Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,120,685 G→A 8.1% S30S (AGC→AGT USA300HOU_1994 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,120,6850GA 8.1% 293.6 / 11.2 98S30S (AGC→AGTUSA300HOU_1994hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base G (53/37);  new base A (5/3);  total (58/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 102 total aligned reads displayed.

TCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCATTTT  >  CP000730/2120545‑2120825
                                                                                                                                            |                                                                                                                                             
tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                                                                                                                               >  2:1065026/1‑141 (MQ=255)
 ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                              >  1:663286/1‑141 (MQ=255)
 ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                                                                                                                              >  2:760113/1‑141 (MQ=255)
     ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                                          >  2:923004/1‑141 (MQ=255)
     ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                                          >  1:644608/1‑141 (MQ=255)
     ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                                                                                                                          >  2:538008/1‑141 (MQ=255)
      tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                                         >  1:526314/1‑140 (MQ=255)
      tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                                                                                                                         >  1:734959/1‑140 (MQ=255)
      tCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATAc                                                                                                                                         <  2:1003342/141‑1 (MQ=255)
          cTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                                                                                                                                     <  1:429292/141‑1 (MQ=255)
            gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                                                                                                                                   <  1:455632/141‑2 (MQ=255)
            gtgtTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGt                                                                                                                                   >  2:635553/1‑141 (MQ=255)
               ttATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTc                                                                                                                                <  2:629719/141‑1 (MQ=255)
                 aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                                                                                                                              <  1:299598/141‑1 (MQ=255)
                      cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                                         >  1:372840/1‑141 (MQ=255)
                      cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                                                                                                                         <  1:309741/141‑1 (MQ=255)
                       aaTCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAActct                                                                                                                        <  2:82870/141‑1 (MQ=255)
                        aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt                                                                                                                       <  1:846708/141‑1 (MQ=255)
                         tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                                      >  1:992887/1‑141 (MQ=255)
                         tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa                                                                                                                      >  1:475000/1‑141 (MQ=255)
                             ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg                                                                                                                  <  1:641314/141‑1 (MQ=255)
                                 tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGaaaa                                                                                                             >  2:1046637/1‑141 (MQ=255)
                                   aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATc                                                                                                           <  2:730749/141‑1 (MQ=255)
                                     cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaa                                                                                                         >  1:432525/1‑141 (MQ=255)
                                      acTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaaa                                                                                                        >  2:188678/1‑141 (MQ=255)
                                         ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                                     >  2:101574/1‑141 (MQ=255)
                                         ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                                     >  2:1054135/1‑141 (MQ=255)
                                         ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                                     >  1:1050983/1‑141 (MQ=255)
                                         ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                                     >  2:944847/1‑141 (MQ=255)
                                         ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                                     >  1:219361/1‑141 (MQ=255)
                                         ttGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGcc                                                                                                     >  1:940092/1‑141 (MQ=255)
                                           gTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCAt                                                                                                   <  1:929619/141‑1 (MQ=255)
                                                 aaaTATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAAATCAAAGCCATCTACa                                                                                              >  1:897473/1‑141 (MQ=255)
                                                   atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                                                                                           <  2:669992/141‑1 (MQ=255)
                                                   atatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACatta                                                                                           <  1:460266/141‑1 (MQ=255)
                                                    tatCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaa                                                                                          <  1:797050/141‑1 (MQ=255)
                                                      tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatt                                                                                        <  2:46529/141‑1 (MQ=255)
                                                      tCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACattaatt                                                                                        <  1:631767/141‑1 (MQ=255)
                                                         aaaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAattatt                                                                                     <  1:522513/141‑1 (MQ=255)
                                                          aaGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttt                                                                                    >  2:1058932/1‑141 (MQ=255)
                                                           aGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAtttt                                                                                   <  1:576778/141‑1 (MQ=255)
                                                            gCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTAttttt                                                                                  >  1:712845/1‑141 (MQ=255)
                                                               gtgtATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGa                                                                               >  2:409349/1‑141 (MQ=255)
                                                                  tataGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACAt                                                                            <  1:439432/141‑1 (MQ=255)
                                                                          gTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                                    <  1:617052/141‑1 (MQ=255)
                                                                           ttCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAccc                                                                   >  2:60905/1‑141 (MQ=255)
                                                                            tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                                  >  2:625438/1‑141 (MQ=255)
                                                                            tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                                  >  2:883004/1‑141 (MQ=255)
                                                                            tctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCg                                                                  >  2:927714/1‑141 (MQ=255)
                                                                             ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                                 >  2:723508/1‑141 (MQ=255)
                                                                             ctcATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGt                                                                 >  2:723512/1‑141 (MQ=255)
                                                                              tcgtcaGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGtt                                                                >  1:22892/4‑141 (MQ=255)
                                                                                atcaGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGtttt                                                              >  1:29322/1‑141 (MQ=255)
                                                                                  caGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGt                                                            <  1:34641/141‑1 (MQ=255)
                                                                                   aGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCTTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTa                                                           >  1:340122/1‑141 (MQ=255)
                                                                                    gAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTaa                                                          <  2:379579/141‑1 (MQ=255)
                                                                                         tagtagCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACAt                                                     >  1:715115/1‑141 (MQ=255)
                                                                                         tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                                    <  1:1074934/126‑1 (MQ=255)
                                                                                         tAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCAcc                                                                    >  2:1074934/1‑126 (MQ=255)
                                                                                          aGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACAtt                                                    >  1:1014721/1‑141 (MQ=255)
                                                                                           gTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTg                                                   >  2:176986/1‑141 (MQ=255)
                                                                                             aaCCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGtt                                                 >  1:26639/1‑141 (MQ=255)
                                                                                                  gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                                            >  1:789354/1‑141 (MQ=255)
                                                                                                  gTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGt                                            >  1:572862/1‑141 (MQ=255)
                                                                                                    cAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcc                                          >  2:400888/1‑141 (MQ=255)
                                                                                                     aaTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcct                                         >  2:780143/1‑141 (MQ=255)
                                                                                                      aTTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATAAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctc                                        <  1:590673/141‑1 (MQ=255)
                                                                                                       ttGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGtcctcc                                       <  1:635701/141‑1 (MQ=255)
                                                                                                        tGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTcctcct                                      <  2:205492/141‑1 (MQ=255)
                                                                                                         gAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCtt                                     >  2:114196/1‑141 (MQ=255)
                                                                                                          aaTTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTg                                    >  1:1084372/1‑141 (MQ=255)
                                                                                                            tttCCTCCCTAGTCTAGAACAACCATTTCGACGCAATACGCTCGCTTCTTAACTCTTAATTGAAAAGCAAAGCCATGTACCTTAGTTCTTTTTCGCCATTCGTCACCCGTTTCGTAACGCATTGTTCTCGTCCTCCTTGTc                                  <  2:777534/141‑1 (MQ=255)
                                                                                                             ttCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGGCATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCa                                 <  1:842338/141‑1 (MQ=255)
                                                                                                              tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCCTCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTATTACATTGTTCTCGTCCTCCTTGTCAt                                >  1:217886/1‑141 (MQ=255)
                                                                                                              tCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAt                                >  1:962909/1‑141 (MQ=255)
                                                                                                               ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTGATACATTGTTCTCGTCCTCCTTGTCAtt                               >  1:755978/1‑141 (MQ=255)
                                                                                                               ccTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCAtt                               >  1:996421/1‑141 (MQ=255)
                                                                                                                   cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAACATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTAGTGTAGTACAGTGTTCCTGACGTCTTTGTCATTATCt                           >  2:271327/1‑141 (MQ=255)
                                                                                                                   cATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCt                           <  1:766597/141‑1 (MQ=255)
                                                                                                                    aTGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGCAAATCAAAGCCATCTACATTAATTATTTTTCGACATACTTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCta                          <  2:754503/141‑1 (MQ=255)
                                                                                                                     tGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCtat                         >  1:861688/1‑141 (MQ=255)
                                                                                                                           aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATNCATNGTTCTCGTCCTCCTTNTCATTATCTATAGCTAg                   <  1:927714/141‑1 (MQ=255)
                                                                                                                           aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg                   <  1:249101/141‑1 (MQ=255)
                                                                                                                           aGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAg                   <  1:700825/141‑1 (MQ=255)
                                                                                                                               cgactaTTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAAtt               <  1:705909/136‑1 (MQ=255)
                                                                                                                               caacCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt               >  1:21411/1‑141 (MQ=255)
                                                                                                                               caacCATNTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAtt               <  2:816252/141‑1 (MQ=255)
                                                                                                                                 actaTTTCGACACTATATGCTCGCTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAAATATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAAtttt             >  2:153837/4‑141 (MQ=255)
                                                                                                                                  gcATTTCGACGCTGTACGCTCGTTTCTTAACTCTTAATGGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAAttttt            <  2:638307/140‑1 (MQ=255)
                                                                                                                                     tttCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGTCTTACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAAc         >  2:38862/1‑141 (MQ=255)
                                                                                                                                     tttCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAAc         >  1:66218/1‑141 (MQ=255)
                                                                                                                                      ttCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACg        <  1:189671/141‑1 (MQ=255)
                                                                                                                                       tCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGc       >  2:540546/1‑141 (MQ=255)
                                                                                                                                        cGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCa      >  2:600363/1‑141 (MQ=255)
                                                                                                                                        cGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCa      >  2:366528/1‑141 (MQ=255)
                                                                                                                                         gACGCTATACGCTCGTTTCTTAACTCTTAATTGATAATCAAAGCCATCTACATTAATTATTTTTCGTCATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCAt     <  2:268482/141‑1 (MQ=255)
                                                                                                                                         gACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCAt     <  1:671547/141‑1 (MQ=255)
                                                                                                                                            gCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTTTTCATACTTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCAtttt  >  2:316756/1‑141 (MQ=255)
                                                                                                                                            gCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCAtttt  <  1:436533/141‑1 (MQ=255)
                                                                                                                                            gCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCAtttt  >  1:949581/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAAAGCCATCTACATTAATTATTTTTCGACATACGTCACCCGTTTTGTAATACATTGTTCTCGTCCTCCTTGTCATTATCTATAGCTAGAATTTTTAACGCATTTT  >  CP000730/2120545‑2120825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: