Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,189,877	G→T	7.2%	R100R (CGA→AGA)	alr2 ←	alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,189,877	0	G	T	7.2%	294.6 / 8.1	92	R100R (CGA→AGA)	alr2	alanine racemase
Reads supporting (aligned to +/- strand):  ref base G (44/41);  new base T (2/5);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

CGTTTTAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTAAAT  >  CP000730/2189737‑2190017                                                                                                                                             |                                                                                                                                             cGTTTTAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCg                                                                                                                                              >  1:671289/1‑141 (MQ=255)   ttttAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTTTTTTTTTTTCTAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACAGTTAAGGCAACTCGgt                                                                                                                                            >  2:823929/1‑141 (MQ=255)     ttAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGgtgt                                                                                                                                          <  1:396868/141‑1 (MQ=255)     ttAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGgtgt                                                                                                                                          <  1:100145/141‑1 (MQ=255)       aGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGtgttg                                                                                                                                        >  1:821494/1‑141 (MQ=255)       aGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGtgttg                                                                                                                                        >  2:607231/1‑141 (MQ=255)       aGTATCTTTAATACCTAAACGTCCCATTACTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGtgttg                                                                                                                                        <  1:446819/141‑1 (MQ=255)         tATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGCCGGAACCGTTAAGGCAACTCTGTGTTGTa                                                                                                                                      <  2:313159/141‑1 (MQ=255)          aTCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTAt                                                                                                                                     <  2:159292/141‑1 (MQ=255)             tttAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGc                                                                                                                                  <  2:468312/141‑1 (MQ=255)             tttAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGc                                                                                                                                  <  1:769831/141‑1 (MQ=255)              ttAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCt                                                                                                                                 <  1:758923/141‑1 (MQ=255)                 aTACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTa                                                                                                                              >  1:611161/1‑141 (MQ=255)                 aTACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTa                                                                                                                              >  1:746249/1‑141 (MQ=255)                   aCCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATc                                                                                                                            <  2:287187/141‑1 (MQ=255)                     cTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCaa                                                                                                                          <  2:183592/141‑1 (MQ=255)                      tAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAat                                                                                                                         >  1:771350/1‑141 (MQ=255)                       aaaCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAata                                                                                                                        <  2:756173/141‑1 (MQ=255)                        aaCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAatat                                                                                                                       <  2:395074/141‑1 (MQ=255)                        aaCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAatat                                                                                                                       >  2:699224/1‑141 (MQ=255)                         aCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAGCCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATc                                                                                                                      <  2:1007409/141‑1 (MQ=255)                         aCGTCCCATGCCTGTAGCTAATGTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTATACGGAACCGTTAAGGCGACTCTGTGTTGTATCGCTTTATCAATATc                                                                                                                      <  2:99019/141‑1 (MQ=255)                            tCCCATTCCTGTTTCTAATTTAATGTGCATCCATAACCTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCTACCACTTTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  1:955494/141‑1 (MQ=255)                            tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAGATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  2:994452/141‑1 (MQ=255)                            tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  1:674888/141‑1 (MQ=255)                            tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   >  1:533294/1‑141 (MQ=255)                              ccATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAg                                                                                                                 >  2:340739/1‑141 (MQ=255)                              ccATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAg                                                                                                                 >  2:340681/1‑141 (MQ=255)                               cATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGc                                                                                                                <  2:410703/141‑1 (MQ=255)                                    cTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGCTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTATCTGGTa                                                                                                           >  1:550055/1‑141 (MQ=255)                                     tGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTaa                                                                                                          <  1:502675/141‑1 (MQ=255)                                       tATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAaca                                                                                                        <  1:12596/141‑1 (MQ=255)                                         tCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAacaca                                                                                                      >  1:630352/1‑141 (MQ=255)                                          cTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAacacac                                                                                                     >  1:349221/1‑141 (MQ=255)                                             aTTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTa                                                                                                  >  1:595682/1‑141 (MQ=255)                                             aTTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTa                                                                                                  >  1:334808/1‑141 (MQ=255)                                                  atttgCAACCATAACTTTTGCTCTTGCTCACCAGAGATGTTTTTAAGTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACGCGGTGTTGTATCGCTTTATGAATATCTTTAGGTGGTAACACACCTAAGACt                                                                                             <  2:867070/138‑1 (MQ=255)                                                  aTGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCTCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACt                                                                                             <  1:572167/141‑1 (MQ=255)                                                  aTGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACt                                                                                             <  2:30441/141‑1 (MQ=255)                                                    gtgCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTaa                                                                                           >  2:883855/1‑141 (MQ=255)                                                     tgCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTaaa                                                                                          >  1:767349/1‑141 (MQ=255)                                                           cATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTa                                                                                    <  2:577744/141‑1 (MQ=255)                                                             tcaCTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGc                                                                                  <  1:661289/139‑1 (MQ=255)                                                             tAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGc                                                                                  <  1:604170/141‑1 (MQ=255)                                                               aCTTTTTCTCTTGCTCACCAGAAATGTTTTTTTTTTTTTTTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTATCTGGTAACACACCTAATACTAAAATTTTAGCAg                                                                                >  2:745612/1‑141 (MQ=255)                                                                    tttttttttttttcAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCACTTTATTAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATc                                                                           >  2:369507/14‑141 (MQ=255)                                                                    ttCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATc                                                                           <  1:472743/141‑1 (MQ=255)                                                                     tctcTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATcc                                                                          >  2:475354/1‑141 (MQ=255)                                                                     tctcTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATcc                                                                          >  1:559450/1‑141 (MQ=255)                                                                       tctTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCa                                                                        <  1:425499/141‑1 (MQ=255)                                                                       tctTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCa                                                                        >  2:528356/1‑141 (MQ=255)                                                                          tGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGc                                                                     >  1:1055452/1‑141 (MQ=255)                                                                          tGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGc                                                                     >  1:260132/1‑141 (MQ=255)                                                                           gCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCa                                                                    <  2:374854/141‑1 (MQ=255)                                                                           gCTCACCAGAAATGTATCGACGTTCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCa                                                                    <  1:9762/141‑1 (MQ=255)                                                                           ccTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGGCGGAACCGCTAAGGCACCTCGGTGTTGTATCCCTTTATCAATATCCTTCGCTGGTAACACACCTAAGGCGACAATTTTAGCATTAATCCCATGCa                                                                    <  2:759171/140‑1 (MQ=255)                                                                               aCCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCt                                                                >  2:785458/1‑141 (MQ=255)                                                                                ccAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTa                                                               <  1:487909/141‑1 (MQ=255)                                                                                  aGAAATGTTTTTTTTTTTTTTTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAg                                                             >  2:443264/1‑141 (MQ=255)                                                                                  aGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAg                                                             >  2:828717/1‑141 (MQ=255)                                                                                    aaaTGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGtt                                                           >  2:158953/1‑141 (MQ=255)                                                                                      aTGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCt                                                         >  1:687823/1‑141 (MQ=255)                                                                                        gTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTAt                                                       >  1:644934/1‑141 (MQ=255)                                                                                        gTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTAt                                                       <  1:128063/141‑1 (MQ=255)                                                                                           tttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGc                                                    <  1:155502/141‑1 (MQ=255)                                                                                           tttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGc                                                    <  1:961898/141‑1 (MQ=255)                                                                                            ttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATCTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCt                                                   <  2:255673/141‑1 (MQ=255)                                                                                                  gCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCt                                             >  1:625694/1‑141 (MQ=255)                                                                                                  gCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCt                                             >  1:552983/1‑141 (MQ=255)                                                                                                  gCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGAAACACCCCTAAGAATAACATTTTAGCAGTAATCCTATGAATTCTAAGTTCTATCGCTTCATCt                                             >  1:914936/1‑141 (MQ=255)                                                                                                   cttcttTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTa                                            >  2:124169/1‑141 (MQ=255)                                                                                                   cttcttTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTa                                            >  1:303774/1‑141 (MQ=255)                                                                                                            aCCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTa                                   >  1:70230/1‑141 (MQ=255)                                                                                                             ccACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTAc                                  <  1:527861/141‑1 (MQ=255)                                                                                                             ccACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTAc                                  >  1:578415/1‑141 (MQ=255)                                                                                                              cACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACa                                 <  1:814613/141‑1 (MQ=255)                                                                                                                cTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACGACAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGc                               >  2:844771/1‑141 (MQ=255)                                                                                                                 tGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCa                              >  1:678771/1‑141 (MQ=255)                                                                                                                       gACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAAt                        >  1:1038159/1‑141 (MQ=255)                                                                                                                       gACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATAGCTTCATCTAACGTTGCTACAGCAAAAAAt                        >  1:48114/1‑141 (MQ=255)                                                                                                                         cGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAAtgt                      >  1:365570/1‑141 (MQ=255)                                                                                                                             ttcGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCTCTTCATCTAACGTTGCTACAGCAAAAAATGTGgcg                  <  2:58233/139‑1 (MQ=255)                                                                                                                             aCCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGgcg                  <  1:943704/141‑1 (MQ=255)                                                                                                                             aCCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGgcg                  <  1:475971/141‑1 (MQ=255)                                                                                                                               cGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGcc                >  2:1047141/1‑141 (MQ=255)                                                                                                                                 ttAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCAt              >  2:443666/1‑141 (MQ=255)                                                                                                                                   ggggCAACTCGGTGTTGTATCGCTTTATGGATGTCTGTTGCTGGTAGCACACCTAAGACTAGAATTTTAGCAGTAAGCACATCCATTCTAAGGTCTGTCGCTTCATCTACCGTGGCGACAGCAAAAAATGTGGCGCCAttt            <  2:707128/139‑1 (MQ=255)                                                                                                                                   aaGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCAttt            <  2:1022326/141‑1 (MQ=255)                                                                                                                                   aaGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCAttt            <  2:685673/141‑1 (MQ=255)                                                                                                                                      gCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTcc         <  1:524448/141‑1 (MQ=255)                                                                                                                                          cTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTa     <  2:533294/141‑1 (MQ=255)                                                                                                                                          cTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTa     >  1:931449/1‑141 (MQ=255)                                                                                                                                          cTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTa     >  1:191250/1‑141 (MQ=255)                                                                                                                                             ggTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTAAAt  >  2:367629/1‑141 (MQ=255)                                                                                                                                             ggTGATGTATCGCATTATCAGTATCTTTAGCTGGTAACACACCTAAGACTAAAATGTTAGCAGTTATCCCATGCATGCTATGTTCGATCGCTTCCTCTAACGATGCTACAGCAAAAAACGTGTCGCCATTTTCCATTAAAt  <  2:996658/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             CGTTTTAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTAAAT  >  CP000730/2189737‑2190017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑