Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,120,625 A→G 8.5% D50D (GAT→GAC USA300HOU_1994 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,120,6250AG 8.5% 225.8 / 9.7 82D50D (GAT→GACUSA300HOU_1994hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base A (41/34);  new base G (4/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTT  >  CP000730/2120486‑2120765
                                                                                                                                           |                                                                                                                                             
gcggttctcctATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcat                                                                                                                                              <  1:125477/131‑1 (MQ=255)
 ttctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcatc                                                                                                                                             <  2:455805/141‑1 (MQ=255)
  tctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcatca                                                                                                                                            <  2:945759/141‑1 (MQ=255)
   ctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAg                                                                                                                                           >  2:189671/1‑141 (MQ=255)
     ttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTCCTCATCAGaa                                                                                                                                         <  2:423209/141‑1 (MQ=255)
       ttGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAAc                                                                                                                                       <  2:983169/140‑1 (MQ=255)
          tATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAg                                                                                                                                    >  2:436533/1‑141 (MQ=255)
              tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTACAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                                                                <  1:784846/141‑1 (MQ=255)
              tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                                                                <  1:989805/141‑1 (MQ=255)
              tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                                                                >  2:700825/1‑141 (MQ=255)
              tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                                                                <  1:61745/141‑1 (MQ=255)
               ctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAcc                                                                                                                               >  1:748148/1‑141 (MQ=255)
                 cATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCg                                                                                                                             >  2:160075/1‑141 (MQ=255)
                  aTGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGt                                                                                                                            >  2:909957/1‑141 (MQ=255)
                        ataCCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTg                                                                                                                      >  1:522359/1‑141 (MQ=255)
                         tacctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGa                                                                                                                     <  1:238647/141‑1 (MQ=255)
                         tacctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACCAGTAACCCGTCAATTGa                                                                                                                     >  1:603800/1‑141 (MQ=255)
                          acctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCNCATCAGAAACTANTAACCCGTCAATTGaa                                                                                                                    <  1:809794/141‑1 (MQ=255)
                            ctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAAtt                                                                                                                  <  1:449886/141‑1 (MQ=255)
                                 aTTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCtt                                                                                                             <  2:1030522/141‑1 (MQ=255)
                                  tttCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTc                                                                                                            <  2:890044/141‑1 (MQ=255)
                                    tCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCAt                                                                                                          <  2:385348/141‑1 (MQ=255)
                                     cATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATg                                                                                                         <  1:886707/141‑1 (MQ=255)
                                     cATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATg                                                                                                         >  2:723607/1‑141 (MQ=255)
                                      aTGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATgg                                                                                                        >  2:587370/1‑141 (MQ=255)
                                         aCTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCt                                                                                                     <  1:592804/141‑1 (MQ=255)
                                         aCTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCt                                                                                                     <  2:1076496/141‑1 (MQ=38)
                                             aGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaa                                                                                                 <  1:503076/141‑1 (MQ=255)
                                              gttaaTTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAaac                                                                                                >  2:1080817/5‑141 (MQ=38)
                                                tCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCGTAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaa                                                                                              <  1:852966/141‑1 (MQ=255)
                                                 cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaac                                                                                             >  1:714974/1‑141 (MQ=255)
                                                     aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt                                                                                         <  1:281820/141‑1 (MQ=255)
                                                      gTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAttt                                                                                        >  1:872486/1‑141 (MQ=255)
                                                           tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                   >  2:1065026/1‑141 (MQ=255)
                                                            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGAcac                                                                                  >  2:705909/1‑139 (MQ=255)
                                                            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                  >  1:663286/1‑141 (MQ=255)
                                                            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                  >  2:760113/1‑141 (MQ=255)
                                                                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                              >  1:644608/1‑141 (MQ=255)
                                                                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                              >  2:538008/1‑141 (MQ=255)
                                                                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                              >  2:923004/1‑141 (MQ=255)
                                                                 tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                             >  1:734959/1‑140 (MQ=255)
                                                                 tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                             >  1:526314/1‑140 (MQ=255)
                                                                 tCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATAc                                                                             <  2:1003342/141‑1 (MQ=255)
                                                                     cTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                         <  1:429292/141‑1 (MQ=255)
                                                                       gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                                                                       <  1:455632/141‑2 (MQ=255)
                                                                       gtgtTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGt                                                                       >  2:635553/1‑141 (MQ=255)
                                                                          ttATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTc                                                                    <  2:629719/141‑1 (MQ=255)
                                                                            aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                                                                  <  1:299598/141‑1 (MQ=255)
                                                                                 cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                             >  1:372840/1‑141 (MQ=255)
                                                                                 cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                             <  1:309741/141‑1 (MQ=255)
                                                                                  aaTCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAActct                                                            <  2:82870/141‑1 (MQ=255)
                                                                                   aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt                                                           <  1:846708/141‑1 (MQ=255)
                                                                                    tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                          >  1:475000/1‑141 (MQ=255)
                                                                                    tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                          >  1:992887/1‑141 (MQ=255)
                                                                                        ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg                                                      <  1:641314/141‑1 (MQ=255)
                                                                                            tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGaaaa                                                 >  2:1046637/1‑141 (MQ=255)
                                                                                              aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATc                                               <  2:730749/141‑1 (MQ=255)
                                                                                                cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaa                                             >  1:432525/1‑141 (MQ=255)
                                                                                                 acTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaaa                                            >  2:188678/1‑141 (MQ=255)
                                                                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                         >  1:1050983/1‑141 (MQ=255)
                                                                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                         >  2:101574/1‑141 (MQ=255)
                                                                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                         >  2:1054135/1‑141 (MQ=255)
                                                                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                         >  2:944847/1‑141 (MQ=255)
                                                                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                         >  1:940092/1‑141 (MQ=255)
                                                                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                         >  1:219361/1‑141 (MQ=255)
                                                                                                      gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCAt                                       <  1:929619/141‑1 (MQ=255)
                                                                                                            aaaTATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAAATCAAAGCCATCTACa                                  >  1:897473/1‑141 (MQ=255)
                                                                                                              atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                               <  2:669992/141‑1 (MQ=255)
                                                                                                              atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                               <  1:460266/141‑1 (MQ=255)
                                                                                                               tatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaa                              <  1:797050/141‑1 (MQ=255)
                                                                                                                 tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatt                            <  1:631767/141‑1 (MQ=255)
                                                                                                                 tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatt                            <  2:46529/141‑1 (MQ=255)
                                                                                                                    aaaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAattatt                         <  1:522513/141‑1 (MQ=255)
                                                                                                                     aaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttt                        >  2:1058932/1‑141 (MQ=255)
                                                                                                                      aGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAtttt                       <  1:576778/141‑1 (MQ=255)
                                                                                                                       gCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttttt                      >  1:712845/1‑141 (MQ=255)
                                                                                                                          gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGa                   >  2:409349/1‑141 (MQ=255)
                                                                                                                             tataGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACAt                <  1:439432/141‑1 (MQ=255)
                                                                                                                                     gTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc        <  1:617052/141‑1 (MQ=255)
                                                                                                                                      ttCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAccc       >  2:60905/1‑141 (MQ=255)
                                                                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg      >  2:883004/1‑141 (MQ=255)
                                                                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg      >  2:927714/1‑141 (MQ=255)
                                                                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg      >  2:625438/1‑141 (MQ=255)
                                                                                                                                        ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt     >  2:723508/1‑141 (MQ=255)
                                                                                                                                        ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt     >  2:723512/1‑141 (MQ=255)
                                                                                                                                           atcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGtttt  >  1:29322/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
TTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTT  >  CP000730/2120486‑2120765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: