Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,330,698 T→A 5.2% G95G (GGA→GGT lacD ← tagatose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,330,6980TA 5.2% 376.8 / 2.3 125G95G (GGA→GGTlacDtagatose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (50/65);  new base A (2/6);  total (53/72)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

TTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACTTTTAATTGTTCAA  >  CP000730/2330558‑2330837
                                                                                                                                            |                                                                                                                                           
ttttGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCatat                                                                                                                                             <  1:398908/141‑1 (MQ=255)
  ttGAATGTTAATTTCTTCAGCATCATCGACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTCAAACGTCGTGCAGACCACTCTACGCAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATAGcc                                                                                                                                           <  2:681165/141‑1 (MQ=255)
  ttGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATcc                                                                                                                                           <  1:711652/141‑1 (MQ=255)
  ttGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATcc                                                                                                                                           <  2:378466/141‑1 (MQ=255)
     aaTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGt                                                                                                                                        <  2:1021987/141‑1 (MQ=255)
     aaTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGt                                                                                                                                        >  1:883204/1‑141 (MQ=255)
     aaTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGt                                                                                                                                        >  2:487733/1‑141 (MQ=255)
     aaTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGt                                                                                                                                        >  1:728452/1‑141 (MQ=255)
     aaTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGt                                                                                                                                        >  2:662394/1‑141 (MQ=255)
     aaTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCACCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCATTTACGTCATAACCAGt                                                                                                                                        >  1:497565/1‑141 (MQ=255)
      aTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtt                                                                                                                                       <  1:549999/141‑1 (MQ=255)
      aTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtt                                                                                                                                       <  1:549642/141‑1 (MQ=255)
      aTGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtt                                                                                                                                       <  1:150669/141‑1 (MQ=255)
        gTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtttt                                                                                                                                     <  1:552995/141‑1 (MQ=255)
        gTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtttt                                                                                                                                     <  2:301846/141‑1 (MQ=255)
         atAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGttttt                                                                                                                                    >  1:1032851/2‑141 (MQ=255)
          tAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtttttt                                                                                                                                   <  2:341312/141‑1 (MQ=255)
          tAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGtttttt                                                                                                                                   <  1:580041/141‑1 (MQ=255)
           aaTTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTc                                                                                                                                  >  2:723027/1‑141 (MQ=255)
              ttcttcAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATa                                                                                                                               <  1:623210/141‑1 (MQ=255)
                cttcAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTGAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAg                                                                                                                             <  2:573195/141‑1 (MQ=255)
                cttcAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCATGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAg                                                                                                                             >  2:1057632/1‑141 (MQ=255)
                   cAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGCTTTTTCATAAGCaa                                                                                                                          >  1:669798/1‑141 (MQ=255)
                    aGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAg                                                                                                                         <  2:212170/141‑1 (MQ=255)
                     gCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGt                                                                                                                        <  2:93500/141‑1 (MQ=255)
                        tcatcaACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGtaat                                                                                                                     <  2:831276/141‑1 (MQ=255)
                           tcaACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTaataat                                                                                                                  >  1:745725/1‑141 (MQ=255)
                            caACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATc                                                                                                                 <  2:158757/141‑1 (MQ=255)
                             aaCATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATcc                                                                                                                <  1:33449/141‑1 (MQ=255)
                             aaCATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATcc                                                                                                                <  1:1013029/141‑1 (MQ=255)
                             aaCATCATAGTAAAGTAAGAATTTAACAGCATTTGCACCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCATTTACGTCATAACCAGTTTTTTCATAAGCTAGCAATAATcc                                                                                                                <  2:777823/141‑1 (MQ=255)
                               catcatAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCcac                                                                                                              >  2:281068/1‑141 (MQ=255)
                                atcatAGTAAAGTAAGAATTTAACAGCATTTGCACCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCATTTACGTCATAACCAGTTTTTTCATAAGCTAGCAATAATCcaca                                                                                                             <  1:1075293/141‑1 (MQ=255)
                                      gtaaagtaaGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCttt                                                                                                       <  1:762839/141‑1 (MQ=255)
                                          agtaaGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTa                                                                                                   <  1:686621/141‑1 (MQ=255)
                                            taaGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACg                                                                                                 >  2:383901/1‑141 (MQ=255)
                                                aaTTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCa                                                                                             >  1:930547/1‑141 (MQ=255)
                                                aaTTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCa                                                                                             <  1:1075825/141‑1 (MQ=255)
                                                aaTTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCa                                                                                             <  1:354411/141‑1 (MQ=255)
                                                 aTTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCAt                                                                                            <  1:304462/141‑1 (MQ=255)
                                                 aTTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCAt                                                                                            <  1:177949/141‑1 (MQ=255)
                                                 aTTTAACAGCATTTGCGCATTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTCTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCAt                                                                                            >  2:51180/1‑141 (MQ=255)
                                                   ttAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCt                                                                                          <  1:989356/141‑1 (MQ=255)
                                                       cAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAg                                                                                      <  1:416890/141‑1 (MQ=255)
                                                        aGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAATCGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCACGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGc                                                                                     <  1:782975/141‑1 (MQ=255)
                                                          cATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTg                                                                                   <  2:662952/141‑1 (MQ=255)
                                                              tGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTaa                                                                               >  1:1084087/1‑141 (MQ=255)
                                                               gcgcCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTaaa                                                                              <  1:809997/141‑1 (MQ=255)
                                                                cgcCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAAc                                                                             >  1:69968/1‑141 (MQ=255)
                                                                 gcCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATACGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAAcc                                                                            >  2:321370/1‑141 (MQ=255)
                                                                  ccTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATATTCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCa                                                                           <  2:934283/141‑1 (MQ=255)
                                                                   cTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTCTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCat                                                                          >  2:822026/1‑141 (MQ=255)
                                                                      tttCTTTTAAACTTTTTGCATACCACTCTACTAAACAGTTAAGTAAACAACCTTTAGCGTTTACGTCATATCCTGTTTTTTCATAATCAACTAATAATCCACAATCTCTCTTACGTGCATCTTAAGCTTGTAAACCATCtt                                                                       <  2:806180/140‑1 (MQ=255)
                                                                       ttCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTc                                                                      <  2:169423/141‑1 (MQ=255)
                                                                          ttttAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAgg                                                                   <  1:814271/141‑1 (MQ=255)
                                                                            ttAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATTATCACCAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTTAggtt                                                                 >  2:446899/1‑139 (MQ=255)
                                                                             tAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATc                                                                >  1:196096/1‑141 (MQ=255)
                                                                               aaCGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCta                                                              <  2:307001/141‑1 (MQ=255)
                                                                                   ttttGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCtaataa                                                          >  1:576961/1‑141 (MQ=255)
                                                                                    tttttataCCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATaaa                                                         >  2:494947/8‑141 (MQ=255)
                                                                                    tttGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTGATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATaaa                                                         >  2:575607/1‑141 (MQ=255)
                                                                                    tttGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATaaa                                                         <  2:22176/141‑1 (MQ=255)
                                                                                    tttGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATaaa                                                         <  1:777772/141‑1 (MQ=255)
                                                                                    tttGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATaaa                                                         <  1:777385/141‑1 (MQ=255)
                                                                                    tttGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATaaa                                                         <  1:1070689/141‑1 (MQ=255)
                                                                                       gcggACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAAtt                                                      >  1:545755/4‑141 (MQ=255)
                                                                                       gCAGACCACTCTACTAAACAGTCACGTAAACGACCTTTAGCGCTTACGTCATAACCAGTTTATTCATAAGCCAGCCATAATCCACAATCTTTGTTACGTGCATCTGAAGCATGTCAACCATATTCCGGATCTAATaaaact                                                      >  2:180435/1‑139 (MQ=255)
                                                                                        cAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAAtgg                                                     >  1:499883/1‑139 (MQ=255)
                                                                                        cAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATACCCAGTTTTTTCATAAGCAAGTAATAATCCCCAATCTTTGTTACGTGAATCTAAAGCTAGTAAACCATAATCCGTATATAATAAAAtta                                                     >  2:747088/1‑140 (MQ=255)
                                                                                              aCTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATg                                               >  2:371838/1‑141 (MQ=255)
                                                                                                tctACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCa                                             <  2:734322/141‑1 (MQ=255)
                                                                                                 ctactaAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCat                                            >  1:1036779/1‑141 (MQ=255)
                                                                                                  tactaAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTTTTATTCTAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCata                                           >  2:1001527/1‑141 (MQ=255)
                                                                                                  tactaAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCata                                           <  1:970694/141‑1 (MQ=255)
                                                                                                   actaAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCatat                                          >  2:275552/1‑141 (MQ=255)
                                                                                                    ctaAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATAtt                                         >  1:832665/1‑141 (MQ=255)
                                                                                                     taAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATtg                                        <  1:312154/141‑1 (MQ=255)
                                                                                                       aaCAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATtgtg                                      >  1:1008957/1‑141 (MQ=255)
                                                                                                             cAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAtt                                <  1:808500/141‑1 (MQ=255)
                                                                                                             cAGGTAAACGACCTTTAGCATTTACGTCATAACCAGTTTTTTCATAAGCTAGCAATAATCCACAATCTTTGTTACGTGCATCAGATGCTGGTAAACCGTATTCTGGATCTAATAAAATTGAAGACGCATATTGTGTTAAtt                                <  2:47317/141‑1 (MQ=255)
                                                                                                             cAGGTAAACGACCTTTAGCATTTACGTCATAACCAGTTTTTTCATAAGCTAGCAATAATCCACAATCTTTGTTACGTGCATCAGATGCTGGTAAACCGTATTCTGGATCTAATAAAATTGAAGACGCATATTGTGTTAAtt                                <  2:271272/141‑1 (MQ=255)
                                                                                                              aGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAttc                               >  1:316172/1‑141 (MQ=255)
                                                                                                              aGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAttc                               <  2:1014686/141‑1 (MQ=255)
                                                                                                               ggTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAttct                              >  1:898625/1‑141 (MQ=255)
                                                                                                               ggTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAttct                              <  2:929779/141‑1 (MQ=255)
                                                                                                                gTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAttctt                             >  2:201095/1‑141 (MQ=255)
                                                                                                                 taaacgctaaaggtcGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAAttcttc                            >  2:683352/15‑141 (MQ=255)
                                                                                                                     cGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGCTCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCa                        >  2:169796/1‑141 (MQ=255)
                                                                                                                     cGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCa                        >  2:487559/1‑141 (MQ=255)
                                                                                                                         cTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTa                    >  2:187545/1‑141 (MQ=255)
                                                                                                                          tttAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTaa                   <  1:21238/141‑1 (MQ=255)
                                                                                                                          tttAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTaa                   <  1:61995/141‑1 (MQ=255)
                                                                                                                             aGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATAc                <  1:494947/141‑1 (MQ=255)
                                                                                                                              gCGTTTACGTCAGATCCAGTTTTTTCCTAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACt               <  1:127389/141‑1 (MQ=255)
                                                                                                                               cGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACtt              >  1:907805/1‑141 (MQ=255)
                                                                                                                                  ttACGTCATATCTAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAATCTGGTAAACCATATTCAGGATCTGATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACTTTTa           >  2:525394/1‑141 (MQ=255)
                                                                                                                                  ttACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACTTTTa           >  2:368213/1‑141 (MQ=255)
                                                                                                                                    aCGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACTTTTAAt         >  1:439540/1‑141 (MQ=255)
                                                                                                                                     cGTCATAACCAGTTTTTTCATAAGCTAGCAATAATCCACAATCTTTGTTACGTGCATCAGATGCTGGTAAACCGTATTCTGGATCTAATAAAATTGAAGACGCATATTGTGTTAATTCTTCCGCAACCAATACTTTTAAtt        <  2:31098/141‑1 (MQ=255)
                                                                                                                                       tCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACTTTTAATTGt      >  1:418837/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTTTGCAGACCACTCTACTAAACAGTCAGGTAAACGACCTTTAGCGTTTACGTCATATCCAGTTTTTTCATAAGCAAGTAATAATCCACAATCTTTGTTACGTGCATCTGAAGCTGGTAAACCATATTCAGGATCTAATAAAATTGAAGATGCATATTGTGTTAATTCTTCCGCAACTAATACTTTTAATTGTTCAA  >  CP000730/2330558‑2330837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: