Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,120,676 C→T 7.7% M33I (ATG→ATA)  USA300HOU_1994 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,120,6760CT 7.7% 275.4 / 8.5 91M33I (ATG→ATA) USA300HOU_1994hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base C (50/34);  new base T (5/2);  total (55/36)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

AGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTT  >  CP000730/2120539‑2120815
                                                                                                                                         |                                                                                                                                            
aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt                                                                                                                                           <  1:281820/141‑1 (MQ=255)
 gTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAttt                                                                                                                                          >  1:872486/1‑141 (MQ=255)
      tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                                                                     >  2:1065026/1‑141 (MQ=255)
       ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGAcac                                                                                                                                    >  2:705909/1‑139 (MQ=255)
       ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                    >  1:663286/1‑141 (MQ=255)
       ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                    >  2:760113/1‑141 (MQ=255)
           ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                                >  2:923004/1‑141 (MQ=255)
           ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                                >  1:644608/1‑141 (MQ=255)
           ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                                >  2:538008/1‑141 (MQ=255)
            tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                               >  1:734959/1‑140 (MQ=255)
            tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                               >  1:526314/1‑140 (MQ=255)
            tCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATAc                                                                                                                               <  2:1003342/141‑1 (MQ=255)
                cTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                                                                           <  1:429292/141‑1 (MQ=255)
                  gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                                                                                                                         <  1:455632/141‑2 (MQ=255)
                  gtgtTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGt                                                                                                                         >  2:635553/1‑141 (MQ=255)
                     ttATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTc                                                                                                                      <  2:629719/141‑1 (MQ=255)
                       aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                                                                                                                    <  1:299598/141‑1 (MQ=255)
                            cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                               >  1:372840/1‑141 (MQ=255)
                            cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                               <  1:309741/141‑1 (MQ=255)
                             aaTCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAActct                                                                                                              <  2:82870/141‑1 (MQ=255)
                              aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt                                                                                                             <  1:846708/141‑1 (MQ=255)
                               tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                            >  1:992887/1‑141 (MQ=255)
                               tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                            >  1:475000/1‑141 (MQ=255)
                                   ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg                                                                                                        <  1:641314/141‑1 (MQ=255)
                                       tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑aaaa                                                                                                   >  2:1046637/1‑141 (MQ=255)
                                         aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATc                                                                                                 <  2:730749/141‑1 (MQ=255)
                                           cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCaa                                                                                               >  1:432525/1‑141 (MQ=255)
                                            acTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCaaa                                                                                              >  2:188678/1‑141 (MQ=255)
                                               ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                           >  1:219361/1‑141 (MQ=255)
                                               ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                           >  2:1054135/1‑141 (MQ=255)
                                               ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                           >  1:1050983/1‑141 (MQ=255)
                                               ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                           >  1:940092/1‑141 (MQ=255)
                                               ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                           >  2:944847/1‑141 (MQ=255)
                                               ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGcc                                                                                           >  2:101574/1‑141 (MQ=255)
                                                 gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCAt                                                                                         <  1:929619/141‑1 (MQ=255)
                                                       aaaTATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAAATCAAAGCCATCTACa                                                                                    >  1:897473/1‑141 (MQ=255)
                                                         atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACatta                                                                                 <  1:460266/141‑1 (MQ=255)
                                                         atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACatta                                                                                 <  2:669992/141‑1 (MQ=255)
                                                          tatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACattaa                                                                                <  1:797050/141‑1 (MQ=255)
                                                            tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACattaatt                                                                              <  2:46529/141‑1 (MQ=255)
                                                            tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACattaatt                                                                              <  1:631767/141‑1 (MQ=255)
                                                               aaaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAattatt                                                                           <  1:522513/141‑1 (MQ=255)
                                                                aaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTAttt                                                                          >  2:1058932/1‑141 (MQ=255)
                                                                 aGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTAtttt                                                                         <  1:576778/141‑1 (MQ=255)
                                                                  gCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTAttttt                                                                        >  1:712845/1‑141 (MQ=255)
                                                                     gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                                     >  2:409349/1‑141 (MQ=255)
                                                                        tataGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACAt                                                                  <  1:439432/141‑1 (MQ=255)
                                                                                gTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                          <  1:617052/141‑1 (MQ=255)
                                                                                 ttCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAccc                                                         >  2:60905/1‑141 (MQ=255)
                                                                                  tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                        >  2:625438/1‑141 (MQ=255)
                                                                                  tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                        >  2:883004/1‑141 (MQ=255)
                                                                                  tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                        >  2:927714/1‑141 (MQ=255)
                                                                                   ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                       >  2:723512/1‑141 (MQ=255)
                                                                                   ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                       >  2:723508/1‑141 (MQ=255)
                                                                                    tcgtcaGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGtt                                                      >  1:22892/4‑141 (MQ=255)
                                                                                      atcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGtttt                                                    >  1:29322/1‑141 (MQ=255)
                                                                                        caGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGt                                                  <  1:34641/141‑1 (MQ=255)
                                                                                         aGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCTTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTa                                                 >  1:340122/1‑141 (MQ=255)
                                                                                          gAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTaa                                                <  2:379579/141‑1 (MQ=255)
                                                                                               tagtagCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACAt                                           >  1:715115/1‑141 (MQ=255)
                                                                                               tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                          <  1:1074934/126‑1 (MQ=255)
                                                                                               tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                          >  2:1074934/1‑126 (MQ=255)
                                                                                                aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                          >  1:1014721/1‑141 (MQ=255)
                                                                                                aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                          >  2:993461/1‑141 (MQ=255)
                                                                                                aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                          <  1:923004/141‑1 (MQ=255)
                                                                                                 gTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTg                                         >  2:176986/1‑141 (MQ=255)
                                                                                                   aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                                       >  1:26639/1‑141 (MQ=255)
                                                                                                        gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                                  >  1:572862/1‑141 (MQ=255)
                                                                                                        gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                                  >  1:789354/1‑141 (MQ=255)
                                                                                                          cAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcc                                >  2:400888/1‑141 (MQ=255)
                                                                                                           aaTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcct                               >  2:780143/1‑141 (MQ=255)
                                                                                                            aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATAAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                              <  1:590673/141‑1 (MQ=255)
                                                                                                             ttGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctcc                             <  1:635701/141‑1 (MQ=255)
                                                                                                              tGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTcctcct                            <  2:205492/141‑1 (MQ=255)
                                                                                                               gAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCtt                           >  2:114196/1‑141 (MQ=255)
                                                                                                                aaTTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTg                          >  1:1084372/1‑141 (MQ=255)
                                                                                                                  tttCCTCCCTAGTCTAGAACAACCATTTCGACGCAATACGCTCGCTTCTTAACTCTTAATTG‑AAAAGCAAAGCCATGTACCTTAGTTCTTTTTCGCCATTCGTCACCCGTTTCGTAACGCATTGTTCTCGTCCTCCTTGTc                        <  2:777534/141‑1 (MQ=255)
                                                                                                                   ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGGCATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa                       <  1:842338/141‑1 (MQ=255)
                                                                                                                    tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCCTCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTATTACATTGTTCTCGTCCTCCTTGTCAt                      >  1:217886/1‑141 (MQ=255)
                                                                                                                    tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAt                      >  1:962909/1‑141 (MQ=255)
                                                                                                                     ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTGATACATTGTTCTCGTCCTCCTTGTCAtt                     >  1:755978/1‑141 (MQ=255)
                                                                                                                     ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAtt                     >  1:996421/1‑141 (MQ=255)
                                                                                                                         cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AACATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTAGTGTAGTACAGTGTTCCTGACGTCTTTGTCATTATCt                 >  2:271327/1‑141 (MQ=255)
                                                                                                                         cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt                 <  1:766597/141‑1 (MQ=255)
                                                                                                                          aTGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑CAAATCAAAGCCATCTACATTAATTATTTTTCGACATACTTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCta                <  2:754503/141‑1 (MQ=255)
                                                                                                                           tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat               >  1:861688/1‑141 (MQ=255)
                                                                                                                                 aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATNCATNGTTCTCGTCCTCCTTNTCATTATCTATAGCTAg         <  1:927714/141‑1 (MQ=255)
                                                                                                                                 aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg         <  1:700825/141‑1 (MQ=255)
                                                                                                                                 aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg         <  1:249101/141‑1 (MQ=255)
                                                                                                                                     caacCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt     >  1:21411/1‑141 (MQ=255)
                                                                                                                                     caacCATNTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt     <  2:816252/141‑1 (MQ=255)
                                                                                                                                        gcATTTCGACGCTGTACGCTCGTTTCTTAACTCTTAATGG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAttttt  <  2:638307/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
AGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTG‑AAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTT  >  CP000730/2120539‑2120815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑