Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,120,673 A→G 7.9% V34V (GTT→GTC USA300HOU_1994 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,120,6730AG 7.9% 248.2 / 10.3 89V34V (GTT→GTCUSA300HOU_1994hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base A (49/33);  new base G (5/2);  total (54/35)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

TCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATT  >  CP000730/2120534‑2120812
                                                                                                                                           |                                                                                                                                            
tCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCGTAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaa                                                                                                                                             <  1:852966/141‑1 (MQ=255)
 cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaac                                                                                                                                            >  1:714974/1‑141 (MQ=255)
     aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt                                                                                                                                        <  1:281820/141‑1 (MQ=255)
      gTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAttt                                                                                                                                       >  1:872486/1‑141 (MQ=255)
           tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                                                                  >  2:1065026/1‑141 (MQ=255)
            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGAcac                                                                                                                                 >  2:705909/1‑139 (MQ=255)
            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                 >  1:663286/1‑141 (MQ=255)
            ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                 >  2:760113/1‑141 (MQ=255)
                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                             >  1:644608/1‑141 (MQ=255)
                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                             >  2:538008/1‑141 (MQ=255)
                ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                             >  2:923004/1‑141 (MQ=255)
                 tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                            >  1:734959/1‑140 (MQ=255)
                 tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                            >  1:526314/1‑140 (MQ=255)
                 tCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATAc                                                                                                                            <  2:1003342/141‑1 (MQ=255)
                     cTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                                                                        <  1:429292/141‑1 (MQ=255)
                       gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                                                                                                                      <  1:455632/141‑2 (MQ=255)
                       gtgtTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGt                                                                                                                      >  2:635553/1‑141 (MQ=255)
                          ttATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTc                                                                                                                   <  2:629719/141‑1 (MQ=255)
                            aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                                                                                                                 <  1:299598/141‑1 (MQ=255)
                                 cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                            >  1:372840/1‑141 (MQ=255)
                                 cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                            <  1:309741/141‑1 (MQ=255)
                                  aaTCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAActct                                                                                                           <  2:82870/141‑1 (MQ=255)
                                   aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt                                                                                                          <  1:846708/141‑1 (MQ=255)
                                    tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                         >  1:475000/1‑141 (MQ=255)
                                    tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                         >  1:992887/1‑141 (MQ=255)
                                        ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg                                                                                                     <  1:641314/141‑1 (MQ=255)
                                            tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGaaaa                                                                                                >  2:1046637/1‑141 (MQ=255)
                                              aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATc                                                                                              <  2:730749/141‑1 (MQ=255)
                                                cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaa                                                                                            >  1:432525/1‑141 (MQ=255)
                                                 acTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaaa                                                                                           >  2:188678/1‑141 (MQ=255)
                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                        >  2:944847/1‑141 (MQ=255)
                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                        >  1:940092/1‑141 (MQ=255)
                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                        >  2:101574/1‑141 (MQ=255)
                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                        >  1:1050983/1‑141 (MQ=255)
                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                        >  1:219361/1‑141 (MQ=255)
                                                    ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                        >  2:1054135/1‑141 (MQ=255)
                                                      gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCAt                                                                                      <  1:929619/141‑1 (MQ=255)
                                                            aaaTATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAAATCAAAGCCATCTACa                                                                                 >  1:897473/1‑141 (MQ=255)
                                                              atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                                                                              <  1:460266/141‑1 (MQ=255)
                                                              atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                                                                              <  2:669992/141‑1 (MQ=255)
                                                               tatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaa                                                                             <  1:797050/141‑1 (MQ=255)
                                                                 tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatt                                                                           <  1:631767/141‑1 (MQ=255)
                                                                 tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatt                                                                           <  2:46529/141‑1 (MQ=255)
                                                                    aaaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAattatt                                                                        <  1:522513/141‑1 (MQ=255)
                                                                     aaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttt                                                                       >  2:1058932/1‑141 (MQ=255)
                                                                      aGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAtttt                                                                      <  1:576778/141‑1 (MQ=255)
                                                                       gCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttttt                                                                     >  1:712845/1‑141 (MQ=255)
                                                                          gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                                  >  2:409349/1‑141 (MQ=255)
                                                                             tataGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACAt                                                               <  1:439432/141‑1 (MQ=255)
                                                                                     gTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                       <  1:617052/141‑1 (MQ=255)
                                                                                      ttCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAccc                                                      >  2:60905/1‑141 (MQ=255)
                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                     >  2:927714/1‑141 (MQ=255)
                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                     >  2:625438/1‑141 (MQ=255)
                                                                                       tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                     >  2:883004/1‑141 (MQ=255)
                                                                                        ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                    >  2:723508/1‑141 (MQ=255)
                                                                                        ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                    >  2:723512/1‑141 (MQ=255)
                                                                                         tcgtcaGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGtt                                                   >  1:22892/4‑141 (MQ=255)
                                                                                           atcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGtttt                                                 >  1:29322/1‑141 (MQ=255)
                                                                                             caGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGt                                               <  1:34641/141‑1 (MQ=255)
                                                                                              aGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCTTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTa                                              >  1:340122/1‑141 (MQ=255)
                                                                                               gAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTaa                                             <  2:379579/141‑1 (MQ=255)
                                                                                                    tagtagCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACAt                                        >  1:715115/1‑141 (MQ=255)
                                                                                                    tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                       <  1:1074934/126‑1 (MQ=255)
                                                                                                    tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                       >  2:1074934/1‑126 (MQ=255)
                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       >  2:993461/1‑141 (MQ=255)
                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       >  1:1014721/1‑141 (MQ=255)
                                                                                                     aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                       <  1:923004/141‑1 (MQ=255)
                                                                                                      gTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTg                                      >  2:176986/1‑141 (MQ=255)
                                                                                                        aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                                    >  1:26639/1‑141 (MQ=255)
                                                                                                             gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                               >  1:789354/1‑141 (MQ=255)
                                                                                                             gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                               >  1:572862/1‑141 (MQ=255)
                                                                                                               cAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcc                             >  2:400888/1‑141 (MQ=255)
                                                                                                                aaTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcct                            >  2:780143/1‑141 (MQ=255)
                                                                                                                 aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATAAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                           <  1:590673/141‑1 (MQ=255)
                                                                                                                  ttGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctcc                          <  1:635701/141‑1 (MQ=255)
                                                                                                                   tGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTcctcct                         <  2:205492/141‑1 (MQ=255)
                                                                                                                    gAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCtt                        >  2:114196/1‑141 (MQ=255)
                                                                                                                     aaTTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTg                       >  1:1084372/1‑141 (MQ=255)
                                                                                                                       tttCCTCCCTAGTCTAGAACAACCATTTCGACGCAATACGCTCGCTTCTTAACTCTTAATTGAAAAGCAAAGCCATGTACCTTAGTTCTTTTTCGCCATTCGTCACCCGTTTCGTAACGCATTGTTCTCGTCCTCCTTGTc                     <  2:777534/141‑1 (MQ=255)
                                                                                                                        ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGGCATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa                    <  1:842338/141‑1 (MQ=255)
                                                                                                                         tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCCTCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTATTACATTGTTCTCGTCCTCCTTGTCAt                   >  1:217886/1‑141 (MQ=255)
                                                                                                                         tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAt                   >  1:962909/1‑141 (MQ=255)
                                                                                                                          ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTGATACATTGTTCTCGTCCTCCTTGTCAtt                  >  1:755978/1‑141 (MQ=255)
                                                                                                                          ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAtt                  >  1:996421/1‑141 (MQ=255)
                                                                                                                              cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAACATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTAGTGTAGTACAGTGTTCCTGACGTCTTTGTCATTATCt              >  2:271327/1‑141 (MQ=255)
                                                                                                                              cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt              <  1:766597/141‑1 (MQ=255)
                                                                                                                               aTGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGCAAATCAAAGCCATCTACATTAATTATTTTTCGACATACTTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCta             <  2:754503/141‑1 (MQ=255)
                                                                                                                                tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat            >  1:861688/1‑141 (MQ=255)
                                                                                                                                      aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATNCATNGTTCTCGTCCTCCTTNTCATTATCTATAGCTAg      <  1:927714/141‑1 (MQ=255)
                                                                                                                                      aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg      <  1:249101/141‑1 (MQ=255)
                                                                                                                                      aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg      <  1:700825/141‑1 (MQ=255)
                                                                                                                                          caacCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt  >  1:21411/1‑141 (MQ=255)
                                                                                                                                          caacCATNTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt  <  2:816252/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATT  >  CP000730/2120534‑2120812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: