Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,110,877 G→A 7.3% intergenic (‑123/+109) USA300HOU_1973 ← / ← USA300HOU_1974 hypothetical protein/hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,110,8770GA 7.3% 330.4 / 10.8 109intergenic (‑123/+109)USA300HOU_1973/USA300HOU_1974hypothetical protein/hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base G (57/44);  new base A (2/6);  total (59/50)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 112 total aligned reads displayed.

TTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTAT  >  CP000730/2110737‑2111017
                                                                                                                                            |                                                                                                                                            
ttCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAATGACACATCACATAGTg                                                                                                                                              <  2:75778/141‑1 (MQ=255)
ttCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTg                                                                                                                                              >  1:23120/1‑141 (MQ=255)
    tCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATgc                                                                                                                                          <  2:908342/141‑1 (MQ=255)
     cTTTAGTTATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTGGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATTCCTCATAAATAAAAAGACACATCACTATGTGATgtg                                                                                                                                         <  2:830191/141‑3 (MQ=255)
       ttAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGcc                                                                                                                                       >  2:332362/1‑141 (MQ=255)
        tAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCct                                                                                                                                      >  1:136485/1‑141 (MQ=255)
        tAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCct                                                                                                                                      <  1:107850/141‑1 (MQ=255)
        tAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCct                                                                                                                                      >  2:665378/1‑141 (MQ=255)
        tAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCct                                                                                                                                      >  1:960881/1‑141 (MQ=255)
        tAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCct                                                                                                                                      <  1:721332/141‑1 (MQ=255)
            catcatAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTg                                                                                                                                  >  2:351652/1‑141 (MQ=255)
             atcatAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGt                                                                                                                                 >  2:348353/1‑141 (MQ=255)
             atcatAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGt                                                                                                                                 <  2:189366/141‑1 (MQ=255)
               catAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTc                                                                                                                               >  1:618127/1‑141 (MQ=255)
                atAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCa                                                                                                                              <  1:419203/141‑1 (MQ=255)
                    tGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGcg                                                                                                                          <  2:239447/141‑1 (MQ=255)
                      tttCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATATTGATGCGCCTCTTGTTNATGcgtc                                                                                                                        >  2:812738/1‑141 (MQ=255)
                      tttCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGcgtc                                                                                                                        >  2:1020998/1‑141 (MQ=255)
                       ttCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGcgtcg                                                                                                                       >  1:165940/1‑141 (MQ=255)
                         cACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTa                                                                                                                     >  2:1062262/1‑141 (MQ=255)
                          aCCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTAt                                                                                                                    >  1:503770/1‑141 (MQ=255)
                          aCCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTAt                                                                                                                    >  2:537280/1‑141 (MQ=255)
                           ccTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTAtt                                                                                                                   <  2:679691/141‑1 (MQ=255)
                              tatataACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGc                                                                                                                >  1:649824/1‑141 (MQ=255)
                               atatcACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATGAGTTTGAACAGGTGCATACCTCGTAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCa                                                                                                               >  2:643655/1‑141 (MQ=255)
                                 ataACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCAtt                                                                                                             <  1:298781/141‑1 (MQ=255)
                                   aaCTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTta                                                                                                           >  2:306725/1‑141 (MQ=255)
                                   aaCTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTta                                                                                                           >  1:193220/1‑141 (MQ=255)
                                    aCTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTtaa                                                                                                          <  2:396140/141‑1 (MQ=255)
                                     cTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTtaat                                                                                                         <  1:484556/141‑1 (MQ=255)
                                      ttAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTtaata                                                                                                        >  2:888730/1‑141 (MQ=255)
                                          gtagtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACtt                                                                                                    >  2:455621/1‑141 (MQ=255)
                                          gtagtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACtt                                                                                                    >  2:689251/1‑141 (MQ=255)
                                            agtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTa                                                                                                  >  1:56048/1‑141 (MQ=255)
                                             gtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTaa                                                                                                 >  1:764589/1‑141 (MQ=255)
                                              taTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTaaa                                                                                                >  2:224417/1‑141 (MQ=255)
                                              taTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTaaa                                                                                                <  1:177748/141‑1 (MQ=255)
                                                tCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAata                                                                                              <  1:977683/141‑1 (MQ=255)
                                                 cAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAatat                                                                                             >  1:547604/1‑141 (MQ=255)
                                                  aaGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATAtt                                                                                            <  1:610697/141‑1 (MQ=255)
                                                   aGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTa                                                                                           <  2:500131/141‑1 (MQ=255)
                                                     aCGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAAt                                                                                         <  2:225596/141‑1 (MQ=255)
                                                     aCGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAAt                                                                                         >  2:622558/1‑141 (MQ=255)
                                                         aTCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGa                                                                                     >  2:552168/1‑141 (MQ=255)
                                                         aTCTATACTTGATGTGTAGTAATGTATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTAAATATTAATCTGa                                                                                     >  2:722399/1‑141 (MQ=255)
                                                          tCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGAt                                                                                    >  1:367079/1‑141 (MQ=255)
                                                            tataCTTGTTGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATAc                                                                                  >  2:893647/1‑141 (MQ=255)
                                                            tataCTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATAc                                                                                  >  1:577035/1‑141 (MQ=255)
                                                               aCTTGATGTGTAGTAATTTATTTACTATTAGTTTGAACATGTTCATAACTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTaa                                                                               <  1:148224/141‑1 (MQ=255)
                                                                  tGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATCTTAATCTGATACTAACat                                                                            >  2:431678/1‑141 (MQ=255)
                                                                  tGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACat                                                                            <  1:495560/141‑1 (MQ=255)
                                                                    aTGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACataa                                                                          <  2:625255/141‑1 (MQ=255)
                                                                    aTGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACataa                                                                          <  2:737692/141‑1 (MQ=255)
                                                                         taataATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCTTATTAGCATTTAATAACTTTAAATATTTATCTGATACTAACATAATAAAc                                                                     <  2:124046/138‑1 (MQ=255)
                                                                           gtaATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTg                                                                   <  1:8131/141‑1 (MQ=255)
                                                                           gtaATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTg                                                                   <  1:903821/141‑1 (MQ=255)
                                                                                 tATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTATCATTTAATAACCTTAAATATTAATCTGATACTAACATAATAAACTGTTTTaa                                                             <  1:837062/141‑1 (MQ=255)
                                                                                   tttACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATg                                                           >  2:837980/1‑141 (MQ=255)
                                                                                          ttAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACtt                                                    >  2:661351/1‑141 (MQ=255)
                                                                                          ttAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTAATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACtt                                                    >  1:646575/1‑141 (MQ=255)
                                                                                               ttGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATa                                               >  2:38149/1‑141 (MQ=255)
                                                                                                 gAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAgg                                             >  2:126488/1‑141 (MQ=255)
                                                                                                  aaCATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAggg                                            <  2:676676/141‑1 (MQ=255)
                                                                                                   aCATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGt                                           >  2:112606/1‑141 (MQ=255)
                                                                                                    cATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTa                                          <  1:306725/141‑1 (MQ=255)
                                                                                                     aTGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACttttaccctatgt                                         <  2:1034043/141‑12 (MQ=255)
                                                                                                      ttttCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATTTCTTAAAATGTTATTCTGAGACTAACATAATAAACTTTTTTAATGCGGTCTTACATAGGGTaaa                                        <  2:490949/139‑1 (MQ=255)
                                                                                                      tGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaa                                        >  1:634114/1‑141 (MQ=255)
                                                                                                      tGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaa                                        >  1:1060305/1‑141 (MQ=255)
                                                                                                       gTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaaa                                       >  2:106378/1‑141 (MQ=255)
                                                                                                         tCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAga                                     >  2:703850/1‑140 (MQ=255)
                                                                                                         tCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGt                                     <  2:675810/141‑1 (MQ=255)
                                                                                                           aTACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTcc                                   >  2:184552/1‑141 (MQ=255)
                                                                                                                tCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTac                              <  1:1049158/141‑1 (MQ=255)
                                                                                                                tCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTac                              <  2:751584/141‑1 (MQ=255)
                                                                                                                   taaataaaAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTAcaca                           <  2:747081/141‑1 (MQ=255)
                                                                                                                    aaataaaAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTACTCTGATACTAACATAATAAGCTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACAt                          <  1:888730/141‑1 (MQ=255)
                                                                                                                    aaataaaAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACAt                          <  2:764589/141‑1 (MQ=255)
                                                                                                                        aaaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAAcc                      >  1:246538/1‑141 (MQ=255)
                                                                                                                        aaaaaCACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAAcc                      <  1:519903/141‑1 (MQ=255)
                                                                                                                         aaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCa                     >  2:619864/1‑141 (MQ=255)
                                                                                                                         aaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCa                     >  1:500192/1‑141 (MQ=255)
                                                                                                                         aaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACAAAGGGTAAAAGTCCGCTACACATAACCa                     >  2:878191/1‑141 (MQ=255)
                                                                                                                          aaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCaa                    <  1:665378/141‑1 (MQ=255)
                                                                                                                            aGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAata                  <  2:424354/141‑1 (MQ=255)
                                                                                                                             gACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAatat                 >  2:1066758/1‑141 (MQ=255)
                                                                                                                              acacATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAAtata                <  1:18372/141‑1 (MQ=255)
                                                                                                                                acatcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAatactgg              >  2:404356/1‑137 (MQ=255)
                                                                                                                                 catcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACtt             <  1:1017705/141‑1 (MQ=255)
                                                                                                                                 catcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACtt             >  1:968201/1‑141 (MQ=255)
                                                                                                                                  atcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGATTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACttt            >  2:864270/1‑141 (MQ=255)
                                                                                                                                   tcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTg           <  2:136485/141‑1 (MQ=255)
                                                                                                                                   tcacatAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTg           >  1:406045/1‑141 (MQ=255)
                                                                                                                                    cacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGc          <  2:387104/141‑1 (MQ=255)
                                                                                                                                     acatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCt         <  1:144563/141‑1 (MQ=255)
                                                                                                                                       atAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTaa       >  1:727599/1‑141 (MQ=255)
                                                                                                                                        tAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAAc      <  2:56048/141‑1 (MQ=255)
                                                                                                                                         aGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACt     <  1:671672/141‑1 (MQ=255)
                                                                                                                                           tGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTa   >  1:89774/1‑141 (MQ=255)
                                                                                                                                            gATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTAt  >  1:25875/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTCGTCTTTAGTCATCATAATGTTTCACCTTATATAACTTAAGTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTAT  >  CP000730/2110737‑2111017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: