  1:925953/1‑141 (MQ=255)
       tggtgAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTtctc                                                                                                                                   >  1:292626/1‑141 (MQ=255)
        ggtgAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCg                                                                                                                                  <  2:673434/141‑1 (MQ=255)
          tgAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCCAAGTAGCAGGTTACGTTCTCGAt                                                                                                                                >  2:318007/1‑141 (MQ=255)
            actttAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATca                                                                                                                              <  1:1050698/138‑1 (MQ=255)
            actttAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATca                                                                                                                              <  1:845288/138‑1 (MQ=255)
             aaTTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATcac                                                                                                                             <  1:486288/141‑1 (MQ=255)
              aTTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATcaca                                                                                                                            <  2:52270/141‑1 (MQ=255)
              aTTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATcaca                                                                                                                            <  2:511789/141‑1 (MQ=255)
               ttAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACaa                                                                                                                           >  2:12344/1‑141 (MQ=255)
                tAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAAc                                                                                                                          <  2:519642/141‑1 (MQ=255)
                 aaGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACt                                                                                                                         >  1:922113/1‑141 (MQ=255)
                  aGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTa                                                                                                                        <  2:677237/141‑1 (MQ=255)
                    tCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTAtt                                                                                                                      <  2:579673/141‑1 (MQ=255)
                       ttACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTAttttt                                                                                                                   >  1:959343/1‑141 (MQ=255)
                       ttACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTAttttt                                                                                                                   <  1:513794/141‑1 (MQ=255)
                       ttACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGGGGCTAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTAttttt                                                                                                                   <  2:974015/141‑1 (MQ=255)
                        tACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTtta                                                                                                                  <  2:769306/141‑1 (MQ=255)
                           ttGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTttatta                                                                                                               >  1:297635/1‑141 (MQ=255)
                           ttGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTttatta                                                                                                               >  2:450303/1‑141 (MQ=255)
                            tGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAg                                                                                                              <  2:504841/141‑1 (MQ=255)
                             ggAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGt                                                                                                             >  1:825450/1‑141 (MQ=255)
                             ggAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGt                                                                                                             >  2:235375/1‑141 (MQ=255)
                              gAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTa                                                                                                            >  2:1011298/1‑141 (MQ=255)
                                    tCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCa                                                                                                      >  2:47646/1‑141 (MQ=255)
                                    tCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCa                                                                                                      >  1:616233/1‑141 (MQ=255)
                                     cAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGACACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCat                                                                                                     >  2:1053993/1‑141 (MQ=255)
                                      aaaTCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCata                                                                                                    >  2:257006/1‑141 (MQ=255)
                                         tCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAAGCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCAtattt                                                                                                 >  2:1002034/1‑141 (MQ=255)
                                          cTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCAtattta                                                                                                <  1:256436/141‑1 (MQ=255)
                                           tGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCAtatttat                                                                                               >  2:260302/1‑141 (MQ=255)
                                            gACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCACGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCAtatttatt                                                                                              >  1:94253/1‑141 (MQ=255)
                                             aCATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATatttattt                                                                                             >  2:168836/1‑141 (MQ=255)
                                              cATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTAtttt                                                                                            >  2:322084/1‑141 (MQ=255)
                                               aTTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGACCACAACTATTTTTATTAGTAACAGCATATTTAttttt                                                                                           <  1:786081/141‑1 (MQ=255)
                                                ttAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAACACGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTAtttttt                                                                                          <  1:254584/141‑1 (MQ=255)
                                                ttAATCCTAATATTGAACGTTTACAGTCACACATCAAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTAtttttt                                                                                          <  2:246677/141‑1 (MQ=255)
                                                     ccTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAAt                                                                                     >  1:31345/1‑141 (MQ=255)
                                                            ttGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGc                                                                              <  1:240601/141‑1 (MQ=255)
                                                            ttGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGc                                                                              <  2:119891/141‑1 (MQ=255)
                                                              gAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGACCCGAAACTTCTAAGTAGCACGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCa                                                                            <  1:918290/141‑1 (MQ=255)
                                                              gAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCa                                                                            <  2:241712/141‑1 (MQ=255)
                                                                aCGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCaaa                                                                          >  1:789106/1‑141 (MQ=255)
                                                                 cGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAAt                                                                         <  2:925953/141‑1 (MQ=255)
                                                                  gTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATa                                                                        >  1:896368/1‑141 (MQ=255)
                                                                     tACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAAc                                                                     >  2:555437/1‑141 (MQ=255)
                                                                       cAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACga                                                                   <  1:666774/141‑1 (MQ=255)
                                                                         gTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACgaga                                                                 >  2:288350/1‑141 (MQ=255)
                                                                           cacacaTCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATg                                                               >  1:374046/1‑141 (MQ=255)
                                                                               caTCGAAAAATTACAAAATCGTGGCTAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATCTTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACAt                                                           <  2:54033/141‑1 (MQ=255)
                                                                                 tCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTa                                                         <  1:878555/141‑1 (MQ=255)
                                                                                 tCGAAAANTTACAAAAGCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCACGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTTAAATTAAATGCCAAATAAACGAGATGACATTa                                                         <  2:815674/141‑1 (MQ=255)
                                                                                     aaaTTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGaaa                                                     >  1:340061/1‑141 (MQ=255)
                                                                                       aTTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACaagaaatatc                                                   <  1:151722/141‑10 (MQ=255)
                                                                                       aTTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAAtt                                                   >  1:775597/1‑141 (MQ=255)
                                                                                         ttACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAg                                                 <  2:83371/141‑1 (MQ=255)
                                                                                          tACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGa                                                >  2:873487/1‑141 (MQ=255)
                                                                                          tACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGa                                                >  1:842328/1‑141 (MQ=255)
                                                                                          tACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGa                                                <  2:129258/141‑1 (MQ=255)
                                                                                           aCAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGat                                               >  1:24432/1‑141 (MQ=255)
                                                                                            cAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGata                                              <  1:16051/141‑1 (MQ=255)
                                                                                            cAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGata                                              <  2:320216/141‑1 (MQ=255)
                                                                                            cAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGata                                              >  2:507872/1‑141 (MQ=255)
                                                                                              aaaTCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATAtt                                            >  2:10421/1‑141 (MQ=255)
                                                                                                   gTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTg                                       <  2:696256/141‑1 (MQ=255)
                                                                                                    tGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGt                                      <  2:81268/141‑1 (MQ=255)
                                                                                                     ggCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTc                                     <  2:635551/141‑1 (MQ=255)
                                                                                                     ggCGAGGATATTGCAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTc                                     <  2:720108/141‑1 (MQ=255)
                                                                                                      gCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCa                                    <  1:507872/141‑1 (MQ=255)
                                                                                                      gCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAAGTAGATATTTCTTGTCa                                    >  1:259415/1‑141 (MQ=255)
                                                                                                        gAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCAtc                                  <  1:168836/141‑1 (MQ=255)
                                                                                                            atatTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCtt                              >  2:767512/1‑141 (MQ=255)
                                                                                                              atTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCtttt                            <  1:229632/141‑1 (MQ=255)
                                                                                                                tGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCtttttt                          >  2:683148/1‑141 (MQ=255)
                                                                                                                 ggAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTa                         >  1:685135/1‑141 (MQ=255)
                                                                                                                  gAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTaa                        >  2:1033358/1‑141 (MQ=255)
                                                                                                                   aaaCGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTaaa                       >  2:186313/1‑141 (MQ=255)
                                                                                                                     aCGAAATTTCTAAGTAGCAGGTTACGTTCTCGTTCACAACTGTTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAAc                     <  2:180234/141‑1 (MQ=255)
                                                                                                                     aCGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAAc                     <  1:427019/141‑1 (MQ=255)
                                                                                                                     aCGAAACGTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAAc                     <  2:365511/141‑1 (MQ=255)
                                                                                                                      cGAAATTTCTAAGTAGCACGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTTAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTATAACt                    >  1:338895/1‑141 (MQ=255)
                                                                                                                        aaaTTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCa                  >  1:339394/1‑141 (MQ=255)
                                                                                                                        aaaTTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCa                  <  1:1018265/141‑1 (MQ=255)
                                                                                                                         aaTTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTGTTTTTTAAAACTCaa                 >  1:900274/1‑141 (MQ=255)
                                                                                                                               tAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAAc           >  2:1025130/1‑141 (MQ=255)
                                                                                                                                 aGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACtt         >  2:341567/1‑141 (MQ=255)
                                                                                                                                       aGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGttt   <  1:1027473/141‑1 (MQ=255)
                                                                                                                                        ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa  >  1:922924/1‑141 (MQ=255)
                                                                                                                                        ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa  >  1:109371/1‑141 (MQ=255)
                                                                                                                                        ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa  <  2:642984/141‑1 (MQ=255)
                                                                                                                                        ggTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTa  >  1:176913/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                            
CAATTGGTGGTGAAATTAAGTCATTACTTGGAAACTTCAAATCTGACATTAATCCTAATATTGAACGTTTACAGTCACACATCGAAAATTTACAAAATCGTGGCGAGGATATTGGAAACGAAATTTCTAAGTAGCAGGTTACGTTCTCGATCACAACTATTTTTATTAGTAACAGCATATTTATTTTTTAAAATTAAATGCCAAATAAACGAGATGACATTAGAAATTAGATATTTCTTGTCATCTCTTTTTTAAAACTCAAATGAACTTATGTTTA  >  CP000730/1970678‑1970954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: