Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,428,236 A→G 6.2% intergenic (‑139/+463) USA300HOU_2291 ← / ← lytR formate dehydrogenase alpha subunit/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,428,2360AG 6.2% 282.0 / 7.5 97intergenic (‑139/+463)USA300HOU_2291/lytRformate dehydrogenase alpha subunit/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (47/44);  new base G (3/3);  total (50/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

Only 100 of 102 total aligned reads displayed.

TATTACTCACCCCGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATCTTTC  >  CP000730/2428097‑2428376
                                                                                                                                           |                                                                                                                                             
tatTACTCACCCCGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTaa                                                                                                                                              <  1:875082/141‑1 (MQ=255)
     cTCACCCCGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTCGATAAAAttt                                                                                                                                         >  2:39882/1‑141 (MQ=255)
     cTCACCCCGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAGGTaggattg                                                                                                                                         >  2:40021/1‑135 (MQ=255)
     cTCACCCCGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAAttt                                                                                                                                         <  2:240285/141‑1 (MQ=255)
      tCACCCCGTCTATATATATTTCCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAAtttt                                                                                                                                        <  1:252511/141‑1 (MQ=255)
        cccccGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGt                                                                                                                                      <  1:689160/140‑1 (MQ=255)
            cGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTAttt                                                                                                                                  >  1:614395/1‑141 (MQ=255)
              tCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTg                                                                                                                                <  2:576979/141‑1 (MQ=255)
                 atatatatTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCt                                                                                                                             >  1:1055794/1‑141 (MQ=255)
                   atatatTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAGTTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCttt                                                                                                                           <  2:849/141‑1 (MQ=255)
                    tatatTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCtttt                                                                                                                          >  2:1006684/1‑141 (MQ=255)
                       atTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTAc                                                                                                                       <  2:295411/141‑1 (MQ=255)
                       atTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTAc                                                                                                                       <  2:397145/141‑1 (MQ=255)
                            ccGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCa                                                                                                                  <  2:876902/141‑1 (MQ=255)
                            ccGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCa                                                                                                                  <  2:876938/141‑1 (MQ=255)
                              gTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTATTTTATGTACTAGTTTTTAAGTAAAATTTTGTATTTTGCCTTTTTTcattcatt                                                                                                                >  1:165190/1‑141 (MQ=255)
                               tAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCAttt                                                                                                               <  1:911813/141‑1 (MQ=255)
                               tAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCAttt                                                                                                               <  1:1058802/141‑1 (MQ=255)
                               tAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCAttt                                                                                                               <  1:359952/141‑1 (MQ=255)
                               tAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCAttt                                                                                                               >  1:492006/1‑141 (MQ=255)
                                    gACTTTTAATAAATTGCTCATATCCACCTAAAATCACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTAtt                                                                                                          <  1:1007666/141‑1 (MQ=255)
                                      cTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTg                                                                                                        >  1:190273/1‑141 (MQ=255)
                                       ttttAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGa                                                                                                       >  1:133195/1‑141 (MQ=255)
                                       ttttAATAAATTGCTCATATCCACCTAAAATAACGATGCACAACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGTCTTTTTACAATAATTTTTATTTGa                                                                                                       >  2:717564/1‑141 (MQ=255)
                                                aTTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATAttttt                                                                                              >  2:493/1‑140 (MQ=255)
                                                aTTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTg                                                                                              >  1:841938/1‑141 (MQ=255)
                                                aTTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTg                                                                                              <  2:769093/141‑1 (MQ=255)
                                                 tttttCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATTTACTATTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTgc                                                                                             >  2:166652/5‑141 (MQ=255)
                                                 ttGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTgc                                                                                             >  2:525358/1‑141 (MQ=255)
                                                  tGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTGAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTgcg                                                                                            <  1:709649/141‑1 (MQ=255)
                                                   gCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTTGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTgcgc                                                                                           >  1:859934/1‑141 (MQ=255)
                                                   gCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTgcgc                                                                                           <  2:58063/141‑1 (MQ=255)
                                                    cTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGcgcg                                                                                          <  1:56995/141‑1 (MQ=255)
                                                     tCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGa                                                                                         >  1:61818/1‑141 (MQ=255)
                                                      gatatCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTGAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGaa                                                                                        >  1:1032590/2‑141 (MQ=255)
                                                      cATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTGAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGaa                                                                                        <  1:464675/141‑1 (MQ=255)
                                                      cATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGaa                                                                                        >  1:374976/1‑141 (MQ=255)
                                                       atatCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGaaa                                                                                       <  2:966024/141‑1 (MQ=255)
                                                             aCCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTACGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAat                                                                                 <  1:759154/141‑1 (MQ=255)
                                                              ccTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAatc                                                                                >  1:512486/1‑141 (MQ=255)
                                                               cTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAatca                                                                               >  1:47761/1‑141 (MQ=255)
                                                                tAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAatcat                                                                              >  1:810544/1‑141 (MQ=255)
                                                                tAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAatcat                                                                              <  2:365559/141‑1 (MQ=255)
                                                                   aaTAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCtt                                                                           >  2:732229/1‑141 (MQ=255)
                                                                   aaTAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCtt                                                                           <  1:656885/141‑1 (MQ=255)
                                                                    aTAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCttt                                                                          <  1:483254/141‑1 (MQ=255)
                                                                     tAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAACATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCtttt                                                                         <  1:377295/141‑1 (MQ=255)
                                                                     tAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCtttt                                                                         <  1:1023876/141‑1 (MQ=255)
                                                                     tAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCtttt                                                                         >  1:876694/1‑141 (MQ=255)
                                                                     tAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCtttt                                                                         <  1:406439/141‑1 (MQ=255)
                                                                        ccaTGACCCCCACCTCTTTCCGTTAGCATTGCTTTTCTTGTATTAATATATGGACTAGTTGTTAAGTAAAATTTTGTATTATGGCTATTTACACTCATTATTATTTGACATATTTTGGGCGCAATTAAATACTCTTTTTGt                                                                      <  2:840161/139‑1 (MQ=255)
                                                                        cGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGt                                                                      >  1:539336/1‑141 (MQ=255)
                                                                        cGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGt                                                                      >  1:1029603/1‑141 (MQ=255)
                                                                          aTGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTGAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTaaa                                                                                  <  2:963572/127‑1 (MQ=255)
                                                                          aTGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTGAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTaaa                                                                                  >  1:963572/1‑127 (MQ=255)
                                                                          aTGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGttt                                                                    >  2:153465/1‑141 (MQ=255)
                                                                             ccccACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAAt                                                                 >  2:579133/1‑141 (MQ=255)
                                                                                  cacaTCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGaaa                                                            <  2:865377/141‑1 (MQ=255)
                                                                                  cacaTCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGaaa                                                            >  1:706984/1‑141 (MQ=255)
                                                                                      tCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAaataa                                                        >  1:928907/1‑141 (MQ=255)
                                                                                       cTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAaataat                                                       >  2:176135/1‑141 (MQ=255)
                                                                                          tCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTAt                                                    >  2:277951/1‑141 (MQ=255)
                                                                                                 gAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTTTATTCTTTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCattatt                                             >  2:283528/1‑141 (MQ=255)
                                                                                                 gAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCattatt                                             >  1:384043/1‑141 (MQ=255)
                                                                                                 gAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCattatt                                             >  1:226596/1‑141 (MQ=255)
                                                                                                      aatttaattGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGttt                                       >  2:177191/1‑141 (MQ=255)
                                                                                                      aatttaattGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTAAAAAAAAAAAAAAATAATTATCATTATTAGttt                                       <  2:359786/141‑1 (MQ=255)
                                                                                                       atttaattGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGtttt                                      >  1:838754/1‑141 (MQ=255)
                                                                                                          taattGTATTACTTTATGTACTAGTTGTCAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCa                                   <  2:672781/141‑1 (MQ=255)
                                                                                                           aattGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCaa                                  >  2:967766/1‑141 (MQ=255)
                                                                                                            attGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAAt                                 <  1:82633/141‑1 (MQ=255)
                                                                                                             ttGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAAtt                                >  1:943908/1‑141 (MQ=255)
                                                                                                              tGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTa                               <  1:216984/141‑1 (MQ=255)
                                                                                                               gTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTAt                              <  2:635681/141‑1 (MQ=255)
                                                                                                                tATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATc                             >  2:669012/1‑141 (MQ=255)
                                                                                                                 aTTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCt                            <  2:588242/141‑1 (MQ=255)
                                                                                                                 aTTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCt                            >  1:352656/1‑141 (MQ=255)
                                                                                                                  ttACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTg                           <  2:821057/141‑1 (MQ=255)
                                                                                                                     cTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGttt                        >  1:947774/1‑141 (MQ=255)
                                                                                                                     cTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGttt                        >  2:452133/1‑141 (MQ=255)
                                                                                                                      tttATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTc                       >  1:675922/1‑141 (MQ=255)
                                                                                                                       ttATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCa                      <  1:297642/141‑1 (MQ=255)
                                                                                                                        tATGTACTAGTTGTTAAGTGAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCAc                     >  2:58129/1‑141 (MQ=255)
                                                                                                                        tATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACACTCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCAc                     <  1:212395/141‑1 (MQ=255)
                                                                                                                          tGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGc                   <  2:184667/141‑1 (MQ=255)
                                                                                                                              cTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCtttt               <  2:290350/141‑1 (MQ=255)
                                                                                                                              cTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCtttt               >  2:843128/1‑141 (MQ=255)
                                                                                                                               tAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCttttt              <  2:1055794/141‑1 (MQ=255)
                                                                                                                               tAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCttttt              <  1:249476/141‑1 (MQ=255)
                                                                                                                               tAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCttttt              <  1:337149/141‑1 (MQ=255)
                                                                                                                                aGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTg             <  2:11842/141‑1 (MQ=255)
                                                                                                                                 gttgttAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCAGTATTAGATTTTCTAATTATATGTTTCACGCTTTTTg             >  2:1011794/1‑141 (MQ=255)
                                                                                                                                   tgttAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCa          <  2:328156/141‑1 (MQ=255)
                                                                                                                                       aaGTAAAATTTTGTATTTTGCCTTTTTATAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATc      >  1:879537/1‑141 (MQ=255)
                                                                                                                                       aaGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATc      <  1:816366/141‑1 (MQ=255)
                                                                                                                                       aaGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATc      <  1:891633/141‑1 (MQ=255)
                                                                                                                                          tAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGATATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATCttt   >  2:695559/1‑141 (MQ=255)
                                                                                                                                          tAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATCttt   >  1:655201/1‑141 (MQ=255)
                                                                                                                                           aaaaTTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATCTTTc  <  1:795993/141‑1 (MQ=255)
                                                                                                                                           aaaaTTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATCTTTc  >  1:233740/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
TATTACTCACCCCGTCTATATATATTTTCCGTAAATGACTTTTAATAAATTGCTCATATCCACCTAAAATAACGATGCCCCACACATCTTTCAGATAGAATTAATTTAATTGTATTACTTTATGTACTAGTTGTTAAGTAAAATTTTGTATTTTGCCTTTTTACAATCATTTTTATTTGAAATATTTTGCGCGAAATTAAATCATCTTTTTGTTTAATTGAAAATAATTATCATTATTAGTTTTCCAATTATCTGTTTCACGCTTTTTGCCATATCTTTC  >  CP000730/2428097‑2428376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: