BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,338,910	T→A	6.4%	D225E (GAT→GAA) ‡	hysA →	hyaluronate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,338,910	0	T	A	6.4%	347.9 / 9.3	109	D225E (GAT→GAA) ‡	hysA	hyaluronate lyase
Reads supporting (aligned to +/- strand): ref base T (43/59); new base A (4/3); total (47/62)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCATTTAA  >  CP000730/2338772‑2339050

gAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAg                                                                                                                                            <  1:881713/141‑1 (MQ=255)

gAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAg                                                                                                                                            >  2:456540/1‑141 (MQ=255)

   tACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGa                                                                                                                                         <  1:822862/141‑1 (MQ=255)

      agctttAAATTGGTGGGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGatga                                                                                                                                      >  1:848318/5‑138 (MQ=255)

      agctttAAATTGGTGGGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGatga                                                                                                                                      >  1:888332/5‑138 (MQ=255)

           tAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAatat                                                                                                                                 <  1:1079072/141‑1 (MQ=255)

           tAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAatat                                                                                                                                 <  1:1079135/141‑1 (MQ=255)

             aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                                                                                                                               <  1:406517/141‑1 (MQ=255)

             aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                                                                                                                               <  2:1034856/141‑1 (MQ=255)

              aTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtattat                                                                                                                              <  1:522008/141‑1 (MQ=255)

                tggtggGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTAtct                                                                                                                            >  2:438495/1‑141 (MQ=255)

                  gtggGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTAtcttc                                                                                                                          >  1:751044/1‑141 (MQ=255)

                   tggGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTAtcttct                                                                                                                         <  1:101800/141‑1 (MQ=255)

                    gggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTg                                                                                                                        <  2:716206/141‑1 (MQ=255)

                     ggATTATGAAATTGGCACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGt                                                                                                                       <  1:902837/141‑1 (MQ=255)

                     ggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGt                                                                                                                       <  1:5580/141‑1 (MQ=255)

                     ggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGt                                                                                                                       >  2:728237/1‑141 (MQ=255)

                          aTGAAATTGGAACACCTAGATCATTAACAAATACGCTTGTATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGTAGGaa                                                                                                                  >  2:376482/1‑141 (MQ=255)

                            gAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGaaaa                                                                                                                >  2:846779/1‑141 (MQ=255)

                             aaaTTGGAACACCTAAATCATTAACAAATGCGCTTATATTGCTGAATGATGTAGGTTCAAATGAATAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg                                                                                                               <  1:539855/141‑1 (MQ=255)

                             aaaTTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg                                                                                                               <  2:581438/141‑1 (MQ=255)

                             aaaTTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg                                                                                                               <  1:527606/141‑1 (MQ=255)

                                  ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa                                                                                                          >  1:1076303/1‑141 (MQ=255)

                                  ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAAAAAAAAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa                                                                                                          <  2:631718/141‑1 (MQ=255)

                                   gAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAAc                                                                                                         >  2:228895/1‑141 (MQ=255)

                                     acacCTAGATCATAAAGACATACGCTTATATTTCTTAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACAATCAACAAAAAAAGTGATGAAATATTATCTTCTGTAGGAAAAGCTGAAcct                                                                                                       <  2:303878/141‑3 (MQ=255)

                                      cacCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTg                                                                                                      >  2:281965/1‑141 (MQ=255)

                                       acCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGc                                                                                                     <  1:710183/141‑1 (MQ=255)

                                       acCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAAAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGc                                                                                                     <  2:160487/141‑1 (MQ=255)

                                         cTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTa                                                                                                   <  1:752203/141‑1 (MQ=255)

                                               cATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCg                                                                                             >  2:355102/1‑141 (MQ=255)

                                               cATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCg                                                                                             <  1:219066/141‑1 (MQ=255)

                                               cATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCg                                                                                             <  1:845553/141‑1 (MQ=255)

                                                aTTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCgg                                                                                            >  1:121352/1‑141 (MQ=255)

                                                 ttgaCAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGa                                                                                           <  1:1043446/138‑1 (MQ=255)

                                                 ttAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGa                                                                                           >  1:871410/1‑141 (MQ=255)

                                                 ttAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGa                                                                                           <  2:184405/141‑1 (MQ=255)

                                                   aaCAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGaaa                                                                                         <  1:939519/141‑1 (MQ=255)

                                                      aaaTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCt                                                                                      <  1:85753/141‑1 (MQ=255)

                                                        aTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtag                                                                                    >  2:8953/1‑141 (MQ=255)

                                                        aTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtag                                                                                    <  1:326446/141‑1 (MQ=255)

                                                        aTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtag                                                                                    >  1:779129/1‑141 (MQ=255)

                                                          aCGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtagta                                                                                  >  1:117988/1‑141 (MQ=255)

                                                          aCGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGTAGGAAAAGCTGAACCTGCTAAAGGCGGAAATTtagta                                                                                  <  2:888332/141‑1 (MQ=255)

                                                            gCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCCATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGa                                                                                <  1:342890/141‑1 (MQ=255)

                                                            gCTTACATGGCTGAATGATCAATCTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTGCGCCCCAGATAGTGACAAAAGATTATCGTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGa                                                                                <  1:717859/141‑1 (MQ=255)

                                                             cTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGAc                                                                               <  1:35206/141‑1 (MQ=255)

                                                                     gCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTaa                                                                       >  1:616750/1‑141 (MQ=255)

                                                                     gCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTaa                                                                       >  2:275298/1‑141 (MQ=255)

                                                                       tGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAgg                                                                     >  1:42184/1‑141 (MQ=255)

                                                                         aaTGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTg                                                                   <  2:890996/141‑1 (MQ=255)

                                                                         aaTGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTg                                                                   <  1:846779/141‑1 (MQ=255)

                                                                           tGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGaa                                                                 >  2:896661/1‑141 (MQ=255)

                                                                             aTCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAAc                                                               <  2:607545/141‑1 (MQ=255)

                                                                                 aTTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGTCACGACTAAGGTGAAACtttt                                                           >  1:283238/1‑141 (MQ=255)

                                                                                 aTTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACtttt                                                           >  1:741932/1‑141 (MQ=255)

                                                                                   tttCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAg                                                         <  2:577004/141‑1 (MQ=255)

                                                                                    ttCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGa                                                        <  1:612411/141‑1 (MQ=255)

                                                                                     tCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGaa                                                       <  1:291568/141‑1 (MQ=255)

                                                                                        aaTGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGt                                                    <  1:877098/141‑1 (MQ=255)

                                                                                        aaTGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGt                                                    <  2:247475/141‑1 (MQ=255)

                                                                                        aaTGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGt                                                    >  1:897607/1‑141 (MQ=255)

                                                                                          tGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGtat                                                  >  1:561566/1‑141 (MQ=255)

                                                                                            aagaaaagaaAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGtatta                                                >  1:12736/1‑141 (MQ=255)

                                                                                                aaagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCga                                            >  1:698401/1‑141 (MQ=255)

                                                                                                aaagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCga                                            >  1:82030/1‑141 (MQ=255)

                                                                                                aaagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCga                                            >  1:443668/1‑141 (MQ=255)

                                                                                                 aagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCgaa                                           <  2:343206/141‑1 (MQ=255)

                                                                                                 aagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCgaa                                           <  2:1069465/141‑1 (MQ=255)

                                                                                                  agaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCgaag                                          >  1:937622/1‑141 (MQ=255)

                                                                                                       aaaTTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagat                                     >  2:502069/1‑141 (MQ=255)

                                                                                                       aaaTTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagat                                     <  1:317935/141‑1 (MQ=255)

                                                                                                        aaTTCACTGCCCCTATTCAAACTTTCGCCCCAGATAGTGACAAAATATTATCTAAAGTGGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagata                                    <  1:884774/141‑1 (MQ=255)

                                                                                                        aaTTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagata                                    <  2:1059126/141‑1 (MQ=255)

                                                                                                          ttCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaa                                  >  2:528467/1‑141 (MQ=255)

                                                                                                           tCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaag                                 <  2:131822/141‑1 (MQ=255)

                                                                                                             aCTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaagat                               >  2:704448/1‑141 (MQ=255)

                                                                                                             aCTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaagat                               >  1:501168/1‑141 (MQ=255)

                                                                                                             aCTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaagat                               >  1:508609/1‑141 (MQ=255)

                                                                                                                 ccccTATTAAAACTTTCGCCCCATATAGGGACAAAATATTATCGGCTGTAGGAAAAGCTGAACTTGCTAATGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATatga                           >  1:973530/1‑141 (MQ=255)

                                                                                                                 ccccTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATatga                           <  2:406400/141‑1 (MQ=255)

                                                                                                                    cTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTCGGAAAAGCTGAACTTGCTAAAGGCGACAATCTAGTAGCCATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATAtgatga                        <  1:270289/141‑1 (MQ=255)

                                                                                                                    cTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGCCCTTGCTAACGGCGGCAACCTAGCAGCCATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATAtgatga                        <  1:270276/141‑1 (MQ=255)

                                                                                                                      aTTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGaaa                      >  1:982162/1‑141 (MQ=255)

                                                                                                                         aaaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAg                   <  2:779129/141‑1 (MQ=255)

                                                                                                                          aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt                  >  2:46615/1‑141 (MQ=255)

                                                                                                                          aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt                  <  2:856908/141‑1 (MQ=255)

                                                                                                                          aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt                  >  2:349308/1‑141 (MQ=255)

                                                                                                                          aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt                  >  2:986849/1‑141 (MQ=255)

                                                                                                                            aCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCt                >  2:424338/1‑141 (MQ=255)

                                                                                                                              tttCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCtat              <  2:616750/141‑1 (MQ=255)

                                                                                                                              tttCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACAGTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCtat              <  1:983831/141‑1 (MQ=255)

                                                                                                                                tCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAg            >  1:588223/1‑141 (MQ=255)

                                                                                                                                 cGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGa           <  2:980770/141‑1 (MQ=255)

                                                                                                                                 cGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGa           >  1:193993/1‑141 (MQ=255)

                                                                                                                                     ccAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCa       <  1:231540/141‑1 (MQ=255)

                                                                                                                                     ccAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGAAATAATGAAAAAGTCTATAGATTCa       <  2:1076303/141‑1 (MQ=255)

                                                                                                                                      cAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCAt      <  1:1014252/141‑1 (MQ=255)

                                                                                                                                      cAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCAt      <  1:655380/141‑1 (MQ=255)

                                                                                                                                          tAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGAAATTTCTAAGGTGAAAATTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCATTtaa  <  1:923247/141‑1 (MQ=255)

GAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCATTTAA  >  CP000730/2338772‑2339050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`